Morfologi Mutan Alfalfa (Medicago sativa L.) Hasil Iradiasi Sinar Gamma Pada Cekaman Kering
Mutant Morphology of Alfalfa (Medicago sativa L) Result of Gamma Ray Irradiation in Dry Stress
Abstract
Alfalfa in dry stress conditions cannot grow optimally. Drought inhibits growth and reduces alfalfa production in many cultivation systems. Plants breeding with gamma-ray irradiation and in vitro selection using PEG found somaclonal variants adapted to grow well in drought conditions. This study aims to determine the type of mutation with the best morphology in dry stress conditions. Alfalfa mutants resulting from gamma irradiation were sub-cultured in vitro. Alfalfa mutants cultured in PEG medium, according to treatments. This experiment used completely randomized factorial design with 2 factors, namely the gamma-ray irradiation doses (0 Gy, 100 Gy, 200 Gy, 300 Gy, 400 and 500 Gy), and PEG concentrations (5%, 10%, 15%, and 20% ). The variables observed were viability, plant height, number of leaves, wet weight, medium shrinkage, leaf wilting, and leaf color. The results showed that the gamma-irradiated mutant had a better (p<0.05) morphology than the control (0 Gy) in the face of dry stress conditions. Combination of 500 Gy and 20% PEG concentration resulted the best type of mutant.
Key words: alfalfa, in vitro, gamma rays, PEG
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References
Anower MR, Boe A, Auger D, Mott IW, Peel MD, Xu L, Kanchupati P &Wu Y. 2015. Comparative drought response in eleven diverse alfalfa accessions. Journal of Agronomy and Crop Science. 203(1):1-13.
Anjum SA, Xie XY, Wang LC, Saleem MF, Man C & Lei W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research. 6(9.):2026-2032.
Aristya VE &Taryono. 2019. Pemuliaan tanaman partisipatif untuk meningkatkan peran varietas padi unggul dalam mendukung swasembada pangan nasional. Agrotechnology Innovation . 2(1):026-035.
Astutik A. 2009. Peningkatan kualitas bibit pisang kepok melalui radiasi sinar gamma secara in vitro. Buana Sains. 9(1):69-75.
Chahal GS & Gosal SS. 2002. Principle and Procedures of Plant Breeding: Biotechnology and Conventional Approaches, UK : Alpha Science Internasional
Dwiyani R. 2015. Kultur Jaringan Tanaman. Denpasar Barat (ID): Pelawan Sari “Percetakan & Penerbit”
Harsanti L & Yulidar Y. 2015. Pengaruh irradiasi sinar gamma terhadap pertumbuhan awal tanaman kedelai (Glycine max (L.) Merill) varietas denna 1. Prosiding Pertemuan dan Presentasi Ilmiah ‐ Penelitian Dasar Ilmu Pengetahuan dan Teknologi Nuklir, Yogyakarta (ID): Pusat Sains dan Teknologi Akselerator (PSTA)
Badan Tenaga Nuklir Nasional
Hemon F. 2009. Induksi mutasi dengan iradiasi sinar gamma dan seleksi in vitro untuk mendapatkan embrio somatik kacang tanah yang toleran polietilena glikol. Jurnal Agrotropika.14(2):67–72.
Havidzati N, Karti PDMH & Prihantoro I. 2017. Morphology response of alfalfa (Medicago sativa L.) based on level gamma ray irradiation with tissue culture methods. The 7TH Annual Basic Science International Conference, Malang(ID) : Faculty of Science, Universitas Brawijaya
Hutami S. 2008. Masalah pencoklatan pada kultur jaringan. Jurnal Agrobiogen. 4(3):83-88.
Jardine KJ, Chambers JQ, Holm J, Jardine AB, Fontes CG, Zorzanelli RF & Manzi AO. 2015. Green leaf volatile emissions during high temperature and drought stress in a central amazon rainforest. Plants. 4(3):678-690.
Liu X, Fan Y, Long J, Wei R, Kjelgren R, Gong C & Zhao J. 2013. Effects of soil water and nitrogen availability on photosynthesis and water use efficiency of Robinia pseudoacacia seedlings. Journal of Environmental Sciences. 25(3):585-595.
Maslukah R, Yulianti F, Roviq M & Maghfoer MD. 2019. Pengaruh polyethylene glycol (peg) terhadap hardening planlet apel (Malus sp.) akibat hiperhidrisitas secara in vitro. Jurnal of Agricultural Science. 4(1):30-38.
Meliala JHS, Basuki N & Soegianto A. 2016. Pengaruh iradiasi sinar gamma terhadap perubahan fenotipik tanaman padi gogo (Oryza sativa L.). Jurnal Produksi Tanaman. 4(7):585-594.
Putnam DH & Orloff SB. 2014. Forage Crops in Encyclopedia of Agriculture and Food Systems. volume 3. California (USA) : Elsevier Inc.
Radovic J, Sokolovic D & Markovic J. 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotecnology in Animal Husbandry. 8(5):321-336.
Rosawanti P. 2016. Pertumbuhan akar kedelai pada cekaman kekeringan. Jurnal Ilmiah Pertanian dan Kehutanan. 3(1):21-28.
Sajimin. 2011. Medicago sativa L (alfalfa) sebagai tanaman pakan ternak harapan di Indonesia. Wartazoa. 2(21):91-98.
Saking N & Qomariyah N. 2017. Identifikasi hijauan makanan ternak (hmt) lokal mendukung produktivitas sapi potong di sulawesi selatan. Seminar Nasional Teknologi Peternakan dan Veteriner Bogor (ID): Badan Libang Pertanian
Saragih SHY, K Rizal & Sitanggang KD. 2020. Induksi mutasi kara benguk (Mucuna pruriens L.) menggunakan iradiasi sinar gamma. Agrosains. 22(2):105-108.
Subantoro R. 2009. Mengenal karakter mutan alfalfa (Medicago sativa L.). Mediumgro. 5(2):50–62.
Sujinah A & Jamil. 2016. Mekanisme respon tanaman padi terhadap cekaman kekeringan dan varietas toleran. Iptek Tanaman Pangan. 11(1):1-8.
Sutjahjo SH, Abdul K & Ika M. 2007. Efektivitas polietilena glikol sebagai bahan penyeleksi kalus nilam yang diiradiasi sinar gamma untuk toleransi terhadap cekaman kekeringan. Jurnal Ilmu-ilmu Pertanian Indonesia. 9(1):48-57.
Ulfa M, Zulkarnain & Rainiyati. 2018. Pengaruh pemberian poli etilen glikol (peg) konsentrasi subletal pada perkembangan kalus kedelai (Glycine Max L. Merill) secara in vitro. [Artikel] Jambi (ID) : Universitas Jambi. (http://repository.unja.ac.id/id/eprint/3278).
Wardhani MUD, Puspitaningtyas DM, Dinarti D. 2007. Pengaruh iradiasi sinar gamma terhadap pertumbuhan anggrek brachypeza indusiata (Reichb. f) secara in vtro. Buletin Kebun Raya Indonesia. 10(2):53-59.
Wardhani PCK, Suryawati A & Lagiman. 2018. Uji kekeringan terhadap viabilitas dan vigor benih beberapa varietas cabai (Capsicum Annum L.) dengan berbagai konsentrasi peg-6000 di fase perkecambahan dan pertumbuhan. Agrivet. 24(1):1-11
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