Aktivitas Imunomodulator Infusa Ginseng Jawa (Talinum paniculatum) pada Mencit (Mus musculus)
Keywords:
aktivitas fagositosis, ginseng jawa, imunomodulator, indeks fagositosis, makrofag
Abstract
Ginseng jawa diketahui mengandung senyawa aktif yang berpotensi sebagai imunomodulator. Penelitian ini bertujuan mengidentifikasi kemampuan infusa ginseng jawa sebagai imunomodulator berdasarkan aktivitas dan indeks fagositosis makrofag peritoneal mencit serta menentukan dosis yang paling efektif sebagai imunomodulator. Penelitian ini menggunakan 30 ekor mencit galur DDY jantan yang dibagi dalam 5 kelompok, yaitu kontrol negatif (air mineral), kontrol positif (sediaan imunomodulator komersial), infusa ginseng jawa (IGJ) dosis 33 mg/kg BB, 66 mg/kg BB, dan 200 mg/kg BB. Pemberian infusa ginseng jawa dilakukan satu kali sehari selama 14 hari secara peroral dengan mikropipet. Mencit diinduksi dengan Staphylococcus aureus nonpatogen (108 CFU/ml) pada hari ke-15 secara intraperitoneal sebelum dilakukan koleksi cairan peritoneal. Cairan peritoneal lalu dibuat preparat ulas dan dilakukan pengamatan terhadap jumlah makrofag aktif dan S. aureus yang terfagositosis. Selanjutnya aktivitas fagositosis dan indeks fagositosis makrofag dihitung. Hasil pengujian menunjukkan aktivitas fagositosis dan indeks fagositosis pada seluruh kelompok yang diberikan infusa ginseng jawa berbeda nyata (p<0,05) dibandingkan dengan kelompok kontrol. Aktivitas dan indeks fagositosis tertinggi ditemukan pada kelompok IGJ 200 mg/kg BB dengan nilai berturut-turut 74,83%±2,32% dan 3,07±0,05. Infusa ginseng jawa memiliki kemampuan sebagai imunomodulator dengan meningkatkan respons imun nonspesifik berupa aktivitas dan indeks fagositosis makrofag.
References
[1] Artini, K. & Veranita, W. (2021). Tanaman herbal untuk meningkatkan sistem imun tubuh: literature review. Jurnal Farmasetis, 10(1), 15-20.
[2] Martinus, Agustin, T., Dachlan, A. & Effendi, E. (2019) Penggunaan imunostimulan dalam bidang dermatovenereologi. Majalah Dermato-Venereologica Indonesiana, 46(2), 111-115.
[3] Devagaran, T. & Diantini, A. (2012). Senyawa imunomodulator dari tanaman. Students e-Journal, 1(1), 1-2.
[4] Yassir, M. & Asnah. (2018). Pemanfaatan jenis tumbuhan obat tradisional di Desa Batu Hamparan. Jurnal Biotik, 6(1), 17-34.
[5] Wasito. (2018). Peran perguruan tinggi farmasi dalam pengembangan industri kecil obat tradisional untuk pengentasan kemiskinan. Wawasan Tri Dharma Majalah Ilmiah Kopertis, 4(8), 17-34.
[6] Syukur, C. & Hernani. (2003). Budidaya Tanaman Obat Komersial. Jakarta: Penyebar Swadaya.
[7] Silalahi, M. (2022). Talinum paniculatum (Jacq.) Gaertn (kajian pemanfaatannya sebagai bahan pangan dan bioaktivitasnya). Pro-Life, 9(1), 289-299.
[8] Syamsuhidayat, S. & Hutapea, J. (1991). Inventaris Tanaman Obat Indonesia. Jakarta: Litbang Kesehatan Departemen RI.
[9] Sulistiono, Kristianti, A. & Santoso, A. (2017). Talinum paniculatum (Jacq) Gaertn (Java ginseng) production using vesicular-arbuscular mycorrhizal. International Journal of Applied Biology, 1(2), 76-81.
