Lactation traits are controlled by many genes, among others, potentially by growth genes. This research was conducted to study genetic polymorphisms of GH, GHRH, and Pit-1 genes and associations of GH (g.1456_1457 InsT), GHRH (g.4474 C>A), and Pit-1 (g.244 G>A) genotypes with milk yield and quality in Holstein Friesian (HF) cattle. Genotyping was conducted for HF dairy cows raised by small farmers from North Lembang (SF-NL) (98 heads) and South Lembang (SF-SL) (95 heads), and also from Cikole Dairy Cattle Station (CDCS) (82 heads) in Lembang, West Java, Indonesia. Progeny tested of HF bulls (17 heads) from LAIC (Lembang Artificial Insemination Center) in West Java and from SAIC (Singosari AIC) (32 heads) in East Java were also genotyped. Effects of genotypes on test day milk yield, fat content, and SNF content were analyzed by General Linear Models. The GHRH g.4474 C>A SNP and Pit-1 g.244 G>A SNP generated high frequencies of C allele to A allele, while the two allelic frequencies of the GH g.1456_1457 InsT varied. Compared to the AA and AC genotypes, the CC genotype of the GH gene resulted higher test day milk yield (p<0.01), fat content (p<0.05), and SNF content (p<0.05). Further the CC genotype of the GHRH gene yielded higher milk yield (p<0.05), while the GG genotype of the Pit-1 gene resulted higher fat content (p<0.05). Therefore the GH g.1456_1457 InsT, GHRH g.4474 C>A SNP, and Pit-1 g.244 G>A SNP are potential to be used as molecular markers for selection on milk yield and quality in domestic HF cattle.
Ahmadzadeh, M., F. Rashidi, H. A. Najafabadi, A. Jaferian, & S. Eghbalsaied. 2019. Effects of genetic polymorphism in Pit1, GH, GHR and KCN3 on milk yield and body weight of Khuzestan (Iran) water buffaloes. Rev. Colomb. Cienc. Pec. 32:107-116. https://doi.org/10.17533/udea.rccp.v32n2a04
Amiri, S., B. Jemmali, M. Amine Ferchichi, H. Jeljeli, R. Boulbaba, & A. Ben Gara. 2018. Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia. Arch. Anim. Breed. 61:481-489. https://doi.org/10.5194/aab-61-481-2018
Anggraeni, A. 2012. Perbaikan genetik sifat produksi susu dan kualitas susu saoi Friesian Holstein melalui seleksi. Wartazoa 22:1-11.
Anggraeni, A., H. S. Nury, E. Andreas, & C. Sumantri. 2017. Genetic variants of k-Casein and β-Lactoglobulin genes and their association with protein and milk components of Holstein Friesian cows at small farmers in Lembang, West Java. The 2nd International Conference on Sustainable Agriculture and Food Security: A Comprehensive Approach, KnE Life Sciences. pp. 86–94. https://doi.org/10.18502/kls.v2i6.1023
Arango, J., J. J. Echeverri, & A. López. 2014. Association between a polymorphism in intron 3 of the bovine growth hormone gene and growth traits in Holstein heifers in Antioquia. Genet. Mol. Res. 13:6191-6199. https://doi.org/10.4238/2014.August.15.1
Ardicli, S., H. Samli, B. Soyudal, D. Dincel, & F. Balci. 2019. Evaluation of candidate gene effects and environmental factors on reproductive performance of Holstein cows. S. Afr. J. Anim. Sci. 49: 379-294. https://doi.org/10.4314/sajas.v49i2.17
Aytekin, I. & S. Boztepe. 2013. Associations of Pit-1 gene polymorphism with milk yield and composition traits in Brown Swiss cattle. J. Anim. Plant Sci. 23:1281-1289.
Bayram, D., K. Arslan, B. Akyüz, & K. M. Isçan. 2017. Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey. Ankara. Univ. Vet. Fak. Derg. 64:337-343. https://doi.org/10.1501/Vetfak_0000002818
Doosti, A., A. Arshi, & B. Momeni. 2011. Molecular study of PIT1 gene polymorphism in holstein and Iranian native cattle. Afr. J. Agric Res. 6: 4467-4470.
Dybus, A. 2002. Associations of growth hormone (GH) and prolactin (PRL) genes polymorphisms with milk production traits in Polish Black-and-White cattle. Anim Sci. Pap. Rep. 20: 203-212.
