Asap Cair untuk Pengendalian Bulkholderia glumae dan Pemacu Pertumbuhan Benih Padi
Abstract
Liquid smoke to Control Bulkholderia glumae and Growth Promoter of Rice Seeds
Bacterial grain rot disease caused by Bulkholderia glumae is an important disease that can be transmitted through rice seeds. Various bacterial grain rot disease control techniques have been developed to prevent crop loss, one of which is liquid smoke. Liquid smoke has been widely studied as an agent for controlling various plant diseases. The aim of the study was to determine the potential of liquid smoke to control bacterial grain rot disease caused by B. glumae and to promote the growth of rice seedlings. Research was conducted in some stages, included: potential test of liquid smoke against B. glumae, phytotoxicity test of liquid smoke and its effect on rice seed growth; and test the effectiveness of liquid smoke in suppressing B. glumae on infected seeds. The results showed that liquid smoke with a concentration of 2% could suppress the development of B. glumae in vitro and did not cause phytotoxicity in rice seeds with a vigor index of 90% and germination of 90% compared to controls with a vigor index value and germination value of 76% and 83%. Liquid smoke with a concentration of 2% can also reduce the severity of disease in the nursery phase with a relative inhibition level of 43.8%. The value of the length of the plumule of rice seedlings in the 2% liquid smoke treatment was 5.87 cm. The plumule value was greater than the control which was only 5.22 cm. In contrast to the plumula, the value of radicular length did not increase significantly compared to the control.
Downloads
References
Aisyah I, Giyanto, Sinaga MS, Nawangsih AA, Pari G. 2018. Uji in vitro asap cair terhadap Ralstonia syzygii subsp. celebesensis penyebab penyakit darah pisang. Jurnal Fitopatologi Indonesia. 14(4):145–151. DOI: https://doi.org/10.14692/jfi.14.4.145.
Baharuddin, Harniati R, Faisal F. Yani A. Suparni, Hamid H, Kuswinanti T, Jahuddin R. 2017. Keberadaan penyakit busuk bulir (Burkolderia glumae) pada tanaman padi di Sulawesi Selatan. Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia; 2017 Jan 10; Bogor (ID): IPB University. hlm 19–16.
[BPS] Badan Pusat Statistik. 2021. Produksi padi tahun 2021 turun 0.43 persen (angka tetap). https://www.bps.go.id/pressrelease/2022/03/01/1909/produksi-padi-tahun-2021-turun-0-43-persen--angka-tetap-.html [diakses 20 Juni 2022].
Chien CC, Chang YC. 1987. The susceptibility of rice plants at different growth stages and of 21 commercial rice varieties to Pseudomonas glumae. Journal of Agricultural Research of China. 36:302–310.
Fazlara A, Ekhtelat M. 2012. The disinfectant effects of benzalkoniumchloride on some important foodborne pathogens. American-Eurasian Journal of Agricultural and Environmental Sciences. 12(1):23–29.
Flematti GR, Waters MT, Scaffidi A, Merritt DJ, Ghisalberti EL, Dixon KW, Smith SM. 2013. Karrikin and cyanohydrin smoke signals provide clues to new endogenous plant signaling compounds. Molecular Plant. 6 (1):29–37. DOI: https://doi.org/10.1093/mp/sss132.
Flórez-Zapata N, Uribe-Vélez D. 2011. Determinación de la Infección de Burkholderia glumae en Semillas de Variedades Comerciales Colombianas de Arroz. Revist Facultad Nacional de Agronomia Medellín. 64(2):6093–6104.
Hart H. 1991. Organic Chemistry A Short Course Eight Edition. Bonston (US): Houghton Mifflin Company.
Hasibuan M, Safni I, Lisnawita, Lubis K. 2018. Morphological characterization of several strains of the rice-pathogenic bacterium Burkholderia glumae in North Sumatra. International Conference on Agriculture, Environment, and Food Society. IOP Conference Series: Earth and Environmental Science: 122(2018) 012044:1–4. DOI: https://doi.org/10.1088/1755-1315/122/1/012044.
Ibrahim AY. 2019. Keefektifan kitosan untuk pengendalian nematoda pucuk putih padi (Aphelenchoides besseyi Cristie) melalui perlakuan benih [skripsi]. Bogor (ID): IPB University.
ISTA [International Rules for Seed Testing Asosiation]. 2010. International Rules for Seed Testing Ed 2010. Switzerland (CH): The International Seed Testing Association.
Jeong Y, Kim J, Suhyun K, Yongsung K. 2003. Toxoflavin produced by Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops. Plant Disease. 87(8):890–894. DOI: https://doi.org/10.1094/PDIS.2003.87.8.890.
Joko T. 2017. Burkholderia glumae sebagai emerging pathogen: status, potensi kerusakan, dan strategi pengendalian. Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia; 2017 Jan 10; Bogor (ID): IPB University. hlm 27–35.
Karseno, Darmadji P, Rahayu K. 2002. Daya hambat asap cair karet terhadap bakteri pengkontaminasi lateks dan ribbed smoke sheet. Agritech. 21(1):10–15. DOI: https://doi.org/10.22146/agritech.13639.
