Antibacterial activity and peptides characterization of partial-purified neutralized cell-free supernatant of bacterial isolates from pado fermented fish
Aktivitas antibakteri dan karakterisasi peptida supernatan murni parsial dari isolat bakteri asal ikan fermentasi pado
DOI:
https://doi.org/10.17844/jphpi.v28i7.63298Keywords:
antibacterial peptide, fermented fish, Lactiplantibacillus plantarum, specific activity, ultrafiltrationAbstract
Pado is a traditional fermented food from West Sumatra, made by combining pelagic fish with grated coconut and dried seeds of Pangium edule. Lactic acid bacteria are the predominant microorganisms present and generally produce metabolites that contribute to prolonging the shelf life of the product. The neutralized cell-free supernatant (NCFS) of these isolates may contain bioactive peptides, such as antibacterial peptides. This study aimed to obtain partially purified NCFS from pado isolates with the best inhibitory activity against Salmonella typhimurium and Staphylococcus aureus using agar diffusion methods. Crude NCFS was obtained from seven isolates from pado by centrifuging their growth media, and the supernatant was neutralized. The proteinaceous nature of crude NCFS was confirmed after artificial digestion with proteolytic pepsin. Ultrafiltration was used to partially purify crude NCFS. Antibacterial peptides were further analyzed using liquid chromatography-high resolution mass spectrometry (LC-HRMS). All NCFS showed antibacterial activity against S. typhimurium, but only three isolates showed apparent inhibitory activity against S. aureus. The Lactiplantibacillus plantarum B3Iw4 isolate was selected for further study. The low-molecular-weight NCFS fraction (in the range of 317.20–1,494.70688 Da) after ultrafiltration by a 3 kDa membrane exhibited a threefold increase in specific activity against S. aureus compared to the crude NCFS. LC-HRMS analysis identified 20 peptides in the partially purified NCFS. The peptide, AAERAGAAALAMHGR, was predicted to have antibacterial activity after matching with the BIOPEP-UWM database, with a matched sequence AA. This peptide has relatively high hydrophobicity and is interesting for further study.
References
Afrin, S., Hoque, M.A., Sarker, A.K., Satter, M.A., & Bhuiyan, M.N.I. (2021). Characterization and profiling of bacteriocin-like substances produced by lactic acid bacteria from cheese samples. Access Microbiology, 3, 1–9. https://doi.org/10.1099/acmi.0.000234
Ageni, L., Ajibade, G., Yerima, B., & Appah, J. (2017). Shelf life extension study of ogi and fufu using bacteriocin isolated from Lactobacillus acidophilus of fermented dairy products. African Journal of Microbiology Research, 11(32), 1286–1293. https://doi.org/10.5897/ajmr2017.8620
Al-Hammam, M. Y., Putra, M. M. P., Mardinsyah, A. H., Cahyati, G., & Puspita, I. D. (2023). Antibacterial activities of Lactobacillus sp. GMP1 and Weisella sp. GMP12 against some foodborne disease causing-bacteria. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(2), 206–215. https://doi.org/10.17844/jphpi.v26i2.44618
Balouiri, M., Sadiki, M., & Ibnsouda, A. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. https://doi.org/10.1016/j.jpha.2015.11.005
Cao, F., Ma, G., Song, M., Zhu, G., Mei, L., & Qin, Q. (2021). Evaluating the effects of hydrophobic and cationic residues on antimicrobial peptide self-assembly. Soft Matter, 17(16), 4445–4451. https://doi.org/10.1039/d1sm00096a
Cleveland, J., Montville, T.J., Nes, I.F., & Chikindas, M.L. (2001). Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology, 71, 1-20.
[CLSI] Clinical and Laboratory Standards Institute. (2012). Performance standards for antimicrobial disk susceptibility tests: approved standard - eleventh edition. Clinical and Laboratory Standards Institute.
