Photoprotective potential of Eucheuma cottonii extract from Lampung Bay as natural sunscreen agents
Potensi fotoprotektif ekstrak Eucheuma cottonii dari Teluk Lampung sebagai agen tabir surya alami
DOI:
https://doi.org/10.17844/pkg1e410Keywords:
flavonoid, phytochemical, MAAs, seaweed, SPFAbstract
Eucheuma cottonii is a seaweed species that contains bioactive compounds such as flavonoids, hydroquinone phenols, and triterpenoids. However, the presence of mycosporine-like amino acids (MAAs) in E. cottonii from Lampung Bay, Indonesia, remains unexplored, despite their high potential for use in sunscreen creams to enhance the economic value of seaweed. This study aimed to identify MAAs and determine the photoprotective activity of E. cottonii collected from Lampung Bay. MAAs were extracted using hot maceration (40°C) in either 25% methanol or 25% ethanol. Phytochemical analysis, MAAs quantification, and SPF testing were performed to evaluate photoprotective activity. Extraction using hydroethanol yielded a higher rendement than that using hydromethanol. Phytochemical testing confirmed the presence of phenolic and flavonoid compounds in the extracts. MAAs were detected using TLC, but further separation and testing were required to confirm the results. The highest SPF values of 20.18±0.99 (25% ethanol) and 16.48±0.53 (25% methanol extract) fell into the ultra-protection category. These results indicate that E. cottonii from Lampung Bay has a strong potential as a raw material for sunscreen formulation.
References
Al Qoh, S., Amine, J., Assobhei, O., & Etahiri, S. (2024). Investigation of UV protection and anti-aging properties of mycosporine-like amino acids in the Moroccan red alga Gracilaria gracilis (Gracilariales, Rhodophyta). Journal of Applied Phycology, 1-16. https://doi.org/10.1007/s10811-024-03406-8
Alrosyidi, A. F., & Syaifiyatul, H. (2021). Formulasi, evaluasi mutu fisik, dan uji spf krim tabir surya berbahan dasar rumput laut E. cottonii. Majalah Farmasi dan Farmakologi, 25(1), 15-19. https://doi.org/10.20956/mff.v25i1.11967
Álvarez-Gómez, F., Korbee, N., & Figueroa, F. L. (2016). Analysis of antioxidant capacity and bioactive compounds in marine macroalgal and lichenic extracts using different solvents and evaluation methods. Ciencias Marinas, 42(4), 271–288. https://doi.org/10.7773/cm.v42i4.2677
Beladini, S., Susanto, A. B., & Ridlo, A. (2021). Karakteristik krim tabir surya dari Kappaphycus alvarezii Doty 1985 (Florideophyceae : Solieriaceae). Journal of Marine Research, 10(3), 395–402. https://doi.org/10.14710/jmr.v10i3.31272
D’Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. International Journal of Molecular Sciences, 14(6), 12222-12248. https://doi.org/10.3390/ijms140612222
Forestryana, D., & Arnida, A. (2020). Skrining fitokimia dan analisis kromatografi lapis tipis ekstrak etanol daun jeruju (Hydrolea spinosa L.). Jurnal Ilmiah Farmako Bahari, 11(2), 113. https://doi.org/10.52434/jfb.v11i2.859
Ghazipura, M., McGowan, R., Arslan, A., & Hossain, T. (2017). Exposure to benzophenone-3 and reproductive toxicity: a systematic review of human and animal studies. Reproductive Toxicology, 73, 175-183. https://doi.org/10.1016/j.reprotox.2017.08.015
Jofre, J., Celis-Plá, P. S. M., Figueroa, F. L., & Navarro, N. P. (2020). Seasonal variation of mycosporine-like amino acids in three subantarctic red seaweeds. Marine Drugs, 18(2), 75. https://doi.org/10.3390/md18020075
