FORMULATION DEVELOPMENT OF A PREGNANCY SUPPLEMENT BASED ON TUNA EYE OIL USING MIXTURE DESIGN: PENGEMBANGAN FORMULASI SUPLEMEN KEHAMILAN BERBASIS MINYAK IKAN MATA TUNA MENGGUNAKAN MIXTURE DESIGN

Wini Trilaksani; Silva Fauziah; Utami Dyah Syahfitri; Bambang Riyanto

doi:10.24319/jtpk.16.244-254

Authors

Wini Trilaksani Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPB University, Jl. Agatis, IPB Dramaga Campus, Bogor 16680, Indonesia
Silva Fauziah Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPB University, Jl. Agatis, IPB Dramaga Campus, Bogor 16680, Indonesia
Utami Dyah Syahfitri Department of Statistics, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Meranti, IPB Dramaga Campus, Bogor 16680, Indonesia
Bambang Riyanto Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPB University, Jl. Agatis, IPB Dramaga Campus, Bogor 16680, Indonesia

DOI:

https://doi.org/10.24319/jtpk.16.244-254

Keywords:

DHA, katsuobushi, mixture design, pregnancy supplement, tuna eye oil

Abstract

Stunting is a chronic nutritional problem with a high prevalence in Indonesia, with inadequate intake of protein and DHA during pregnancy being among the primary causes. This study aims to develop and optimize a pregnancy supplement formulation based on tuna eye oil and katsuobushi protein hydrolysate using a mixture design approach. The research stages included the extraction and characterization of fish oil from tuna eyeballs, the enzymatic hydrolysis of katsuobushi protein using papain, and the formulation of an emulsion using egg yolk as the emulsifier. Optimization was conducted using a simplex-lattice {3,1} design with axial points via Minitab 19 software. The optimal formulation consisted of 40.40% tuna eye oil, 31.02% katsuobushi protein hydrolysate, and 28.58% egg yolk, resulting in a pH of 4.29 and a viscosity of 3,880 cPs. The final product delivered 210 mg DHA per day, fulfilling the recommended daily intake for pregnant women. Nutritional profile and microbial safety tests also met established standards. These findings highlight the potential of tuna eye oil and katsuobushi-based supplements as an innovative nutritional intervention for stunting prevention.

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References

Abachi S, Bazinet L, Beaulieu L. 2019. Antihypertensive and Angiotensin-I-Converting Enzyme (ACE)-Inhibitory Peptides from Fish as Potential Cardioprotective Compounds. Marine Drugs. 17(11): 613. DOI: https://doi.org/10.3390/md17110613.

Amertaningtyas D, Jaya F. 2011. Sifat Fisiko Kimia Mayonnaise dengan Berbagai Tingkat Konsentrasi Minyak Nabati dan Eggyolk Ayam Buras. Jurnal Ilmu-Ilmu Peternakan. 21(1): 1-6.

Anggraini VR. 2018. Aktivitas Inhibitor ACE dari Hidrolisat Peptida Katsuobushi Tradisional [Thesis]. Bogor (ID): Institut Pertanian Bogor.

[AOAC] Association of Official Analytical Chemist. 2005. Official Methods of Analysis of the Association of Official Analytical of Chemist International. Gaithersburg (US): AOAC International.

[AOCS] American Oil Chemists Society. 1997. Official Methods and Recommended Practices of AOCS International. Champaign (US): AOCS Press.

[AOCS] American Oil Chemists Society. 1998. Free Fatty Acids. In: Official Methods and Recommended Practices of the American Oil Chemists Society. Champaign (US): AOCS Press

Badan Penelitian dan Pengembangan Kesehatan. 2018. Data Riset Kesehatan Dasar (Riskesdas) 2018. Jakarta.

Bezerra MA, Lemos VA, Novaes CG, de Jesus RM, Filho HRS, Araújo SA, Alves JPS. 2020. Application of Mixture Design in Analytical Chemistry. Microchemical Journal. 152: 1-13. DOI: https://doi.org/10.1016/j.microc.2019.104336.

