FIRST CONFIRMED RECORD OF THE NEAR-THREATENED BOMBAY DUCK (Harpadon nehereus) IN CILACAP WATERS, INDONESIA: REKAMAN TERKONFIRMASI PERTAMA IKAN KADAL (Harpadon nehereus) YANG HAMPIR TERANCAM PUNAH DI PERAIRAN CILACAP, INDONESIA

Sugeng Hartono; Novia Nurul  Afiyah; Mustathi'  Zulfa; Muhammad Browijoyo  Santanumurti; Muyassar Hamid  Abualreesh; Gondo Puspito; Muhammad Natsir  Kholis

doi:10.24319/jtpk.17.278-290

Authors

Sugeng Hartono Study Program of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto Utara, Banyumas 53122, Indonesia https://orcid.org/0000-0002-2317-7648
Novia Nurul Afiyah Study Program of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto Utara, Banyumas 53122, Indonesia https://orcid.org/0009-0001-3638-8639
Mustathi' Zulfa Study Program of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto Utara, Banyumas 53122, Indonesia
Muhammad Browijoyo Santanumurti Department of Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Jl. Mulyorejo, Surabaya 60115, Indonesia https://orcid.org/0000-0003-0846-9447
Muyassar Hamid Abualreesh 1) Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Al Oloum St., Jeddah 22254, Saudi Arabia; 2) Center of Excellence for Environmental Studies (CEES), King Abdulaziz University, Idara St., Jeddah 22254, Saudi Arabia https://orcid.org/0000-0003-3170-3609
Gondo Puspito Department of Fisheries Resource Utilization, Faculty of Fisheries and Marine Sciences, IPB University, Jl. Agatis, IPB Dramaga Campus, Bogor 16680, Indonesia https://orcid.org/0000-0002-1573-7334
Muhammad Natsir Kholis Department of Fisheries Resources Utilization, Faculty of Fisheries and Marine Sciences, Riau University, Jl. Muchtar Lutfi, Pekanbaru 28293, Indonesia https://orcid.org/0000-0001-9172-4490

DOI:

https://doi.org/10.24319/jtpk.17.278-290

Keywords:

Bombay duck, DNA barcoding, fisheries management, species identification

Abstract

Accurate species identification is an essential foundation for effective fisheries management and conservation in Indonesia, particularly for near‑threatened species. This study aims to identify Bombay duck (Harpadon nehereus) from Cilacap Waters through morphological and molecular analyses, document its first record in Cilacap Waters, and provide a scientific basis for population studies, fisheries management, and conservation. Morphological identification was performed using physical characteristics such as shape, colour, and general features for morphometric and meristic analyses, guided by identification keys. Molecular confirmation was carried out through DNA barcoding of the mitochondrial COI gene. Primer Fish F1 and Fish R1 were used, along with the Sanger dideoxy sequencing method. The subfamily was confirmed by the presence of inner rays of the pelvic fin that are subequal or slightly longer than the outer rays. The genus was identified by the lateral‑line scales being enlarged and extending as a median lobe of the caudal fin. The primary rays of the caudal fin were without scales, and the body was compressed. The species was identified as H. nehereus because the pectoral fins reach beyond the origin of the dorsal fin. The obtained sequence (accession PV688437.1) showed approximately 96.928–99.495% identity with conspecific references in global databases and formed the same clade with other H. nehereus sequences in phylogenetic analysis, confirming its genetic identity. As the first confirmed record of H. nehereus in Cilacap, these findings provide essential baseline data for stock assessment, fisheries management, and conservation efforts in Indonesian waters.

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