Kageyama, H., & Waditee-Sirisattha, R. (2019). Antioxidative, anti-inflammatory, and anti-aging properties of mycosporine-like amino acids: molecular and cellular mechanisms in the protection of skin-aging. Marine Drugs, 17(4), 222. https://doi.org/10.3390/md17040222
Kasanah, N., Ulfah, M., Imania, O., Hanifah, A. N., & Marjan, M. I. D. (2022). Rhodophyta as potential sources of photoprotectants, antiphotoaging compounds, and hydrogels for cosmeceutical application. Molecules, 27(22). https://doi.org/10.3390/molecules27227788
Kebede, T., Gadisa, E., & Tufa, A. (2021). Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: a possible alternative in the treatment of multidrug-resistant microbes. PloS one, 16(3), e0249253. https://doi.org/10.1371/journal.pone.0249253
Kasitowati, R. D., Wahyudi, A., Asmara, R., Aliviyanti, D., Iranawati, F., Panjaitan, M. A. P., Pratiwi, D. C., & Arsad, S. (2021). Identification photoprotective activity of marine seaweed: Eucheuma sp. IOP Conference Series: Earth and Environmental Science, 679(1). https://doi.org/10.1088/1755-1315/679/1/012014
Kräbs, G., Bischof, K., Hanelt, D., Karsten, U., & Wiencke, C. (2002). Wavelength-dependent induction of UV-absorbing mycosporine-like amino acids in the red alga Chondrus crispus under natural solar radiation. Journal of Experimental Marine Biology and Ecology, 268(1), 69–82. https://doi.org/10.1016/S0022-0981(01)00380-X
Loho, R. E. M., Tiho, M., & Assa, Y. A. (2021). Kandungan dan aktivitas antioksidan pada rumput laut merah. Medical Scope Journal, 3(1), 113–120. https://doi.org/10.35790/msj.v3i1.34986
Maharany, F., Nurjanah, N., Suwandi, R., Anwar, E., & Hidayat, T. (2017). Bioactive compounds of seaweed Padina australis and Eucheuma cottonii as sunscreen raw materials. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(1), 10-17. https://doi.org/10.17844/jphpi.v20i1.16553
Manongko, P. S., Sangi, M. S., & Momuat, L. I. (2020). Uji senyawa fitokimia dan aktivitas antioksidan tanaman patah tulang (Euphorbia tirucalli L.). Jurnal MIPA, 9(2), 64. https://doi.org/10.35799/jmuo.9.2.2020.28725
Messias, M. A., Ferreira, S. M., Tavares, L., & Santos, L. (2023). A comparative study between onion peel extracts, free and complexed with β-cyclodextrin, as a natural UV filter to cosmetic formulations. International Journal of Molecular Sciences, 24(21), 15854. https://doi.org/10.3390/ijms242115854
Miranti, M. R., Anisyah, L., & Hasana, A. R. (2023). Uji identifikasi rhodamin b pada liptint di toko kosmetik kota x menggunakan metode kromatografi lapis tipis. Sains Teknologi Dan Klinis Komunitas, 1(12), 8–13. https://doi.org/10.55681/sentri.v2i11.1765
Mokua, S. K., Mbaria, J. M., Maitho, T. E., & Moriasi, G. A. (2021). Ethnobotanical documentation, phytochemical screening, and cytotoxicity evaluation of medicinal plants used to manage snakebite envenomation in Mwingi West subcounty, Kenya. Evidence‐Based Complementary and Alternative Medicine, 4167296. https://doi.org/10.1155/2021/4167296.
Moldovan, M. L., Iurian, S., Puscas, C., Silaghi-Dumitrescu, R., Hanganu, D., Bogdan, C., Vlase, L., Oniga, I., & Benedec, D. (2019). A design of experiments strategy to enhance the recovery of polyphenolic compounds from Vitis vinifera by-products through heat reflux extraction. Biomolecules, 9(10), 1–18. https://doi.org/10.3390/biom9100529
Moriasi, G. A., Ireri, A. M., & Ngugi, M. P. (2020). In vivo cognitive‐enhancing, ex vivo malondialdehyde‐lowering activities and phytochemical profiles of aqueous and methanolic stem bark extracts of Piliostigma thonningii (Schum.). International Journal of Alzheimer’s Disease, 1367075. https://doi.org/10.1155/2020/1367075.
