Methods for assessing DNA fragmentation in bovine spermatozoa: A Mini-review of Indonesian research
Artikel ini mengulas metode penelitian dan analisis fragmentasi DNA sperma sapi yang dilakukan di Indonesia. Tinjauan pustaka menggunakan Google Scholar mengidentifikasi 37 artikel oleh penulis Indonesia yang diterbitkan sejak 2018, dengan puncak delapan artikel pada 2023. Teknik utama yang digunakan meliputi pewarnaan Acridine Orange (17 artikel), kit Sperm-Bos-Halomax (12 artikel), pewarnaan Toluidine Blue (7 artikel), dan uji TUNEL (1 artikel), yang masing-masing menunjukkan variasi metodologis. Tinjauan ini memberikan referensi berharga bagi para pemangku kepentingan yang ingin mengembangkan peraturan standar untuk evaluasi sperma pada sapi.
Arif AA, Maulana T, Kaiin EM, Purwantara B, Arifiantini RI, Memili E. 2020. Comparative analysis of various step-dilution techniques on the quality of frozen semen. Veterinary World. 13(11):2422-2428. https://doi.org/10.14202/vetworld.2020.2422-2428 | PMid:33363336 PMCid:PMC7750238 DOI: https://doi.org/10.14202/vetworld.2020.2422-2428
Baity AN, Maghfiroh NA, Fitriana SB, Prihantoko KD, Maharani D, Widayati DT. 2024. Effect of storage periods on DNA fragmentation of post-thawed Bali bull sperm. Advance of Animal Veterinary Sci-ences. 12(8):1456-1464. https://doi.org/10.17582/journal.aavs/2024/12.8.1456.1464 DOI: https://doi.org/10.17582/journal.aavs/2024/12.8.1456.1464
Pardede BP, Agil M, Karja NWK, Sumantri C, Supriatna I, Purwantara. 2022. PRM1 gene expression and its protein abundance in frozen-thawed spermatozoa as potential fertility markers in breeding bulls. Veterinary Sciences. 9(3): 111. https://doi.org/10.3390/vetsci9030111 | PMid:35324839 PMCid:PMC8951773 DOI: https://doi.org/10.3390/vetsci9030111
Pardede BP, Agil M, Yudi Y, Supriatna I. 2020b. Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross breeding program. Veterinary World. 13(12):2649-2657. https://doi.org/10.14202/vetworld.2020.2649-2657 | PMid:33487983 PMCid:PMC7811557 DOI: https://doi.org/10.14202/vetworld.2020.2649-2657
Pardede BP, Maulana T, Kaiin EM, Agil M, Karja NWK, Sumantri C, Supriatna. 2021. The potential of sperm bovine protamine as a protein marker of semen production and quality at the nation artificial insemination center of Indonesia. Veterinary World. 14(9): 2473-2481. https://doi.org/10.14202/vetworld.2021.2473-2481 | PMid:34840468 PMCid:PMC8613797 DOI: https://doi.org/10.14202/vetworld.2021.2473-2481
Pardede BP, Supriatna I, Yudi Y, Agil M. 2020. Decreased bull fertili-ty: age-related changes in sperm. E3S Web of Conferences, 1st ICVAES 2019. 151:01010. https://doi.org/10.1051/e3sconf/202015101010 DOI: https://doi.org/10.1051/e3sconf/202015101010
Prabowo TA, Bintara S, Yusiati LM, Sitaresmi PI, Widayati D. 2023. Evaluation DNA fragmentation of local Indonesian cattle frozen sperm using Halomax® method. Biodiversitas. 24(4):2225-2230. https://doi.org/10.13057/biodiv/d240435 DOI: https://doi.org/10.13057/biodiv/d240435
Prihantoko KD, Kusumawati A, Widayati DT, Pangestu M. 2020. Effects of storage duration on mitochondrial activity and DNA fragmentation of post-thawed spermatozoa from several ongole grade bull in Indonesia. Veterinary Practitioner. 21(2):264-268.
Priyanto L, Budiyanto A, Kusumawati A, Kurniasih, Arifiantini I. 2018. Perbandingan pemeriksaan kerusakan DNA spermatozoa post thawing antara Sperm-Bos-Halomax® dan Toluidine Blue. Jurnal Pe-ternakan Sriwijaya. 7(1):30-39. https://doi.org/10.33230/JPS.7.1.2018.7081 DOI: https://doi.org/10.33230/JPS.7.1.2018.7081
Said S, Maulana T, Setiorini S, Ibrahim G, Ramadhan M, Christophe E. 2020. Effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Journal of the Indonesian Tropical Ani-mal Agriculture. 45(3):189-196. https://doi.org/10.14710/jitaa.45.3.189-196 DOI: https://doi.org/10.14710/jitaa.45.3.189-196
Satrio A, Karja NWK, Setiadi MA, Kaiin EM, Pardede BP, Purwantara B. 2024. Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull. Frontiers in Veterinary Science. 11:1393706. https://doi.org/10.3389/fvets.2024.1393706 | PMid:39183752 PMCid:PMC11343614 DOI: https://doi.org/10.3389/fvets.2024.1393706
Satrio FA, Karja NWK, Setiadi MA, Kaiin EM, Kurnia A, Purwantara B. 2022. Productivity and fresh semen characteristics of Simmental bulls of different ages. Jurnal Kedokteran Hewan. 16(1):23-28. https://doi.org/10.21157/j.ked.hewan.v16i1.23487 DOI: https://doi.org/10.21157/j.ked.hewan.v16i1.23487
Susilowati S, Ardjito T, Mustofa I, Widodo OS, Kurnijasanti R. 2021. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Animal Bioscience. 34(2):198-204. https://doi.org/10.5713/ajas.20.0025 | PMid:32299169 PMCid:PMC7876723 DOI: https://doi.org/10.5713/ajas.20.0025
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