THE EFFECT OF HIGH-PROTEIN FEEDING FREQUENCY OF UFBs-RAS ON THE PRODUCTION PERFORMANCE OF GOURAMI (Osphronemus goramy) FRY

PENGARUH FREKUENSI PEMBERIAN PAKAN PROTEIN TINGGI PADA UFBs-RAS TERHADAP PERFORMA PRODUKSI BENIH GURAMI (Osphronemus goramy)

Authors

  • Ujang Subhan 1) Fisheries Study Program, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia; 2) Institutional College Center of Excellence for Functional Nanopowder, Padjadjaran University, Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia
  • Nur Fatimatuz Zahra Fisheries Study Program, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia
  • Atikah Nurhayati Fisheries Study Program, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia
  • Irfan Zidni Fisheries Study Program, Faculty of Fisheries and Marine Sciences, Padjadjaran University, Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363, Indonesia

DOI:

https://doi.org/10.24319/jtpk.17.174-185

Keywords:

feeding frequency, gourami, high protein, UFBs-RAS

Abstract

Gourami (Osphronemus goramy) production in intensive systems is often suboptimal because of poor feeding management and water quality. The application of a recirculation system with ultrafine bubbles (UFBs-RAS) has the potential to improve the quality of the cultivation environment. However, feeding strategies, particularly the frequency and protein content, strongly influence their effectiveness. This study aimed to determine the optimal frequency of high-protein feeding on gourami fry production performance in the UFBs-RAS system. The study was conducted over 33 days. Feed was provided at 5% biomass/day with a protein content of ± 50% according to the treatment frequency. The test fish had a length of 3 ± 0.00 cm and a weight of 0.8 ± 0.00 g, with a total of 720 fish. This study was designed using a completely randomized design (CRD) with feeding frequencies of 3, 4, and 5 times per day. Each treatment was repeated 4 times, resulting in 12 experimental units. Statistical analysis showed that feeding frequency significantly affected the production performance of gourami fry. Feeding five times per day resulted in the best production performance, with a 100% survival rate, length growth rate of 3.83 ± 0.21%, weight growth rate of 4.16 ± 0.16%, feed utilization efficiency of 127%, protein efficiency ratio of 2.56, and size variation of 9.33%. These results indicate that increasing the feeding frequency in the UFBs-RAS system can improve the nutrient utilization efficiency and optimal growth performance of gourami fry.

Downloads

Download data is not yet available.

References

Abasubong KP, Liu WB, Zhang DD, Yuan XY, Xia SL, Xu C, Li XF. 2018. Fishmeal Replacement by Rice Protein Concentrate with Xylooligosaccharides Supplement Benefits the Growth Performance, Antioxidant Capability, and Immune Responses Against Aeromonas hydrophila in Blunt Snout Bream (Megalobrama amblycephala). Fish & Shellfish Immunology. 78: 177–186. DOI: https://doi.org/10.1016/j.fsi.2018.04.044.

Abdella B, Shokrak NM, Abozahra NA, Elshamy YM, Kadira HI, Mohamed RA. 2024. Aquaculture and Aeromonas hydrophila: A Complex Interplay of Environmental Factors and Virulence. Aquaculture International. 32: 7671–7681. DOI: https://doi.org/10.1007/s10499-024-01535-y.

Agustono A, Hadi M, Cahyoko Y. 2009. Pemberian Tepung Limbah Udang yang Difermentasi dalam Ransum Pakan Buatan terhadap Laju Pertumbuhan, Rasio Konversi Pakan, dan Kelangsungan Hidup Benih Ikan Nila (Oreochromis niloticus). Jurnal Ilmiah Perikanan dan Kelautan. 1(2): 157–162. DOI: https://doi.org/10.20473/jipk.v1i2.11682.

Andriyan MF, Rahmaningsih S, Firmani U. 2018. Pengaruh Salinitas terhadap Tingkat Kelangsungan Hidup dan Profil Darah Ikan Nila (Oreochromis niloticus) yang Diberi Kombinasi Pakan dan Buah Mengkudu (Morinda citriffolia L.). Jurnal Perikanan Pantura (JPP). 1(1): 1–9. DOI: https://doi.org/10.30587/jpp.v1i1.285.

Arief M, Fitriani N, Subekti S. 2014. Pengaruh Pemberian Probiotik Berbeda pada Pakan Komersial terhadap Pertumbuhan dan Efisiensi Pakan Ikan Lele Sangkuriang (Clarias sp.). Jurnal Ilmiah Perikanan dan Kelautan. 6(1): 49–54. DOI: https://doi.org/10.20473/jipk.v6i1.11381.