Morocho‐Jácome, A. L., Freire, T. B., de Oliveira, A. C., de Almeida, T. S., Rosado, C., Velasco, M. V. R., & Baby, A. R. (2021). In vivo SPF from multifunctional sunscreen systems developed with natural compounds—A review. Journal of Cosmetic Dermatology, 20(3), 729-737. https://doi.org/10.1111/jocd.13609
Ngoc, L. T. N., Lee, Y.-C., Moon, J.-Y., Tran, V. V., Chae, M., & Park, D. (2019). Recent trends of sunscreen cosmetic: an update review. Cosmetics, 6(4), 64. https://doi.org/10.3390/cosmetics6040064
Nitulescu, G., Nitulescu, G. M., Lupuliasa, D., & Adam-Dima, I. (2023). Ultraviolet filters for cosmetic applications. Cosmetics, 10(4), 101. https://doi.org/10.3390/cosmetics10040101
Nunes, A. R., Rodrigues, A. L. M., de Queiróz, D. B., Vieira, I. G. P., Neto, J. F. C., Junior, J. T. C., ... & Coutinho, H. D. M. (2018). Photoprotective potential of medicinal plants from Cerrado biome (Brazil) in relation to phenolic content and antioxidant activity. Journal of Photochemistry and Photobiology B: Biology, 189, 119-123. https://doi.org/10.1016/j.jphotobiol.2018.10.013
Nusaibah, Muhammad, T., Pangestika, W., Siregar, A. N., & Utami, K. D. (2023). Karakteristik serum wajah dari sediaan filtrat rumput laut Eucheuma cottonii dan Ulva lactuca. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(3), 545-559. http://dx.doi.org/10.17844/jphpi.v26i3.46874
Olsen, C. M., Loyalka, J., Wilson, L. F., Biswas, N., Green, A. C., & Whiteman, D. C. (2017). Prevention of DNA damage in human skin by topical sunscreens. Photodermatology, Photoimmunology & Photomedicine, 33(3), 135–142. https://doi.org/10.1111/phpp.12298
Ombra, M. N., Palmieri, G., Cossu, A., Paliogiannis, P., Colombino, M., Rozzo, C., Stanganelli, I., Tanda, F., Sini, M. C., & Doneddu, V. (2017). Vitamin D status and risk for malignant cutaneous melanoma: recent advances. European Journal of Cancer Prevention, 26(6), 532–541. https://doi.org/10.1097/cej.0000000000000334
Orfanoudaki, M., Hartmann, A., Ganzera, M., & Karsten, U. (2019). Chemical profiling of mycosporine-like amino acids in twenty-three red algal species. Journal of Phycology, 55(2), 393–403. https://doi.org/10.1111/jpy.12827
Pakki, E., Murdifin, M., Wijoyo, N., & Sumarheni, S. (2018). Study of sunscreen and antioxidant activity of combination extracts from the red algae Eucheuma cottonii and Eucheuma spinosum. Drug Invention Today, 10(9), 1827–1830.
Peng, J., Guo, F., Liu, S., Fang, H., Xu, Z., & Wang, T. (2023). Recent advances and future prospects of mycosporine-like amino acids. Molecules, 28(14), 5588. https://doi.org/10.3390/molecules28145588
Pirotta, G. (2020). Sunscreen regulation in the world (pp. 15–35). Springer. https://doi.org/10.1007/698_2019_440
Pratama, G., Yanuarti, R., Ilhamdy, A. F., & Suhana, M. P. (2019). Formulation of sunscreen cream from Eucheuma cottonii and Kaempferia galanga (zingiberaceae). IOP Conference Series: Earth and Environmental Science, 278(1), 0–7. https://doi.org/10.1088/1755-1315/278/1/012062
Punchakara, A., Prajapat, G., Bairwa, H. K., Jain, S., & Agrawal, A. (2023). Applications of mycosporine-like amino acids beyond photoprotection. Applied and Environmental Microbiology, 89(11), e00740-23. https://doi.org/10.1128/aem.00740-23
