Karakteristik sisik ikan nila merah sebagai bahan baku kolagen

Characteristics of red tilapia fish scales as raw materials for the collagen industry

Authors

DOI:

https://doi.org/10.17844/jphpi.v28i6.50129

Keywords:

alanine, glycine, green industry, Oreochromis niloticus, proline

Abstract

Fishery by-products can be used in the industry, for example, tilapia fish products, namely scales, bones, and heads, as a source of raw material for the collagen industry. This study aimed to determine the characteristics of red tilapia scales as raw materials for collagen. The fish scales were sun-dried for 2 days, and the dried scales were characterized and used to extract collagen. The analysis included yield calculations, proximate analysis, heavy metals, and amino acids from red tilapia fish scales. Red tilapia scales around 3.73±0.01%, head 24.91±0.03%, gills 4.32±0.01%, bones 19.19±0.02%, meat and skin 33.00±0.04% and viscera 14.85±0.01%. The chemical composition of red tilapia fish scales was 14.35 ± 0.09% water, 36.51±0.47% ash, 0.02±0.02% fat, 47.69±0.37% carbohydrate, 1.46±0.01% protein, and 196.52 kcal/100 g total energy content. Red tilapia scales also contain glycine (101,488.45±305.71 mg/g), proline (60,531.03±170.96 mg/g), and alanine (41,427.985±111.66 mg/g) as markers of collagen. Heavy metals such as arsenic, cadmium, and mercury were not detected, and lead was detected at 0.24 mg/kg. The characteristics of tilapia fish still contain components that can be used as raw materials in the collagen industry. The yield of collagen red tilapia was 14.70±3.25%. In the future, it is hoped that this product can be processed and further developed into raw materials for other industries to create a green industry.

References

Ahmad, M. G., Setyaningsih, I., & Trilaksani, W. (2019). Formulasi dan bioaktivitas suplemen tablet berbasis Spirulina dan hidrolisat kolagen kulit ikan nila. Jurnal Pengolahan Hasil Perikanan Indonesia. 22(3), 453-463. https://doi.org/10.17844/jphpi.v22i3.28925

Akter, S., Rahman A, M., Naher, J., Majumder, M. W. R., & Alam A. K. M. N. (2017). Fish glue from tilapia scale and skin and its physical and chemical characters. International Journal of Fisheries and Aquatic Studies, 5(2), 255-257.

Anggraen, N., Darmanto, Y. S., & Riyadi, P. H. (2016). Pemanfaatan nanokalsium tulang ikan nila (Oreochromis niloticus) pada beras analog dari berbagai macam ubi jalar (Ipomoea batatas L.). Jurnal Aplikasi Teknologi Pangan, 5(4), 114-122.

Apri, N., Fahrizal., & Novita, M. 2017. Isolation of fish skin and bone gelatin from tilapia (Oreochromis niloticus): Response surface approach. IOP Conf. Series: Materials Science and Engineering 334 (2018) 012061. DOI 10.1088/1757-899X/334/1/012061

Amiati, A., Fuadi, N., Nurrahmi, S., & Jumardin, J. 2024. Effect of gamma rays irradiation of cesium-137 on collagen extraction of nile fish scales (Oreochromis niloticus). Journal of Science and Technology, 13(2),6-12. https://doi.org/10.22487/25411969.2024.v13.i2.16676

Asjun A., Anwar, D. P., & Saleh, A. M,. (2024). Karakteristik nanokalsium food grade limbah sisik dan tulang ikan bandeng (Chanos chanos) Fabricius 1775 dan aplikasinya pada cookies. Jurnal Fish Protech, 7(2), 112-123

Basu, B. R., Banik, A. K., & Das, M. (2008). Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale. World Journal Microbiology Biotechnology, 24, 449-455. https://doi.org/10.1007/s11274-007-9492-6

Boran, G., & Regenstein, J. M. (2010). Fish Gelatin. In Advances in Food and Nutrition Research; Taylor S.L. Academic Press: Burlington, UK.

