Kecernaan Kalsium dan Fosfor, pH gizzard, Kadar Air Ekskreta dan Digesta pada Ayam Petelur yang Diberi Ukuran Partikel Batu Kapur Berbeda dan Enzim Fitase
Digestibility of Calcium and Phosphorus, Gizzard PH, Excreta and Digesta Moisture Content in Laying Hens on Different Limestone Particle Size and Phytase
Abstract
This study aimed to determine the coefficient of total calcium and phosphorus digestibility effects from limestone as a source of calcium and the effect of phytase enzymes on laying hens. This study used 180 Hy-line Brown laying hens aged 75 weeks. The experimental design used was a 2x2 factorial complete randomized design (CRD) with 9 replications. The first factor was limestone particle size, i.e., P1 = limestone flour and P2 = limestone granules, and the second factor was phytase enzyme dose, i.e., Q1 = 0 FTU and Q2 = 1000 FTU. The observed variables were gizzard pH, excreta moisture content (MC), digesta MC, calcium and phosphorus digestibility using two samples and ileal digestibility. The results showed that the digestibility of calcium from limestone flour was significantly different compared to limestone granules at P<0.05. The phytase addition and the interaction with limestone particle size did not affect gizzard pH, total and ileal digestibility of calcium and phosphorus. Excreta and digesta MC had a significant effect on the particle size of limestone, the highest MC was limestone granules compared to flour (P<0.001). The addition of phytase had an effect on reducing excreta MC (P< 0.001). There was interaction between limestone size and phytase in excreta and digesta MC. The conclusion of the research was that laying hens were more efficient in digesting calcium from limestone flour compared to limestone granules. Phytase could reduce excreta and digesta MC.
Key words: calcium, digestibility, limestone, particle size, phytase
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Abd El-Hack M, Alagawany M, Arif M, Emam M, Saeed M, Arain M, Siyal F, Patra A, Elnesr S, & Khan R. 2018. The uses of microbial phytase as a feed additive in poultry nutrition – a review. Annals of Animal Science. 18(3): 639-658.
Adedokun SA, Peskalsiumtore AJ, Ford MJ Ao T, & Jacob JP. 2018. Investigating the effect of dietary calcium levels on ileal endogenous amino acid losses and standardized ileal amino acid digestibility in broilers and laying hens. Poultry Science. 97(1): 131-139. https://doi.org/10.3382/ps/pex271
AOAC. 2016. Official Methods of Analysis. 20th ed. Washington DC(ID): Association of Official Analytical Chemists.
An SH, Sung JY, & Kong, C. 2020. Ileal digestibility and total tract retention of phosphorus in inorganic phosphates fed to broiler chickens using the direct method. Animals 10(11): 2167.
Anwar MN, Ravindran V, Morel C.H, Ravindran G, & Cowieson, AJ. 2016. Effect of limestone particle size and calcium to non-phytate phosphorus ratioon true ileal calcium digestibility of limestonefor broiler chickens. British Poultry Science, 57(5): 707-713.
Arguelles M, Nusairat B, Qudsieh R, & Brake J. 2020. Effects of phytase inclusion in broiler breeder diets during early lay on their fecal and egg characteristics. Brazilian Journal of Poultry Science. 22(1): 1-6
Bougouin A, Appuhamy JADRN, Kebreab E, Dijkstra J, Kwakkel RP, & France J. 2014. Effects of phytase supplementation on phosphorus retention in broilers and layers: a meta-analysis Poultry Science, 93(8): 1981-1992.
Browning LC, & Cowieson AJ. 2013. The concentration of strontium and other minerals in animal feed ingredients. Journal of Applied Animal Nutrition 2: 1-6.
David LS, Abdollahi MR, Ravindran G, Walk CL, & Ravindran V. 2019. Studies on the measurement of ileal calcium digestibility of calcium sources in broiler chickens. Poultry Science 98 (11): 5582-5589.
David LS, AbdollahiMR, Bedford MR, & Ravindran V. 2021. Comparison of the apparent ileal calcium digestibility of limestone in broilers and layers, BritishPoultryScience 62(6): 852-857
David LS, Anwar MN, Abdollahi MR, Bedford MR & Ravindran V. 2023. Calcium nutrition of broilers: current perspectives and challenges. Animals. 13(10):1590.
Dersjant-Li Y, Archer G, Stiewert AM, Brown AA, Sobotik EB, Jasek A, Marchal L, Bello A, Sorg RA, Christensen T, Kim HS, Mejldal R, Nikolaev I, Pricelius S, Haaning S, Sorensen JF, DeKreij A, & Sewalt V. 2020. Funcitionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to broilers fed a corn-soybean meal-based diet. Animal Feed Science and Technology. 264: 1-7.
Dersjant-Li Y, Millan C, Casabuena O, Quiles A, Romero LF, & Gracia MI. 2018. Supplementation of Buttiauxella sp. 6-phytase to commercial laying hen diets with reduced nutrient density on productive performance and eggquality. Journal of Applied Animal Nutrition 6 (e4):1–8.
Diana TF, Calderano AA, Tavernari FC, Rostagno HS, Teixeira AO, & Albino LFT. 2021. Age and calcium sources in laying hen feed affect calcium digestibility. Open Journal of Animal Sciences 11(3): 501-513.
