Calcium Bioavailability and Serum Calcium Level in Pregnant Rats After Administration of Milk-Based Drinks Containing Lactic Acid Bacteria
Article Sidebar
Issue
Vol. 16 No. 3 (2021)
Accepted
11 October 2021
Published
29 November 2021
Keywords:
-
bioavailability, calcium, rats, serum, undernourished
Abstract
This study aims to evaluate calcium bioavailability through serum calcium level in pregnant rats treated with two Milk-Based Drinks (MBD) containing Lactic Acid Bacteria (LAB), i.e Lactobacillus Casei Shirota Strain (LcS) drink, and Four Strains Bacteria (FS) (Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus delbrueckii subp. bulgaricus, Streptococcus thermophiles). It was a completely randomized experimental study using 24 Sprague Dawley rats. Rats were divided into one negative control group with a normal nutritional status (A0) and three undernourished groups. The undernourished groups were divided into one positive control group (A1), one group receiving MBD containing LcS group (A2), and another group receiving MBD containing FS (A3). All rats received high protein diets during pregnancy. The intervention was started in early pregnancy (D0) until 19 days of pregnancy (D19). Blood samples were collected at the D0 and D19. No significant differences in food intake were found among the rats in all groups. Administration of MBD containing LAB in A2 and A3 showed significant (p<0.05) increment of calcium bioavailability (30.79±6.88%; 20.44±9.04%). Both MBDs treatment containing LAB showed no significant difference in serum calcium bioavailability (p>0.05). The results suggest that MBDs containing LAB are useful in enhancing calcium bioavailability.
References
Abd El-Gawad IA, Abou Elsamh MM, Saleh FA, Rayan EA. 2014. Bioavailability of Ca, P, and Zn and bone mineralization in rats fed yoghurt and soy-yoghurt containing bifidobacteria. European J Nutr Food Saf 4(2):110−126. https://doi.org/10.9734/EJNFS/2014/6856
Aminah S, Suparmo, Naruki S, Wuryastuty H. 2017. Consumption of elicited soybean sprout flour increases calcium level in serum of ovariectomized rats. Universa Medicina 36(2):94−101. https://doi.org/10.18051/UnivMed.2017.v36.94-101
Benakriche BM, Ali BAM, Zohra IKF, Omar K. 2014. Probiotic oligosaccharides improve the recovery of intestinal mucosa and biochemical parameters in malnourished rats. Int J Nutr Metab 6(2):28−36. https://doi.org/10.5897/IJNAM2013.0159
Black RE, Victoria CG, Walker SP, Bhutta ZA, Christian P, De Onis M, Ezzati M, Grantham-McGregor S, Katz J, Martorell R et al. 2013. Maternal and child malnutrition and overweight in low-income and middle-income countries. The Lancet 382(9890):427−451. https://doi.org/10.1016/S0140-6736(13)60937-X
Camargo EB, Moraes LF, Souza CM, Akutsu R, Barreto JM, da Silva EMK, Betrán AP, Torloni MR. 2013. Survey of calcium supplementation to prevent preeclampsia: The gap between evidence and practice in Brazil. BMC Pregnancy and Childbirth 13(1):1−7. https://doi.org/10.1186/1471-2393-13-206
Dainese-Plichon R, Schneider S, Piche T, Hebuterne X. 2014. Malabsorption et intolérance au lactose chez l’adulte. Nutr Clin et Metab 28(1):46−51. https://doi.org/10.1016/j.nupar.2013.12.002
Estrela DC, Lemes CGC, Guimarães ATB, Malafaia G. 2014. Effects of short-term malnutrition in rats. Scientia Plena 10(07):1−13
