Patogenisitas Botryodiplodia theobromae pada Batang Cendana dan Penghambatannya secara in Vitro oleh Trichoderma spp.
Abstract
Pathogenicity of Botryodiplodia theobromae on Sandalwood Stems and Its in Vitro Inhibition by Trichoderma spp.
Research was carried out to determine the pathogenicity of Botryodiplodia theobromae in infecting sandalwood stems, and to test the ability of 10 isolates of Trichoderma spp. in inhibiting in vitro growth of two isolates of B. theobromae. The pathogenicity test was carried out by inoculating B. theobromae isolates on sandalwood stems. The antagonist test was conducted using the dual culture method with 10 isolates of Trichoderma spp. and two isolates of B. theobromae as combination treatment. The pathogenicity test proved that B. theobromae caused disease symptoms starting on the day 30th after inoculation. Antagonist test showed that isolates of Trichoderma spp. were able to inhibit the growth of two isolates of B. theobromae through antibiosis and competition activities. The best inhibition against B. theobromae Bt01 was achieved by T. viride TVJKS (53.20%), while the best inhibition against B. theobromae Bt02 was achieved by T. hamatum THAK (53.26%).
Downloads
References
Ali M, Samosir IY. 2021. Uji antagonisme jamur endofit tanaman aren (Arenga pinnata Merr.) terhadap Ganoderma boninense Pat. penyebab penyakit busuk pangkal batang kelapa sawit. Jurnal Agrikultura 32(3):304–311. DOI: https://doi.org/10.24198/agrikultura.v32i3.36611.
Ariyanti M, Asbur Y. 2018. Cendana (Santalum album L.) sebagai tanaman penghasil minyak atsiri. Jurnal Kultivasi. 17(1):558–567. DOI: https://doi.org/10.24198/kultivasi.v17i1.15804.
Barnett HL, Hunter BB. 1998. Illustrated Genera of Imperfect Fungi. APS Press.
Batista E, Lopes A, Alves A. 2020. Botryosphaeriaceae species on forest trees in Portugal: diversity, distribution and pathogenicity. European Journal of Plant Pathology. 158:693–720. DOI: https://doi.org/10.1007/s10658-020-02112-8.
Batista E, Lopes A, Alves A. 2021. What we know about Botryosphaeriaceae? An overveiw of a worldwide cured dataset. Forest. 12(3):313. DOI: https://doi.org/10.3390/f12030313.
Biju CN, Jeevalatha A, Peeran MF, Bhai RS, Basima F, Nissar VAM, Srinivasan V, Thomas L. 2021. Association of Lasiodiplodia theobromae with die-back and decline of nutmeg as revealed through phenotypic, pathogenicity and phylogenetic analyses. 3 Biotech. 11:422. DOI: https://doi.org/10.1007/s13205-021-02961-y.
Dwiastuti WE, Aji TG. 2021. Citrus stem rot disease (Lasiodiplodia theobromae (Pat.) Griff. & Maubl) problem and their control strategy in Indonesia. IOP Conference Series: Earth and Environmental Science. 752(1):012030. DOI: https://doi.org/10.1088/1755-1315/752/1/012030.
Guzmán-Guzmán P, Kumar A, de los Santos-Villalobos S, Parra-Cota, FI, Orozco-Mosqueda MdC, Fadiji AE, Hyder S, Babalola OO, Santoyo G. 2023. Trichoderma species: our best fungal allies in the biocontrol of plant diseases—a review. Plants. 12(3):432. DOI: https://doi.org/10.3390/plants12030432.
Karunanayake KOLC, Adikaram NKB. 2020. Stem-end rot in major tropical and sub-tropical fruit species. Ceylon Journal of Science. 49(5):327–336. DOI: https://doi.org/10.4038/cjs.v49i5.7800.
