AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN α-GLUKOSIDASE OLEH EKSTRAK ETANOL BAKTERI ASAM LAKTAT INDIGENUS
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Published
12 June 2019
Keywords:
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α-glucosidase inhibition, antidiabetes, antioxidant, lactic acid bacteria
Abstract
Lactic acid bacteria (LAB) are one of Indonesia's biodiversity which can be beneficial for food and health purposes. Some of LAB are potential probiotics with specific functional properties, such as antidiabetes. This study evaluated the effect of ethanol extracts of twelve indigenous LAB in inhibiting α-glucosidase enzyme and their antioxidant activities. Assay for the α-glucosidase inhibition was performed on LAB ethanol extract using spectrophotometric method at λ=410 nm, while the antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method at λ=517 nm. The results showed that α-glucosidase inhibition was significantly different between the isolates (P<0.01). Lactobacillus fermentum S21209 had the highest α-glucosidase inhibition activity, which was significantly different from Lactobacillus plantarum MB427, Lactobacillus plantarum Pi28a, Lactobacillus delbrueckii W24802 and Lactobacillus plantarum 2 W22409. Evaluation of the antioxidant activity also showed significant difference between the isolates (P<0.01). Lactobacillus plantarum BSL had the highest antioxidant activity (92.81±1.36%), which was not significantly different from vitamin C as a control. This preliminary study reported that twelve indigenous LAB could be used as potential antidiabetic probiotics, although the responsible compounds are not known.
References
Bajpai VK, Han JH, Nam GJ, Majumder R, Park C, Lim J, Paek WK, Rather IA, Park YH. 2016. Characterization and pharmacological potential of Lactobacillus sakei 1I1 isolated from fresh water fish Zacco koreanus. J Pharm Sci 24: 1-12. DOI: 10.1186/s40199-016-0147-8.
Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chen W. 2014a. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control 35: 65-72. DOI: 10.1016/j.food cont.2013.06.027.
Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chen W. 2014b. Oral admi-nistration of Lactobacillus rhamnosus CCFM05 28 improves glucose tolerance and cytokine secretion in high-fat-fed, streptozotocin-induced type 2 diabetic mice. J Funct Food 10: 318-326. DOI: 10.1016/j.jff.2014.06.014.
Daliri EBM, Lee BH. 2015. New perspectives on probiotics in health and disease. Food Sci Hum Wellness 4: 56-65. DOI: 10.1016/j.fshw.2015. 06.002.
Emmawati A, Jenie BSL, Nuraida L, Syah D. 2015. Karakterisasi isolat bakteri asam laktat dari mandai yang berpotensi sebagai probiotik. Agritech 35: 146-155. DOI: 10.22146/agritech. 9400.
Emmawati A. 2014. Kajian Antiinfeksi Isolat Bakteri Asam Laktat Asal Mandai [Disertasi]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.
FAO/WHO. 2001. Report of a joint FAO WHO expert consultation on evaluation of health and nutritio-nal properties of probiotics in food including powder milk with lactic acid becateria, American Cordoba Park Hotel, Cordoba, Argentina.
Kodali VP, Sen R. 2008. Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. J Biotechnol 3: 245-251. DOI: 10.1002/biot.200700208.
Kusumawati N. 2002. Seleksi Bakteri Asam Laktat Indigenus Sebagai Galur Probiotik dengan Ke-mampuan Mempertahankan Keseimbangan Mikroflora Feses dan Mereduksi Kolesterol Se-rum Darah Tikus [Tesis]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.
Lacroix IM, Li-Chan EC. 2013. Inhibition of dipeptidyl peptidase (DPP)-IV and α-glucosidase activities by pepsin-treated whey proteins. J Agric Food Chem 61: 7500-7506. DOI: 10.1021/jf401000s.
Li X, Wang N, Yin B, Fang D, Zhao J, Zhang H, Wang G, Chen W. 2016. Lactobacillus planta-rum X1 with α-glucosidase inhibitory activity ameliorates type 2 diabetes in mice. RSC Adv 6: 63536-63547. DOI: 10.1039/C6RA10858J.
