Gambaran histologi ovarium sapi aceh pascavitrifikasi menggunakan dimetyl sulfoksida dengan konsentrasi berbeda
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This study aims to determine the morphology of Aceh bovine ovarian pasca vitrification, after its been exposed to dimetyl sulfoksida (DMSO) cryoprotectant. This study use a completely randomized design of one-way pattern ANOVA acquire three replications. Ovaries used in this study are Aceh cattle ovarian which collected from slaughter house (RPH) accounted as 4 organs, to the next sliced into 9 pieces. Ovarian pieces are then grouped into 3 treatment groups namely ovaries which exposed into solution containing DMSO 10% (P1), 20% (P2), and 30% (P3). The results showed that the average number of normal follicles is highest at P1 41,33 ± 32,51; followed by P2 20,00 ± 16,09 , and P3 15,66 ± 10,50. It was concluded that the ovarian tissue was exposed with DMSO 10% was able to maintain of the normal follicle than DMSO 20% and 30% although statistical test results showed no significant difference between group (P>0,05).
Djuwita I, Boediono A, Rusiyantono Y, Mohamad K, Herliatien. 2000. Perkembangan oosit kambing setelah maturasi, fertilisasi dan kultur in vitro. Media Veteriner. 7(4):11-17.
Donnez J, Martinez-Madrid B, Jadoul P, Van Langendonckt A, Demylle D. 2006. Ovarian tissue cryopreservation and transplantation: a review. Hum. Reprod. Update. 12(5):519-535. DOI: https://doi.org/10.1093/humupd/dml032
Fukui EJ, Xia L, Downey BR. 1993. Ultrastructural changes in bovine oocyte cryopreserved by vitrification. Cryobiology. 32(2):139-156. DOI: https://doi.org/10.1006/cryo.1995.1013
Kasai M. 2002. Advances in the cryopreservation of mammalian oocytes and embryos: development of ultra rapid vitrification. Rev. 1:1-9. DOI: https://doi.org/10.1046/j.1445-5781.2002.00004.x
Lucci CM, Scheier LL, Machado GM, Amorim CA, Bao SN. 2007. Effect of storing pig ovaries at 4 or 20°C for different periods of time on the morphology and viability of pre-antra follicles. Reprod. Dom. Anim. 42:76-82. DOI: https://doi.org/10.1111/j.1439-0531.2006.00734.x
Newton H, Fisher J, Arnold JR, Pegg DE, Faddy MJ, Gosden RG. 1998. Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation. Hum. Reprod. 13:376-380. DOI: https://doi.org/10.1093/humrep/13.2.376
Pamungkas FA. 2010. Pemanfaatn metode vitrifikasi untuk kriopreservasi oosit mamalia. Wartazoa. 20(3):112-116.
Shaw JM, Oranratnachai A, Trounson AO. 2000. Fundamental cryobiology of mammalian oocytes and ovarian tissue. Theriogenology. 53:59-72. DOI: https://doi.org/10.1016/S0093-691X(99)00240-X
Steel RGD, Torrie JH. 1993. Prinsip dan Prosedur Statistik. (Diterjemahkan oleh: Soemantri). Gramedia: Jakarta.
Vieira AD, Mezzalira A, Barbieri DP, Lehmkuhl RC, Rubin MIB, Vajta G. 2002. Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology. 45:91-94. DOI: https://doi.org/10.1016/S0011-2240(02)00109-8
Wahjuningsih S, Hardjopranjoto S, Sumitro SM. 2010. Pengaruh konsentrasi etilen glikol dan lama paparan terhadap tingkat fertilitas in vitro oosit sapi. Jurnal Kedokteran Hewan. 4(2):61-64.
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