Azrita, Syandri H. 2018. Effects of Salinity on Survival and Growth of Gurami Sago (Osphronemus goramy, Lacepède, 1801) Juveniles. Pakistan Journal of Biological Sciences. 21(4): 171–178. DOI: https://doi.org/10.3923/pjbs.2018.171.178.

Azrita, Syandri H, Adnestasia L. 2020. Effects of Feeding Frequency on Growth Performance and Feed Conversion Ratio of Gurami Sago (Osphronemus goramy) Fingerlings in a Recirculating Aquaculture Pond System. The 8th International and National Seminar on Fisheries and Marine Science, 12 September 2019, Pekanbaru, Indonesia. IOP Conference Series: Earth and Environmental Science. DOI: https://doi.org/10.1088/1755-1315/430/1/012029.

Bidlack WR. 2000. Nutritional Biochemistry, 2nd Ed. Tom Brody. San Diego, 1999. Journal of the American College of Nutrition. 19(3): 419–420. DOI: https://doi.org/10.1080/07315724.2000.10718940.

Briggs MRD, Fling-Smith ST. 2002. The Effect of Zeolites and Other Alumino Silicate Clays on Water Quality at Various Salinities. Aquaculture Research. 27: 301–311. DOI: https://doi.org/10.1111/j.1365-2109.1996.tb01257.x.

[BSN] Badan Standardisasi Nasional. 2000. SNI 01-6485.3-2000. Produksi Benih Ikan Gurami (Osphronemus goramy, Lac) Kelas Benih Sebar. Jakarta.

Buentello JA, Gatlin DM, Neill NH. 2000. Effects of Water Temperature and Dissolved Oxygen on Daily Feed Consumption, Feed Utilization, and Growth of Channel Catfish (Ictalurus punctatus). Aquaculture. 182(3–4): 339–352. DOI: https://doi.org/10.1016/S0044-8486(99)00274-4.

Dirmansyah SY, Lumbessy, Lestari DP. 2022. Pengaruh Pemberian Kombinasi Pakan Pellet dan Pakan Hewani pada Budidaya Benih Ikan Gurami (Osphronemus gouramy). Journal of Fish Nutrition. 2(2): 148–160. DOI: https://doi.org/10.29303/jfn.v2i2.2071.

Durborow RM, Crosby DM, Brunson MW. 1997. Ammonia in Fish Ponds. Southern Regional Aquaculture Center (SRAC) Publication No. 463. The United States Department of Agriculture, Cooperative States Research, Education, and Extension Service.

Dwyer KS, Brown JA, Parrish C, Lall SP. 2002. Feeding Frequency Affects Food Consumption, Feeding Pattern, and Growth of Juvenile Yellowtail Flounder (Limanda ferruginea). Aquaculture. 213(1–4): 279–292. DOI: https://doi.org/10.1016/S0044-8486(02)00224-7.

Firdaus R, Syandri H, Azrita. 2024. Pengaruh Tingkat dan Frekuensi Pemberian Pakan terhadap Kinerja Pertumbuhan dan Pemanfaatan Pakan pada Pemeliharaan Benih Ikan Gurami (Osphronemus gouramy). Jurnal Riset Akuakultur. 19(4): 365–382. DOI: https://doi.org/10.15578/jra.19.4.2024.365-382.

[FAO] Food and Agriculture Organization. 2020. Aquaculture Management and Fish Survival Assessment. FAO Fisheries and Aquaculture Technical Paper. Rome (IT): FAO.

Fransiska F, Rachmawati D, Samidjan I. 2013. Pengaruh Persentase Jumlah Pakan Buatan yang Berbeda terhadap Pertumbuhan dan Kelulushidupan Keong Macan (Babylonia spirata L.). Journal of Aquaculture Management and Technology. 2(4): 122–130.

Fujaya Y. 2008. Dasar Pengembangan Teknik Perikanan. Jakarta(ID): Rineka Cipta.

Goddard S. 1996. Feed Management in Intensive Aquaculture. New York (US): Springer.

Gunawan H, Tang UM, Mulyadi M. 2019. Pengaruh Suhu Berbeda terhadap Laju Pertumbuhan dan Kelulushidupan Benih Ikan Selais (Kryptopterus lais). Jurnal Perikanan dan Kelautan. 24(2): 101–105.

Hanif IM, Effendi I, Budiardi T, Diatin I. 2021. Pengembangan Recirculated Aquaculture System (RAS) dengan Aplikasi Nanobubble untuk Meningkatkan Kinerja Pertumbuhan Benih Ikan Kerapu. Jurnal Akuakultur Indonesia. 20(2): 181–190. DOI: https://doi.org/10.19027/jai.20.2.181-190.

