Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island
Abstract
Yellow leaf curl disease in chili pepper has been reported in Bali Island since the early 2012. Research was conducted to identify the virus causing this disease and disease distribution in Bali. Field survey was carried out to observe disease intensity and to collect field samples from several chili pepper growing areas in Bali (Karangasem, Bangli, Tabanan, and Gianyar). Begomovirus identification from field samples was then conducted by polymerase chain reaction method using universal primers SPG1/SPG2, followed by an analysis of the amplified target DNA sequences. The incidence of pepper yellow leaf curl disease reached 100% at all sites and disease severity reached 18%−87%. Begomovirus specific DNA fragment measuring 912 bp was successfully amplified from 12 field samples. Sequence analysis of DNA fragments showed the highest homology with Pepper yellow leaf curl Indonesia virus (PYLCIV). Further phylogenetic analysis confirmed the relationship between PYLCIV isolates from Bali and various PYLCIV isolates from Indonesia.
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References
Annisaa NW, Hidayat P, Giyanto, Hidayat SH, Lee S. 2021. Multiple infections of Begomovirus on its host plants. IOP Conf Ser: Earth Environ Sci. 694:1–8. DOI: https://doi.org/10.1088/1755-1315/694/1/
[BPS] Badan Pusat Statistik Provinsi Bali. 2015. Produksi Cabai Provinsi Bali Menurut Kabupaten/Kota 2011–2015. https://bali.bps.go.id/dynamictable/2017/05/18/158/produksi-cabe-provinsi-bali-menurut-kabupaten-kota-2000-2016.html. Cited 28 Oct 2020.
Doyle JJ, Doyle JJ. 1987. A rapid DNA isolation of procedure for small quantities of fresh leaf tissue. Phytocheml bull. 19:11–19.
Ganefianti DW, Sujiprihati S, Hidayat SH, Syukur M. 2008. Metode penularan dan uji ketahanan genotipe cabai (Capsicum spp.) terhadap Begomovirus. J Akta Agros. 11(2):162–169.
Lee H, Park J, Auh CK, Lee KY, Kim CS, Lee GS, Soh HC, Choi HS, Lee SC. 2011. Molecular evidence of recombination on Korea isolates of Tomato yellow leaf curl virus by nucleotide transversions and transsitions. Plant Pathol J. 27(4):378–384. DOI: https://doi.org/10.5423/PPJ.2011.27.
378.
Li R, Salih S, Hurtt S. 2004. Detection of Begomoviruses in sweetpotato by polymerase chain reaction. Plant Dis. 88(12):1347–1351. DOI: https://doi.org/10.
/PDIS.2004.88.12.1347.
Putra IGNBP, Puspawati NM, Nyana IDN, Siadi IK, Suastika G. 2015. Identifikasi virus yang berasosiasi dengan penyakit mosaik, kuning, dan klorosis pada tanaman cabai rawit (Capsicum frutescens L.). JAT. 4(3):244–252.
Rojas MR, Gilbertson RL, Russel DR, Maxwell DP. 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted Geminiviruses. Plant Dis. 77:340–347. DOI: https://doi.org/10.1094/PD-77-0340.
Sakata J, Shibuya Y, Sharma P, Ikegami M. 2008. Strains of a new bipartite Begomovirus, Pepper yellow leaf curl Indonesia virus, in leaf-curl-diseased tomato and yellow-vein-diseased ageratum in Indonesia. Arch Virol. 153(12):2307–2313. DOI: https://doi.org/10.1007/s00705-008-0254-z.
Santoso TJ, Hidayat SH, Herman M, Aswidinnoor H, Sudarsono. 2008. Identitas dan keragaman genetik Begomovirus yang berasosiasi dengan penyakit kuning pada tomat berdasarkan teknik polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). J Agrobiogen. 4(1):9–17. DOI: https://doi.org/10.21082/jbio.v4n1.2008.p9-17.
Selangga DGW, Hidayat SH, Susila AD, Wiyono S. 2019. The effect of silica (SiO2) to the severity of Yellow leaf curl disease on chili pepper. JPTI. 23(1):54–60. DOI: https://doi.org/10.22146/jpti.38951.
Selangga DGW, Listihani. 2021. Molecular identification of Pepper yellow leaf curl Indonesia virus on chili pepper in Nusa Penida Island. J HPT Trop. 21(2):97–102. DOI: https://doi.org/10.23960/jhptt.22197-
Setiawati W, Udiarto BK, Soetiarso TA. 2008. Pengaruh varietas dan sistem tanam cabai merah terhadap penekanan populasi hama kutu kebul. J Hort. 18(1):55–61.
Soetiarso TA, Setiawati W. 2010. Kajian teknis dan ekonomis sistem tanam dua varietas cabai merah di dataran tinggi. J Hort. 20(3):284–298.
Sudiono, Hidayat SH, Hidayat P. 2005. The distribution and molecular detection of geminivirus pathogen of chilli yellowing disease in Sumatera lsland. J HPT Trop. 5(2):113–121. DOI: https://doi.org/10.23960/
j.hptt.25113-121.
Sulandari S, Susesno R, Hidayat SH, Harjosudarmo J, Sosromarsono S. 2006. Deteksi dan kajian kisaran inang virus penyebab penyakit daun keriting kuning cabai. Hayati. 13(1):1–6. DOI: https://doi.org/10.1016/S1978-3019(16)30371-0.
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