Physicochemical characterization and peptide profiling of gelatin from the invasive species Amphilophus labiatus
Karakteristik fisikokimia dan komposisi peptida gelatin ikan invasif Amphilophus labiatus
DOI:
https://doi.org/10.17844/7ty4zp27Keywords:
collagen , mass spectrometry, peptides, red devilAbstract
Protein- and peptide-based bioactive compounds are a key focus in pharmaceutical and cosmetic development because of their superior biocompatibility, high target specificity, and effectiveness at low dosages. Fish collagen and its derivatives, such as gelatin, are promising candidates as alternative sources of functional biomolecules. Therefore, this study aimed to investigate the potential of red devil fish (Amphilophus labiatus) skin, an abundant invasive species in Indonesia, as a novel source of high-value gelatin. Gelatin was extracted at varying acetic acid concentrations (0, 1, 2, and 3%) and subsequently characterized for its physicochemical properties (gel strength and viscosity) and peptide profile. The results showed that optimal treatment was achieved using 3% acetic acid. A 12.95% gelatin was produced, characterized by a high protein content (87.2%), gel strength of 257.17±17.1 bloom, and viscosity of 6.84±0.87 cP. Advanced characterization using high-resolution tandem mass spectrometry has identified 20 distinct collagen types. The most abundant protein was type I collagen, along with proteins such as collagen IV NC1 and fibrillar collagen NC1. These findings underscore the dual significance of valorizing the skin of the invasive red devil fish, not only as an ecological management strategy but also as a sustainable and innovative source of bioactive proteins for pharmaceutical and cosmetic applications.
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