Isolasi Asam Lemak dan Kadar Pigmen Rumput Laut Cokelat Sargassum crassifolium sebagai Sumber Antioksidan Alami Isolation of Fatty Acid and Pigment Cntent of Sargassum crassifolium as A Source of Natural Antioxidant
Article Sidebar
Accepted
20 December 2022
Published
20 December 2022
Keywords:
-
antioxidant, fatty acid, isolation, pigment
Abstract
Sargassum is a marine product, source of bioaktive compound with many divers biological activity, especially as antioxidant and anticancer. The bioactive compound of Sargassum have been utilized as health product in many Industry such as, food, medicine and cosmetic. Fatty acids, (E)-11-octyloxacyclododec-10-en-2-one were isolated from ethyl acetic fraction of brown seaweed Sargassum crassifolium. The aim of the research is to isolate and purification the fatty acid compound and measure the pigmen content and antioxidant activity of S. crassifolium. The method of reasearc as followed; Sargassum extracted using methanol after that the extract partitioned with hexane and ethyl acetic and rinsed with methanol using Colomn Chromatography (CC). Ethyl acetic was purificated and isolated using CC and Thin Layer Chromatography (TLC) was using to monitored and observed the compounds. Isolate of pure compounds of etil acetic fraction of S. crassifolium (FEtSar) was elucidated by basic NMR spectroscopy, FTIR and ESMS and compared with previous literature. The extract, fractions and isolated compound were tested for their pigment content (carotenoid and fucoxanthin) and antioxidant activity using DPPH. The result of study shown, extract, fraction and pure compound of ethyl acetate fraction of S. crassifolium, (FEtSar), (E)-11-octyloxacyclododec-10-en-2-one revealed have antioxidant compound. Therefore S. crassifolium is potencial as a source of natural antioksidant and in future could be explored as health product.
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Dolorosa MT. Nurjanah. Purwaningsih S. Anwar E. Hidayat T. 2017. Kandungan senyawa bioaktif bubur rumput laut Sargassum plagyophyllum dan Eucheuma cottonii sebagai bahan baku krim pencerah kulit. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(3): 633-644.
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Joung EJ. Gwon WG. Shin T. Jung BM. Choi J. Kim HR. 2017. Antiinflammatory action of the ethanolic extract from Sargassum serratifolium on lipopolysaccharide-stimulated mouse peritoneal macrophages and identification of active components. J Applied Phycology 29: 563–573
[KKP] Kementerian Kelautan dan Perikanan. 2017. KKP sasar rumput laut sebagai komoditas unggulan budidaya. http:// www.kkp.go.id.
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Authors
SangerG., DotulongV., & DamongilalaL. J. (2022). Isolasi Asam Lemak dan Kadar Pigmen Rumput Laut Cokelat Sargassum crassifolium sebagai Sumber Antioksidan Alami: Isolation of Fatty Acid and Pigment Cntent of Sargassum crassifolium as A Source of Natural Antioxidant . Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 475-493. https://doi.org/10.17844/jphpi.v25i3.43033
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