Pengaruh Penambahan Nanoselulosa terhadap Stabilitas Emulsi Santan sebagai Enkapsulan Kurkumin: Stabilitas Emulsi dan Kurkumin
Abstract
Curcumin is a bioactive compound characterized by its non-polar nature and low stability. Encapsula-tion of curcumin using emulsion system such as coconut milk can increase its stability. However, emulsion systems tend to become unstable during food processing and storage. Therefore, stabilizers and emulsifiers are necessary to ensure stability. Natural stabilizers are preferred over synthetic ones. In this study, nano-celluloses, including nanocrystalline and nanofibrillated cellulose, were used as a stabilizer, and whey pro-tein isolate (WPI) was used as an emulsifier. The primary objective of this study was to investigate the impact of different types and concentrations of nanocellulose on the stability of curcumin and oil-in-water (o/w) emulsions in coconut milk. Two types of commercial nanocellulose materials were utilized: nanocrystalline cellulose (NCC) and nanofibrillated cellulose (NFC). Additionally, a combination of both NCC and NFC was used at concentrations of 0.125 and 0.25% (w/w). The results revealed that the viscosity increased as the concentration of nanocellulose increased. The particle size increased at higher concentrations of NFC and the combination of NCC and NFC, while it decreased with increasing NCC concentration. The lightness va-lue (L*) decreased with higher concentrations of nanocellulose in all sample types. However, this difference was not visually significant. In terms of curcumin stability, the addition of nanocellulose, particularly NFC at a concentration of 0.25%, led to an increase in stability. Higher viscosity resulted in an extended shelf life because it slowed down the rate of creaming index as compared to the control emulsion. Increasing the concentration of nanocellulose enhanced the effectiveness of maintaining the stability of curcumin and oil-in-water emulsions in coconut milk due to the higher viscosity. NFC as well as the combination of NFC and NCC at a concentration of 0.25% proved to be the most preferable option for achieving the highest stability.
References
Anisuzzaman SM, Bono A, Krishnaiah D, Hussin NA. 2014. Decolorization of low molecular compounds of seaweed by using activated carbon. Int J Chem Eng Appl 5: 100–103. https://doi.org/010.7763/IJCEA.2014.V5.359
Bhat AH, Dasan YK, Khan I, Soleimani H, Usmani A. 2017. Application of nanocrystalline cellulose: Processing and biomedical applications. Cellulose-Reinforced Nanofibre Composites: Production, Properties and Applications 5: 215–240. https://doi.org/10.1016/B978-0-08-100957-4.00009-7
Capitani MI, Nolasco SM, Tomás MC. 2016. Stability of oil-in-water (O/W) emulsions with chia (Salvia hispanica L.) mucilage. Food Hydrocolloids 61: 537-546. https://doi.org/10.1016/j.foodhyd.2016.06.008
Carrillo CA, Nypelö TE, Rojas OJ. 2015. Cellulose nanofibrils for one-step stabilization of multiple emulsions (W/O/W) based on soybean oil. J Colloid Interface Sci 445: 166–173. https://doi.org/10.1016/j.jcis.2014.12.028
Cookson MD, Stirk PMR. 2015. Food Emulsions. Principles, Practices and Techinques. 211-212. CRC Press, Boca Raton.
Degner BM, Chung C, Schlegel V, Hutkins R, Mcclements DJ. 2014. Factors influencing the freeze-thaw stability of emulsion-based foods. Compr Rev Food Sci Food Saf 13: 98–113. https://doi.org/10.1111/1541-4337.12050
Fan X, Zhang C, LiU D, Yan J, Liang H. 2013. The Clinical applications of curcumin: Current state and the future. Curr Pharm Design 19: 2011–2031. https://doi.org/10.2174/1381612811319110005
Fitri IA, Winuprasith T. 2022. Stability β-carotene encapsulated in plant-based emulsions: Impact of nanocrystalline cellulose concentration. Biosci 6: 1–11. https://doi.org/10.24036/0202261116540-0-00
Hayati HR, Dewi AK, Nugrahani RA, Satibi L. 2015. Pengaruh konsentrasi maltodekstrin terhadap kadar air dan waktu melarutnya santan kelapa bubuk (coconut milk powder) dalam air. J Teknol 7: 55–60.