[BSN] Badan Standardisasi Nasional. Standar Nasional Indonesia 7387-2009. (2009). Batas maksimum cemaran logam berat dalam pangan. Jakarta (ID): Badan Standarisasi Nasional.

[BSN] Badan Standardisasi Nasional. Standar Nasional Indonesia 8076-2014. (2014). Kolagen kasar dari sisik ikan – Syarat mutu dan pengolahan. Jakarta (ID): Badan Standarisasi Nasional.

Carolina, D. N., Satari, M. H., Priosoeryanto, B. P., Susanto, A., & Sukotjo, C. (2024). Exploring carp scales (Cyprinus carpio L.) as a novel source of collagen for dental use: Extraction and characterization. Journal of Applied Pharmaceutical Science 14(04), 204-209. 10.7324/JAPS.2024.166542

Chen, J., Li, L., Yi, R., Xu, N., Gao, R., & Hong, B. (2016). Extraction and characterization of acid-soluble collagen from scales and skin of tilapia (Oreochromis niloticus). LWT - Food Science and Technology, 66, 453-459. https://doi.org/10.1016/j.lwt.2015.10.070

EL-Rashidy, A. A., Gad, A. Y., Abu-Hussein, A. E. H. G., & Habib, S. I. (2015). Chemical and biological evaluation of Egyptian nile tilapia (Orechromis niloticus) fish scale collagen. International Journal of Biological Macromolecules, 79(6), 18-26. https://doi.org/10.1016/j.ijbiomac.2015.05.019

Dewantoro, A. A., Kurniasih, R. A., & Suharto, S, (2019). Aplikasi gelatin sisik ikan nila (Oreochromis niloticus) sebagai pengental sirup nanas. Jurnal Ilmu dan Teknologi Perikanan. 1(1), 37-46. https://doi.org/10.14710/jitpi.2019.5246.

Dewi, F. R. (2021). Extraction and characterization of nano calcium from tilapia (Oreochromis niloticus) scales from Semarang, Indonesia. IOP Conf. Series: Earth and Environmental Science 924 (2021) 012086, 1-7, doi:10.1088/1755-1315/924/1/012086

Farid, A, J., Riyadi, P, H., Amalia, U,. (2015). Karakteristik kulit samak ikan nila (Oreochromis niloticus) dengan penambahan bating agent alami dari pankreas sapi. Jurnal Saintek Perikanan 10(2), 80-83. https://doi.org/10.14710/ijfst.10.2.80-83

FAO-GLOBEFISH. (2021) Tilapia Sector Growth to Resume After Shaking of Pandemic Effects. https://www.fao.org/in-action/globefish/market-reports/resourcedetail/en/c/ 1416636/

Gusmawan, R. A., Agustini, T. W., & Fahmi, A. A. (2020). Efek penambahan bio-calcium powder tulang ikan nila (Oreochromis niloticus) dengan konsentrasi berbeda terhadap karakteristik cookies berbahan dasar tepung mocaf. Jurnal Ilmu dan Teknologi Perikanan. 2(2), 22-30. https://doi.org/10.14710/jitpi.2020.9637

Handoko, F. (2020). Green Industrial System (Pendekatan baru dalam meningkatkan daya saing). Penerbit MK Press.

Huang, C. Y., Kuo, J. M., Wu, S. J., & Tsai, H. T. (2016). Isolation and characterization of fish scale collagen from tilapia (Oreochromis sp.) by a novel extrusion-hydro-extraction process. Food Chemistry, 190, 997-1006. https://doi.org/10.1016/j.foodchem.2015. 06.066

Istiqomah, R. W., & Sugiharto, A. (2023). Pemanfaatan gelatin dari tulang ikan nila (Oreochromis Niloticus) dengan metode asam sebagai pengental sirup. Jurnal Pengolahan Pangan. 8(1), 9-14. https://doi.org/10.31970/pangan.v8i1.87

Kuwahara, J. (2021). Extraction of type I collagen from tilapia scales using acetic acid and ultrafine bubbles. Processes, 9 (288), 1-11. https://doi.org/10.3390/pr9020288

Gusmawan, R. A., Agustini, T. W., Fahmi, A. S. (2020). Efek penambahan bio-calcium powder tulang ikan nila (Oreochromis niloticus) dengan konsentrasi berbeda terhadap karakteristik cookies berbahan dasar tepung mocaf. Jurnal Ilmu dan Teknologi Perikanan 2 (2), 22-30.

