Pemanfaatan insang ikan tuna (Thunnus sp.) dan lemuru (Sardinella lemuru) sebagai bahan baku pengolahan keripik

Utilization of tuna (Thunnus sp.) and Bali sardinella (Sardinella lemuru) fish gills as raw materials for chips processing

Authors

DOI:

https://doi.org/10.17844/jphpi.v28i6.63268

Keywords:

by-product, food safety, heavy metal, microbe, proximate

Abstract

Fish gills are low-value by-products of the fisheries industry; however, they are rich in nutrients and have the potential to be developed into value-added products, such as chips. However, as gills act as filters for chemical substances, including heavy metals, food safety is a critical concern. This study aimed to evaluate and compare the characteristics of tuna and Bali sardinella gills in fresh and frozen conditions as raw materials for chips in terms of proximate composition, heavy metal content, microbiological safety, and consumer acceptance. Four treatments were tested: fresh and frozen gills from both tuna and Bali sardinella (duplicates). Proximate analysis included moisture, ash, fat, and protein; heavy metals analysed were Hg, Pb, and Cd. Microbiological tests included total plate count (TPC), Escherichia coli, Salmonella sp., and Vibrio cholerae. Consumer acceptance was evaluated using a hedonic test based on sensory attributes. Raw gills contained 14.15–17.62% protein, 1.61–5.79% fat, 65.36–69.19% moisture, and 10.81–14.93% ash. After processing, the protein content decreased to 8.04–9.52%, whereas the fat content increased to 19.33–26.75%. Moisture and ash also declined to 4.12–4.67% and 4.66–6.19%, respectively. Fresh Bali sardinella gill chips had the highest fat content (26.75%), whereas frozen tuna gill chips had the highest protein content (9.52%). Heavy metal levels remained below the limits set by SNI 7387:2009, and all microbiological parameters complied with the food safety standards. Hedonic testing showed high consumer acceptance, with all products scoring above 8.00 overall, and the highest taste score (8.90) was recorded for frozen tuna gill chips.

References

Adharani, N., Kurniawati, A., Sulistiono, S., & Wardhana, M. G. (2017). Upaya minimalisasi dampak pencemaran dari limbah lemuru sebagai bahan baku nata de fish di Kecamatan Muncar Kabupaten Banyuwangi. Jurnal Enggano, 2(1), 1–10. https://doi.org/10.31186/jenggano.2.1.1-10

Afifah, A., Suparno, O., Haditjaroko, L., Tarman, K., Setiyono, A., & Nugraha, A. W. (2024). Isolation and characterization of collagen from salmon (Salmo salar) skin using papain enzyme. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(6), 536-552. http://dx.doi.org/10.17844/jphpi.v27i6.53285

Ahmed, T., Sharmin, A., Paul, A., Dipu, M. R., & Islam, T. (2020). Microbiological quality analysis of different types of popular dried food items. Food Research, 4(4), 1297–1302. https://doi.org/10.26656/FR.2017.4(4).080

Ali, M. M., Ali, M. L., Islam, M. S., & Rahman, M. Z. (2016). Preliminary assessment of heavy metals in water and sediment of Karnaphuli River, Bangladesh. Environmental Nanotechnology, Monitoring and Management, 5, 27–35. https://doi.org/10.1016/j.enmm.2016.01.002

Al-Jumayi, H. A., & Darwish, A. M. G. (2021). Frying time and temperature conditions’ influences on physicochemical, texture, and sensorial quality parameters of barley-soybean chips. Journal of Food Quality, 2021, 1–11. https://doi.org/10.1155/2021/5748495

Alsafy, M. A. M., Abd-Elhafeez, H. H., Rashwan, A. M., Erasha, A., Ali, S., & El-Gendy, S. A. A. (2025). Anatomy, histology, and morphology of fish gills in relation to feeding habits: a comparative review of marine and freshwater species. BMC Zoology, 10(1), 1–21. https://doi.org/10.1186/s40850-025-00223-5

Alsafy, M. A. M., El-Gendy, S. A. A., El-Bakary, N. E. R., Kamal, B. M., Derbalah, A., & Roshdy, K. (2023). Morphological comparison of the detailed structure of gill rakers from three different feeding habits of marine fish species. Zoomorphology, 142(1), 87–97. https://doi.org/10.1007/s00435-022-00574-4

[AOAC] AOAC International. (2016). Official Methods of Analysis of AOAC International. In 20th ed. Gaithersburg: AOAC. Association of Official Analytical Chemists.