Gao Z, Yin J, Zhang J, Ward RE, Martin RJ, Lefevre M, Cefalu WT, Ye J. 2009. Butyrate improves insulin sensitivity and increases energy expenditure in mice. Diabetes 58(7):1509−1517. https://doi.org/10.2337/db08-1637
Gonen E, Sahin I, Ozbek M, Kovalak E, Yologlu E, Ates Y. 2005. Effects of pregnancy and lactation on bone mineral density, and their relation to the serum calcium, phosphorus, calcitonin and parathyroid hormone levels in rats. J Endocrinol Invest 28(6):322−326. https://doi.org/10.1007/BF03347197
Heaney RP. 2002. Protein and calcium: Antagonists or synergists?. Am J Clin Nutr 75(4):609−610. https://doi.org/10.1093/ajcn/75.4.609
Kalkwarf HJ, Specker BL. 2002. Bone mineral changes during pregnancy and lactation. Endocrine 17(1):49−53. https://doi.org/10.1385/ENDO:17:1:49
Karimi G, Sabran MR, Jamaluddin R, Parvaneh K, Mohtarrudin N, Ahmad Z, Khazaai H, Khodavandi A. 2015. The anti-obesity effects of Lactobacillus caseistrain shirota versus Orlistat on high fat diet-induced obese rats. Food Nutr Res 59(1):29273. https://doi.org/10.3402/fnr.v59.29273
[KHNI] Kerry Health and Nutrition Institute. 2021. Ten key health and nutrition trends 2021. https://khni.kerry.com/trends-and-insights/ten-key-health-and-nutrition-trends-of-this-year/ [Accessed 16th August 2020]
[MoH RI] Ministry of Health Republic of Indonesia. 2014. Keluarga Sehat Idamanku Kota Sehat Kotaku. Jakarta (ID): MoH RI.
[MoH RI] Ministry of Health Republic of Indonesia 2018. Situasi Balita Pendek (stunting) di Indonesia. Jakarta (ID): Buletin Jendela Data dan Informasi.
McConnell El, Basit AW, Murdan S. 2008. Measurements of rats and mouse gastroinestinal pH, fluid and lynphoid tissue, and implications for in-vivo experiments. J Pharm Pharmacol 60(1):63−70. https://doi.org/10.1211/jpp.60.1.0008
Lu Z, Ding L, Lu Q, Chen YH. 2013. Tissue barriers claudins in intestines. Tissue Barriers 1(13):e24978. https://doi.org/10.4161/tisb.24978
Millenium Challenge Account Indonesia. 2014. Stunting dan masa depan Indonesia. http://citradenali.info/wp-content/uploads/2018/05/1.2.-Millenium-Challenge-Account-%E2%80%93-Indonesia.-Stunting-dan-Masa-Depan-Indonesia.-www.mca-indonesia.go_.id_..pdf. [Accessed 17th August 2021]
Merino-Sanjuan M, Catalán-Latorre A, Nacher A, Miralles-Arnau S, Jimenez-Torres NV. 2011. Animal model of undernutrition for the evaluation of drug pharmacokinetics. Nutr Hosp 26(6):1296−1300. https://doi.org/10.1590/S0212-16112011000600016
Nurliyani, Kandarina BI, Kusuma S, Trisnasari YD, Prabawati F. 2014. Profile of bacteria and short chain fatty acids of caecaldigesta in malnourished rat fed goat milk yoghurt. J Food Nutr Res 2(12):1015−1020. https://doi.org/10.12691/jfnr-2-12-24
Nyaradi A, Li J, Hickling S, Foster J, Oddy WH. 2013. The role of nutrition in children's neurocognitive development, from pregnancy throung childhood. Front Hum Neurosci 7:97. https://doi.org/10.3389/fnhum.2013.00097
Prentice A. 2000. Calcium in pregnancy and lactation. Annu Rev Nutr 20(1):249−272. https://doi.org/10.1146/annurev.nutr.20.1.249
Pusparini, Ernawati F, Hardinsyah H, Briawan D. 2016. Indeks massa tubuh rendah pada awal kehamilan dan defisiensi vitamin A pada trimester kedua sebagai faktor risiko gangguan pertumbuhan linier pada bayi lahir. J Gizi Pangan 11(3):191−200. https://doi.org/10.25182/jgp.2016.11.3.%p
Purnasari G, Briawan D, Dwiriani CM. 2016. Asupan kalsium dan tingkat kecukupan kalsium pada ibu hamil di kabupaten Jember. Jurnal MKMI 12(4):261−268.
