Metabolit dan Profil Darah Ayam Akhir Periode Bertelur yang Disuplementasi Enzim Bromelin dalam Ransumnya
Metabolite and Blood Profile of Late Laying Period Supplemented with Bromelain Enzyme in The Ration
Abstract
This experiment aimed to evaluate the effect of bromelain enzyme supplementation in a late-laying period diet on blood metabolite and blood profile parameters. A total of 200 Isa Brown at late laying period hens, 85 weeks of age, were randomly distributed into four dietary treatments and fed for eight weeks. The bromelain enzyme was used at the level of 0% (control diet), 0.025%, 0.050%, and 0.075%. Each treatment was replicated five times. Experimental design data were analyzed using ANOVA and Duncan multiple range test. Bromelain supplementation had a significant effect on the concentration of blood protein (p<0.01), reduced blood cholesterol (p<0.05), and increased concentration of immunoglobulin Y (IgY) (p<0.05). It is concluded that supplementation at dosages 0.05% and 0.075% of bromelain can improve the birds' immunity by increasing the IgY content in blood serum. supplementation at 0.05% reduces blood cholesterol and increases blood protein.
Key words: blood metabolite, blood profile, bromelain, IgY, late laying period
Downloads
References
Adewole FA, Egbeyale LT, Ekunseitan DA, Bello KO, Lala OA & Famakinde SA. 2021. Effect of strain and sex on haematological and serum biochemical indices of tropical indigenous chickens. Nigerian Journal of Animal Production 48 (2): 18-26.
Agrawal P, Nikhade P, Patel A, Mankar N & Sedani S. 2022. Bromelain: a potent phytomedicine. Cureus 14(8): e27876. https://doi.org/10.7759/cureus.27876
Chen CH, Hsia CC, Hu PA, Yeh CH, Chen CT, Peng CL, Wang CH, & Lee TS. 2022. Bromelain ameliorates atherosclerosis by activating the TFEB-Mediated autophagy and antioxidant pathways. Antioxidants 12(1): 72. https://doi.org/ 10.3390/antiox12010072
Chakraborty AJ, Mitra S, Tallei TE, Tareq AM, Nainu F, Cicia D, Dhama K, Emran TB, Simal-Gandara J & Capasso R. 2021. Bromelain a potential bioactive compound: a comprehensive overview from a pharmacological perspective. Life 11(4): 317. https://doi.org/10.3390/life11040317
Clinical Diagnostic Division. 1990. Veterinary Reference Guide. New York (US):Eastman Kodak Company
Dave S, Kaur NJ, Nanduri R, Dkhar HK, Kumar A & Gupta P. 2012. Inhibition of adipogenesis and induction of apoptosis and lipolysis by stem bromelain in 3T3-L1 adipocytes. PLoS One. 7(1): e30831.
Dong XY, Azzam MMM, & Zou XT. 2016. Effects of dietary L-isoleucine on laying performance and immunomodulation of laying hens. Poultry Science 00:1–9. http://dx.doi.org/10.3382/ps/pew163
Gomperts BD, Kramer IM & Tatham PER. 2009. Signal Transductions. San Diego (US) : Elsevier
Harlap SY, Kadirov NN, Gorelik LS, Meshcheryakova GV, & Mukhamedyarova LG. 2021. Age-related variability of indicators of protein metabolism in the blood of laying hens. IOP Conf. Series: Earth and Environmental Science 677 (2021) 042019. IOP Publishing Ltd.
Hendarto T & Budiyanto D. 2022. Anticholesterol bioactive peptides from bromelain hydrolysates of mangrove sonneratia alba protein. International Journal of Multidisciplinary Research and Analysis 5 (12):3589-3597. https://doi.org/10.47191/ijmra/v5-i12-39
Hossain MM, Lee SI, & Kim IH. 2015. Effects of bromelain supplementation on growth performance, nutrient digestibility, blood profiles, faecal microbial shedding, faecal score and faecal noxious gas emission in weanling pigs. Veterinarni Medicina, 60(10): 544–552. https://doi.org/10.17221/8493-VETMED
Hu PA, Chen CH, Guo BC, Yu RK & Lee TS. 2020. Bromelain confers protection against the non-alcoholic fatty liver disease in male C57BL/6 mice. Nutrients 12(5): 1458 doi:10.3390/nu12051458
Jain NC. 1993. Essential of Veterinary Hematology. Philadelphia.(US): Lea & Febiger.