Dybus, A., I. Szatkowska, E. Czerniawska-Piatkowska, W. Grzesiak, J. Wójcik, E. Rzewucka, & S. Zych. 2004. PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle. Arch. Anim. Breed. 47: 557-563. https://doi.org/10.5194/aab-47-557-2004
Hartati, H., B. Soewandi, A. Hapsari, S. Anwar, & D. Pamungkas. 2019. Identification of GH|MspI and GHR|AluI gene polymorphism and its association with calf birth weight of Grati-PO cattle. Jurnal Ilmu Ternak dan Veteriner 24: 49-55. https://doi.org/10.14334/jitv.v24i2.1939
Hartatik, T., D. Kurniawati, & Adiarto. 2015. Associations between polymorphism of growth hormone gene with milk production, fat and protein content in Friesian Holstein cattle. J. Indones. Trop. Anim. Agric. 40: 133-137. https://doi.org/10.14710/jitaa.40.3.133-137
Hoseinzadeh, Z. E., M. R. Mohammadabadi, A. Esmailizadeh, A. Khezri, & A. N. Noori. 2015. Association of PIT1 gene with milk fat percentage in Holstein Cattle. J. Livest. Sci. Technol. 3: 41-49.
Huai, Y. T., X. Y. Lan, L. Ma, J. Wang, C. Z. Lei, H. Chen, & J. Q. Wang. 2011. Novel mutation in TGA stop-codon of bovine SIX6 gene. Mol. Biol. 45: 218-224. https://doi.org/10.1134/S0026893310061093
Kiyici, J. M., K. Arslan, B. Akyuz, M. Kaliber, E. G. Aksel, & M. U. Çinar. 2019. Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. Int. J. Dairy Technol. 72: 1-7. https://doi.org/10.1111/1471-0307.12539
Ma, L., Q. Qin, Q. Yang, M. Zhang, H. Zhao, C. Pan, C. Lei, H. Chen, & X. Lan. 2017. Associations of six SNPs of POU1F1-PROP1-PITX1-SIX3 pathway genes with growth traits in two Chinese indigenous goat breeds. Ann. Anim. Sci. 17:399-411. https://doi.org/10.1515/aoas-2016-0066
Özdemir, M. 2012. Determination of Pit-1/Hinf1 polymorphism in Holstein and Native Ear cattle raised as genetic resource in Turkey. J. Anim. Plant Sci. 22: 25-28.
Petrini, J., L. H. S. Iung, M. A. P. Rodriguez, M. Salvian, F. Pértille, G. A. Rovadoscki, L. D. Cassoli, L. L. Coutinho, P. F. Machado, G. R. Wiggans, & G. B. Mourão. 2016. Genetic parameters for milk fatty acids, milk yield and quality traits of a Holstein cattle population reared under tropical conditions. J. Anim. Breed. Genet. 133: 384-395. https://doi.org/10.1111/jbg.12205
Raschia, M. A., J. P. Nani, D. O. Maizon, M. J. Beribe, A. F. Amadio, & M. A. Poli. 2018. Single nucleotide polymorphisms in candidate genes associated with milk yield in argentinean holstein and holstein x jersey cows. J. Anim. Sci. Technol. 60: 1-10. https://doi.org/10.1186/s40781-018-0189-1
Rini, A. O., C. Sumantri, & A. Anggraeni. 2013. GHRH|HaeIII gene polymorphism in dairy and beef cattle at national livestock breeding centers. Med. Pet. 36:185-191. https://doi.org/10.5398/medpet.2013.36.3.185
Sambrook, J., & D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, New York.
Sami, A. J., M. T. Nazir, Z. Jabeen, & A. Shakoori. 2011. Gene study within the 5’ flanking regions of growth hormone gene of first exon in bos indicus. Afr. J. Biotechnol. 10: 332-336.
SAS 9.1. 2011. SQL Procedure User’s Guide. SAS Institute, Cary.
Szatkowska, I., A. Dybus, W. Grzesiak, M. Jędrzejczak, & M. Muszynska. 2009. Association between the growth hormone releasing hormone (Ghrh) gene polymorphism and milk production traits of dairy cattle. J. Appl. Anim. Res. 36: 119-123. https://doi.org/10.1080/09712119.2009.9707044
Yeh, F., & T. Boyle. 1997. Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg. J. Bot. 129:157-63.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors submitting manuscripts should understand and agree that copyright of manuscripts of the article shall be assigned/transferred to Tropical Animal Science Journal. The statement to release the copyright to Tropical Animal Science Journal is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.