Maryati Y, Nuraida L, Dewanti-Hariyadi R. 2016. Kajian bakteri asam laktat dalam menurunkan kolesterol secara in vitro dengan keberadaan oligosakarida. Agritech 36: 196-205. DOI: 10.22 146/agritech.12865
Meyer H, Weidmann H, Lalk M. 2013. Methodologi-cal approaches to help unravel the intracellular metabolome of Bacillus subtilis. Microb Cell Fact 12: 69. DOI: 10.1186/1475-2859-12-69.
Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J. 2015. Probiotics as potential anti-oxidants: A systematic review. J Agric Food Chem 63: 3615-3626. DOI: 10.1021/jf506326t.
Muganga L, Liu X, Tian F, Zhao J, Zhang H, Chen W. 2015. Screening for lactic acid bacteria based on antihyperglycaemic and probiotic po-tential and application in synbiotic set yoghurt. J Funct Food 16: 125-136. DOI: 10.1016/j.jff.20 15.04.030.
Nuraida L, Kartika S. Touw KS, Nurdini AL, Suliantari. 2015. Lactic acid bacteria in tempe fermentation and their potency as probiotic. Paper dipresentasikan pada Seminar Nasional PATPI, Semarang 20-21 Oktober 2015.
Nuraida L, Hana, AW Hartanti, E Prangdimurti. 2012. Evaluasi potensi Lactobacillus yang diiso-lasi dari air susu ibu untuk mencegah diare. J Teknol Industri Pangan 13: 158-165. DOI: 10.6066/jtip.2012.23.2.158.
Nyanzi R, Shuping DSS, Jooste PJ, Eloff JN. 2015. Antibacterial and antioxidant activity of extracts from selected probiotic bacteria. J Food Res 4: 122-132. DOI: 10.5539/jfr.v4n5p122.
Panwar H, Calderwood D, Grant IR. Grover S, Green BD. 2014. Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha- and beta-gluco-sidases suggesting anti-diabetesic potential. Eur J Nutr 53: 1465-1474. DOI: 10.1007/s0039 4-013-0649-9
Patel S, Majumder A, Goyal A. 2012. Potentials of exopolysaccharides from lactic acid bacteria. Indian J Microbiol 52: 3-12. DOI: 10.1007/s1208 8-011-0148-8.
Patten DA, Laws AP. 2015. Lactobacillus produced exopolysaccharides and their potential health benefits - a review. Benef Microb 6: 457-471. DOI: 10.3920/BM2014.0117.
Ramchandran L, Shah NP. 2009. Effect of exopoly-saccharides and inulin on the proteolytic, angio-tensin-I-converting enzyme and α-glucosidase inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage.
Dairy Sci Tech 89: 583-600. DOI: 10.1051/dst/2009039.
Sadishkumar V, Jeevaratnam K. 2016. In vitro pro-biotik evaluation of potential antioxidant lactic acid bacteria isolated from idli batter fermented with Piper betle leaves. Int J Food Sci Tech 52: 329-340. DOI: 10.1111/ijfs.13284.
Salazar-Aranda R, Perez-Lopez LA, Lopez-Arroyo J, Alaniz-Garza BA, de Torres NW. 2011. Antimic-robial and antioxidant activities of plants from Northeast of Mexico. J Evid-Bas Complem Altern Med 41: 233-236. DOI: 10.1093/ecam/ nep127.
Sales PM, Souza PM, Simeoni LA, Magalhaes PO, Silveira D. 2012. α-Amylase inhibitors - a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci 15: 141-183. DOI: 10.18433/J35S3K.
Sancheti S, Sancheti S, Seo SY. 2009. Chaenome-les Sinensis: A potent α-and β-glucosidase inhi-bitor. Am J Pharmacol Tox 4: 8-11. DOI: 10.384 4/ajptsp.2009.8.11.
