Pengaruh serai (Cymbopogon citratus) terhadap profil darah dan kadar asam urat pada tikus

Serai (Cymbopogon citratus) adalah tanaman obat dengan sifat antioksidan dan antimikroba. Studi ini mengevaluasi efek infus serai 20% dan 80% pada profil hematologi dan kadar asam urat pada tikus Sprague Dawley. Tiga puluh tikus dibagi menjadi tiga kelompok: kelompok kontrol, kelompok infus 20%, dan kelompok infus 80%. Hasil penelitian menunjukkan tidak ada perbedaan signifikan (P>0,05) pada parameter hematologi, meskipun terjadi fluktuasi pada indeks eritrosit dan leukosit. Infus 80% mengurangi parameter eritrosit, menunjukkan potensi anemia makrositik hipokromik, sedangkan infus 20% mengurangi jumlah leukosit dan kadar asam urat. Temuan ini menunjukkan bahwa infus serai memiliki efek imunomodulator dan anti-hiperurisemia, meskipun konsentrasi yang lebih tinggi dapat memengaruhi eritrosit.

■ INTRODUCTION

Lemongrass (Cymbopogon citratus) is a medicinal plant with antibacterial, anti-inflammatory, antioxidant, and antimicrobial properties. Its essential oil contains citral, which is known for its antimicrobial activity, along with flavonoids, terpenes, tannins, phenolics, and ascorbic acid (Howarto et al., 2015). These compounds reduce inflammation and boost immune responses. Flavonoids act as antispasmodic agents, benefiting patients with neurological disorders (Shah et al., 2011). Lemongrass exhibits antioxidant, antidiabetic, and antimalarial effects, and its citral-based aroma has anxiolytic properties (Ariska & Utomo, 2020).

Studies have explored physiological effects of lemongrass supplementation on hematological parameters, lipid profiles, immune responses, and biochemical indicators in broilers (Al-Awadi & Al-Nadawi, 2020), lambs(Al-Janabi et al., 2023), quails (Alagawany et al., 2021), and rodents (Ekpenyong et al., 2015). These studies examined dietary supplementation and essential oil extract. Evidence on the effects of lemongrass infusion at varying concentrations on blood profiles and uric acid levels remains limited. This study aimed to evaluate effects of 20% and 80% lemongrass infusion on blood profiles and uric acid levels in rats.

■ MATERIALS AND METHODS

This study used a completely randomised design (CRD) with 30 ten-week-old male Sprague Dawley rats randomly divided into three groups: control, 20% lemongrass infusion, and 80% lemongrass infusion. After a two-week acclimatisation, the rats received 1 mL of infusion daily for two weeks, with food and water ad libitum. Blood samples were collected in week 2 from the lateral caudal vein under local anaesthesia. Uric acid levels were measured using an EasyTouch GCU meter. The remaining 0.1 mL of blood was collected in EDTA tubes and analysed using an Alovision HM560V analyser for haematological parameters. Data were analysed using analysis of variance (ANOVA).

■ RESULTS AND DISCUSSION

Figure 1 shows no significant differences in haematological parameters among rats treated with 20% and 80% Cymbopogon citratus infusions compared to controls (P>0.05), despite fluctuations. The 80% lemongrass infusion reduced the RBC count, HGB, and HCT levels, decreased the MCH and MCHC, and increased the MCV. This suggests normocytic or mildly macrocytic hypochromic anaemia, possibly due to bone marrow toxicity or oxidative stress. In hypochromic macrocytic anaemia, reductions in HGB and HCT levels are common (Rahayu et al., 2017).

Figure 1.Hematological profile and uric acid of rats after administered lemongrass infusion. (A) Red blood cell, (B) white blood cells, and (C) uric acid levels. Note: red blood cell (RBC), haemoglobin (Hb), haematocrit (HCT), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), white blood cells (WBC), neutrophil (Net), lymphocyte (Lym), monocyte (Mon), eosinophil (Eos), and basophil (Bas).

Lemongrass infusion affects the leukocyte profile. The 20% infusion reduced leukocyte, lymphocyte, and monocyte counts compared to the control, indicating immunosuppression from citral and flavonoids in Cymbopogon citratus (Shah et al., 2011). Higher neutrophil, eosinophil, and basophil counts in the 80% infusion group indicate immune response activation, as citral promotes immune cell recruitment (Tavares et al., 2015). Lemongrass infusion modulates leukocyte dynamics based on its concentration.

In addition to haematological effects, the lemongrass infusion influenced uric acid levels. The 20% infusion reduced uric acid concentrations more effectively than the 80% infusion, showing anti-hyperuricaemic activity. This effect stems from bioactive compounds such as flavonoids, saponins, and citral, which exhibit diuretic properties and may inhibit xanthine oxidase activity (Adeneye & Agbaje, 2007). This reduction may be related to the antioxidant activity of lemongrass, which can decrease oxidative stress and inflammation associated with hyperuricaemia (Ariska & Utomo, 2020).

■ CONCLUSION

The haematological profiles of rats receiving 20% and 80% lemongrass infusion showed no significant changes. However, erythrocyte parameters declined in the 80% group, suggesting potential hypochromic and macrocytic anaemia. The 20% infusion decreased total leukocyte, lymphocyte, monocyte counts, and uric acid levels.

Referensi

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