Ketahanan Beberapa Varietas Padi Lokal terhadap Virus Tungro Isolat Muara
Resistance of Several Local Rice Varieties to Isolate Tungro Virus from Muara
Tungro disease is one of the important diseases in rice because it causes stunted plants and may induce significant yield losses. Planting resistant varieties is a recommended control strategy to overcome tungro disease. The research was conducted to find sources of genetic resistance from local rice varieties that can be used in breeding of tungro disease resistant varieties. The experiment was arranged in a descriptive research design with observational techniques. Observations were made on 16 local rice varieties with each variety consisting of 30 sample plants so that the number of plants used was 480 plants. The isolate of tungro virus collected from rice field in Muara, Bogor was inoculated to the test plants through the insect vector, green leafhopper (Nepothettix virescens). The results showed that the resistance of local varieties varied based on the value of disease index, disease incidence and plant height reduction. ‘TN1’ was the check susceptible variety against tungro virus, while the local rice varieties were differentiated into resistant (‘Utri Merah’), moderate (‘Karau’, ‘Bekongan’, ‘Bujang Bernai’, ‘Srogel Abang’, ‘Pulut Garu’, ‘Randu Range’, ‘Siredep’, ‘Katimpung’, ‘Pulut Sawijan’, ‘Dube’), moderate tend to be susceptible (‘Blumbungan’, ‘Tjere bandung’, ‘Sempor’) and susceptible (‘Rumbai’). All varieties can be infected (disease incidence 27 – 76%), but disease severity varies greatly (3–7). The more severe the disease, the higher the plant height reduction. ‘Srogel Abang’ is a variety that is useful for breeding rice varieties resistant to tungro disease because it showed moderate response with a low percentage of plant height reduction (36%) so that plant productivity can still be maintained.
Abbas S, Sulandari S, Hartono S, Trisnoyo YA. 2020. Molecular detection and resistance respons of six rice varieties to tungro virus from South Sulawesi. JPTI 24(1):89–97. DOI: https://doi.org/10.22146/jpti.47355.
Caguiat XGI, Waing FP, Millas RA, Aquino JD, Tabanao DA. 2020. Genetic diversity of the coat protein gene of Rice tungro bacilliform virus in the Philippines. Philip J Sci. 149(3):511–520.
Choi IR. 2008. Sequiviruses. Encyclopedia of Virology. Ed ke-3. Los Baños (PH): Academic Press. DOI: https://doi.org/10.1016/B978-012374410- 4.00462-3.
Dey SR. 2016. Green leafhopper (GLH), Nephotettix virescens (Distant) and rice tungro disease (RTD). Beats Nat Sci. 3(3–4):1–7.
Galili G. 2011. The aspartate family pathway of plants. Plant Signal Behav. 6(2):192–195. DOI: https://doi.org/10.4161/ psb.6.2.14425.
[INGER] International Network for Genetic Evaluation of Rice. 1996. Standard Evaluation System for Rice. Ed ke-1. Los Baños (PH): Academic Press.
Kumar G, Dasgupta I. 2021. The titer of Rice tungro bacilliform virus dictate the expression level of genes related to cell wall dynamics in rice plant affected by tungro disease. Arch Virol. 166(5):1325– 1336. DOI: https://doi.org/10.1007/ s00705-021-05006-0.
Ladja FT, Widiarta IN. 2012. Varietas unggul baru untuk mengatasi ledakan penyakit tungro. IPTEK Tanaman Pangan 7(1): 18–24.
Lindho JA, Falk BW. 2017. The impact of “coat protein mediated virus resistance” in applied plant pathology and basic research. Phytopatology. 107(6):624–634. DOI: https://doi.org/10.1094/PHYTO-12- 16-0442-RVW.
Liu R, Koyanagi KO, Chen S, Kishima Y. 2012. Evolutionary force of AT-rich repeats to trap genomic and episomal DNAs into the rice genome: lessons from endogenous pararetrovirus. Plant J. 72(5):817–828. DOI: https://doi.org/10.1111/tpj.12002.
Macovei A, Sevilla NR, Cantos C, Gilda B. Jonson GB, Slamet-Loedin I, Cermak T, Voytas DF, Choi IR and Chadha- Mohanty P. 2018. Novel alleles of rice eIF4G generated by CRISPR/Cas9- targeted mutagenesis confer resistance to Rice tungro spherical virus. Plant Biotechnol. J. 16:1918–1927. DOI: https://doi.org/10.1111/pbi.12927.
Muliadi A, Nasrullah, Sumardiyono YB, Trisnoyo YA. 2011. Pewarisan ketahanan penyakit tungro pada galur padi OBST602-28. Penel Pert Tan Pangan. 30(2):121–440.
Nihad SAI, Manidas AC, Hasan K, Hasan MdA, Honey O, Latif MAI. 2021. Genetic variability, heritability, genetic advance and phylogenetic relationship between rice tungro virus resistance and susceptible genotypes revealed by morphological traits and SSR markers. Curr Plant Biol. 25(100194):1–9. DOI: https://doi. org/10.1016/j.cpb.2020.100194.
Rosida N, Komalasari E, Praptana RH. 2020a. Preferensi wereng hijau (Nephotettix virescens Distant.) terhadap galur-galur harapan padi tahan tungro. JIPI. 32(2):105–120. DOI: https://doi.org/10.24246/agric.2020.v32.i2.p105-120.
Rosida N, Kuswinanti T, Amin N, Nasrudin A. 2020b. Epidemiological study on the current status of rice tungro disease in South Sulawesi, Indonesia. J Biol Sci. 20(4):221–231. DOI: https://doi. org/10.3844/ojbsci.2020.221.231.
Senoaji W, Raharjo BT, Tarno H. 2021. Hubungan antara profil protein populasi vektor wereng hijau Nephotettix virescens dan gejala penularan tungro pada tanaman padi. JPPTP. 5(1):25–36. DOI:https://doi.org/10.21082/jpptp. v5n1.2021.p25-36.
Srilatha P, Yousuf F, Methre R, Vishnukiran T, Agarwal S, Poli Y, Raghurami MR, Vidyasagara B, Shanker C, Krishnaveni D, Trivenia S, Brajendra, Praveenc S, Balachandran SM, Subrahmanyam D, Mangrauthia SK. 2019. Physical interaction of RTBV ORFI with D1 protein of Oryza sativa and Fe/Zn homeostasis play a key role in symptoms development during rice tungro disease to facilitate the insect mediated virus transmission. J Virol. 526:117–124. DOI: https://doi. org/10.1016/j.virol.2018.10.012.
Suprihanto, Nurhayati E, Harjosudarmo J. 2013. Virulensi Isolat Rice tungro virus dari beberapa daerah endemis tungro di Indonesia. J Fitopatol Indonesia 9(1):
–37. DOI: https://doi.org/10.14692/jfi.9.1.29.
Suprihanto, Widiarta IN, Kusdiaman D. 2010. Evaluasi virulensi virus tungro dari beberapa daerah endemi dan uji ketahanan plasmanutfah padi. JPTI. 16(1):33–41. DOI: https://doi. org//10.22146/jpti.11749.
Supriyadi, Wijayanti R. 2010. Karakterisasi individu wereng hijau Nephotettix virescens Distant. penular aktif virus tungro padi. JHPT Trop. 10(2):116–467. DOI: https://doi.org/10.23960/j.hptt.210116-122.
Yang H, Ludewig U. 2014. Lysine catabolism, amino acid transport, and systemic acquired resistance. J Plant Signal Behav. 9(e28933):1–4. DOI: https:// doi.org/10.4161/psb.28933.
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