Ayanda, I. O., Ekhator, U. I., & Bello, O. A. (2019). Determination of selected heavy metal and analysis of proximate composition in some fish species from Ogun River, Southwestern Nigeria. Heliyon, 5(10), 1–6. https://doi.org/10.1016/j.heliyon.2019.e02512

Aydin, I., & Gokoglu, N. (2014). Effects of temperature and time of freezing on lipid oxidation in anchovy (Engraulis encrasicholus) during frozen storage. European Journal of Lipid Science and Technology, 116(8), 1–6. https://doi.org/10.1002/ejlt.201300450

[BPOM] Badan Pengawas Obat dan Makanan. (2022). Peraturan Badan Pengawas Obat dan Makanan Nomor 9 Tahun 2022 tentang Persyaratan Cemaran Logam Berat dalam Pangan Olahan. Jakarta: Badan Pengawas Obat dan Makanan.

[BSN] Badan Standardisasi Nasional. (2006a). SNI 01-2332.2-2006 – Cara Uji Mikrobiologi – Bagian 2: Penentuan Salmonella pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2006b). SNI 01-2354.4-2006 – Cara Uji Kimia – Bagian 4: Penentuan Kadar Protein dengan Metode Total Nitrogen pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2007). SNI 01-2332.4-2006 – Cara Uji Mikrobiologi – Bagian 4: Penentuan Vibrio cholerae pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2009). SNI 7387:2009 – Batas Maksimum Cemaran Logam Berat dalam Pangan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2010). SNI 2354.1:2010 – Cara Uji Kimia – Bagian 1: Penentuan Kadar Abu dan Abu Tak Larut dalam Asam pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2011). SNI 2354.5:2011 – Cara Uji Kimia – Bagian 5: Penentuan Kadar Logam Berat Timbal (Pb) dan Kadmium (Cd) pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2015a). SNI 2332.1:2015 – Cara Uji Mikrobiologi – Bagian 1: Penentuan Koliform dan Escherichia coli pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2015b). SNI 2332.3:2015 – Cara Uji Mikrobiologi – Bagian 3: Penentuan Angka Lempeng Total (ALT) pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2015c). SNI 2354.2:2015 – Penentuan Kadar Air pada Produk Perikanan. In Badan Standardisasi Nasional (BSN) (SNI 2354.2:2015). Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2016). SNI 2354.6:2016 – Cara Uji Kimia – Bagian 6: Penentuan Kadar Logam Berat Merkuri (Hg) pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2017). SNI 2354-3:2017 – Cara Uji Kimia – Bagian 3: Penentuan Kadar Lemak Total pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2018). SNI 2346:2018 – Cara Uji Organoleptik pada Produk Perikanan. Jakarta: Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2021). SNI 2729:2021 – Ikan Segar. Jakarta: Badan Standardisasi Nasional.

Benyahia-Mostefaoui, A., Dehiba, F., Boualga, A., Taleb-Senouci, D., & Lamri-Senhadji, M. (2014). Milk lipids and sardine oil intake modulate differently enzymatic antioxidant defense in rats fed an atherogenic diet. Journal of Experimental and Integrative Medicine, 4(2), 115–121. https://doi.org/10.5455/jeim.060414.or.100

Bija, S., Luthfiyana, N., & Rozi, A. (2022). The effect of cooking process on nutritional composition of lais fish (Cryptopterus sp.). IOP Conference Series: Earth and Environmental Science, 1083(1), 1–10. https://doi.org/10.1088/1755-1315/1083/1/012012

Bordin, K., Kunitake, M.T., Aracava, K.K., Trindade, C.S.F. (2013). Changes in food caused by deep fat frying – a review. Alan Archivos Latinoamericanos de Nutricion, 63(1).

Budiadnyani, I. G. A., Cesrany, M., Ginting, E. K., & Sudaryatma, P. E. (2024). Organoleptic testing and histopathological examination of gills of Thunnus sp. and Sardinella lemuru as raw materials for gill chips. BIO Web of Conferences, 92, 1–7. https://doi.org/10.1051/bioconf/20249202003

Dawson, P., Al-Jeddawi, W., & Remington, N. (2018). Effect of freezing on the shelf life of salmon. International Journal of Food Science, 2018, 1–12. https://doi.org/10.1155/2018/1686121

Domingues, V. F., Quaresma, M., Sousa, S., Rosas, M., Ventoso, B., Nunes, M. L., & Delerue-Matos, C. (2021). Evaluating the lipid quality of yellowfin tuna (Thunnus albacares) harvested from different oceans by their fatty acid signatures. Foods, 10(11), 1–9. https://doi.org/10.3390/foods10112816