[10] Yong-Chin, L., Jiann-Chu, C., Wan, Z., Putra, D., Chien-Lun, H., Chang-Che, L. & Jen-Fang, H. (2013). Vaccination enhance early immune responses in white shrimp Litopenaeus vannamei after secondary exposure to Vibrio alginolyticus. PLoS One, 8(7), e69722.
[11] Rosnizar, R., Nanza, S. & Eriani, N. (2021). Uji efektivitas ekstrak rimpang alang-alang (Imperata cylindria (L.) P. Beauv) sebagai bahan imunostimulator. Bioleuser, 5(2), 17-21.
[12] Gordon, S. & Taylor, P. (2005). Monocyte and macrophage heterogeneity. Nature Reviews Immunology, 5(12), 953-964.
[13] Ginhoux, F. & Jung, S. (2014). Monocytes and macrophages: developmental pathways and tissue homeostasis. Nature Reviews Immunology, 14(6), 392-404.
[14] Gonçalves, R. & Mosser, D. (2015). The isolation and characterization of murine macrophage. Current Protocol Immunology, 111, 14.1.1-14.1.16.
[15] Cassado, A., Lima, M. & Bortoluci, K. (2015). Revisiting mouse peritoneal macrophages: heterogeneity, development, and function. Frontiers in Immunology, 6, 225.
[16] Baratawijaya, K. (1991). Imunologi Dasar, Edisi ke-2. Jakarta: FKUI.
[17] Ray, A. & Dittel, B. (2010). Isolation of mouse peritoneal cavity cells. Journal of Visualized Experiments, 35, e1488.
[18] Zhang, X., Gonçalves, R. & Mosser, D. (2008). The isolation and characterization of murine macrophages. Current Protocol Immunology, 14, 14.1.1-14.1.14.
[19] Abbas, A., Lichtman, A. & Pillai, S. (2017). Cellular and Molecular Immunology, 9th ed. Philadelphia: Elsevier.
[20] Marshall, J., Warrington, R., Watson, W. & Kim, H. (2018). An introduction to immunology and immunopathology. Allergy, Asthma & Clinical Immunology, 14(2), 49.
[21] Sriningsih & Wibawa, A. (2006). Efek protektif pemberian ekstrak etanol herba meniran (Phyllantus niruri L) terhadap aktivitas dan kapasitas fagositosis makrofag peritoneum tikus. Artocarpus, 6, 91-96.
[22] Isaza-Restrepo, A., Martin-Saavedra, J., Velez-Leal, J., Vargas-Barato, F. & Riveros, D. (2018). The peritoneum: beyond the tissue - a review. Frontiers in Physiology, 9, 738.
[23] Lachaud, C., Rodriguez-Campins, B., Hmadcha, A. & Soria, B. (2015). Use of mesothelial cells and biological matrices for tissue engineering of simple epithelium surrogates. Frontiers in Bioengineering and Biotechnology, 3, 117.
[24] Fournier, B. & Philpott, D. (2005). Recognition of Staphylococcus aureus by the innate immune system. Clinical Microbiology Reviews, 18(3), 521-540.
[25] Pidwill, G., Gibson, J., Cole, J., Renshaw, S. & Foster, S. (2021). The role of macrophages in Staphylococcus aureus infection. Frontiers in Immunology, 11, e620339.
[26] Rodriguez, L. & Le Moullac, G. (2000). State of the art of immunological tools and health control of penaeid shrimp. Aquaculture, 191(1-3), 109-119.
[27] Vyas, J., Van der Veen, A. & Ploegh, H. (2008). The known unknowns of antigen processing and presentation. Nature Reviews Immunology, 8(8), 607-618.
