Methods for assessing DNA fragmentation in bovine spermatozoa: A Mini-review of Indonesian research
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This article reviews research methods and analyses of bovine sperm DNA fragmentation conducted in Indonesia. A literature review using Google Scholar identified 37 articles by Indonesian authors published since 2018, with a peak of eight articles in 2023. The primary techniques employed include Acridine Orange staining (17 articles), Sperm-Bos-Halomax kits (12 articles), Toluidine Blue staining (7 articles), and the TUNEL assay (1 article), each exhibiting methodological variations. This review provides a valuable reference for stakeholders aiming to develop standardised regulations for semen evaluation in cattle.
Arif AA, Maulana T, Kaiin EM, Purwantara B, Arifiantini RI, Memili E. 2020. Comparative analysis of various step-dilution techniques on the quality of frozen semen. Veterinary World. 13(11):2422-2428. https://doi.org/10.14202/vetworld.2020.2422-2428 | PMid:33363336 PMCid:PMC7750238 DOI: https://doi.org/10.14202/vetworld.2020.2422-2428
Baity AN, Maghfiroh NA, Fitriana SB, Prihantoko KD, Maharani D, Widayati DT. 2024. Effect of storage periods on DNA fragmentation of post-thawed Bali bull sperm. Advance of Animal Veterinary Sci-ences. 12(8):1456-1464. https://doi.org/10.17582/journal.aavs/2024/12.8.1456.1464 DOI: https://doi.org/10.17582/journal.aavs/2024/12.8.1456.1464
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Pardede BP, Maulana T, Kaiin EM, Agil M, Karja NWK, Sumantri C, Supriatna. 2021. The potential of sperm bovine protamine as a protein marker of semen production and quality at the nation artificial insemination center of Indonesia. Veterinary World. 14(9): 2473-2481. https://doi.org/10.14202/vetworld.2021.2473-2481 | PMid:34840468 PMCid:PMC8613797 DOI: https://doi.org/10.14202/vetworld.2021.2473-2481
Pardede BP, Supriatna I, Yudi Y, Agil M. 2020. Decreased bull fertili-ty: age-related changes in sperm. E3S Web of Conferences, 1st ICVAES 2019. 151:01010. https://doi.org/10.1051/e3sconf/202015101010 DOI: https://doi.org/10.1051/e3sconf/202015101010
Prabowo TA, Bintara S, Yusiati LM, Sitaresmi PI, Widayati D. 2023. Evaluation DNA fragmentation of local Indonesian cattle frozen sperm using Halomax® method. Biodiversitas. 24(4):2225-2230. https://doi.org/10.13057/biodiv/d240435 DOI: https://doi.org/10.13057/biodiv/d240435
Prihantoko KD, Kusumawati A, Widayati DT, Pangestu M. 2020. Effects of storage duration on mitochondrial activity and DNA fragmentation of post-thawed spermatozoa from several ongole grade bull in Indonesia. Veterinary Practitioner. 21(2):264-268.
Priyanto L, Budiyanto A, Kusumawati A, Kurniasih, Arifiantini I. 2018. Perbandingan pemeriksaan kerusakan DNA spermatozoa post thawing antara Sperm-Bos-Halomax® dan Toluidine Blue. Jurnal Pe-ternakan Sriwijaya. 7(1):30-39. https://doi.org/10.33230/JPS.7.1.2018.7081 DOI: https://doi.org/10.33230/JPS.7.1.2018.7081
Said S, Maulana T, Setiorini S, Ibrahim G, Ramadhan M, Christophe E. 2020. Effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Journal of the Indonesian Tropical Ani-mal Agriculture. 45(3):189-196. https://doi.org/10.14710/jitaa.45.3.189-196 DOI: https://doi.org/10.14710/jitaa.45.3.189-196
Satrio A, Karja NWK, Setiadi MA, Kaiin EM, Pardede BP, Purwantara B. 2024. Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull. Frontiers in Veterinary Science. 11:1393706. https://doi.org/10.3389/fvets.2024.1393706 | PMid:39183752 PMCid:PMC11343614 DOI: https://doi.org/10.3389/fvets.2024.1393706
Satrio FA, Karja NWK, Setiadi MA, Kaiin EM, Kurnia A, Purwantara B. 2022. Productivity and fresh semen characteristics of Simmental bulls of different ages. Jurnal Kedokteran Hewan. 16(1):23-28. https://doi.org/10.21157/j.ked.hewan.v16i1.23487 DOI: https://doi.org/10.21157/j.ked.hewan.v16i1.23487
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