Rahayu BD. 2020. Feed conversion ratio tikus selama masa kebuntingan dan pengaruhnya terhadap bobot dan jumlah anak. [Undergraduate thesis]. Bogor (ID): IPB University.
Raveschot C, Coutte F, Frémont M, Vaeremans M, Dugersuren J, Dembelel S, Driver D, Dhulster P, Flahaut C, Cudennec B. 2020. Probiotic Lactobacillus strains from Mongolia improve calcium transport and uptake by intestinal cells in vitro. Food Res Int 133:109201. https://doi.org/10.1016/j.foodres.2020.109201
Renyoet BS. 2016. Estimasi potensi kerugian ekonomi akibat stunting dan obesitas pada balita di Indonesia. [Thesis]. Bogor (ID): IPB University.
Savaiano DA. 2014. Lactose digestion from yoghurt: Mechanism and relevance. Am J Clin Nutr 99(5):1251S−1255S. https://doi.org/10.3945/ajcn.113.073023
Smith SE, Ramos RA, Refinetti R, Farthing JP, Paterson PG. 2013. Protein-energy malnutrition induces an aberrant acute-phase response and modifies the circadian rhythm of core temperature. Appl Physiol Nutr Metab 38(8):844−853. https://doi.org/10.1139/apnm-2012-0420
Solang M. 2017. Blood cockle (Anadaragranosa) supplementation to increase serum calcium level and femur growth of low-protein diet rats. Nusantara Biosci 9(1):62−67. https://doi.org/10.13057/nusbiosci/n090111
Suhartini S. 2013. Identifikasi kadar kalsium pada serum tikus dengan kelainan disharmoni oklusi. Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva 2(2):92−97
Tanida M, Shen J, Maeda K, Horii Y, Yamano T, Fukushoma Y, Nagayi K. 2008. High-fat diet induced obesity is attenuated by probiotic strain Lactobacillus paracasei ST11 (NCC2461) in rats. Obes Res Clin Pract 2(3):159−169. https://doi.org/10.1016/j.orcp.2008.04.003
Wu B, Imhoff-Kunsch B, Girard AW. 2012. Biological mechanism for nutritional regulation of maternal health and fetal growth development. Pediatr Perinant Epidemol 26:4−26. https://doi.org/10.1111/j.1365-3016.2012.01291.x
Yongki, Hardinsyah H, Gulardi G, Marhamah M. 2014. Status gizi awal kehamilan dan pertambahan berat badan ibu hamil kaitannya dengan BBLR. J Gizi Pangan. 4(1):8−12. https://doi.org/10.25182/jgp.2009.4.1.8-12
Aminah S, Suparmo, Naruki S, Wuryastuty H. 2017. Consumption of elicited soybean sprout flour increases calcium level in serum of ovariectomized rats. Universa Medicina 36(2):94−101. https://doi.org/10.18051/UnivMed.2017.v36.94-101
Benakriche BM, Ali BAM, Zohra IKF, Omar K. 2014. Probiotic oligosaccharides improve the recovery of intestinal mucosa and biochemical parameters in malnourished rats. Int J Nutr Metab 6(2):28−36. https://doi.org/10.5897/IJNAM2013.0159
Black RE, Victoria CG, Walker SP, Bhutta ZA, Christian P, De Onis M, Ezzati M, Grantham-McGregor S, Katz J, Martorell R et al. 2013. Maternal and child malnutrition and overweight in low-income and middle-income countries. The Lancet 382(9890):427−451. https://doi.org/10.1016/S0140-6736(13)60937-X
Camargo EB, Moraes LF, Souza CM, Akutsu R, Barreto JM, da Silva EMK, Betrán AP, Torloni MR. 2013. Survey of calcium supplementation to prevent preeclampsia: The gap between evidence and practice in Brazil. BMC Pregnancy and Childbirth 13(1):1−7. https://doi.org/10.1186/1471-2393-13-206
Dainese-Plichon R, Schneider S, Piche T, Hebuterne X. 2014. Malabsorption et intolérance au lactose chez l’adulte. Nutr Clin et Metab 28(1):46−51. https://doi.org/10.1016/j.nupar.2013.12.002
Estrela DC, Lemes CGC, Guimarães ATB, Malafaia G. 2014. Effects of short-term malnutrition in rats. Scientia Plena 10(07):1−13
