Optimasi Level Benzyl Amino Purin (BAP) terhadap Pertumbuhan Tanaman Kembang Telang (Clitoria ternatea) melalui Teknik Kultur Jaringan

Optimization of Benzyl AminoPurin (BAP) Levels for the Growth of Butterfly Pea (Clitoria ternatea) Plants through Tissue Culture Techniques

  • K P A Jannah Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, IPB University
  • I Prihantoro Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, IPB University
  • P D M H Karti Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, IPB University
Keywords: BAP (Benzyl Amino Purin), kembang telang, kultur jaringan

Abstract

The research objective was to measure the optimum level of BAP for supporting the growth of butterfly pea flowers through tissue culture techniques. The study used a completely randomized design (CRD) with 5 treatments based on BAP media levels (BAP 0 ppm, BAP 0.5 ppm, BAP 1 ppm, BAP 1.5 ppm, and BAP 2 ppm) and 20 replications. Parameters measured were plant height, plant height increase, number of leaves, number of branches, number of tillers, percentage of tiller growth, plant weight, and leaf color. The results showed that the use of BAP levels 0, 0.5, 1, 1.5, and 2 ppm in butterfly pea flower plants through tissue culture techniques was not effective in increasing plant height, number of leaves, number of branches, number of tillers, and percentage of tillers at the end of the research (27 DAP). Using an optimum BAP level of 0.5 ppm resulted in higher biomass production and the dominance of green leaf color visualization.

Key words:        BAP (Benzyl Amino Purine), butterfly pea, Clitoria ternatea, tissue culture

Downloads

Download data is not yet available.

Author Biography

P D M H Karti, Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, IPB University

Department of Nutrition Science and Feed Technology, Faculty of Animal Science.

References

Adip MS, Hendrarto B & Purwanti B. 2014. Nilai hue daun rhizospora: Hubungannya dengan faktor lingkungan dan klorofil daun di pantai ringgung, Desa Sidodai, Kecamatan Padang Cermin, Lampung. Maquares. 3(2): 20-26. doi: 10.14710/marj.v3i2.4839

Ai NS, Rumbay JA, Anggini PS, Supit PSL & Ludong DPM. 2021. Potensi metode sonic bloom untuk meningkatkan pertumbuhan tanaman. Jurnal MIPA.10(2): 76-80.

Arafah DL, Hernawati D & Nuryadin E. 2021. The effect hormone BAP (6-benzyl amino purine) on the growth of potato axilarry shoots (Solanum tuberosum L.) in vitro. Jurnal Biologi Tropis. 21(3): 641-647. doi: 10.29303/jbt.v21i3.2823

Azwin. 2016. Penggunaan BAP dan TDZ untuk perbanyakan tanaman gaharu (Aquilaria malaccensis Lamk.). Jurnal Ilmiah Pertanian 13(1): 59-69.

Dharmadewi AAIM. 2020. Analisis kandungan klorofil pada beberapa jenis sayuran hijau sebagai alternatif bahan dasar food suplement. Jurnal Edukasi Matematika dan Sains. 9(2): 171-177. doi: 10.5281/zenodo.4299383

Jelantik IGB, Nikolaus TT, Penu CL, Malelak GEM & Benu I. 2019. Produksi dan kualitas hijauan kacang kupu (Clitoria ternatea) yang dipanen pada umur 60. 75, dan 90 hari. Pastura. 8(2): 76-80

Kartiman R, Sukma D, Aisyah SI & Purwito A. 2018. Multiplikasi in vitro anggrek hitam (Coelogyne pandurata Lindl.) pada perlakuan kombinasi NAA dan BAP. Jurnal Bioteknologi dan Biosains Indonesia 5 (1) : 75 – 87. doi:10.18311/jnr/2015/480

Lestari FW, Suminar E & Mubarok S. 2018. Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi BAP dan NAA yang berbeda. Jurnal Agro. 5(1): 66–75. doi: 10.15575/1348

Mashud. 2013. Efek zat pengatur tumbuh BAP terhadap pertumbuhan plantlet genjah kopyor dari kecambah yang dibelah. B. Palma. 14 (2): 82-87.

Mawaddah Y, Erawati DN, Danianto M, Ryana WM & Ikanafi’ah A. 2021. Peran sitokinin terhadap penggandaan tunas eksplan vanili (Vanilla planifolia Andrews.). Journal of Applied Agricultural Science. 5(2): 169-179. doi: 10.25047/agriprima.v5i2.441

Muliawati .2017. Aklimatisasi Planlet Pisang Varietas Raja Bulu Kuning Berbasis Sistem Hidroponik Substrat. Agrotechnology Research Journal 1(2): 1–6. doi: 10.20961/agrotechresj.v1i2.18876

Nofiyanto RT, Kusmiyati F & Karno. 2019. Peningkatan kualitas planlet tanaman pisang raja bulu (Musa paradisiaca) dengan penambahan bap dan iaa pada media pengakaran kultur in vitro. Journal of Agro Complex. 3(3): 132-141. doi: 10.14710/joac.3.3.132-141

Nuraini A, Aprilia E, Murgayanti & Wulandari AP. 2022. Pengaruh konsentrasi Benzylaminopurine terhadap pertumbuhan eksplan tunas aksilar rami klon lokal Wonosobo secara in vitro. Jurnal Kultivasi. 21(2): 166-172.