Ketnawa S, Chaiwut P & Raudkeun S. 2012. Pineapple wastes: a potential source for bromelain extraction. Food and Bioproducts Processing.90 (3): 385–391 https://doi.org/10.1016/j.fbp.2011.12.006
Kritis P, Karampela I, Kokoris S & Dalamaga M. 2020. The combination of bromelain and curcumin as an immune-boosting nutraceutical in the prevention of severe COVID-19. Metabolism Open 8 : 100066. https://doi.org/10.1016/j.metop.2020.100066
Li GM, Liu LP, Yin B, Liu YY, Dong WW, Gong S, Zhang J, & Tan JH. 2020. Heat stress decreases egg production of laying hens by inducing apoptosis of follicular cells via activating the FasL/Fas and TNF-α systems. Poultry Science. 99 (11): 6084-6093 https://doi.org/10.1016/j.psj.2020.07.024
Lien TF, Cheng YH & Wu CP. 2012. Effects of supplemental bromelain on egg production and quality, Serum and liver traits of laying hens. Journal of Animal Science Advances, 2(4): 386–391.
Liu Z, Sun C, Yan Y, Li G, Shi F, Wu G, Liu A & Yang N. 2018. Genetic variations for egg quality of chickens at late laying period revealed by genome-wide association study. Science Report. 8:10832
Pawar SS, Sajjanar B, Lonkar VD, Kurade NP, Kadam AS, Nirmal AV, Brahmane MP & Bal SK. 2016. Assessing and mitigating the impact of heat stress on poultry. Advances in Animal and Veterinary Sciences. 4(6): 332–341.
Sastradipraja D, Sikar SHS, Wijayakusuma R, Ungerer T, Maad A, Nasution H, Suriawinata R & Hamzah R. 1989. Penuntun Praktikum Fisiologi Veteriner. Pusat Antar Universitas Ilmu Hayati. Bogor (ID): Institut Pertanian Bogor
Sahbaz A, Aynioglu O, Isik H, Ozmen U, Cengil O, Gun BD & Gungorduk K. 2015. Bromelain: a natural proteolytic for intra-abdominal adhesion prevention. International Journal of Surgery 14: 7-11. http://dx.doi.org/10.1016/j.ijsu.2014.12.024
Swenson MJ, & William OR. 1993. Duke`s Physiology of Domestic Animals. Ed ke-11. Ithaca and London. (US): Publishing Assocattes a Division of Cornell University,
Trinder P. 1969. Recommended method for the determination of cholesterol and triglyceride in blood. Annals of Clinical Biochemistry 6: 24-27.
Tao Y, Wang T, Huang C, Lai C, Ling Z, Zhou Y & Yong Q. 2021. Production performance, egg quality, plasma biochemical constituents and lipid metabolites of aged laying hens supplemented with incomplete degradation products of galactomannan. Poultry Science 100 (80:101296. https://doi.org/10.1016/j.psj.2021.101296
Umar S, Munir MT, Ahsan U, Raza I, Chowdhury MR, Ahmed Z & Shah MAA. 2017. Immunosuppressive interactions of viral diseases in poultry. World's Poultry Science Journal 73 (1) :121-135. doi:10.1017/S0043933916000829
Varilla C, Marcone M, Paiva L & Baptista J. 2021. Bromelain, a group of pineapple proteolytic complex enzymes (ananas comosus) and their possible therapeutic and clinical effects. A summary. Foods 10 (10) :2249. https://doi.org/10.3390/foods10102249
Wasti S, Sah N & Mishra B. 2020. Impact of heat stress on poultry health and performances, and potential mitigation strategies. MDPI. Animals 10 (8): 1266 doi:10.3390/ani10081266
Wu YN, Yan FF, Hu JY, Chen H, Tucker CM, Green AR & Cheng HW. 2017. The effect of chronic ammonia exposure on acute-phase proteins, immunoglobulin, and cytokines in laying hens. Poultry Science 96 (6): 1524-1530 http://dx.doi.org/10.3382/ps/pew454
Yenice G, Iskender H, Dokumacioglu E, Kaynar O, Kaya A, Hayirli A, dan Sezmis G. 2019. Dietary bromelain supplementation for improving laying performance, egg quality and antioxidant status. Europian Poultry Science 83: 1–16. https://doi.org/10.1399/eps.2019.272
Yokoi K, Kobayashi F, Sakai J, Usui M & Tsuji M. 2002. Sandwich ELISA detection of excretory-secretory antigens of toxocara canis larvae using a specific monoclonal antibody. The Southeast Asian journal of Tropical Medicine and Public Health 33 (1) :33-37
Zainudin MN & Wahid A. 2019. Dietary bromelain improves nutrient digestibility, digesta viscosity and intestinal villus height as well as reduces intestinal E. coli population of broiler chickens. Malaysia Journal of Animal Science 22: 1–16.
Copyright (c) 2023 H Rafis, A Sudarman, Rita Mutia
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors of the submitted manuscript have to understand and agree that the copyrights published are held by Jurnal Ilmu Nutrisi dan Teknologi Pakan. Copyrights includes rights in reproducing, distributing and selling every section of articles in all forms and media. The copyright transfer form is signed by the corresponding author. The author”
• Creative Commons Attribution (CC BY)
you are allowed to:
Share – copy and redistribute the material in any medium or format
Adapt – remix, transform, and build upon the material
for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal)
This work is licensed under a Creative Commons Attribution 4.0 International License.