Komarayati, Gusmailina, Pari G. 2013. Arang dan cuka kayu: produk hasil hutan bukan kayu untuk meningkatkan pertumbuhan tanaman dan serapan hara karbon. Jurnal Penelitiaian Hasil Hutan. 31(1):49–62. DOI: https://doi.org/10.20886/jphh.2013.31.1.49-62.
Lee YH, Ko SJ, Cha KH, Chi, HG, Lee DG, Noh TH, Lee SD, Han KS. 2004. Micro-weather factors during rice heading periode influencing the development of rice bacterial grain rot. Research in Plant Disease.10(3):167–174. DOI: https://doi.org/10.5423/RPD.2004.10.3.167.
Lu W, Pan L, Zao H, Jia Y, Wang Y, Yu X, Wang X. 2014. Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves. The Crop Journal. 2:398–406. DOI: https://doi.org/10.1016/j.cj.2014.06.005.
Luo J, Xie G, Li B, Lihui X. 2007. First report of Burkholderia glumae isolated from symptomles rice seeds in china. The American Phytopatholgical Society. 91 (10):1363. DOI: https://doi.org/10.1094/PDIS-91-10-1363B.
Nasution SP. 2018. Penyakit busuk bakteri (Bulkholderia glumae) terbawa benih padi berbagai varietas dan konfirmasinya dengan PCR [skripsi]. Bogor (ID): IPB University.
Nugroho A, Aisyah I. 2013. Efektivitas asap cair dari limbah tempurung kelapa sebagai biopestisida benih di gudang penyimpanan. Jurnal Penelitian Hasil Hutan. 31(1):1–8. DOI: https://doi.org/10.20886/jphh.2013.31.1.1-8.
Nurrahmawan ME, Giyanto, Nawangsihn AA, Sulistiani E. 2021. Asap cair sebagai pemacu pertumbuhan dan ketahanan tanaman pisang terhadap Ralstonia syzygii subsp. celebesensis. Jurnal Fitopatologi Indonesia. 17(5):183–194. DOI: https://doi.org/10.14692/jfi.17.5.183-194.
Pedraza-Herrera LA, Bautista JP, Cruz-Ramírez CA, Uribe-Vélez D. 2020. IBUN2755 Bacillus strain controls seedling root and bacterial panicle blight caused by Burkholderia glumae. Biological Control. 153:104494. DOI: https://doi.org/10.1016/j.biocontrol.2020.104494.
Purnama RGS, Mutaqin KH, Tondok ET. 2018. Keefektifan asap cair dan elektroterapi untuk mengeliminasi infeksi Xanthomonas oryzae pv. oryzae pada benih padi. Jurnal Fitopatologi Indonesia. 14(2):54–62. DOI: https://doi.org/10.14692/jfi.14.2.54.
Sabbineni J. 2016. Phenol: an effective antibacterial agent. Journal of Medicinal and Organic Chemistry. 3(2):182–191.
Schaad NW, Jones JB, Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria Ed ke-3. Minnesota (US): American Phytopatological Society.
Silva SI, Santos Pimenta A, De Oliveira Miranda N, Costa Lourenco YB, Costa De Souza E. 2020. Wood vinegar inhibits emergence and initial growth of Leucaena (Leucaena leucocephala /Lam./ de Wit) seedlings. Agriculturae Conspectus Scientificus. 85(2):153–158.
Suryani L. 2017. Burkholderia glumae, bakteri penyebab hawar pada malai padi. https://www.bkpbanjarmasin1.me/unduhan/berita-pdf/2017/oktober/burkholderia_glumae.pdf [diakses 22 Juli 2022].
Tefa A. 2017. Uji viabilitas dan vigor benih padi (Oryza sativa, L) selama penyimpanan yang berbeda. Savana Cendana. 2(3):48–50. DOI: https://doi.org/10.32938/sc.v2i03.210.
Villaécija-Aguilar JA, Hamon-Josse M, Carbonnel S, Kretschmar A, Schmidt C, Dawid C, Bennett T, Gutjahr C. 2019. SMAX1/SMXL2 regulate root and root hair development downstream of KAI2-mediated signalling in Arabidopsis. PLOS Genetics. 15(8): e1008327. DOI: https://doi.org/10.1371/journal.pgen.1008327.
Widarti A, Giyanto, Mutaqin KH. 2020. Insidensi penyakit busuk bulir padi, identifikasi, dan keragaman bakteri Bulkholderia glumae pada beberapa varietas padi di Jawa Barat. Jurnal Fitopatologi Indonesia. 16(1):9–20. DOI: https://doi.org/10.14692/jfi.16.1.9-20.
Wiyono S, Mutaqin KH, Hidayat SH, Supramana, Widodo, 2017. Emerging disease pada tanaman pertanian: strategi dan opsi kebijakan pengendalian. Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia; 2017 Jan 10; Bogor (ID): IPB University. hlm 1–11.
Yang JF, Yang CH, Liang MT, Gao ZJ, Wu YW, Chuang LY. 2016. Chemical composition, antioxidant, and antibacterial activity of wood vinegar from Litchi chinensis. Molecules. 21(9):1150. DOI: https://doi.org/10.3390/molecules21091150.
Copyright (c) 2022 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.