Seo BJ, Bajpai VK, Rather IA, Park YH. 2015. Par-tially purified exopolysaccharide from Lactoba-cillus plantarum YML009 with total phenolic content, antioxidant and free radical scavenging efficacy. Indian J Pharm Educ 49: 282-292. DOI: 10.5530/ijper.49.4.6.
Son SH, Jeon HL, Jeon EB, Lee NK, Park YS, Kang DK, Paik HD. 2017. Potential probiotic Lactoba-cillus plantarum Ln4 from kimchi: evaluation of β-galactosidase and antioxidant activities. LWT-Food Sci Technol 85: 181-186. DOI: 10.1016/j. lwt.2017.07.018.
Suzuki Y, Kosaka M, Shindo K, Kawasumi T, Kimoto-Nira H, Suzuki C. 2013. Identification of antioxidants produced by Lactobacillus planta-rum. Biosci Biotechnol Biochem 77: 1299-1302. DOI: 10.1271/bbb.121006.
Tang W, Xing Z, Li C, Wang J, Wang Y. 2017. Molecular mechanism and in vitro antioxidant effects of Lactobacillus plantarum MA2. Food Chem 221: 1642-1649. DOI: 10.1016/j.food chem.2016.10.124.
Vasiljevic T, Shah NP. 2008. Review: Probiotics-From Metchnikoff to bioactives. Int Dairy J 18: 714-728. DOI: 10.1016/j.idairyj.2008.03.004.
Wang Y, Wu Y, Wang Y, Xu H, Mei X, Yu D, Wang Y, Li W. 2017. Antioxidant properties of probio-tic bacteria. Nutrients 9: 521. DOI: 10.3390/nu9 050521.
Wirawati CU. 2002. Potensi Bakteri Asam Laktat yang Diisolasi dari Tempoyak Sebagai Probio-tik. [Tesis]. Bogor: Sekolah Pascasarjana, Insti-tut Pertanian Bogor.
Zeng Z, Luo J, Zuo F, Zhang Y, Ma H, Chen S. 2016. Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory acti-vity. J Funct Food 20: 486-495. DOI: 10.1016/j.j ff.2015.11.030.
Zhang Z, Zhou Z, Li Y, Zhou L, Ding Q, Xu L. 2016. Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis media-ted by TLR2 in mice. Sci Rep 6: 1-14. DOI: 10.1038/srep36083.
Zhang Y, Zhang H. 2013. Microbiota associated with type 2 diabetes and its related complications. Food Sci Hum Wellness 2:
Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chen W. 2014a. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control 35: 65-72. DOI: 10.1016/j.food cont.2013.06.027.
Chen P, Zhang Q, Dang H, Liu X, Tian F, Zhao J, Chen Y, Zhang H, Chen W. 2014b. Oral admi-nistration of Lactobacillus rhamnosus CCFM05 28 improves glucose tolerance and cytokine secretion in high-fat-fed, streptozotocin-induced type 2 diabetic mice. J Funct Food 10: 318-326. DOI: 10.1016/j.jff.2014.06.014.
Daliri EBM, Lee BH. 2015. New perspectives on probiotics in health and disease. Food Sci Hum Wellness 4: 56-65. DOI: 10.1016/j.fshw.2015. 06.002.
Emmawati A, Jenie BSL, Nuraida L, Syah D. 2015. Karakterisasi isolat bakteri asam laktat dari mandai yang berpotensi sebagai probiotik. Agritech 35: 146-155. DOI: 10.22146/agritech. 9400.
Emmawati A. 2014. Kajian Antiinfeksi Isolat Bakteri Asam Laktat Asal Mandai [Disertasi]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.
FAO/WHO. 2001. Report of a joint FAO WHO expert consultation on evaluation of health and nutritio-nal properties of probiotics in food including powder milk with lactic acid becateria, American Cordoba Park Hotel, Cordoba, Argentina.