[28] Guilliams, M., Ginhoux, F., Jakubzick, C., Naik, S., Onai, N., Schraml, B., Segura, E., Tussiwand, R. & Yona, S. (2014). Dendritic cells, monocytes, and macrophage: a unified nomenclature based on ontogeny. Nature Reviews Immunology, 14(8), 571-578.
[29] Sartini & Usman, M. (2014). Uji antimikroba ekstrak akar som jawa (Talinum paniculatum). Biolink, 1(1), 1-9.
[30] Riyana, A., Mudigdo, A. & Wasita, B. (2019). The effects of ginseng java roots (Talinum paniculatum) extract on malondialdehyde (MDA) levels in male white sprague dawley rats with forced swimming test model. IOP Conference Series, 546(6), e062025.
[31] Liao, D., Chai, Y., Wang, S., Chen, C. & Tsai, M. (2015). Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects. Journal of Food and Drug Analysis, 23(2), 294-302.
[32] Jayaraman, P., Ovalle-Sada, I., Nishimura, T., Anderson, A., Kuchroo, V., Remold, H. & Behar, S., (2013). IL-1ß promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. The Journal of Immunology, 190(8), 4196-4204.
[33] Wu, C., Xue, Y., Wang, P., Lin, L., Liu, Q., Li, N., Xu, J. & Cao, X. (2014). IFN-γ primes macrophage activation by increasing phosphatase and tensin homolog via downregulation of miR-3473b. The Journal of Immunology, 193(6), 3036-3044.
[34] Parameswaran, N. & Patial, S. (2010). Tumor necrosis factor-α signaling in macrophages. Critical Reviews in Eukaryotic Gene Expression, 20(2), 87-103.
[35] Salim, T., Sershen, C. & May, E. (2016). Investigating the role of TNF-α and IFN-γ activation on the dynamics of iNOS gene expression in LPS stimulated macrophages. PLoS One, 11(6), e0153289.
[36] Chahal, K. & Jha, M. (2020). In vivo study of Boswellia serrata for modulating immune system and quenching free radicals. Advanced in Zoology and Botany, 8(4), 358-368.
[37] O'Shea, J., Gadina, M. & Siegel, R. (2019). Cytokines and cytokine receptors. Di dalam: Rich, R.R., Fleisher, T.A., Shearer, W.T., Schroeder, H.W., Frew, A.J., Weyand, C.M., editor. Clinical Immunology Principles and Practice, 5th ed., Philadelphia: Elsevier.
[38] Arayan, L., Simborio, H., Reyes, A., Hop, H., Min, W., Lee, H., Rhee, M., Chang, H. & Kim, S. (2015). The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells. FEMS Microbiology Letters, 362(11), fnv070.
[39] Jie, Y., Cammisuli, S. & Baggliolini, M. (1984). Immunomodulatory effects of Panax ginseng CA meyer in the mouse. Agents Actions, 15(3-4), 386-391.
[2] Martinus, Agustin, T., Dachlan, A. & Effendi, E. (2019) Penggunaan imunostimulan dalam bidang dermatovenereologi. Majalah Dermato-Venereologica Indonesiana, 46(2), 111-115.
[3] Devagaran, T. & Diantini, A. (2012). Senyawa imunomodulator dari tanaman. Students e-Journal, 1(1), 1-2.
[4] Yassir, M. & Asnah. (2018). Pemanfaatan jenis tumbuhan obat tradisional di Desa Batu Hamparan. Jurnal Biotik, 6(1), 17-34.
[5] Wasito. (2018). Peran perguruan tinggi farmasi dalam pengembangan industri kecil obat tradisional untuk pengentasan kemiskinan. Wawasan Tri Dharma Majalah Ilmiah Kopertis, 4(8), 17-34.
[6] Syukur, C. & Hernani. (2003). Budidaya Tanaman Obat Komersial. Jakarta: Penyebar Swadaya.