Blois MS. 1958. Antioxidant Determination by The Use of a Stable Free Radical. Nature. 181: 1199-1200. DOI: https://doi.org/10.1038/1811199a0.

[BSN] Badan Standardisasi Nasional. 1998. SNI 01-4473-1998. Mayones. Jakarta.

[BSN] Badan Standardisasi Nasional. 2009a. SNI 2691.1:2009. Ikan Kayu. Jakarta.

[BSN] Badan Standardisasi Nasional. 2009b. SNI 7388:2009. Batas Maksimum Cemaran Mikroba dalam Pangan. Jakarta.

[BSN] Badan Standardisasi Nasional. 2013. SNI 2729:2013. Ikan Segar. Jakarta.

[BSN] Badan Standardisasi Nasional. 2021. SNI 2354-23:2021. Cara Uji Kimia – Bagian 23: Penentuan Logam Berat: Merkuri (Hg), Timbal (Pb), Kadmium (Cd), Arsen (As), dan Timah Putih (Sn) pada Hasil Perikanan dengan Inductively Couple Plasma Mass Spectrometer (ICP-MS) secara Simultan. Jakarta.

Chalamaiah M, Kumar BD, Hemalatha R, Jyothirmayi T. 2012. Fish Protein Hydrolysates: Proximate Composition, Amino Acid Composition, Antioxidant Activities and Applications: A Review. Food Chemistry. 135(4): 3020-3038. DOI: https://doi.org/10.1016/j.foodchem.2012.06.100.

Clodoveo ML, Hbaieb RH. 2013. Beyond the Traditional Virgin Olive Oil Extraction Systems: Searching Innovative and Sustainable Plant Engineering Solutions. Food Research International. 54(2): 1926-1933. DOI: https://doi.org/10.1016/j.foodres.2013.06.014.

Cornell JA. 1990. Experiments with Mixtures: Designs, Models, and The Analysis of Mixture Data. New York (US): John Wiley & Sons, Inc.

Darewicz M, Borawska J, Vegarud GE, Minkiewicz P, Iwaniak A. 2014. Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes. International Journal of Molecular Science. 15(8): 14077-14101. DOI: https://doi.org/10.3390/ijms150814077.

Diao Y, Deng A, Wang J, Hong J. 2017. Vitamin A Palmitate Eye Gel Improves the Density of Conjunctival Goblet Cells and the Production of Mucin-5 Subtype AC in Rabbits with Dry Eye Syndrome. International Journal of Clinical and Experimental Medicine. 10(12): 16181-16188.

[EPA] Environmental Protection Agency. 1984. Method 610: Polynuclear Aromatic Hydrocarbons. Washington DC (US): Environmental Protection Agency.

Gamarro EG, Orawattanamateekul W, Sentina J, Gopal TKS. 2013. By-Product of Tuna Processing. Rome (IT): FAO, Globefish.

Hădărugă DI, Ünlüsayin M, Gruia AT, Birău C, Rusu G, Hădărugă NG. 2016. Thermal and Oxidative Stability of Atlantic Salmon Oil (Salmo salar L.) and Complexation with β-cyclodextrin. Beilstein Journal of Organic Chemistry. 12: 179-191. DOI: https://doi.org/10.3762/bjoc.12.20.

Hoyle NT, Merritt JH. 1994. Quality of Fish Protein Hydrolysates from Herring (Clupea harengus). Journal of Food Science. 59(1): 76-79. DOI: https://doi.org/10.1111/j.1365-2621.1994.tb06901.x.

[IUPAC] International Union of Pure and Applied Chemistry. 1987. Standard Methods for the Analysis of Oils, Fats, and Derivatives, 7th. Paquot C dan Hautfenne A (ed). Oxford (GB): Blackwell Scientific.

Jeffrey BG, Weisinger HS, Neuringer M, Mitchell DC. 2001. The Role of Docosahexaenoic Acid in Retinal Function. Lipids. 36(9): 859-871. DOI: https://doi.org/10.1007/s11745-001-0796-3.