Golneshin, A., Gor, M. C., Williamson, N., Vezina, B., Van, T. T. H., May, B. K., & Smith, A. T. (2020). Discovery and characterisation of circular bacteriocin plantacyclin B21AG from Lactiplantibacillus plantarum B21. Heliyon, 6(2020), 1-14. https://doi.org/10.1016/j.heliyon.2020.e04715
Hasbullah, Kasim, A., Novelina, & Nurdin, H. (2016b). Characterization traditional food pado from West Sumatra. Der Pharmacia Lettre, 8(15), 26-31.
Hasbullah, Kasim A., Novelina, & Nurdin H. (2016a). Existence of pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Vibrio parahaemolyticus) in pado traditional food from West Sumatera. Der Pharm Lett, 8(15), 202–205.
Hassan, M. U., Nayab, H., Rehman, T. U., Williamson, M. P., Haq, K. U., Shafi, N., Shafique, F., & De Guía Córdoba, M. (2020). Characterisation of bacteriocins produced by Lactobacillus spp. isolated from the traditional Pakistani yoghurt and their antimicrobial activity against common foodborne pathogens. BioMed Research International, 2020, 1-10. https://doi.org/10.1155/2020/8281623
Heredia-Castro, P. Y., Méndez-Romero, J. I., Hernández-Mendoza, A., Acedo-Félix, E., González-Córdova, A. F., & Vallejo-Cordoba, B. (2015). Antimicrobial activity and partial characterization of bacteriocin-like inhibitory substances produced by Lactobacillus spp. isolated from artisanal Mexican cheese. Journal of Dairy Science, 98(12), 8285–8293. https://doi.org/10.3168/jds.2015-10104
Hu, M., Dang, L., Zhao, H., Zhang, C., Lu, Y., Yu, J., & Lu, Z. (2016). Characterization and antibacterial mode of a novel bacteriocin with seven amino acids from Lactobacillus plantarum in Guizhou salted radish. Journal of Agricultural Science, 8(10), 120-130. https://doi.org/10.5539/jas.v8n10p120
Ibrahim, F., Siddiqui, N. N., Aman, A., Qader, S. A. U., & Ansari, A. (2019). Characterization, cytotoxic analysis and action mechanism of antilisterial bacteriocin produced by Lactobacillus plantarum isolated from cheddar cheese. International Journal of Peptide Research and Therapeutics, 26(4), 1751–1764. https://doi.org/10.1007/s10989-019-09982-5
Kamal, I., Ashfaq, U.A., Hayat, S., Aslam, B., Hassan, S., Yaseen, H., Rajoka, M.S.R., Shah, A.A., & Khurshid, M. (2023). Prospects of antimicrobial peptides as an alternative to chemical preservatives for food safety. Biotechnology Letters, 45, 137–162. https://doi.org/10.1007/s10529-022-03328-w
Klaenhammer, T. R. (1988). Bacteriocins of lactic acid bacteria. Biochimie, 70(3), 337–349. https://doi.org/10.1016/0300-9084(88)90206-4
Kumar, V., Sheoran, P., Gupta, A., Yadav, J. P., & Tiwari, S. K. (2016). Antibacterial property of bacteriocin produced by Lactobacillus plantarum LD4 isolated from a fermented food. Annals of Microbiology, 66(4), 1431–1440. https://doi.org/10.1007/s13213-016-1230-6
Kurnianto, M. A., Kusumaningrum, H. D., Lioe, H. N., & Chasanah, E. (2021). Partial purification and characterization of bacteriocin-like inhibitory substances produced by Streptomyces sp. isolated from the gut of Chanos chanos. BioMed Research International, 2021, 1–12. https://doi.org/10.1155/2021/7190152
Kurnianto, M. A., Lioe, H. N., Chasanah, E., & Kusumaningrum, H. D. (2022). Purification, HR-LC-ESI-MS-MS identification, and peptide prediction of bacteriocin-like inhibitory substances produced by Streptomyces sp. isolated from Chanos chanos. International Journal of Food Science, 2022, 1-14. https://doi.org/10.1155/2022/8672643
Liu, C. J., Luo, M. Y., Li, Q. K., Deng, G., Li, X. R., Yang, E., & Luo, Y. Y. (2019). Analysis of the antimicrobial activity of: Lactobacillus plantarum YM-4-3: implications of suitable conditions for extending the shelf life of fermented soybean products. Food and Function, 10(9), 5282–5289. https://doi.org/10.1039/c9fo00672a
Liu, J. M., Fehér, C., Cao, M., Lu, F., & Jensen, P. R. (2021). Editorial: lactic acid bacteria: microbial metabolism and expanding applications. Frontiers in Bioengineering and Biotechnology, 9(794164), 1–4. https://doi.org/10.3389/fbioe.2021.794164
Morales, G., Sierra, P., Mancilla, A., Paredes, A., Loyola, L. A., Gallardo, O., & Borquez, J. (2003). Secondary metabolites from four medicinal plants from Northern Chile: antimicrobial activity and biotoxicity against Artemia salina. Journal of the Chilean Chemical Society, 49(1), 44–49. https://doi.org/10.4067/s0717-97072003000200002
Nguyen, V. D., Pham, T. T., & Pham, N. M. Q. (2014). Two novel strains of bacteriocin-producing Lactobacillus plantarum and their application as biopreservative in chill-stored fresh cobia meat. Journal of Pure and Applied Microbiology, 8(2), 1547-1557.