Lamima, A. R. (2022). Karakteristik gelatin dari sisik ikan nila (Oreochromis niloticus). [Skripsi]. Institut Pertanian Bogor.

Martins, M. E. O., Sousaa, J. R, Claudinoa, R. L., Lino, S. C. O., do Valea, D. A., Silva, A. L. C., Morais J. P. S., Filho, M. D. S. M. S., & De Souza, B. W. S. (2018). Thermal and chemical properties of gelatin from tilapia (Oreochromis niloticus) scale. Journal of Aquatic Food Product Technology, 27(10), 1120–1133. https://doi.org/10.1080/ 10498850.2018.153553

Mufida, S. N., & Herdyastuti, N. (2022). Ekstraksi gelatin sisik ikan nila (Oreochromis spp.) dengan variasi konsentrasi asam sitrat dan waktu demineralisasi. Jurnal Sumberdaya Akuatik Indopasifik, 6(3), 193-204. https://doi.org/10.46252/jsai-fpik-unipa.

Naidoo, D., Onwubu, S. C., Mokhothu, T. H., Mdluli, P. S., Makgobole, M. U., & Mishra, A. K. (2024). Effectiveness of Fish Scale-Derived Collagen as an Alternative Filler Material in the Fabrication of Polyurethane Foam Composites. Advances in Polymer Technology, 2024(1): 1723927. https://doi.org/10.1155/2024/1723927

Núñez-Tapia, I., Macouzet-Garduño, J., Ramírez-Ruiz, F., Vázquez-Vázquez, F. C., Álvarez-Pérez, M. A., Bucio-Galindo, L., & Piña-Barba, M. C. (2025). Bio-based composite membranes from fish scales: A novel approach to harnessing collagen and hydroxyapatite for tissue engineering applications. Biomedical Engineering Advances 9(2025): 100146. https://doi.org/10.1016/j.bea.2025.100146.

Nurhidayah, B., Soekendarsi, E. & Erviani, A. E. (2019). Kandungan kolagen sisik ikan bandeng Chanos-chanos dan sisik ikan nila Oreochromis niloticus. Jurnal Biologi Makasar, 4(1), 39-47. https://doi.org/10.20956/bioma.v4i1.6341

Nurimala, M., Suryamarevita, H., Hizbullah, H. H., Jacoeb, A. M., & Ochiai, Y. (2022). Fish skin as a biomaterial for halal collagen and gelatin. Saudi Journal of Biological Sciences, 29(7), 1100–1110. https://doi.org/10.1016/j.sjbs.2021.09.056

Peng, J., Zi, Y., Xu, J., Zheng, Y., Huang, S., Hu, Y., Liu, B., Wang, X., & Zhong, J. (2022). Effect of extraction methods on the properties of tilapia scale gelatins. International Journal of Biological Macromolecules, 221, 1150-1160. https://doi.org/10.1016/ j.ijbiomac. 2022.09.094

Prastyo, D. T., Trilaksani, W., & Nurjanah. (2020). Aktivitas antioksidan hidrolisat kolagen kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 423-433. https://doi.org/10.17844/jphpi.v23i3.31732

Romadhon, Darmanto, Y. S., & Kurniasih, R. A. (2019). Karakteristik kolagen dari tulang, kulit, dan sisik ikan nila. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(2), 403-410. https://doi.org/10.17844/jphpi.v22i2.28832

Rosmawati, Abustam, E., Tawali, A. B., Said, M. I., & Sari, D. K. (2018). Effect of body weight on the chemical composition and collagen content of snakehead fish Channa striata skin. Fisheries Science, 84(6), 1081–1089. https://doi.org/10.1007/s12562-018-1248-8

Sari, D. K., Darmanto, Y. S., Amalia, U. (2017). Pemanfaatan limbah hasil perikanan: lem ikan berbahan baku sisik ikan yang berbeda. Jurnal Ilmu Pangan dan Hasil Pertanian, 1(2), 60-71.