Isogai A. 2013. Wood nanocelluloses: Fundamentals and applications as new bio-based nanomaterials. J Wood Sci 59: 449–459. https://doi.org/10.1007/s10086-013-1365-z
Jiang F, Hsieh Y-L. 2013. Chemically and mechanically isolated nanocellulose and their self-assembled structures. Carbohydr Polym 95: 32–40. https://doi.org/10.1016/j.carbpol.2013.02.022
Keowmaneechai E, McClements DJ. 2002. Influence of EDTA and citrate on physicochemical proper-ties of whey protein-stabilized oil-in-water emulsions containing CaCl2. J Agric Food Chem 50: 7145–7153. https://doi.org/10.1021/jf020489a
Lawrence M, Jayne, Rees G D. 2000. Microemul-sions-based media asnnovel drug delivery systems. Adv Drug Deliv Rev 45: 89-121. https://doi.org/10.1016/S0169-409X(00)00103-4
Li J, Xu X, Chen Z, Wang T, Lu Z, Hu W, Wang L. 2018. Zein/gum Arabic nanoparticle-stabilized Pickering emulsion with thymol as an anti-bacterial delivery system. Carbohydr Polym 200: 416–426. https://doi.org/10.1016/j.carbpol.2018.08.025
Liu W, Wang J, McClements DJ, Zou L. 2018 Encap-sulation of β-carotene-loaded oil droplets in caseinate/alginate microparticles: Enhancement of carotenoid stability and bioaccessibility. J Funct Foods 40: 527–535. https://doi.org/10.1016/j.jff.2017.11.046
Lu P, Hsieh Y-L. 2012. Preparation and characteriza-tion of cellulose nanocrystals from rice straw. Carbohydr Polym 87: 564–573. https://doi.org/10.1016/j.carbpol.2011.08.022
McClements DJ. 2015. Enhancing nutraceutical bio-availability through food matrix design. Curr Opinion Food Sci 4: 1–6. https://doi.org/10.1016/j.cofs.2014.12.008
Mitbumrung W, Jain S, Winuprasith T. 2020. Properties and stability of pickering emulsions stabilized by nanofibrillated mangosteen cellulose: Impact of oil type and emulsifier concentration. Songklanakarin J Sci Technol 42: 468–476.
Mitbumrung W, Rungraung N, Muangpracha, N, Akanitkul P, Winuprasith T. 2022. Approaches for extracting nanofibrillated cellulose from oat bran and its emulsion capacity and stability. Polymers 14: 1-7. https://doi.org/10.3390/polym14020327
Ni X, Chen W, Xiao M, Wu K, Kuang Y, Corke H, Jiang F. 2016. Physical stability and rheological properties of konjac glucomannan-ethyl cellulose mixed emulsions. Int J Biological Macromol 92: 423–430. https://doi.org/10.1016/j.ijbiomac.2016.07.018
Norizan MN, Shazleen SS, Alias AH, Sabaruddin FA, Asyraf MRM, Zainudin ES, Abdullah N, Samsudin MS, Kamarudin SH, Norrrahim MNF. 2022. Nanocellulose-based nanocomposite for sustainable application: review. Nanomaterials 12: 3483. https://doi.org/10.3390/nano12193483
Ostbring K, Matos M, Marefati A, Ahlstrom C, Gutierrez G. 2015. The effect of pH and storage temperature on the stability of emulsions stabilized by rapeseed protein. Foods 10: 1657. https://doi.org/10.3390/foods10071657
Parthasarathy VA, Chempakam B. Zachariah TJ. 2008. Chemistry of Spices. 1-20. CABI Publishing, New York. https://doi.org/10.1079/97818 45934057.0000
Rayner M, Sjöö M, Timgren A, Dejmek P. 2012. Quinoa starch granules as stabilizing particles for production of Pickering emulsions. Faraday Discuss 158: 139–155. https://doi.org/10.1039/c2fd20038d
Suseno SH, Jacoeb AM, Nuryanti M, Ernawati. 2017. Sardine (Sardinella sp.) oil emulsion and its stability during storage. World J Fish Marine Sci 9: 31-38.
Tangsuphoom N, Coupland J. 2009. Effect of thermal treatments on the properties of coconut milk emulsions prepared with surface-active stabilizers. Food Hydrocolloids 23: 1792-1800. https://doi.org/10.1016/j.foodhyd.2008.12.001
Varanasi S, He R, Batchelor W. 2013. Estimation of cellulose nanofibre aspect ratio from measurement of fiber suspention gel point. Cellulose 20: 1885–1896. https://doi.org/10.1007/s10570-013-9972-9
Wahyuningtyas E, Permana M, Wiadnyani S. 2017. Pengaruh jenis pelarut terhadap kandungan senyawa kurkumin dan aktivitas antioksidan ekstrak kunyit (Curcuma domestica Val). J Itepa 6: 61-70.
Wang C, Gao L, Liu M, Xia S, Han Y. 2022. Viscosity reduction mechanism of functionalized silica nanoparticles in heavy oil-water system. Fuel Proc Technol 237: 1-15. https://doi.org/10.1016/j.fuproc.2022.107454
Winuprasith T, Suphantharika M. 2015. Properties and stability of oil-in-water emulsions stabilized by microfibrillated cellulose from mangosteen rind. Food Hydrocolloids 43: 690–699. https://doi.org/10.1016/j.foodhyd.2014.07.027
Zhang J, Jinnai S, Ikeda R, Wada M, Hayashida S, Nakashima K. 2009. Simple HPLC- fluorescence method for quantitation of curcuminoids and its application to turmeric products. Anal Sci 25: 385-388. https://doi.org/10.2116/analsci.25.385