Hassan HU, Ali QM, Khan W, Masood Z, Abdel-Aziz MA, Shah MIA, Gabol K, Wattoo J, Chatta AM, Kamal M, et al. 2021. Effect of Feeding Frequency as a Rearing System on Biological Performance, Survival, Body Chemical Composition, and Economic Efficiency of Asian Seabass Lates calcarifer (Bloch, 1790) Reared Under Controlled Environmental Conditions. Saudi Journal of Biological Sciences. 28(12): 7360–7366. DOI: https://doi.org/10.1016/j.sjbs.2021.08.031.

Herawati VE, Agus M. 2014. Analisis Pertumbuhan dan Kelulushidupan Larva Lele (Clarias gariepenus) yang Diberi Pakan Daphnia sp. Hasil Kultur Massal Menggunakan Pupuk Organik Difermentasi. Pena: Jurnal Ilmu Pengetahuan dan Teknologi. 26(1): 1–11.

Hisano H, Pinheiro VR, Losekann ME, Moura e Silva MSG. 2020. Effect of Feeding Frequency on Water Quality, Growth, and Hematological Parameters of Nile tilapia (Oreochromis niloticus) Reared Using Biofloc Technology. Journal of Applied Aquaculture. DOI: https://doi.org/10.1080/10454438.2020.1715909.

Howerton R. 2001. Best Management Practices for Hawaiian Aquaculture. Waimanalo (US): Center for Tropical and Subtropical Aquaculture.

Huang M, Zhou Y, Chen X, Yang X, Gao Q, Dong S. 2020. Studies on Gastric Evacuation Rate and Feeding Frequency of Rainbow Trout (Oncorhynchus mykiss) in Two Sizes. Journal of Ocean University of China. 50: 33–39.

Ijaz N, Iqbal Z, Chaudhary JA. 2010. Effects of Ammonia on Growth of Ctenopharyngodon idella (Valenciennes) Fingerlings. Punjab University Journal of Zoology. 25(1–2): 59–66.

Khasani I, Febrianti R, Sularto, Pamungkas W, Rosada KK. 2024. Identification of Potential Molecular Markers for Disease Resistance in Giant Gourami through Major Histocompatibility Complex (MHC) II Gene Analysis. Fisheries and Aquatic Sciences. 27(3): 159–170. DOI: https://doi.org/10.47853/FAS.2024.e16.

[KKP] Kementerian Kelautan dan Perikanan. 2025. Volume Produksi Perikanan Budidaya Pembesaran Per Komoditas Utama. https://portaldata.kkp.go.id/portals/data-statistik/prod-ikan/tbl-statis/d/53. [June 12, 2025].

Lemarie G, Dosdat A, Coves D, Dutto G, Gasset E, Ruyet JP. 2004. Effect of Chronic Ammonia Exposure on Growth of European Seabass (Dicentrarchus labrax) Juveniles. Aquaculture. 229(1–4): 479–491. DOI: https://doi.org/10.1016/S0044-8486(03)00392-2.

Li L, Shen Y, Yang W, Xu X, Li J. 2021. Effect of Different Stocking Densities on Fish Growth Performance: A Meta-Analysis. Aquaculture. 544: 737152. DOI: https://doi.org/10.1016/j.aquaculture.2021.737152.

Li Y, Bordinhon AM, Davis DA, Zhang W, Zhu X. 2012. Protein: Energy Ratio in Practical Diets for Nile Tilapia Oreochromis niloticus. Aquaculture International. 21: 1109–1119. DOI: https://doi.org/10.1007/s10499-012-9616-3.

Mareta RE, Subandiyono S, Hastuti S. 2018. Pengaruh Enzim Papain dan Probiotik dalam Pakan terhadap Tingkat Efisiensi Pemanfaatan Pakan dan Pertumbuhan Ikan Gurami (Osphronemus gouramy). Sains Akuakultur Tropis: Indonesian Journal of Tropical Aquaculture. 1(1): 21–30. DOI: https://doi.org/10.14710/sat.v1i1.2452.

Marzuqi M, Astuti NWW, Suwirya K. 2012. Pengaruh Kadar Protein dan Rasio Pemberian Pakan terhadap Pertumbuhan Ikan Kerapu Macan (Epinephelus fuscoguttatus). Jurnal Ilmu dan Teknologi Kelautan Tropis. 4(1): 55–65.