[7] Silalahi, M. (2022). Talinum paniculatum (Jacq.) Gaertn (kajian pemanfaatannya sebagai bahan pangan dan bioaktivitasnya). Pro-Life, 9(1), 289-299.
[8] Syamsuhidayat, S. & Hutapea, J. (1991). Inventaris Tanaman Obat Indonesia. Jakarta: Litbang Kesehatan Departemen RI.
[9] Sulistiono, Kristianti, A. & Santoso, A. (2017). Talinum paniculatum (Jacq) Gaertn (Java ginseng) production using vesicular-arbuscular mycorrhizal. International Journal of Applied Biology, 1(2), 76-81.
[10] Yong-Chin, L., Jiann-Chu, C., Wan, Z., Putra, D., Chien-Lun, H., Chang-Che, L. & Jen-Fang, H. (2013). Vaccination enhance early immune responses in white shrimp Litopenaeus vannamei after secondary exposure to Vibrio alginolyticus. PLoS One, 8(7), e69722.
[11] Rosnizar, R., Nanza, S. & Eriani, N. (2021). Uji efektivitas ekstrak rimpang alang-alang (Imperata cylindria (L.) P. Beauv) sebagai bahan imunostimulator. Bioleuser, 5(2), 17-21.
[12] Gordon, S. & Taylor, P. (2005). Monocyte and macrophage heterogeneity. Nature Reviews Immunology, 5(12), 953-964.
[13] Ginhoux, F. & Jung, S. (2014). Monocytes and macrophages: developmental pathways and tissue homeostasis. Nature Reviews Immunology, 14(6), 392-404.
[14] Gonçalves, R. & Mosser, D. (2015). The isolation and characterization of murine macrophage. Current Protocol Immunology, 111, 14.1.1-14.1.16.
[15] Cassado, A., Lima, M. & Bortoluci, K. (2015). Revisiting mouse peritoneal macrophages: heterogeneity, development, and function. Frontiers in Immunology, 6, 225.
[16] Baratawijaya, K. (1991). Imunologi Dasar, Edisi ke-2. Jakarta: FKUI.
[17] Ray, A. & Dittel, B. (2010). Isolation of mouse peritoneal cavity cells. Journal of Visualized Experiments, 35, e1488.
[18] Zhang, X., Gonçalves, R. & Mosser, D. (2008). The isolation and characterization of murine macrophages. Current Protocol Immunology, 14, 14.1.1-14.1.14.
[19] Abbas, A., Lichtman, A. & Pillai, S. (2017). Cellular and Molecular Immunology, 9th ed. Philadelphia: Elsevier.
[20] Marshall, J., Warrington, R., Watson, W. & Kim, H. (2018). An introduction to immunology and immunopathology. Allergy, Asthma & Clinical Immunology, 14(2), 49.
[21] Sriningsih & Wibawa, A. (2006). Efek protektif pemberian ekstrak etanol herba meniran (Phyllantus niruri L) terhadap aktivitas dan kapasitas fagositosis makrofag peritoneum tikus. Artocarpus, 6, 91-96.
[22] Isaza-Restrepo, A., Martin-Saavedra, J., Velez-Leal, J., Vargas-Barato, F. & Riveros, D. (2018). The peritoneum: beyond the tissue - a review. Frontiers in Physiology, 9, 738.
[23] Lachaud, C., Rodriguez-Campins, B., Hmadcha, A. & Soria, B. (2015). Use of mesothelial cells and biological matrices for tissue engineering of simple epithelium surrogates. Frontiers in Bioengineering and Biotechnology, 3, 117.
[24] Fournier, B. & Philpott, D. (2005). Recognition of Staphylococcus aureus by the innate immune system. Clinical Microbiology Reviews, 18(3), 521-540.
[25] Pidwill, G., Gibson, J., Cole, J., Renshaw, S. & Foster, S. (2021). The role of macrophages in Staphylococcus aureus infection. Frontiers in Immunology, 11, e620339.
