Profil protein lambung tikus model ulkus peptikum hasil induksi aspirin dengan terapi ekstrak daun katuk (Sauropus androgynus)

  • Janice Enola Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Brawijaya, Malang
  • Sasangka Prasetyawan Laboratorium Biokimia, Fakultas MIPA, Universitas Brawijaya, Malang
  • Dian Vidiastuti Bagian Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Brawijaya, Malang
DOI: https://doi.org/10.29244/avl.2.1.9-10
Keywords: pita protein, SDS PAGE, ulkus peptikum, daun katuk

Abstract

Ulkus peptikum dapat menyebabkan kerusakan jaringan yang menginduksi terjadinya perubahan profil protein berupa Heat Shock Proteins (HSPs). Penelitian ini memelajari efek antioksidan flavonoid daun katuk untuk pengobatan ulkus peptikum hasil induksi aspirin pada tikus. Tikus putih (Rattus norvegicus) sebanyak 20 ekor dibagi menjadi 5 kelompok perlakuan yaitu kontrol positif (ulkus peptikum), kontrol negatif, terapi P1 (8,1 mg/100 gram BB), terapi P2 (16,2 mg/100 gram BB), dan terapi P3 (24,3 mg/100 gram BB). Perlakuan diberikan selama 14 hari. Induksi ulkus peptikum dilakukan dengan pemberian aspirin 200 mg/kg BB selama 5 hari secara peroral. Isolasi protein lambung dan penentuan profil protein dilakukan dengan metode SDS-PAGE. Hasil penelitian menunjukkan bahwa protein yang muncul adalah HSP70, HSP60, HSP47, dan ubiquitin. HSP47dengan ukuran protein 47 kDa muncul pada kelompok P2 dan P3 sedemikian sehingga dapat diduga sebagai marker dalam proses kesembulan ulcer pada kasus ulkus peptikum.

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Published
2018-02-01
How to Cite
EnolaJ., PrasetyawanS., & VidiastutiD. (2018). Profil protein lambung tikus model ulkus peptikum hasil induksi aspirin dengan terapi ekstrak daun katuk (Sauropus androgynus). ARSHI Veterinary Letters, 2(1), 9-10. https://doi.org/10.29244/avl.2.1.9-10
Issue
Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Section
Articles

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