Animal model of diabetic keratopathy

  • Pitra Ariesta Shinta Dewi Doctoral Programme of Biomedical Sciences, Faculty of Medicine, University of Indonesia, Jakarta
  • Ratna Sitompul Department of Ophthalmology, Faculty of Medicine, University of Indonesia, Jakarta
  • Jeanne Pawitan Department of Histology, Faculty of Medicine, University of Indonesia, Jakarta
  • Aroem Naroeni Virology and Cancer Pathobiology Research Center, University of Indonesia, Jakarta
Keywords: diabetic keratopathy, animal model, streptozotocin


Diabetic keratopathy is one of the most common ocular complications in diabetes mellitus. Protocol for diabetes induction in rat model also has been established in many centers. Nonetheless, method in developing diabetic keratopathy rat model has not been well covered. Streptozotocin (STZ) -induced diabetes is widely being used as animal diabetic model. The purpose of this study is to obtain an animal model of diabetic keratopathy that can be used to study the morphology, metabolism, and function of cornea in cases where human samples can be difficult to obtain. A single dose STZ (50 mg/kg) was injected intraperitoneally to control and intervention group. Plasma glucose level concentrations were tested in day 3 postinjections. Obtained animal model of diabetic keratopathy, with significant difference of blood glucose level between intervention and control group (P<0.00). Sensibility of cornea was decreased by week 14th in intervention group. Epithelial defect were more prominent in diabetic group. Despite the differences between human and animal characteristic of diabetic keratopathy, the use of animal models has contributed to better understanding of this disease and to examine more effective treatment.


Download data is not yet available.


Bikbova G, Oshitari T, Baba T, Yamamoto S. 2016. Neuronal changes in the diabetic cornea: perspectives for neuroprotection. BioMed Research International. 2016:1-8.

Cousen P, Cackett P, Bennett H, Swa K, Dhillon B. 2007. Tear production and corneal sensitivity in diabetes. Journal of Diabetes and its Complications. 21(6):371-373.

Furman BL. 2015.Streptozotocin‐induced diabetic models in mice and rats. Current Protocols in Pharmacology. 70(1):5-47.

King AJ. 2012. The use of animal models in diabetes research. British Journal of Pharmacology. 166(3):877-894.

Ljubimov AV. 2017. Diabetic complications in the cornea. Vision Research. 139:138-152.

Vieira-Potter VJ, Karamichos D, Lee DJ. 2016. Ocular complications of diabetes and therapeutic approaches. BioMed Research International. 2016: 1-14.

Wild S, Roglic G, Green A, Sicree R, King H. 2004. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 27(5):1047-1053.

Yin J, Huang J, Chen C, Gao N, Wang F, Fu-Shin XY. 2011. Corneal complications in streptozocin-induced type I diabetic rats. Investigative Ophthalmology & Visual Science. 52(9):6589-6596.

How to Cite
DewiP. A. S., SitompulR., PawitanJ., & NaroeniA. (2019). Animal model of diabetic keratopathy. ARSHI Veterinary Letters, 3(3), 57-58.

Gambaran kadar gula darah tikus wistar diabetes hasil induksi streptozotocin dosis tunggal

Imron Rosyadi, Ella Romadhona, Ajeng Tyas Utami, Yayik Nur Hijrati, Christin Marganingsih Santosa