Setiawan, A., Riyadi, P, Sumardianto. (2015). Pengaruh penggunaan gambir (Uncaria gambier) sebagai bahan penyamak pada proses penyamakan kulit terhadap kualitas fisik kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 4(2), 124-132. https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/9202

Shiao, W. C., Wu, T. C., Kuo, C. H., Tsai, Y. H., Tsai, M. L., Hong, Y. H., & Huang, C. Y. (2021). Physicochemical and antioxidant properties of gelatin and gelatin hydrolysates obtained from extrusion-pretreated fish (Oreochromis sp.) scales. Marine Drugs, 19, 1-18. https://doi.org/10.3390/md19050275

Sukardi, R. W. I., & Sugiharto, A. (2023). Pemanfaatan gelatin dari tulang ikan nila (Oreochromis Niloticus) dengan metode asam sebagai pengental sirup. Jurnal Pengolahan Pangan, 8(1), 9-14. https://doi.org/10.31970/pangan.v8i1.87

Suleman, R., Zahoor, M. A., Qarni, M. A., Saleh, I. A., Rao, W., Hussain, M., Ismail, T., Akhtar, S., Okla, M. K., Alaraidh, I. A., AbdElgayed, G., Saud, S., Hassan, S., Nazwa, T., & Fahad, S. (2025). Assessment of heavy metals and microbial loads in Nile tilapia (Oreochromis niloticus) from different farms and rivers. Scientific Reports, 15:505, https://doi.org/10.1038/s41598-025-87152-y

Sockalingam, K., & Abdullah, H. Z. (2015). Extraction and characterization of gelatin biopolymer from black tilapia (Oreochromis mossambicus) scales. Proceedings of the 23rd Scientific Conference of Microscopy Society Malaysia (SCMSM 2014). AIP Conf. Proc. 1669, 020053 (2015). https://doi.org/10.1063/1.4919191

Syadeto, H. S., Sumardianto., & Purnamasari, L. (2017). Fortifikasi tepung tulang ikan nila (Oreochromis niloticus) sebagai sumber kalsium dan fosfor serta mutu cookies. Jurnal Ilmiah Teknosains, 3(1), 17-21. https://doi.org/10.26877/jitek.v3i1/%20Mei.1387

Syandri, H., Azrita, A., Mardiah, A., Aryani, N., & Diharmi, A. (2023). The proximate composition, amino acid profile, fatty acid content, and mineral content of scale flour from three fish species as potential feeds for fish fry. F1000Research, 12:1144, 1-19, 10.12688/f1000research.141304.2

Tang, C., Zhou, K., Zhu, Y., Zhang, W., Xie, Y., Wang, Z., Zhou, H., Yang, T., Zhang, Q., Xu, B. (2022). Collagen and its derivatives: from structure and properties to their applications in food industry. Food Hydrocolloids, 131(2022), 1-19. https://doi.org/ 10.1016/j.foodhyd.2022.107748

Tarigan, N., & Sitohang, U. (2024). Potensi tulang ikan nila (Oreochromis niloticus) untuk memenuhi kecukupan kalsium. Amerta Nutrition 8 (3SP), 372-379. https://doi.org/10.20473/amnt.v8i3SP.2024.37

Widiyanto, Uju, & Nurilmala, M. (2022). Karakteristik kolagen dari kulit dan sisik ikan coklatan, swanggi, dan kurisi sebagai bahan gelatin. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 512-527. http://dx.doi.org/10.17844/jphpi.v25i3.43598

Published

2025-07-03

How to Cite

Pertiwi, . R. M., Nurilmala, . M., Nurjanah, . N., & Nurhayati, . T. (2025). Karakteristik sisik ikan nila merah sebagai bahan baku kolagen : Characteristics of red tilapia fish scales as raw materials for the collagen industry . Jurnal Pengolahan Hasil Perikanan Indonesia, 28(6), 546-558. https://doi.org/10.17844/jphpi.v28i6.50129