Evans, A. N., & Nunez, B. S. (2015). Fresh water acclimation elicits a decrease in plasma corticosteroids in the euryhaline atlantic stingray, dasyatis sabina. General and Comparative Endocrinology, 222, 167–172. https://doi.org/10.1016/j.ygcen.2015.08.010

Faciu, M. E., Lazar, I., Ifrim, I., Ureche, C., & Lazar, G. (2014). Exploratory spatial data analysis of heavy metals concentration in two sampling sites on Siret River. Environmental Engineering and Management Journal, 13(9), 2179–2186. https://doi.org/10.30638/eemj.2014.242

Fan, L., Xian, C., Tang, S., Ding, W., Xu, C. H., & Wang, X. C. (2022). Effect of frozen storage temperature on lipid stability of hepatopancreas of Eriocheir sinensis. LWT, 154, 1–9. https://doi.org/10.1016/j.lwt.2021.112513

Foyle, K. L., Hess, S., Powell, M. D., & Herbert, N. A. (2020). What is gill health and what is its role in marine finfish aquaculture in the face of a changing climate? In Frontiers in Marine Science, 7, 1–16. https://doi.org/10.3389/fmars.2020.00400

Franco, D., Munekata, P. E. S., Agregán, R., Bermúdez, R., López-Pedrouso, M., Pateiro, M., & Lorenzo, J. M. (2020). Application of pulsed electric fields for obtaining antioxidant extracts from fish residues. Antioxidants, 9(2), 1–14. https://doi.org/10.3390/antiox9020090

Ghaly, A. E., Ramakrishnan, V. V., Brooks, M. S., Budge, S. M., & Dave, D. (2013). Fish processing wastes as a potential source of proteins, amino acids and oils: a critical review. Journal of Microbial and Biochemical Technology, 5(4), 107–129. https://doi.org/10.4172/1948-5948.1000110

Gökoğlu, N., & Yerlikaya, P. (2015). Seafood chilling, refrigeration and freezing: science and technology. In Seafood Chilling, Refrigeration and Freezing: Science and Technology. Wiley-Blackwell. https://doi.org/10.1002/9781118512210

Habarakada, A., Perumpuli, P. A. B. N., Thathsaranee, W. T. V., & Wanninaika, I. P. (2021). Physical, chemical, and nutritional quality parameters of three different types of oil: determination of their reusability in deep frying. Food Research, 5(5), 226–235. https://doi.org/10.26656/FR.2017.5(5).079

Hallier, A., Chevallier, S., Serot, T., & Prost, C. (2008). Freezing-thawing effects on the colour and texture of European catfish flesh. International Journal of Food Science and Technology, 43(7), 1253–1262. https://doi.org/10.1111/j.1365-2621.2007.01601.x

Jumiati, J., Rahmaningsih, S., & Sudianto, A. (2021). Mutu kerupuk limbah insang ikan kurisi (Nemipterus japonicus) ditinjau dari analisis proksimat. Jurnal Teknologi Pangan, 15(1), 1–11. https://doi.org/10.33005/jtp.v15i1.2715

Kamila, F.R., Sarastani, D. (2025). Pengaruh metode thawing terhadap mutu produk naget ikan. Jurnal Miyang, 5(1), 9-15.

Kandyliari, A., Mallouchos, A., Papandroulakis, N., Golla, J. P., Lam, T. K. T., Sakellari, A., Karavoltsos, S., Vasiliou, V., & Kapsokefalou, M. (2020). Nutrient composition and fatty acid and protein profiles of selected fish by-products. Foods, 9(2), 1–14. https://doi.org/10.3390/foods9020190

Lestari, V., Kusumaningrum, I., Zuraida, I., Diachanty, S., & Pamungkas, B. F. (2024). Pemanfaatan kepala dan tulang ikan bawis (Siganus canaliculatus) pada pengolahan kerupuk. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(1), 16-26. http://dx.doi.org/10.17844/jphpi.v27i1.45014

Mangano, V., Gervasi, T., Rotondo, A., De Pasquale, P., Dugo, G., Macrì, F., & Salvo, A. (2021). Protein hydrolysates from anchovy waste: purification and chemical characterization. Natural Product Research, 35(3), 399–406. https://doi.org/10.1080/14786419.2019.1634711

Martínez-Alvarez, O., Chamorro, S., & Brenes, A. (2015). Protein hydrolysates from animal processing by-products as a source of bioactive molecules with interest in animal feeding: A review. Food Research International, 73, 204–212. https://doi.org/10.1016/j.foodres.2015.04.005