Kodali VP, Sen R. 2008. Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. J Biotechnol 3: 245-251. DOI: 10.1002/biot.200700208.
Kusumawati N. 2002. Seleksi Bakteri Asam Laktat Indigenus Sebagai Galur Probiotik dengan Ke-mampuan Mempertahankan Keseimbangan Mikroflora Feses dan Mereduksi Kolesterol Se-rum Darah Tikus [Tesis]. Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.
Lacroix IM, Li-Chan EC. 2013. Inhibition of dipeptidyl peptidase (DPP)-IV and α-glucosidase activities by pepsin-treated whey proteins. J Agric Food Chem 61: 7500-7506. DOI: 10.1021/jf401000s.
Li X, Wang N, Yin B, Fang D, Zhao J, Zhang H, Wang G, Chen W. 2016. Lactobacillus planta-rum X1 with α-glucosidase inhibitory activity ameliorates type 2 diabetes in mice. RSC Adv 6: 63536-63547. DOI: 10.1039/C6RA10858J.
Maryati Y, Nuraida L, Dewanti-Hariyadi R. 2016. Kajian bakteri asam laktat dalam menurunkan kolesterol secara in vitro dengan keberadaan oligosakarida. Agritech 36: 196-205. DOI: 10.22 146/agritech.12865
Meyer H, Weidmann H, Lalk M. 2013. Methodologi-cal approaches to help unravel the intracellular metabolome of Bacillus subtilis. Microb Cell Fact 12: 69. DOI: 10.1186/1475-2859-12-69.
Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J. 2015. Probiotics as potential anti-oxidants: A systematic review. J Agric Food Chem 63: 3615-3626. DOI: 10.1021/jf506326t.
Muganga L, Liu X, Tian F, Zhao J, Zhang H, Chen W. 2015. Screening for lactic acid bacteria based on antihyperglycaemic and probiotic po-tential and application in synbiotic set yoghurt. J Funct Food 16: 125-136. DOI: 10.1016/j.jff.20 15.04.030.
Nuraida L, Kartika S. Touw KS, Nurdini AL, Suliantari. 2015. Lactic acid bacteria in tempe fermentation and their potency as probiotic. Paper dipresentasikan pada Seminar Nasional PATPI, Semarang 20-21 Oktober 2015.
Nuraida L, Hana, AW Hartanti, E Prangdimurti. 2012. Evaluasi potensi Lactobacillus yang diiso-lasi dari air susu ibu untuk mencegah diare. J Teknol Industri Pangan 13: 158-165. DOI: 10.6066/jtip.2012.23.2.158.
Nyanzi R, Shuping DSS, Jooste PJ, Eloff JN. 2015. Antibacterial and antioxidant activity of extracts from selected probiotic bacteria. J Food Res 4: 122-132. DOI: 10.5539/jfr.v4n5p122.
Panwar H, Calderwood D, Grant IR. Grover S, Green BD. 2014. Lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha- and beta-gluco-sidases suggesting anti-diabetesic potential. Eur J Nutr 53: 1465-1474. DOI: 10.1007/s0039 4-013-0649-9
Patel S, Majumder A, Goyal A. 2012. Potentials of exopolysaccharides from lactic acid bacteria. Indian J Microbiol 52: 3-12. DOI: 10.1007/s1208 8-011-0148-8.
Patten DA, Laws AP. 2015. Lactobacillus produced exopolysaccharides and their potential health benefits - a review. Benef Microb 6: 457-471. DOI: 10.3920/BM2014.0117.
Ramchandran L, Shah NP. 2009. Effect of exopoly-saccharides and inulin on the proteolytic, angio-tensin-I-converting enzyme and α-glucosidase inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage.
Dairy Sci Tech 89: 583-600. DOI: 10.1051/dst/2009039.
Sadishkumar V, Jeevaratnam K. 2016. In vitro pro-biotik evaluation of potential antioxidant lactic acid bacteria isolated from idli batter fermented with Piper betle leaves. Int J Food Sci Tech 52: 329-340. DOI: 10.1111/ijfs.13284.