Diana TF, Calderano AA, Rostagno HS, Rita M, Marques DL, De F, Tavernari C, Veroneze R, Fernando L, & Albino T. 2023. Apparent calcium retention and digestibility coefficients of limestone with different particle sizes in laying hens. Animal Science and Pastures, Scientia Agricola. (Piracicaba, Braz.) 80 : 1- 6
Ege G, Bozkurt M, Koçer B, Tüzün AE, Uygun M & Alkan G. 2019. Influence of feed particle size and feed form on productive performance, egg quality, gastrointestinal tract traits, digestive enzymes, intestinal morphology, and nutrient digestibility of laying hens reared in enriched cages. Poultry Science 98(9):3787-3801.
Świątkiewicz S, Arczewska-Włosek A, & Józefiak D. 2017. The nutrition of poultry as a factor affecting litter quality and foot pad dermatitis – an updated review. Journal of Animal Physiology and Animal Nutrition, 101: e14–e20.
Gilani, S, Mereu A, Li W, Plumstead PW, Angel R, Wilks G, & Y. Dersjant-Li Y. 2022. “Global survey of limestone used in poultry diets: Calcium content, particle size and solubility. Journal of Applied Animal Nutrition 10 (1): 19–30.
Kaswarjono, YY, Nururrozi, A, Soedarmanto, Indarjulianto S. 2016. Fitat dan fitase: dampak pada hewan ternak. Jurnal Ilmu-Ilmu Peternakan. 26 (3): 59-78.
Kim SW, Li W, Angel R, & Proszkowiec-Weglarz M. 2018. Effects of limestone particle size and dietary Ca concentration on apparent P and Ca digestibility in the presence or absence of phytase. Poultry Science 97 (12): 4306-4314.
Kim SW, Li W, Angel R & Plumstead PW. 2019. Modification of a limestone solubility method and potential to correlate with in vivo limestone calcium digestibility. Poultry Science 98 (12): 6837-6848.
Lalpanmawia H, Elangovan AV, Sridhar M, Shet D, Ajit h SP & al DT. 2014.Efficacy of phytase on growth performance, nutrient utilization and bone mineralization in broilerchicken. Animal Feed Science and Technology 192: 81–89.
Lee SA, Febery E, Mottram T & Bedford MR. 2021. Growth performance, real-time gizzard pH and calcium solubility in the gut of broiler chickens is dependent on the interaction between dietary calcium concentration and limestone particle size. British Poultry Science. 62(6):827-834.
Li X, Zhang D & Bryden WL. 2017. Calcium and phosphorus metabolism and nutrition of poultry: Are current diets formulated in excess?. Animal Production Science 57 (11) : 2304-2310
Liu SY, Truong HH, & Selle, PH. 2015. Whole-Grain Feeding for Chicken-Meat Production: Possible Mechanisms Driving Enhanced Energy Utilisation and Feed Conversion. Animal Production Science, 55(5): 559-572.
Mutucumarana RK, Ravindran V, Ravindran G & Cowieson AJ. 2014. Measurement of true ileal digestibility and total tract retention of phosphorus in corn and canola meal for broiler chickens. Poultry Science 93(2): 412-419.
Nusairat B, Arguelles M & Brake, J. 2017. Effect of the phytase inclusion in broiler breeder diets on fecal and egg characteristics. International Journal of Poultry Science. 17(1): 1-7.
NRC. 1994. Nutrient Requirements of Poultry, 9th rev. ed. Washington, DC (US): National Academy Press,
Proszkowiec-Weglarz M & Angel R. 2013. Calcium and phosphorusmetabolism in broilers: effect of homeostatic mechanism on calciumand phosphorus digestibility. Journal of Applied Poultry Research 22(3) : 609–627.
Rostagno HS, Albino LFT, Hannas MI, Donzele JL, Sakomura NK, Perazzo FG & Brito, CO. 2017. Brazilian Tables for Poultry and Swine: Feed Composition and Nutritional Requirements. Santiago (CL) :Department of Animal Science-UFV, Viçosa, 488 p.
Sahin K, Orhan C, Tuzcu M, Hayirli A, Komorowski JR & Sahin N. 2018. Effects of dietary supplementation of argininesilicate-inositol complex on absorption and metabolism of calcium of laying hens. PloS One 13(1): e0189329.
Shin S, Ha NC, Oh BC, Oh TK, & Oh BH. 2001. Enzyme mechanism and catalytic property of beta propeller phytase. Structure. 9(9): 851-858.
Truong HH, Bold RM, Liu SY, & Selle PH. 2015. Standard phytase inclusion in maize-based broiler diet enhances digestibility coefficient of starch, amino acids, and sodium in four small intestinal segments and digestive dynamics of starch and protein. Animal Feed Science and Technology. 209: 240-248.
Truong HH, Moss AF, Liu, SY, & Selle, P.H. 2017. Pre- and post-pellet whole grain inclusions enhance feed conversion efficiency, energy utilisation and gut integrity in broiler chickens offered wheat-based diets. Animal Feed Science and Technology, 224:115-123.
Van Dijk KC, Lesschen JP & Oenema O. 2016. Phosphorus flows and balances of the European union member states. Science of The Total Environment. 542 (2): 1078-1093
Walk C L, Bedford MR & Mcelroy AP. 2012. Influence of limestone and phytase on broiler performance, gastrointestinal pH, and apparent ileal nutrient digestibility. Poultry Science 91(6): 1371–1378.
Wilkinson SJ, Ruth B, & Cowieson AJ. 2013. Mineral Composition of Calcium Sources Used by the Australian Poultry Feed Industry. 24th Annual Australian Poultry Science Symposium, 24: 45-47.
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