Maulidiyanti, E. T. S., Purwaningsih, N. V., Widiyastuti, R., Samsudin, R. R., & Arimurti, A. R. R. (2021). The effect of storage time for pooled sera on freezers on the quality of clinical chemical examination. Medicra (Journal of Medical Laboratory Science/Technology), 4(2), 78–82. https://doi.org/10.21070/medicra.v4i2.1613

Mozzon, M., Foligni, R., Mannozzi, C., & Vittori, S. (2024). Assessment of lipid oxidation in fish and fish products processed by cold plasma technologies. Applied Food Research, 4(2), 1–16. https://doi.org/10.1016/j.afres.2024.100646

Nawaz, A., Xiong, Z., Li, Q., Xiong, H., Irshad, S., Chen, L., Wang, P., Zhang, M., Hina, S., & Regenstein, J. M. (2019). Evaluation of physicochemical, textural and sensory quality characteristics of red fish meat-based fried snacks. Journal of the Science of Food and Agriculture, 99(13), 5771-5777. https://doi.org/10.1002/jsfa.9845

Nurhayati, N., & Peranginangin, R. (2009). Prospek pemanfaatan limbah perikanan sebagai sumber kolagen. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, 4(3), 83–92. https://doi.org/10.15578/squalen.v4i3.157

Olsen, R. L., Toppe, J., & Karunasagar, I. (2014). Challenges and realistic opportunities in the use of by-products from processing of fish and shellfish. In Trends in Food Science and Technology, 36(2), 144–151. https://doi.org/10.1016/j.tifs.2014.01.007

Pawar, P. P., Pagarkar, A. U., & Rathod, N. B. (2020). Effect of chilled storage on quality characteristics of battered and breaded snack product from large sized catla (Catla catla). Journal of Food Science and Technology, 57(1), 52-59. https://doi.org/10.1007/s13197-019-04028-6

Priyosetyoko, P., Widayat, W., Suzery, M., & Agustini, T. W. (2022). Potential of solid waste conversion into gelatin in the fisheries industry of indonesia. Indonesian Journal of Halal, 5(2), 112–139. https://doi.org/https://doi.org/10.14710/halal.v5i2.16719

Rosiani, N., Basito, B., & Widowati, E. (2015). Kajian karakteristik sensoris fisik dan kimia kerupuk fortifikasi daging lidah buaya (Aloe vera) dengan metode pemanggangan menggunakan microwave. Jurnal Teknologi Hasil Pertanian, 8(2), 84–98. https://doi.org/10.20961/jthp.v0i0.12896

Safitri, D. N., Sumardianto, S., & Fahmi, A. S. (2019). Pengaruh perbedaan konsentrasi perendaman bahan dalam jeruk nipis terhadap karakteristik kerupuk kulit ikan nila. Jurnal Ilmu dan Teknologi Perikanan, 1(1), 47–54. https://doi.org/10.14710/jitpi.2019.5249

Saisavoey, T., Sangtanoo, P., Reamtong, O., & Karnchanatat, A. (2019). Free radical scavenging and anti-inflammatory potential of a protein hydrolysate derived from salmon bones on RAW 264.7 macrophage cells. Journal of the Science of Food and Agriculture, 99(11), 5112–5121. https://doi.org/10.1002/jsfa.9755

Saliu, F., Magoni, C., Lasagni, M., Della Pergola, R., & Labra, M. (2019). Multi-analytical characterization of perigonadal fat in bluefin tuna: from waste to marine lipid source. Journal of the Science of Food and Agriculture, 99(10), 4571–4579. https://doi.org/10.1002/jsfa.9696

Serradell, A., Montero, D., Fernández-Montero, Á., Terova, G., Makol, A., Valdenegro, V., Acosta, F., Izquierdo, M. S., & Torrecillas, S. (2022). Gill oxidative stress protection through the use of phytogenics and galactomannan oligosaccharides as functional additives in practical diets for european sea bass (Dicentrarchus labrax) juveniles. Animals, 12(23), 1–20. https://doi.org/10.3390/ani12233332

Shamsan, E.F., Al-maqtari, M.A., Noman, M.A. (2019). The effect of un-stable freezing on nutritional value of fish (Indian mackerel – Rastrelliger kanagurta, Russel) from Yemen Coastal Waters. PSM Veterinary Research, 4(2), 40-48.