Salazar-Aranda R, Perez-Lopez LA, Lopez-Arroyo J, Alaniz-Garza BA, de Torres NW. 2011. Antimic-robial and antioxidant activities of plants from Northeast of Mexico. J Evid-Bas Complem Altern Med 41: 233-236. DOI: 10.1093/ecam/ nep127.
Sales PM, Souza PM, Simeoni LA, Magalhaes PO, Silveira D. 2012. α-Amylase inhibitors - a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci 15: 141-183. DOI: 10.18433/J35S3K.
Sancheti S, Sancheti S, Seo SY. 2009. Chaenome-les Sinensis: A potent α-and β-glucosidase inhi-bitor. Am J Pharmacol Tox 4: 8-11. DOI: 10.384 4/ajptsp.2009.8.11.
Seo BJ, Bajpai VK, Rather IA, Park YH. 2015. Par-tially purified exopolysaccharide from Lactoba-cillus plantarum YML009 with total phenolic content, antioxidant and free radical scavenging efficacy. Indian J Pharm Educ 49: 282-292. DOI: 10.5530/ijper.49.4.6.
Son SH, Jeon HL, Jeon EB, Lee NK, Park YS, Kang DK, Paik HD. 2017. Potential probiotic Lactoba-cillus plantarum Ln4 from kimchi: evaluation of β-galactosidase and antioxidant activities. LWT-Food Sci Technol 85: 181-186. DOI: 10.1016/j. lwt.2017.07.018.
Suzuki Y, Kosaka M, Shindo K, Kawasumi T, Kimoto-Nira H, Suzuki C. 2013. Identification of antioxidants produced by Lactobacillus planta-rum. Biosci Biotechnol Biochem 77: 1299-1302. DOI: 10.1271/bbb.121006.
Tang W, Xing Z, Li C, Wang J, Wang Y. 2017. Molecular mechanism and in vitro antioxidant effects of Lactobacillus plantarum MA2. Food Chem 221: 1642-1649. DOI: 10.1016/j.food chem.2016.10.124.
Vasiljevic T, Shah NP. 2008. Review: Probiotics-From Metchnikoff to bioactives. Int Dairy J 18: 714-728. DOI: 10.1016/j.idairyj.2008.03.004.
Wang Y, Wu Y, Wang Y, Xu H, Mei X, Yu D, Wang Y, Li W. 2017. Antioxidant properties of probio-tic bacteria. Nutrients 9: 521. DOI: 10.3390/nu9 050521.
Wirawati CU. 2002. Potensi Bakteri Asam Laktat yang Diisolasi dari Tempoyak Sebagai Probio-tik. [Tesis]. Bogor: Sekolah Pascasarjana, Insti-tut Pertanian Bogor.
Zeng Z, Luo J, Zuo F, Zhang Y, Ma H, Chen S. 2016. Screening for potential novel probiotic Lactobacillus strains based on high dipeptidyl peptidase IV and α-glucosidase inhibitory acti-vity. J Funct Food 20: 486-495. DOI: 10.1016/j.j ff.2015.11.030.
Zhang Z, Zhou Z, Li Y, Zhou L, Ding Q, Xu L. 2016. Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis media-ted by TLR2 in mice. Sci Rep 6: 1-14. DOI: 10.1038/srep36083.
Zhang Y, Zhang H. 2013. Microbiota associated with type 2 diabetes and its related complications. Food Sci Hum Wellness 2:
Authors
FaridaE., JenieB. S. L., NuraidaL., & GiriwonoP. E. (2019). AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN α-GLUKOSIDASE OLEH EKSTRAK ETANOL BAKTERI ASAM LAKTAT INDIGENUS. Jurnal Teknologi Dan Industri Pangan, 30(1), 56-63. https://doi.org/10.6066/jtip.2019.30.1.56
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