Gao Z, Yin J, Zhang J, Ward RE, Martin RJ, Lefevre M, Cefalu WT, Ye J. 2009. Butyrate improves insulin sensitivity and increases energy expenditure in mice. Diabetes 58(7):1509−1517. https://doi.org/10.2337/db08-1637
Gonen E, Sahin I, Ozbek M, Kovalak E, Yologlu E, Ates Y. 2005. Effects of pregnancy and lactation on bone mineral density, and their relation to the serum calcium, phosphorus, calcitonin and parathyroid hormone levels in rats. J Endocrinol Invest 28(6):322−326. https://doi.org/10.1007/BF03347197
Heaney RP. 2002. Protein and calcium: Antagonists or synergists?. Am J Clin Nutr 75(4):609−610. https://doi.org/10.1093/ajcn/75.4.609
Kalkwarf HJ, Specker BL. 2002. Bone mineral changes during pregnancy and lactation. Endocrine 17(1):49−53. https://doi.org/10.1385/ENDO:17:1:49
Karimi G, Sabran MR, Jamaluddin R, Parvaneh K, Mohtarrudin N, Ahmad Z, Khazaai H, Khodavandi A. 2015. The anti-obesity effects of Lactobacillus caseistrain shirota versus Orlistat on high fat diet-induced obese rats. Food Nutr Res 59(1):29273. https://doi.org/10.3402/fnr.v59.29273
[KHNI] Kerry Health and Nutrition Institute. 2021. Ten key health and nutrition trends 2021. https://khni.kerry.com/trends-and-insights/ten-key-health-and-nutrition-trends-of-this-year/ [Accessed 16th August 2020]
[MoH RI] Ministry of Health Republic of Indonesia. 2014. Keluarga Sehat Idamanku Kota Sehat Kotaku. Jakarta (ID): MoH RI.
[MoH RI] Ministry of Health Republic of Indonesia 2018. Situasi Balita Pendek (stunting) di Indonesia. Jakarta (ID): Buletin Jendela Data dan Informasi.
McConnell El, Basit AW, Murdan S. 2008. Measurements of rats and mouse gastroinestinal pH, fluid and lynphoid tissue, and implications for in-vivo experiments. J Pharm Pharmacol 60(1):63−70. https://doi.org/10.1211/jpp.60.1.0008
Lu Z, Ding L, Lu Q, Chen YH. 2013. Tissue barriers claudins in intestines. Tissue Barriers 1(13):e24978. https://doi.org/10.4161/tisb.24978
Millenium Challenge Account Indonesia. 2014. Stunting dan masa depan Indonesia. http://citradenali.info/wp-content/uploads/2018/05/1.2.-Millenium-Challenge-Account-%E2%80%93-Indonesia.-Stunting-dan-Masa-Depan-Indonesia.-www.mca-indonesia.go_.id_..pdf. [Accessed 17th August 2021]
Merino-Sanjuan M, Catalán-Latorre A, Nacher A, Miralles-Arnau S, Jimenez-Torres NV. 2011. Animal model of undernutrition for the evaluation of drug pharmacokinetics. Nutr Hosp 26(6):1296−1300. https://doi.org/10.1590/S0212-16112011000600016
Nurliyani, Kandarina BI, Kusuma S, Trisnasari YD, Prabawati F. 2014. Profile of bacteria and short chain fatty acids of caecaldigesta in malnourished rat fed goat milk yoghurt. J Food Nutr Res 2(12):1015−1020. https://doi.org/10.12691/jfnr-2-12-24
Nyaradi A, Li J, Hickling S, Foster J, Oddy WH. 2013. The role of nutrition in children's neurocognitive development, from pregnancy throung childhood. Front Hum Neurosci 7:97. https://doi.org/10.3389/fnhum.2013.00097
Prentice A. 2000. Calcium in pregnancy and lactation. Annu Rev Nutr 20(1):249−272. https://doi.org/10.1146/annurev.nutr.20.1.249
Pusparini, Ernawati F, Hardinsyah H, Briawan D. 2016. Indeks massa tubuh rendah pada awal kehamilan dan defisiensi vitamin A pada trimester kedua sebagai faktor risiko gangguan pertumbuhan linier pada bayi lahir. J Gizi Pangan 11(3):191−200. https://doi.org/10.25182/jgp.2016.11.3.%p
Purnasari G, Briawan D, Dwiriani CM. 2016. Asupan kalsium dan tingkat kecukupan kalsium pada ibu hamil di kabupaten Jember. Jurnal MKMI 12(4):261−268.