Quintero-Rincón, P., Pino-Benítez, N., Galeano, E., Rojo-Uribe, C., Mesa-Arango, A. C., & Flórez-Acosta, O. A. (2023). Sloanea chocoana and S. pittieriana (Elaeocarpaceae): Chemical and biological studies of ethanolic extracts and skincare properties. Plants, 12(23), 3953. https://doi.org/10.3390/plants12233953
Quitério, E., Grosso, C., Ferraz, R., Delerue-Matos, C., & Soares, C. (2022). A critical comparison of the advanced extraction techniques applied to obtain health-promoting compounds from seaweeds. Marine Drugs, 20(11), 677. https://doi.org/10.3390/md20110677
Raal, A., Meos, A., Hinrikus, T., Heinämäki, J., Romāne, E., Gudienė, V., Jakštas, V., Koshovyi, O., Kovaleva, A., Fursenco, C., Chiru, T., & Nguyen, H. T. (2020). Dragendorff’s reagent: Historical perspectives and current status of a versatile reagent introduced over 150 years ago at the University of Dorpat, Tartu, Estonia. Pharmazie, 75(7), 299–306. https://doi.org/10.1691/ph.2020.0438
Ramdani, Y., Ananto, A. D., & Hajrin, W. (2021). Variasi metode ekstraksi dan penetapan nilai SPF ekstrak rumput laut merah (Eucheuma cottonii). Acta Pharmaciae Indonesia : Acta Pharm Indo, 9(1), 31. https://doi.org/10.20884/1.api.2021.9.1.4001
Raymond-Lezman, J. R., & Riskin, S. I. (2024). Sunscreen safety and efficacy for the prevention of cutaneous neoplasm. Cureus, 16(3), e56369. https://doi.org/10.7759/cureus.56369
Reymon, R., Lallo, S., & Manggau, M. A. (2018). Identifikasi senyawa mycosporine-likeamino acids dari fraksi etanol Eucheuma cottonii menggunakan spektrofotometer UV. Majalah Farmasi dan Farmakologi, 22(2), 48–51. https://doi.org/10.20956/mff.v22i2.5700
Rosic, N. N., Braun, C., & Kvaskoff, D. (2015). Extraction and analysis of mycosporine-like amino acids in marine algae. Methods Mol Biol., 1308, 119-29. https://doi.org/10.1007/978-1-4939-2684-8_6.
Roullier, C., Chollet-Krugler, M., Pferschy-Wenzig, E. M., Maillard, A., Rechberger, G. N., Legouin-Gargadennec, B., Bauer, R., & Boustie, J. (2011). Characterization and identification of mycosporines-like compounds in cyanolichens. Isolation of mycosporine hydroxyglutamicol from Nephroma laevigatum Ach. Phytochemistry, 72(11–12), 1348–1357. https://doi.org/10.1016/j.phytochem.2011.04.002
Safia, W., Budiyanti, & Musrif. (2020). Kandungan nutrisi dan senyawa bioaktif rumput laut (Euchema cottonii) dengan metode rakit gantung pada kedalaman berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(2), 261-271. https://doi.org/10.17844/jphpi.v23i2.29460
Sambodo, D. K., & Arlesia, N. (2019). Aktivitas antioksidan krim kombinasi ekstrak Eucheuma cottonii Sumbawa dan ekstrak Citrus lemon L. impor dengan metode DPPH. Health Sciences and Pharmacy Journal, 3(1), 29–33. https://doi.org/10.32504/hspj.v3i1.95
Sami, F. J., Soekamto, N. H., Firdaus, & Latip, J. (2021). Bioactivity profile of three types of seaweed as an antioxidant, uv-protection as sunscreen and their correlation activity. Food Research, 5(1), 441–447. https://doi.org/10.26656/fr.2017.5(1).389
Sari, B. L., Triastinurmiatiningsih, & Haryani, T. S. (2020). Optimasi metode microwave-assisted extraction (MAE) untuk menentukan kadar flavonoid total alga coklat Padina australis. ALCHEMY Jurnal Penelitian Kimia, 16(1), 37–49. https://doi.org/10.20961/alchemy.16.1.34186.38-49
Sari, E. N. (2021). Uji antioksidan dan identifikasi isolat steroid fraksi petroleum eter dan etil asetat hydrilla verticillata hasil kromatografi kolom gradien eluen. [Doctoral dissertation]. Universitas Islam Negeri Maulana Malik Ibrahim.
