Tingkat Toleransi Tanaman Lamtoro mini (Desmanthus virgatus) terhadap Cekaman Salinitas melalui Teknik Kultur Jaringan
Tolerance Level of Desmanthus virgatus to Salinity Stress through Tissue Culture Techniques
Abstract
Desmanthus virgatus has high potential as a source of animal feed and has the opportunity to develop as forage crops in saline areas. This study aimed to obtain information on the tolerance level of D.virgatus to salinity stress at different levels using tissue culture techniques. The experimental design used was a completely randomized design (CRD) with 5 treatments and 25 replications. The treatments were P0 (NaCl 0 ppm), P1 (NaCl 2500 ppm), P2 (NaCl 5000 ppm), P3 (NaCl 7500 ppm), and P4 (NaCl 10000 ppm). The variables observed were plant height, number of compound leaves, number of leaf twigs, leaf color, leaf loss, and plant viability. The results showed that the addition of NaCl with a level of more than 2500 ppm had a significant (p<0.05) effect on plant height, number of compound leaves, number of leaf twigs, and leaf color, but had no significant effect on leaf loss and plant viability. It is concluded D.virgatus had a tolerance level of salinity stress up to 2500 ppm.
Downloads
References
Absari EU & Kuswanto. 2019. Respon beberapa genotip kacang tunggak (Vigna unguiculate L.) terhadap cekaman salinitas. Journal of Agricultural Science. 4(1):57-67.
Basyuni, Lollie APP, Berliana N & Putri E. 2014. Growth and biomass in response to salinity and subsequent fresh water in mangrove seedlings Avicennia marina and Rhizophora stylosa. Journal of Tropical Forest Science. 20(1):17-25.
Dianawati M, Handayani DP, Matana YR & Belo SM. 2013. Pengaruh cekaman salinitas terhadap viabilitas dan vigor benih dua varietas kedelai (Glycine max. L.). Journal of Agriculture Science. 3(2):35-41.
Duan D, Liu X, Khan MA & Gul B. 2004. Effect of salt and water stress on the germination of Chenopodium Glaucum L. seed. Pakistan Journal of Botany. 36 (4): 793-800.
Ferguson J. 2012. Color Name Diagrams for the Munsell Color Charts for Plant Tissues. Toronto (CA) : Univ. Toronto Canada.
Fuskhah E, Soetrisno RD, Anwar S & Kusmiyati F. 2014. Kajian morfologi dan fisiologi ketahanan leguminosa pakan. Agromedia. 32(2): 45-53.
Hamayun M, Khan SA, Khan AL, Shinwari ZK, Hussain J, Sohn E, Kang SM, Kim YH, Khan MA & Lee IJ. 2010. Effect of salt stress on growth attributes and endogenous growth hormones of soybean cultivar. Pakistan Journal of Botany. 42(5):3103–3112.
Hidayat Y. 2008. Keefektifan bahan sterilisasi dalam pengendalian kontaminasi pada pertumbuhan kultur Zygotik surian (toona sinensis roem). Wanamukti. 6 (1): 35-44.
Lazier JR & Ahmad N. 2016. Tropical Forage Legumes: Harnessing The Potential of Desmanthus and Other Genera For Heavy Clay Soils. Wallingford (UK): CAB International.
MacEvoy B. 2005. Modern Color Models – Munsell Color System. Color Vision.
Mahata ME, Rizal Y & Nuraini. 2010. Pengolahan daun Lamtoro mini (Desmanthus virgatus) dengan tekanan uap panas sebagai pakan alternatif sumber protein nabati untuk ternak unggas. Journal of Animal Science. 3(2):32-38.
Muharam M & Saefudin A. 2016. Pengaruh berbagai pembenah tanah terhadap pertumbuhan dan populasi tanaman padi sawah (Oryza sativa, L) varietas dendang di tanah salin sawah bukaan baru. Indonesian Journal of Agrotech. 1(2):141- 150.
Nugroho WS. 2015. Penetapan standar warna daun sebagai upaya identifikasi status hara (N) tanaman Jagung (Zea mays L.) pada tanah regosol. Journal of Agriculture Science. 3(1):8-15.
Rachman A, Subiksa IGM & Wahyunto. 2007. Perluasan Areal Tanaman Kedelai ke Lahan Suboptimal. Jakarta (ID): Puslitbangtan.
Romadloni A & Wicaksono KP.2018. Pengaruh beberapa level salinitas terhadap perkecambahan kacang hijau (Vigna radiata L) varietas Vima. Jurnal Produksi Tanaman 6 (8) : 1663-1670
Sadjad S. 2004. Kuantifikasi Metabolisme Benih. Jakarta (ID): PT Grasindo.
Sposito G. 2008. The Chemistry of Soils. New York (NY): Oxford University Press.
Steel RGD & Torrie JH. 1989. Principle and Procedure of Statistics. New York (USA): McGraw Hill Book Co. Inc.
Suswati, Sumarsono, Kusmiyati F. 2012. Pertumbuhan dan produksi rumput benggala (Panicum maximum) pada berbagai upaya perbaikan tanah salin. Journal of Agricultural Science. 1(1): 297-306.
Tuteja N. 2007. Mechanisms of high salinity tolerance in plants, chapter twentyfour. Methods Enzymol. 428(2):419-438.
Wattimena GA, Wiendi NM, Ansori N, Purwito A, Efendi D, Khumaida N & Purwoko BS. 2011. Bioteknologi dalam Pemuliaan Tanaman. Bogor (ID): IPB Press.
Copyright (c) 2022 C H Nufus, I Prihantoro, P D M H Karti
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors of the submitted manuscript have to understand and agree that the copyrights published are held by Jurnal Ilmu Nutrisi dan Teknologi Pakan. Copyrights includes rights in reproducing, distributing and selling every section of articles in all forms and media. The copyright transfer form is signed by the corresponding author. The author”
• Creative Commons Attribution (CC BY)
you are allowed to:
Share – copy and redistribute the material in any medium or format
Adapt – remix, transform, and build upon the material
for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal)
This work is licensed under a Creative Commons Attribution 4.0 International License.