A Case Study Dugaan mikoplasmosis hemotropik kucing pada kucing domestik dengan anemia, trombositopenia, dan mimisan

Mikoplasmosis hemotropik kucing adalah penyakit yang ditularkan melalui vektor yang signifikan pada kucing, menyebabkan anemia dan penyakit sistemik. Seekor kucing domestik jantan berusia 3 tahun (3,9 kg) menunjukkan penurunan nafsu makan, gejala pernapasan, dan mimisan. Meskipun telah divaksinasi lengkap, pengendalian ektoparasit dan antelmintik tidak konsisten. Pemeriksaan menunjukkan skor kondisi tubuh 3/5, dehidrasi 7%, selaput lendir pucat, pengisian kapiler yang memanjang, hipertermia (40 °C), dan infestasi kutu. Hematologi menunjukkan leukopenia, limfopenia, granulositopenia, trombositopenia, dan anemia normositik normokromik. Tes FPV negatif. Apusan darah menunjukkan organisme yang sesuai dengan Mycoplasma spp. hemotropik. Kucing tersebut didiagnosis dengan dugaan mikoplasmosis hemotropik kucing. Pengobatan meliputi doksisiklin (10 mg/kg PO setiap 24 jam), asam traneksamat (0,1 mL/kg IM), aminofilin (20 mg/kg PO), Ornipural (5 mL selama 5 hari), dan terapi suportif dengan vitamin B kompleks dan zat besi. Setelah 17 hari dirawat di rumah sakit, nafsu makan, aktivitas, dan parameter hematologi kucing membaik secara signifikan.

■ INTRODUCTION

Vector-borne diseases (VBDs) are arthropod-transmitted infections that remain a major cause of global morbidity and mortality, particularly in tropical and subtropical regions, where vector exposure is highly prevalent (Díaz-Regañón et al., 2018). Among feline VBDs, feline hemotropic mycoplasmosis is of particular clinical importance. This disease is caused by hemotropic Mycoplasma spp., which parasitise the surface of erythrocytes and are recognised as among the most pathogenic hemotropic bacterial infections in cats. In affected animals, untreated infection may progress to severe haemolytic anaemia and can ultimately be fatal (Tasker, 2018). The clinical presentation is often variable and is largely influenced by the host immune response, with commonly reported signs including pale mucous membranes, pyrexia, lethargy, anorexia, tachycardia, cardiac murmurs, weight loss, hepatomegaly, splenomegaly, lymphadenomegaly, and different degrees of haemolytic anaemia (Strandberg et al., 2023). Despite the recognised susceptibility of cats to this infection, clinically documented cases of feline hemotropic mycoplasmosis-particularly those accompanied by haemolytic anaemia -remain relatively limited in the literature. Consequently, additional well-documented clinical reports are needed to broaden the current understanding of disease presentation and to support improvements in diagnostic and therapeutic approaches.

■ CASE

Signalment and anamnesis: A 3-year-old male cat (3.9 kg) was presented with decreased appetite, flu-like signs, and epistaxis. Previous tests revealed thrombocytopenia. Vaccination was complete; however, ectoparasite control and deworming were inconsistent.

Physical examination: Pyrexia (40°C), respiratory rate of 32 breaths/min, heart rate of 120 beats/min, prolonged capillary refill time (>2 s), decreased skin turgor (>2 s), body condition score of 3/5, and pale mucous membranes.

Laboratory examinations: Haematological evaluation revealed leukopenia, lymphopenia, granulocytopenia, thrombocytopenia, and normocytic normochromic anaemia. FPV test was negative. Diff-Quik staining revealed hemotropic mycoplasma-like organisms on erythrocytes.

Differential diagnoses: feline panleukopenia, ehrlichiosis/anaplasmosis, FeLV-or FIV-associated haematologic disorders, immune-mediated haemolytic anaemia, coagulopathy, and other infectious or inflammatory anaemia causes.

Diagnosis: Presumptive feline haemotropic mycoplasmosis.

Prognosis: Favorable (fausta).

Treatment: Doxycycline (10 mg/kg PO q24 h), tranexamic acid (0.1 mL/kg IM), aminophylline (20 mg/kg PO), ornipural (5 mL once daily for 5 days), and supportive care.

Table 1.Hematological findings in a 3-year-old male domestic cat.

Parameters	Results	Normal range (Schalm et al., 2010)
White Blood Cells (×10³/µL)	2.5	5.5–19.5
Lymphocyte (%)	17.0	12–45
Granulocyte (%)	78.5	35–85
Monocyte (%)	4.5	2–9
Lymphocyte (×10³/µL)	0.4	0.8–7.0
Granulocyte (×10³/µL)	2.0	2.1–15.0
Monocyte (×10³/µL)	0.1	0.0–1.9
Red Blood Cell (×10⁶/µL)	4.9	4.6–10.0
Hemoglobin (g/dL)	7.1	9.3–15.3
Hematocrit (%)	23.4	28–49
MCV (fL)	48.0	39–52
MCH (pg)	14.5	13–21
MCHC (g/dL)	30.3	30–38
RDW-CV (%)	26.1	14–18
RDW-SD (fL)	41.1	20–80
Platelet (×10³/µL)	49.0	100–514

Figure 1.Laboratory findings in a 3-year-old male cat with presumptive feline hemotropic mycoplasmosis. (A) FPV rapid test result. (B) Diff-Quik-stained blood smear showing Mycoplasma-like organisms on erythrocytes (1000×).

■ RESULTS AND DISCUSSION

This report describes a 3-year-old cat with presumptive feline hemotropic mycoplasmosis, likely due to Mycoplasma haemofelis, associated with severe anaemia. Haematological abnormalities included leukopenia, lymphopenia, and granulocytopenia, suggesting a parasitic or bacterial infection ( Table 1). Decreased erythrocyte count, haemoglobin, and haematocrit levels indicated anaemia (Amelia et al., 2019). Although microcytic hypochromic anaemia is often linked to iron and copper deficiencies (Weiss & Wardrop, 2011), cytological and clinical findings supported this profile. The feline panleukopenia virus (FPV) rapid test was negative (Figure 1), making FPV-associated cytopenia unlikely. Blood smear evaluation showed bacterial organisms resembling Mycoplasma haemofelis attached to erythrocytes.

Definitive confirmation was not achieved as the diagnosis relied on blood smears and rapid testing, both with limited sensitivity. Thus, polymerase chain reaction (PCR), especially quantitative PCR (qPCR), is the gold standard for confirming feline hemotropic mycoplasma infection (Martínez-Díaz et al., 2013). Treatment focused on doxycycline as antimicrobial therapy, effective at oral doses of 5-10 mg/kg once daily (Purba et al., 2020)(Satriawan & Octaviani, 2021). Symptomatic therapy included tranexamic acid to minimise haemorrhage and aminophylline for bronchoconstriction. Ornipural was used as a hepatoprotective agent. Additional care included vitamin B complex, vitamin C, Sangobion, Neurobion, Hepaq, and Fu Fang (1 mL/day). Preventive management addressed vector-related infection through sanitation and ectoparasite control using Advocate with imidacloprid and moxidectin (Maslakah & Kusumarini, 2023). Clinical and haematological improvements were noted, with values normalising by day 15, and the cat was discharged healthy on day 17.

■ CONCLUSION

Early recognition and appropriate management of presumptive feline hemotropic mycoplasmosis may result in favourable clinical and haematological outcomes.

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