Shaviklo, G. R., Thorkelsson, G., Rafipour, F., & Sigurgisladottir, S. (2011). Quality and storage stability of extruded puffed corn-fish snacks during 6-month storage at ambient temperature. Journal of the Science of Food and Agriculture, 91(5), 886–893. https://doi.org/10.1002/jsfa.4261

Simionov, I. A., Cristea, V., Petrea, Ş. M., Sîrbu, E. B., Coadă, M. T., & Cristea, D. S. (2016). The presence of heavy metals in fish meat from Danube River: an overview. AACL Bioflux, 9(6), 1388–1399.

Singapurwa, N. M. A. S., Candra, I. P., & Semariyani, A. A. M. (2022). Profil protein ikan lemuru dengan pengeringan oven, pengering matahari dan sinar matahari berbasis SDS Page. Jurnal Teknologi Hasil Pertanian, 15(2), 83–95. https://doi.org/10.20961/jthp.v15i2.53612

Strzyzewska, E., Szarek, J., & Babinska, I. (2016). Morphologic evaluation of the gills as a tool in the diagnostics of pathological conditions in fish and pollution in the aquatic environment: a review. In Veterinarni Medicina, 61(3), 123–132. https://doi.org/10.17221/8763-VETMED

Su, Y., Zhang, M., Bhandari, B., & Zhang, W. (2018). Enhancement of water removing and the quality of fried purple-fleshed sweet potato in the vacuum frying by combined power ultrasound and microwave technology. Ultrasonics Sonochemistry, 44, 368–379. https://doi.org/10.1016/j.ultsonch.2018.02.049

Sweidan, A. H., El-Bendary, N., Hegazy, O. M., Hassanien, A. E., & Snasel, V. (2015). Water pollution detection system based on fish gills as a biomarker. Procedia Computer Science, 65, 601–611. https://doi.org/10.1016/j.procs.2015.09.004

Topic Popovic, N., Kozacinski, L., Strunjak-Perovic, I., Coz-Rakovac, R., Jadan, M., Cvrtila-Fleck, Z., & Barisic, J. (2012). Fatty acid and proximate composition of bluefin tuna (Thunnus thynnus) muscle with regard to plasma lipids. Aquaculture Research, 43(5), 722–729. https://doi.org/10.1111/j.1365-2109.2011.02880.x

Umar, A., Mokolensang, jefrie F., Monijung, R. D., Lumenta, C., Sambali, H., & Sinjal, C. A. L. (2022). Penggunaan limbah ikan tuna sebagai sumber protein untuk pertumbuhan dan kelangsungan hidup ikan nila salin Oreochromis niloticus. Budidaya Perairan, 1(2), 254–262. https://doi.org/https://doi.org/10.35800/bdp.10.2.2022.40576

Uysal, K. (2011). Heavy metal in edible portions (muscle and skin) and other organs (gill, liver and intestine) of selected freshwater fish species. International Journal of Food Properties, 14(2), 280–286. https://doi.org/10.1080/10942910903176378

Wang, D., Xiao, H., Lyu, X., Chen, H., & Wei, F. (2023). Lipid oxidation in food science and nutritional health: a comprehensive review. Oil Crop Science, 8(1), 35–44. https://doi.org/10.1016/j.ocsci.2023.02.002

Wikanta, T., Perwita, R., Sarnianto, P., Murdinah. (2017). Isolasi dan karakterisasi senyawa glikosaminoglikan dari organ ikan pari (Trygon sephen). Jurnal Penelitian Perikanan Indonesia, 8(6), 11-20.

Winarno, F.G. (2008). Kimia Pangan dan Gizi. PT Gramedia Pustaka Utama. Jakarta.

Zhang, X., Zhang, Y., Ding, H., Zhang, W., & Dai, Z. (2022). Effect of washing times on the quality characteristics and protein oxidation of silver carp surimi. Foods, 11(16), 1–14. https://doi.org/10.3390/foods11162397

Published

2025-07-01

How to Cite

Budiadnyani, . I. G. A., Dewi, . R. N. ., Panjaitan, . F. C. A. ., Febrianti, . D. ., Utari, . S. P. S. D. ., Khairunnisa, . A. ., & Nugraha, . I. M. A. . (2025). Pemanfaatan insang ikan tuna (Thunnus sp.) dan lemuru (Sardinella lemuru) sebagai bahan baku pengolahan keripik: Utilization of tuna (Thunnus sp.) and Bali sardinella (Sardinella lemuru) fish gills as raw materials for chips processing. Jurnal Pengolahan Hasil Perikanan Indonesia, 28(6), 530-545. https://doi.org/10.17844/jphpi.v28i6.63268