Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour
Abstract
Cytochrome P450, family 2, subfamily A, polypeptide 6 (CYP2A6) and kinesin-like protein KIF12 (KIF12) genes are predicted as candidate genes which play important roles in lamb flavour and odour. The aim of this study was to analyse the genotype polymorphism of CYP2A6 and KIF12 genes, to study association and expression of these genes with lamb flavour and odour. Identification of genes polymorphism and associations of CYP2A6 and KIF12 genes were performed using PCR-RFLP method and GLM analysis. The PCR-RFLP products of CYP2A6 and KIF12 were digested by restriction enzyme BsmAI and BfaI, respectively. The expression of CYP2A6 gene was performed using qRT-PCR. The results showed that the CYP2A6 and KIF12 genes were polymorphics. The CYP2A6 gene found to have two genotypes (TT and GT), whereas the KIF12 gene found to have three genotypes (CC, CT, and TT). The CYP2A6 and KIF12 genes were in Hardy Weinberg Equilibrium (HWE). Association analysis showed that CYP2A6 (g.49170107 G>T) was significantly (P<0.05) associated with 3-methylindole (MI) or skatole, while KIF12 (g.9617965 C>T) was not significantly associated with lamb flavour and odour. The GT genotype exhibited a greater 3-methylindole (MI) or skatole than the TT genotype (P<0.05). The mRNA expression analysis showed that CYP2A6 mRNA expression was higher (P<0.01) in animals with the TT genotype. These results will improve the understanding of the functions of the CYP2A6 in lamb flavour and odour, especially in term of 3-methylindole (MI) or skatole compound within the liver and will shed light on CYP2A6 as a candidate in the selection of sheep with low lamb flavour and odour.
References
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Gunawan, A., Jakaria, Listyarini, K., Furqon, A., Sumantri, C., Akter S. H., & Uddin M. J. 2018. Transcriptome signature of liver tissue with divergent sheepmeat odour and flavour using RNA deep sequencing. Gene. 676:86-94.
Hartl, D. L., & Clark, A. G. 1997. Principle of Population Genetic. Sinauer Associates, Sunderland, MA.
Henseler, S., Preuss, S., & Bennewitz, J. 2014. Fleischerzeugung mitMerinolandschaf-Gebrauchskreuzungen – 2. Mitteilung, sensorische Analyse und Zusammenführung der Ergebnisse in einem Index. Züchtungskunde. 86:104-115.
Hirokawa, N., Noda, Y., Tanaka, Y., & Niwa, S. 2009. Kinesin superfamily motor proteins and intracellular transport. Nat Rev Mol Cell Biol. 10:682-696. https://doi.org/10.1038/nrm2774
Hoffman, T. W., Belk, K. E., Woerner, D. R., Tatum, J. D., Delmore, R. J., Peel, R. K., LeValley, S. B., Pendell, D. L., Maneotis, K. A., Zerby, H. N., English, L. F., Moeller, S. J., & Fluharty, F. L. 2015. Executive Summary Preferences and Complaints associated with American Lamb Quality in Retail & Foodservice Markets. Am. Lamb Board. Denver, CO.
Hopkins, D. L., Fogarty, N. M., & Mortimer, S. I. 2011. Genetic related effects on sheep meat quality. Small Ruminant Res. 101:160-72. https://doi.org/10.1016/j.smallrumres.2011.09.036
Jayasena, D. D., Jung, S., Kim, H. J., Alahakoon, A. U., Nam, K. C., & Jo, C. 2014. Effect of Sex on Flavor-related and Functional Compounds in Freeze-dried Broth Made from Korean Native Chicken. Korean J Food Sci Anim Resour. 34:448-456. https://doi.org/10.5851/kosfa.2014.34.4.448
Kayan, A., Cinar, M. U., Uddin, M. J., Phatsara, C., Wimmers, K., Ponsuksili, S., Tesfaye, D., Looft, C., Juengst, H., Tholen, E., & Schellander, K. 2011. Polymorphism and expression of the porcine Tenascin C gene associated with meat and carcass quality. Meat Sci. 89:76-83. https://doi.org/10.1016/j.meatsci.2011.04.001
Lanthier, F., Lou, Y., & Squires, E. J. 2007. Skatole metabolism in the intact pre-pubescent male pig: The relationship between hepatic enzyme activity and skatole concentrations in plasma and fat. Livest Sci. 106:145-153. https://doi.org/10.1016/j.livsci.2006.07.009
Lee, G. J., Archibald, A. L., Law, A. S., Lloyd, S., Wood, J., & Haley, C. S. 2005. Detection of quantitative trait loci for androstenone, skatole and boar taint in a cross between Large White and Meishan pigs. Anim Genet. 36:14-22. https://doi.org/10.1111/j.1365-2052.2004.01214.x
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Matal, J., Mayuskova, Z., Tunkova, A., Anzenbacherova, E., & Anzenbacher, P. 2009. Porcine CYP2A19, CYP2E1, and CYP1A2 Forms are Responsible for Skatole Biotransformation in the Reconstituted System. Neuroendocrinol Lett. 30:36-40.
McRae, J. M., Jaeger, S. R., Bava, C. M., Beresford, M. K., Hunter, D., Jia, Y., Chheang, S. L., Jin, D., Peng, M., Gamble, J. C., Atkinson, K. R., Axten, L. G., Paisley, A. G., William, L., Tooman, L., Pineau, B., Rouse, S. A., & Newcomb, R. D. 2013. Identification of regions associated with variation in sensitivy to food related odors in the human genome. Curr Biol. 23:1596-1600. https://doi.org/10.1016/j.cub.2013.07.031
Moe, M., Lien, S., Aasmundstad, T., Meuwissen, T. H. E., Hansen, M. H. S., Bendixen, C., & Grindflek, E. 2009. Association between SNPs within candidate genes and compounds related to boar taint and reproduction. BMC Genet. 10:32. https://doi.org/10.1186/1471-2156-10-32
Morlein, D., Lungershausen, M., Steinke, K., Sharifi, A. R., & Knorr, C. 2012. A single nucleotide polymorphism in the CYP2E1 gene promoter affects skatole content in backfat of boars of two commercial Duroc-sired crossbred populations. Meat Sci. 92:739-744. https://doi.org/10.1016/j.meatsci.2012.06.031
Nei, & Kumar. 2000. Moleculear Evolution and Phylogenetics. Oxford University Press, New York.
Neuhoff, C., Gunawan, A., Farooq, M. O., Cinar, M. U., Brinkhaus, C. G., Sahadevan, S., Frieden, L., Tesfaye, D., Tholen, E., Looft, C., & et al. 2015. Preliminary study of FMO1, FMO5, CYP21, ESR1, PLIN2, and SULT2A1 as candidate gene for compounds related to boar taint. Meat Sci. 108:67-73. https://doi.org/10.1016/j.meatsci.2015.05.025
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Authors
ListyariniK., JakariaJ., UddinM. J., SumantriC., & GunawanA. (2018). Association and Expression of CYP2A6 and KIF12 Genes Related to Lamb Flavour and Odour. Tropical Animal Science Journal, 41(2), 100-107. https://doi.org/10.5398/tasj.2018.41.2.100
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