[Kemenkes] Kementerian Kesehatan Republik Indonesia. 2016. Infodatin: Pusat Data dan Informasi Kementerian Kesehatan RI: Situasi Balita Pendek. Jakarta.

Koletzko B, Cetin I, Brenna JT. 2007. Dietary Fat Intakes for Pregnant and Lactating Women. British Journal of Nutrition. 98(5): 873-877. DOI: https://doi.org/10.1017/S0007114507764747.

Laemmli UK. 1970. Cleavage of Structural Protein during the Assembly of the Heat of Bacteriophage T4. Nature. 227: 680-685. DOI: http://dx.doi.org/10.1038/227680a0.

Lee JK, Jeon JK, Byun HG. 2015. Characterization of an Antioxidant Peptide from Katsuobushi (Dried Bonito) Protein Hydrolysates. Journal of Marine Bioscience and Biotechnology. 7(1): 19-27. DOI: https://doi.org/10.15433/ksmb.2015.7.1.019.

McClements DJ. 2015. Food Emulsions: Principles, Practices, and Techniques, Third Edition. New York (US): CRC Press.

Nikoo M, Regenstein JM, Yasemi M. 2023. Protein Hydrolysates from Fishery Processing By-Products: Production, Characteristics, Food Applications, and Challenges. Foods. 12(24): 1-28. DOI: https://doi.org/10.3390/foods12244470.

Noman A, Xu Y, Al-Bukhaiti WQ, Abed SM, Ali AH, Ramadhan AH, Xia W. 2017. Influence of Enzymatic Hydrolysis Conditions on the Degree of Hydrolysis and Functional Properties of Protein Hydrolysate Obtained from Chinese Sturgeon (Acipenser sinensis) by Using Papain Enzyme. Process Biochemistry. 67: 19-28. DOI: https://doi.org/10.1016/j.procbio.2018.01.009.

Palacios LE, Wang T. 2005. Egg-yolk Lipid Fractionation and Lecithin Characterization. Journal of the American Oil Chemists’ Society. 82: 571-578. DOI: https://doi.org/10.1007/s11746-005-1111-4.

[Permenkes] Peraturan Menteri Kesehatan Republik Indonesia Nomor 28 Tahun 2019 tentang Angka Kecukupan Gizi yang Dianjurkan untuk Masyarakat Indonesia. Jakarta.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. 1999. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine. 26(9-10): 1231-1237. DOI: https://doi.org/10.1016/S0891-5849(98)00315-3.

Riyanto B, Sinulingga F, Trilaksani W, Fauziah S, Krisnawan WV, Fitradiansyah LO, Rahmat MZ. 2021. Formulasi Pangan Darurat Cookies dengan Pengkayaan Tepung Ikan Lele dan Minyak Ikan Mata Tuna. Jurnal Teknologi Perikanan dan Kelautan. 12(2): 175-181. DOI: https://doi.org/10.24319/jtpk.12.175-181.

Simopoulos AP. 2002. The Importance of the Ratio of Omega-6/Omega-3 Essential Fatty Acid. Biomedicine & Pharmacotherapy. 56(8): 365-379. DOI: https://doi.org/10.1016/S0753-3322(02)00253-6.

Swanson D, Block R, Mousa SA. 2012. Omega-3 Fatty Acids EPA and DHA: Health Benefits Throughout Life. Advances in Nutrition. 3(1): 1-7. DOI: https://doi.org/10.3945/an.111.000893.

Trilaksani W, Riyanto B, Azzahra F, Santoso J, Tarman K. 2020. Recovery of Tuna Virgin Fish Oil and Formulation as a Product Model of Emulsion Food Supplement. The World Seafood Congress 2019 – "Seafood Supply Chains of the Future: Innovation, Responsibility, Sustainability" 9–11 September 2019, Penang, Malaysia. IOP Conference Series: Earth and Environmental Science.

[WHO] World Health Organization. 2012. Nutrition Landscape Information System (NLIS) Country Profile Indicators: Interpretation Guide. Jenewa (CH): WHO.