Sayre, R. M., Agin, P. P., LeVee, G. J., & Marlowe, E. (1979). A comparison of in vivo and in vitro testing of sunscreening formulas. Photochemistry and Photobiology, 29(3), 559-566. https://doi.org/10.1111/j.1751-1097.1979.tb07090.x
Shafie, M. H., Kamal, M. L., Zulkiflee, F. F., Hasan, S., Uyup, N. H., Abdullah, S., Hussin, N.A.M., Tan, Y.C. & Zafarina, Z. (2022). Application of Carrageenan extract from red seaweed (Rhodophyta) in cosmetic products: A review. Journal of the Indian Chemical Society, 99(9), 100613. https://doi.org/10.1016/j.jics.2022.100613
Sinha, R. P., Klisch, M., Gröniger, A., & Häder, D. P. (2000). Mycosporine-like amino acids in the marine red alga Gracilaria cornea—effects of UV and heat. Environmental and Experimental Botany, 43(1), 33-43. https://doi.org/10.1016/S0098-8472(99)00043-X
Sipahutar, Y. H., Albaar, N., Purnamasari, H. B., Kristiany, M. G., & Prabowo, D. H. G. (2019). Seaweed extract (Sargassum polycystum) as a preservative on sunscreen cream with the addition of seaweed porridge. IOP Conference Series: Earth and Environmental Science, 278(1). https://doi.org/10.1088/1755-1315/278/1/012072
Sun, Y., Han, X., Hu, Z., Cheng, T., Tang, Q., Wang, H., Deng, X., & Han, X. (2021). Extraction, isolation and characterization of mycosporine-like amino acids from four species of red macroalgae. Marine Drugs, 19(11), 1–16. https://doi.org/10.3390/md19110615
Tsai, J., & Chien, A. L. (2022). Photoprotection for skin of color. American Journal of Clinical Dermatology, 23(2), 195–205. https://doi.org/10.1007/s40257-021-00670-z
Vega, J., Schneider, G., Moreira, B. R., Herrera, C., Bonomi-Barufi, J., & Figueroa, F. L. (2021). Mycosporine-like amino acids from red macroalgae: UV-photoprotectors with potential cosmeceutical applications. Applied Sciences, 11(11), 5112. https://doi.org/10.3390/app11115112.
Vega, J., Gómez-Pinchetti, J. L., Figueroa, F. L., & Bonomi-Barufi, J. (2020). Cyanobacteria and red macroalgae as potential sources of antioxidants and UV radiation-absorbing compounds for cosmeceutical applications. Marine Drugs, 18(12), 659. https://doi.org/10.3390/md18120659
Volkmann, M., & Gorbushina, A. A. (2006). A broadly applicable method for extraction and characterization of mycosporines and mycosporine-like amino acids of terrestrial, marine and freshwater origin. FEMS Microbiology Letters, 255(2), 286–295. https://doi.org/10.1111/j.1574-6968.2006.00088.x
Wright, C. Y., & Norval, M. (2021). Health risks associated with excessive exposure to aolar ultraviolet radiation among outdoor workers in South Africa: An Overview. Frontiers in Public Health, 9(Suppl 6). https://doi.org/10.3389/fpubh.2021.678680
Yanuarti, R., Nurjanah, N., Anwar, E., & Pratama, G. (2021). Evaluasi fisik sediaan krim tabir surya dari bubur rumput laut Kappaphycus alvarezii dan Turbinaria conoides. Jurnal FishtecH, 10(1), 1–8. https://doi.org/10.36706/fishtech.v10i1.13883
Yuan, S., Huang, J., Jiang, X., Huang, Y., Zhu, X., & Cai, Z. (2022). Environmental fate and toxicity of sunscreen-derived inorganic ultraviolet filters in aquatic environments: A review. Nanomaterials, 12(4), 699. https://doi.org/10.3390/nano12040699.
Zaffina, S., Tucci, M. G., Borra, M., Vinci, M. R., Napolitano, A., Cannatà, V., Camisa, V., & Lembo, M. (2012). Accidental exposure to UV radiation produced by germicidal lamp: case report and risk assessment. Photochemistry and Photobiology, 88(4), 1001–1004. https://doi.org/10.1111/j.1751-1097.2012.01151.x
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