Khuong NQ, Nhien DB, Thu LTM, Trong ND, Hiep PC, Thuan VM, Quang LT, Thuc LV, Xuan DT. 2023. Using Trichoderma asperellum to antagonize Lasiodiplodia theobromae causing stem-end rot disease on Pomelo (Citrus maxima). Journal of Fungi. 9(10):981. DOI: https://doi.org/10.3390/jof9100981.
Kumar V, Koul B, Taak P, Yadav D, Song M. 2023. Journey of Trichoderma from pilot scale to mass production: A review. Agriculture. 13(10):2022. DOI: https://doi.org/10.3390/agriculture13102022.
Li X, Leng J, Yu L, Bai H, Li X, Wisniewski M, Liu J, Sui Y. 2022. Efficacy of the biocontrol agent Trichoderma hamatum against Lasiodiplodia theobromae on macadamia. Frontiers in Microbiology. 13:994422. DOI: https://doi.org/10.3389/fmicb.2022.994422.
Mukhopadhyay R, Kumar D. 2020. Trichoderma: a beneficial antifungal agent and insights into its mecahnism of biocontrol potential. Egyptian Journal of Pest Control. 30(1):1–8. DOI: https://doi.org/10.1186/s41938-020-00333-x.
Ratnasari JD, Isnawati, Ratnasari E. 2014. Uji antagonis jamur agens hayati terhadap jamur Cercospora musae penyebab penyakit Sigatoka secara in vitro. LenteraBio. 3(2):129–135.
Rossman AY, Allen WC, Castlebury LA. 2017. Proposals to conserve Botryodiplodia theobromae (Lasiodiplodia theobromae) against Sphaeria glandicola, Diplodia gossypina, and Physalosporarhodina (Botryosphaeria rhodina); Phyllosticta yuccae against Leptodothiorella notabilis; and Ramularia brunnea against Sphaerella tussilaginis (Mycosphaerella tussilaginis) (Ascomycota: Dothideomycetes). Taxon. 66(3):747–748. DOI: https://doi.org/10.12705/663.17.
Sakan R, Seran W, Mau AE. 2022. Identifikasi hama pada kayu cendana (Santalum album Linn.) di hutan pendidikan dan pelatihan sisimeni sanam Kabupaten Kupang. Jurnal Wana Lestari. 6(1):65–30.
Salamiah, Badruzsaufari, Arsyad M. 2008. Jenis tanaman inang dan masa inkubasi patogen Botryodiplodia theobromae pat. penyebab penyakit kulit diplodia pada jeruk. Jurnal Hama dan Penyakit Tumbuhan Tropika. 8(2):123–131. DOI: https://doi.org/10.23960/j.hptt.28123-131.
Sandra FK, Nurhasanah YS, Mutaqin KH, Wiyono S, Tondok ET. 2021. Keragaman morfologi dan molekuler Lasiodiplodia theobromae dari tanaman jeruk, kakao, karet, manggis, dan pisang. Jurnal Fitopatologi Indonesia. 17(2):58–66. DOI: https://doi.org/10.14692/jfi.17.2.58-66.
Susanna, Sinaga MS, Wiyono S, Triwidodo H. 2018. Pemanfaatan cendawan antagonis in situ sebagai agens biokontrol Lasiodiplodia theobromae penyebab dieback pada pada di Aceh Selatan. Jurnal Pertanian Tropik. 5(3):447–454. DOI: https://doi.org/10.32734/jpt.v5i3.3119.
Tyskiewicz R, Nowak A, Ozimek E, Jaroszuk-´Sciseł J. 2022. Trichoderma: the current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. Internal Journal of Molecular Science. 23(4):2329. DOI: https://doi.org/10.3390/ijms23042329.
Vitoria NS, Cavalcanti M, Bezerra JL 2012. Lasiodiplodia theobromae: a new host and a revision of plant hosts reported in Brazil. Agrotrópica. 24(1):63–66. DOI: https://doi.org/10.21757/0103-3816.2012v24n1p63-66.
Copyright (c) 2023 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