[26] Rodriguez, L. & Le Moullac, G. (2000). State of the art of immunological tools and health control of penaeid shrimp. Aquaculture, 191(1-3), 109-119.
[27] Vyas, J., Van der Veen, A. & Ploegh, H. (2008). The known unknowns of antigen processing and presentation. Nature Reviews Immunology, 8(8), 607-618.
[28] Guilliams, M., Ginhoux, F., Jakubzick, C., Naik, S., Onai, N., Schraml, B., Segura, E., Tussiwand, R. & Yona, S. (2014). Dendritic cells, monocytes, and macrophage: a unified nomenclature based on ontogeny. Nature Reviews Immunology, 14(8), 571-578.
[29] Sartini & Usman, M. (2014). Uji antimikroba ekstrak akar som jawa (Talinum paniculatum). Biolink, 1(1), 1-9.
[30] Riyana, A., Mudigdo, A. & Wasita, B. (2019). The effects of ginseng java roots (Talinum paniculatum) extract on malondialdehyde (MDA) levels in male white sprague dawley rats with forced swimming test model. IOP Conference Series, 546(6), e062025.
[31] Liao, D., Chai, Y., Wang, S., Chen, C. & Tsai, M. (2015). Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects. Journal of Food and Drug Analysis, 23(2), 294-302.
[32] Jayaraman, P., Ovalle-Sada, I., Nishimura, T., Anderson, A., Kuchroo, V., Remold, H. & Behar, S., (2013). IL-1ß promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. The Journal of Immunology, 190(8), 4196-4204.
[33] Wu, C., Xue, Y., Wang, P., Lin, L., Liu, Q., Li, N., Xu, J. & Cao, X. (2014). IFN-γ primes macrophage activation by increasing phosphatase and tensin homolog via downregulation of miR-3473b. The Journal of Immunology, 193(6), 3036-3044.
[34] Parameswaran, N. & Patial, S. (2010). Tumor necrosis factor-α signaling in macrophages. Critical Reviews in Eukaryotic Gene Expression, 20(2), 87-103.
[35] Salim, T., Sershen, C. & May, E. (2016). Investigating the role of TNF-α and IFN-γ activation on the dynamics of iNOS gene expression in LPS stimulated macrophages. PLoS One, 11(6), e0153289.
[36] Chahal, K. & Jha, M. (2020). In vivo study of Boswellia serrata for modulating immune system and quenching free radicals. Advanced in Zoology and Botany, 8(4), 358-368.
[37] O'Shea, J., Gadina, M. & Siegel, R. (2019). Cytokines and cytokine receptors. Di dalam: Rich, R.R., Fleisher, T.A., Shearer, W.T., Schroeder, H.W., Frew, A.J., Weyand, C.M., editor. Clinical Immunology Principles and Practice, 5th ed., Philadelphia: Elsevier.
[38] Arayan, L., Simborio, H., Reyes, A., Hop, H., Min, W., Lee, H., Rhee, M., Chang, H. & Kim, S. (2015). The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells. FEMS Microbiology Letters, 362(11), fnv070.
[39] Jie, Y., Cammisuli, S. & Baggliolini, M. (1984). Immunomodulatory effects of Panax ginseng CA meyer in the mouse. Agents Actions, 15(3-4), 386-391.
Published
2024-03-28
How to Cite
DigyoO. D. K., Safika, & Andriyanto. (2024). Aktivitas Imunomodulator Infusa Ginseng Jawa (Talinum paniculatum) pada Mencit (Mus musculus). Jurnal Veteriner Dan Biomedis, 2(1), 1-8. https://doi.org/10.29244/jvetbiomed.2.1.1-8.
Section
PENELITIAN/ RESEARCH
Copyright (c) 2024 Oscar Daniel Kusumo Digyo, Safika, Andriyanto
This work is licensed under a Creative Commons Attribution 4.0 International License.