Masser MP, Rakocy J, Thomas M, Losordo TM. 1999. Recirculating Aquaculture Tank Production Systems: Management of Recirculating Systems. Southern Regional Aquaculture Center (SRAC) Publication No. 452. The United States Department of Agriculture, Cooperative States Research, Education, and Extension Service.

Muin AJS, Nirmala K, Setiawati M, Hastuti YP. 2020. Pemanfaatan Perifiton pada Jumlah Substrat Berbeda terhadap Kualitas Air dan Kinerja Pertumbuhan Benih Ikan Gurami (Osphronemus gouramy). Jurnal Riset Akuakultur. 15(3): 165–173. DOI: https://doi.org/10.15578/jra.15.3.2020.165 173.

Nikhlani A, Pagoray H, Riang, A. 2025. Addition of Phytase in Artificial Feed Given to Giant Gourami Fish Fry (Osphronemus goramy). Jurnal Teknologi Perikanan dan Kelautan. 16(2): 212–220. DOI: https://doi.org/10.24319/jtpk.16.212-220.

Nirmala K, Rasmawan. 2010. Kinerja Pertumbuhan Ikan Gurami (Osphronemus goramy Lac.) yang Dipelihara pada Media Bersalinitas dengan Paparan Medan Listrik. Jurnal Akuakultur Indonesia. 9(1): 46–55. DOI: https://doi.org/10.19027/jai.9.46-55.

Olusola SE, Nwanna LC. 2014. Growth Performance of Nile Tilapia (Oreochromis niloticus) Fed Processed Soybean Meal-Based Diets Supplemented with Phytase. International Journal of Aquaculture. 4(8): 48–54.

Putra AW, Basuki F, Yuniarti T. 2016. Pengaruh Penambahan Recombinant Growth Hormone (rGH) pada Pakan dengan Kadar Protein Tinggi terhadap Pertumbuhan dan Tingkat Kelulushidupan Benih Ikan Gurami (Osphronemus gouramy). Journal of Aquaculture Management and Technology. 5(1): 17–25.

Rachmawati D, Hutabarat J, Fiat AI, Elfitasari T, Windarto S, Dewi EN. 2021. Penambahan Asam Amino Triptofan dalam Pakan terhadap Tingkat Kanibalisme dan Pertumbuhan Litopenaeus vannamei. Jurnal Kelautan Tropis. 24(3): 343–352. DOI: https://doi.org/10.14710/jkt.v24i3.11723.

Rambo, Yustiati A, Dhahiyat Y, Rostika R. 2018. Pengaruh Penambahan Tepung Biji Turi Hasil Fermentasi pada Pakan Komersial terhadap Pertumbuhan dan Kelangsungan Hidup Ikan Nila (Oreochromis niloticus). Jurnal Perikanan dan Kelautan. 9(1): 95–103.

Slembrouck J, Baras E, Subagja J, Hung LT, Legendre M. 2009. Survival, Growth, and Food Conversion of Cultured Larvae of Pangasianodon hypophthalmus, Depending on Feeding Level, Prey Density and Fish Density. Aquaculture. 294(1–2): 52–59. DOI: https://doi.org/10.1016/j.aquaculture.2009.04.038.

Subardi M, Muharam A, Juliana. 2016. Pemberian Pakan Buatan Berbahan Limbah Kepala Udang terhadap Pertumbuhan dan Sintasan Benih Ikan Gurami (Osphronemus gouramy). Jurnal Ilmiah Perikanan dan Kelautan. 4(1): 25–31.

Subhan U, Iskandar, Zahidah, Panatarani C, Joni IM. 2022. Effect of Ultrafine Bubbles on Various Stocking Density of Striped Catfish Larviculture in Recirculating Aquaculture System. Fishes. 7(4): 190. DOI: https://doi.org/10.3390/fishes7040190.

Subhan U, Iskandar I, Zahidah Z, Joni IM. 2021. Detection of Reserve Oxygen Potential in the Presence of Fine Bubbles and Its Ammonia Removal for Aquaculture Effluent. Materials Science Forum. 1044: 103–111. DOI: https://doi.org/10.4028/www.scientific.net/MSF.1044.103.

Subhan U, Mauladani S, Rosidah, Suryadi IBB, Joni IM. 2020. Promising Application of Fine Bubbles (FBs) to Control Aeromonas hydrophila on Striped Catfish Seed (Pangasianodon hypophthalmus). 2nd International Conference and Exhibition on Powder Technology (ICePTi), 20–21 August 2019, Solo, Indonesia. AIP Conference Proceedings. DOI: https://doi.org/10.1063/5.0003059.

Sucipto A, Prihartono RE. 2007. Pembesaran Nila Merah Bangkok: Di Karamba Jaring Apung, Kolam Air Deras, Kolam Air Tenang dan Karamba. Jakarta (ID): Penebar Swadaya.