Nofiani, R., Elminah, E., & Ardiningsih, P. (2019). Chemical and microbiological properties of buduk, a commercial fish sauce from West Kalimantan. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 601–608. https://doi.org/10.17844/jphpi.v22i3.29230
Pei, J., Jin, W., Wang, J., Huang, Y., Li, X., Zhang, H., Zhang, Y., Ramadan, A., & Abd El-Aty, A. M. (2021). Purification and characterization of plantaricin ykx and assessment of its inhibitory activity against Alicyclobacillus spp. Frontiers in Microbiology, 12(783266), 1–11. https://doi.org/10.3389/fmicb.2021.783266
Sari, N. P., Sari, R., & Untari, E. K. (2018). Antibacterial activity test of bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum against Gram positive pathogenic bacteria. Journal of Tropical Biodiversity and Biotechnology, 3(2018), 85-91. https://doi.org/10.22146/jtbb.38138
Silva, C. C. G., Silva, S. P. M., & Ribeiro, S. C. (2018). Application of bacteriocins and protective cultures in dairy food preservation. Frontiers in Microbiology, 9(594), 1-15. https://doi.org/10.3389/fmicb.2018.00594
Syafitri, Y., Kusumaningrum, H. D., & Dewanti-Hariyadi, R. (2022). Identification of microflora and lactic acid bacteria in pado, a fermented fish product prepared with dried Pangium edule seed and grated coconut. Food Science and Technology, 42, 1–9. https://doi.org/10.1590/FST.19921
Wang, Y., Qin, Y., Xie, Q., Zhang, Y., Hu, J., & Li, P. (2018). Purification and characterization of plantaricin LPL-1, a novel class IIa bacteriocin produced by Lactobacillus plantarum LPL-1 isolated from fermented fish. Frontiers in Microbiology, 9, 1–12. https://doi.org/10.3389/fmicb.2018.02276
Zhang, J., Liu, G., Shang, N., Cheng, W., Chen, S., & Li, P. (2009). Purification and partial amino acid sequence of pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31-1. Journal of Food Protection, 72(12), 2524–2529. https://doi.org/10.4315/0362-028X-72.12.2524
Zhang, Y. M., Yang, L. Y., Ying, J. P., Fu, C. M., Wu, G., Li, X. R., & Zhang, Q. L. (2023). A novel bacteriocin RSQ01 with antibacterial activity and its application and metabolomic mechanism in milk preservation. Food Control, 151, 1-11. https://doi.org/10.1016/j.foodcont.2023.109823
Zhou, F., Zhao, H., Bai, F., Dziugan, P., Liu, Y., & Zhang, B. (2014). Purification and characterisation of the bacteriocin produced by Lactobacillus plantarum, isolated from Chinese pickle. Czech Journal of Food Sciences, 32(5), 430–436. https://doi.org/10.17221/270/2013-cjfs
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