Oguis GK, Gilding EK, Jackson MA & Craik DJ. 2019. Butterflly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Frontiers in Plant Science. 10 : 645

Panjaitan E. 2005. Renspon pertumbuhan tanaman anggrek (Dendrobium sp.) terhadap Pemberian BAP dan NAA secara in vitro. Jurnal Penelitian Bidang Ilmu Pertanian. 3(3): 45-51.

Pierik RLM. 1997. In Vitro Culture of Higher Plants. Dordrecht (NL): Kluwer Academic Publishers.

Purita SY, Ardiarini NR & Basuki N. 2017. Pengatur tumbuh jenis bap terhadap pertumbuhan planlet sub kultur jaringan tanaman nanas (Ananas comosus L. Merr). Jurnal Produksi Tanaman. 5(7):1207 – 1212.

Ratnasari BD, Suminar E, Nuraini A & Ismail. 2016. Pengujian efektivitas berbagai jenis dan konsentrasi sitokinin terhadap multiplikasi tunas mikro pisanh (Musa paradisiaca L.) secara in vitro. Kultivasi. 15(2): 74-80.

Reddy DRD, Suvarna D & Rao DM. 2014. Effects of 6-benzyl amino purine (6-BAP) on in vitro shoot multiplication of grand naine (Musa sp.). International Journal of Advanced Biotechnology Research 5(1): 36-42.

Sagai E, Doodoh B & Kojoh D. 2016. Pengaruh zat pengatur tumbuh benzil amino purin (bap) terhadap induksi dan multiplikasi tunas brokoli (Brassica oleraceae L.). Jurnal Sam Ratulangi. 7(6): 1-10. doi: 10.35791/cocos.v7i6.13885

Sitanggang A, Islan & Saputra SI. 2015. Pengaruh pemberian pupuk kandang ayam dan zat pengatur tumbuh giberelin terhadap pertumbuhan bibit kopi arabika (Coffea arabica L.). Jurnal Online Mahasiswa Faperta UNRI. 2(1): 1-12.

Sofian AA, Prihastanti E, Widodo S & Suedy A. 2018. Effect of IBA and BAP on shoot growth of tawangmangu tangerine (Citrus reticulate) by in-vitro. Biosaintifika. 10(2): 379–387.

Sulaiman S, Yusuf MA & Awal A. 2020. Effect of plant growth regulators on in vitro culture of pineapple (Ananas comosis L. Merr) MID2 variety. Food Research. 4(4): 110–114.

Sutedi E. 2013. Potensi kembang telang (Clitoria ternatea) sebagai tanaman pakan ternak. Wartazoa. 23(2): 51-62.

Syamsiah M, Imansyah AA, Suprapti HK & Badriah DS. 2020. Respon multiplikasi anggrek bulan (Phalaenopsis sp.) terhadap penambahan beberapa konsentrasi BAP (Benzyl Amino Purine) pada media in vitro. Agroscience. 10(2): 148-159.

Talla SK, Panigrahy M, Kappara S, Nirosha P, Neelamraju S & Ramanan R. 2016. Cytokinin delays dark induces senescence in rice by maintaining the chlorophyll cycle and photosynthetic complexes. Journal of Experimental Botany. 67(6):1839–1851. doi:10.1093/jxb/erv575

Tilaar W, Rantung J & Tulung S. 2015. Shoot induction from nodul segments of the kulo ,Chrysanthemun variety in cytokines enhanced murashige skoog growth media. Jurnal Eugenia. 21(1): 94-104.

Wilde SA. 1952. Munsell Color Chart for Plant Tissue. New York(US): The Munsell Color Company.

Ziraluo YPB. 2021. Metode perbanyakan tanaman ubi jalar ungu (Pomea batatas poiret) dengan teknik kultur jaringan atau stek planlet. Jurnal Inovasi Penelitian. 2(3): 1037-1046.

Published
2023-08-31
How to Cite
JannahK. P. A., PrihantoroI., & karti panca dewi manu hara. (2023). Optimasi Level Benzyl Amino Purin (BAP) terhadap Pertumbuhan Tanaman Kembang Telang (Clitoria ternatea) melalui Teknik Kultur Jaringan: Optimization of Benzyl AminoPurin (BAP) Levels for the Growth of Butterfly Pea (Clitoria ternatea) Plants through Tissue Culture Techniques. Jurnal Ilmu Nutrisi Dan Teknologi Pakan, 21(2), 100-106. https://doi.org/10.29244/jintp.21.2.100-106