Temesgen T, Bui TT, Han M, Kim T, Park H. 2017. Micro and Nanobubble Technologies as a New Horizon for Water-Treatment Techniques: A Review. Advances in Colloid and Interface Science. 246: 40–51. DOI: https://doi.org/10.1016/j.cis.2017.06.011.

Thorarensen H, Gústavsson A, Gunnarsson S, Árnason J, Steinarsson A, Björnsdóttir R, Imsland AKD. 2017. The Effect of Oxygen Saturation on the Growth and Feed Conversion of Juvenile Atlantic Cod (Gadus morhua L.). Aquaculture. 475: 24–28. DOI: https://doi.org/10.1016/j.aquaculture.2017.04.002.

Tsuge H. 2015. Micro- and Nanobubbles. Boca Raton (US): CRC Press, Taylor & Francis Group.

Wang H, Zhang L. 2017. Research on the Nitrogen Removal Efficiency and Mechanism of Deep Subsurface Wastewater Infiltration Systems by Fine Bubble Aeration. Ecological Engineering. 107: 33–40. DOI: https://doi.org/10.1016/j.ecoleng.2017.07.005.

Warwick EJ, Astuti JM, Hardjosubroto W. 1995. Pemuliaan Ternak. Yogyakarta (ID): Gadjah Mada University Press.

Wibawa YG, Amin M, Wijayanti M. 2018. Pemeliharaan Benih Ikan Gurami (Osphronemus gouramy) dengan Frekuensi Pemberian Pakan yang Berbeda. Jurnal Akuakultur Rawa Indonesia. 6(1): 28–36. DOI: https://doi.org/10.36706/jari.v6i1.7147.

Wibowo WP, Samidjan I, Rachmawati D. 2018. Analisis Laju Pertumbuhan Relatif, Efisiensi Pemanfaatan Pakan dan Kelulushidupan Benih Ikan Gurami (Osphronemus gouramy) melalui Substitusi Silase Tepung Bulu Ayam dalam Pakan Buatan. Sains Akuakultur Tropis: Indonesian Journal of Tropical Aquaculture. 2(1): 30–37.

Xing S, Liang X, Wang H, Xie X, Wierenga PA, Schrama JW, Xue M. 2023. The Impacts of Physical Properties of Extruded Feed on the Digestion Kinetics, Gastrointestinal Emptying and Stomach Water Fluxes of Spotted Seabass (Lateolabrax maculatus). Aquaculture. 570: 739442. DOI: https://doi.org/10.1016/j.aquaculture.2023.739442.

Yildiz HY, Robaina L, Pirhonen J, Mente E, Domínguez D, Parisi G. 2017. Fish Welfare in Aquaponic Systems: Its Relation to Water Quality With an Emphasis on Feed and Faeces—A Review. Water. 9(1): 13. DOI: https://doi.org/10.3390/w9010013.

Zakes Z, Kowalska A, Czerniak S, Demska-Zakęś K. 2006. Effect of Feeding Frequency on Growth and Size Variation in Juvenile Pikeperch, Sander lucioperca (L.). Czech Journal of Animal Science. 51(2): 85–91. DOI: https://doi.org/10.17221/3914-CJAS.

Zhang Y, Liang X-F, He S, Li L. 2021. Effects of Long-Term Low-Concentration Nitrite Exposure and Detoxification on Growth Performance, Antioxidant Capacities, and Immune Responses in Chinese Perch (Siniperca chuatsi). Aquaculture. 533: 736123. DOI: https://doi.org/10.1016/j.aquaculture.2020.736123.

Zonneveld N, Huisman EA, Boon JH. 1991. Prinsip-Prinsip Budidaya Ikan. Jakarta (ID): PT. Gramedia Pustaka Utama.

Downloads

Published

2026-05-07

Issue

Section

JTPK MAY 2026

How to Cite

Subhan, U., Zahra, N. F., Nurhayati, A., & Zidni, I. (2026). THE EFFECT OF HIGH-PROTEIN FEEDING FREQUENCY OF UFBs-RAS ON THE PRODUCTION PERFORMANCE OF GOURAMI (Osphronemus goramy) FRY: PENGARUH FREKUENSI PEMBERIAN PAKAN PROTEIN TINGGI PADA UFBs-RAS TERHADAP PERFORMA PRODUKSI BENIH GURAMI (Osphronemus goramy). Jurnal Teknologi Perikanan Dan Kelautan, 17(2), 174-185. https://doi.org/10.24319/jtpk.17.174-185