Rahayu BD. 2020. Feed conversion ratio tikus selama masa kebuntingan dan pengaruhnya terhadap bobot dan jumlah anak. [Undergraduate thesis]. Bogor (ID): IPB University.
Raveschot C, Coutte F, Frémont M, Vaeremans M, Dugersuren J, Dembelel S, Driver D, Dhulster P, Flahaut C, Cudennec B. 2020. Probiotic Lactobacillus strains from Mongolia improve calcium transport and uptake by intestinal cells in vitro. Food Res Int 133:109201. https://doi.org/10.1016/j.foodres.2020.109201
Renyoet BS. 2016. Estimasi potensi kerugian ekonomi akibat stunting dan obesitas pada balita di Indonesia. [Thesis]. Bogor (ID): IPB University.
Savaiano DA. 2014. Lactose digestion from yoghurt: Mechanism and relevance. Am J Clin Nutr 99(5):1251S−1255S. https://doi.org/10.3945/ajcn.113.073023
Smith SE, Ramos RA, Refinetti R, Farthing JP, Paterson PG. 2013. Protein-energy malnutrition induces an aberrant acute-phase response and modifies the circadian rhythm of core temperature. Appl Physiol Nutr Metab 38(8):844−853. https://doi.org/10.1139/apnm-2012-0420
Solang M. 2017. Blood cockle (Anadaragranosa) supplementation to increase serum calcium level and femur growth of low-protein diet rats. Nusantara Biosci 9(1):62−67. https://doi.org/10.13057/nusbiosci/n090111
Suhartini S. 2013. Identifikasi kadar kalsium pada serum tikus dengan kelainan disharmoni oklusi. Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva 2(2):92−97
Tanida M, Shen J, Maeda K, Horii Y, Yamano T, Fukushoma Y, Nagayi K. 2008. High-fat diet induced obesity is attenuated by probiotic strain Lactobacillus paracasei ST11 (NCC2461) in rats. Obes Res Clin Pract 2(3):159−169. https://doi.org/10.1016/j.orcp.2008.04.003
Wu B, Imhoff-Kunsch B, Girard AW. 2012. Biological mechanism for nutritional regulation of maternal health and fetal growth development. Pediatr Perinant Epidemol 26:4−26. https://doi.org/10.1111/j.1365-3016.2012.01291.x
Yongki, Hardinsyah H, Gulardi G, Marhamah M. 2014. Status gizi awal kehamilan dan pertambahan berat badan ibu hamil kaitannya dengan BBLR. J Gizi Pangan. 4(1):8−12. https://doi.org/10.25182/jgp.2009.4.1.8-12
Authors
NurwatiY., HardinsyahH., MarliyatiS. A., & FahrudinM. (2021). Calcium Bioavailability and Serum Calcium Level in Pregnant Rats After Administration of Milk-Based Drinks Containing Lactic Acid Bacteria. Jurnal Gizi Dan Pangan, 16(3), 149-158. https://doi.org/10.25182/jgp.2021.16.3.149-158
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The copyright of manuscripts of the article shall be assigned/transferred to Jurnal Gizi dan Pangan (Indonesian Journal of Nutrition and Food)
Article Details
