Aktivitas Anticendawan dan Antibakteri Minyak Atsiri Ekstrak Kulit Buah Jeruk Purut terhadap Patogen Penting Tanaman Jeruk
Abstract
Antifungal and Antibacterial Activity of Essential Oils of Kaffir Citrus Fruit Peel Extract Against Important Pathogens of Citrus Plants
Main diseases of citrus plants caused by fungi and bacteria can inhibit plant growth, resulting in a decrease in plant production. Disease controls implemented so far mostly relied on synthetic chemical pesticides which have the potential to have negative impacts on the environment and health. Essential oil from the peel of kaffir lime (Citrus hystric) contains compounds that have the potential to inhibit the growth of pathogens in citrus plants. The aim of this research was to determine the antifungal and antibacterial activity of kaffir lime peel essential oil at five concentrations i.e., 0.5%, 1%, 2%, 3%, and 5% against citrus plant pathogens, namely Colletotrichum gloeosporioides, Fusarium oxysporum, and Xanthomonas axonopodis. The results showed that all essential oil concentrations tested had a low category of inhibitory activity against F. oxysporum, and a medium inhibitory activity against C. gloeosporioides. As on X. axonopodis the essential oil showed strong inhibitory activity at concentrations 3% and 5%. Kaffir lime peel essential oil has the potential as antifungal and antibacterial in citrus plants and can be used as an alternative control technology that is environmentally friendly and safe.
Downloads
References
Ahmed Y, D’Onghia AM, Ippolito A, El Shimy H, Cirvilleri G, Yaseen T. 2012. Phytophthora nicotianae is the predominant Phytophthora species in Citrus nurseries in Egypt. Phytopathologia Mediterranea. 51(3):519–527.
Al-Reza SM, Rahman A, Ahmed Y, Kang SC. 2010. Inhibition of plant pathogens in vitro and in vivo with essential oil and organic extracts of Cestrum nocturnum L. Pestic Biochem Phys. 96(2):86–92. DOI: https://doi.org/10.1016/j.pestbp.2009.09.005.
Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods -a review. International Journal of Food Microbiology. 94(3):223–253. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022.
Chowdappa A, Kousalya S, Kamalakannan A, Gopalakrishnan C, Venkatesan K. 2018. Efficacy of plant oils against Xanthomonas axonopodis pv. punicae. Advances in Research. 17(1):1–5. DOI: https://doi.org/10.9734/air/2018/45031.
Cosentino S, Barra A, Pisano B, Cabizza M, Pirisi FM, Palmas F. 2003. Composition and antimicrobial properties of sardinian Juniperus essential oils against foodborne pathogens and spoilage microorganisms. Journal of Food Protection. 66(7):1288–1291. DOI: https://doi.org/10.4315/0362-028X-66.7.1288.
Dambolena JS, López AG, Cánepa MC, Theumer MG, Zygadlo JA, Rubinstein HR. 2008. Inhibitory effect of cyclic terpenes (limonene, menthol, menthone and thymol) on Fusarium verticillioides MRC 826 growth and fumonisin B1 biosynthesis. Toxicon. 51(1):37–44. DOI: https://doi.org/10.1016/j.toxicon.2007.07.005.
Davis WW, Stout TR. 1971. Disc Plate Method of Microbiological Antibiotic Assay. Journal Applied Microbiology. 22(4):659–665.
Ghorbany M, Jafarpour BRM. 2010. Application of some plant products to control of Fusarium oxysporum f. sp cumini causing cumin wilt. Journal of Plant Protection. 24(1):1–16.
Hong-Thao PT, Mai-Linh NV, Hong-Lien NT, Van HN. 2016. Biological characteristics and antimicrobial activity of endophytic Streptomyces sp. TQR12-4 isolated from elite Citrus nobilis cultivar Ham Yen of Vietnam. International Journal of Microbiology. 13:1–8. DOI: https://doi.org/10.1155/2016/7207818.
Isman MB. 2000. Plant essential oils for pest and disease management. Crop Protection. 19(8-10):603–608. DOI: https://doi.org/10.1016/S0261-2194(00)00079-X.
Lozada MIO, Da Silva PP, Pereira RB, Nascimento WM. 2019. Essential oils in the control of Colletotrichum gloeosporioides f. sp. cepae in onion seeds. Revista Ciencia Agronomica. 50(3):510–518. DOI: https://doi.org/10.5935/1806-6690.20190060.
Mandalari G, Bisignano C, Cirmi S, Navarra M. 2017. Effectiveness of Citrus fruits on Helicobacter pylori. Evidence-Based Complementary and Alternative Medicine. 2017(8379262):1–8. DOI: https://doi.org/10.1155/2017/8379262.
Mekonnen M, Wariyo A, Hilu G. 2019. Antifungal activities of some essential oils against Fusarium oxysporum of rosemary and sage plants. Advances in Crop Science and Technology. 7(1):1000419. DOI: https://doi.org/10.4172/2329-8863.1000419.
Noverita, Jayuska A, Alimuddin A. 2014. Uji aktivitas antirayap minyak atsiri kulit jeruk purut (Cytrus hystric D.C) terhadap rayap tanah (Coptotermes sp). Jurnal Kimia Khatulistiwa. 3(2):19–22.
Noveriza R, Miftakhurohmah M. 2020. Efektivitas ekstrak metanol daun salam (Eugenia Polyantha) dan daun jeruk purut (Cytrus histrix) sebagai antijamur pada pertumbuhan Fusarium oxysporum. Jurnal Penelitian Tanaman Industri. 16(1):6–11.
Pathirana HN, Wimalasena, De Silva BCJ, Hossain S, Heo GJ. 2018. Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus). Archives of Polish Fisheries. 26(2):131–139. DOI: https://doi.org/10.2478/aopf-2018-0014.
Perczak A, Gwiazdowska D, Marchwińska K, Juś K, Gwiazdowski R, Waśkiewicz A. 2019. Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds. Archives of Microbiology. 201(8):1085–1097. DOI: https://doi.org/10.1007/s00203-019-01673-5.
Pina-Vaz C, Rodrigues AG, Pinto E, Costa DOS, Tavares C, Salgueiro L, Martinez OJ. 2004. Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology. 18(1):73–78. DOI: https://doi.org/10.1111/j.1468-3083.2004.00886.x.
Setiawan D. 2000. Atlas Tumbuhan Obat di Indonesia. Jakarta (ID): Trubus Agriwidya.
Sreepian A, Sreepian PM, Chanthong C, Mingkhwancheep T, Prathit P. 2019. Antibacterial activity of essential oil extracted from Citrus hystrix (Kaffir Lime) peels: An in vitro study. Tropical Biomedicine. 36(2):531–541.
Srisukh V, Bunyapraphatsara N, Pongpan A, Tungrugsasut W, Puttipipatkhachorn S, Oniam W, Thongbainoi W. 2012. Fresh produce antibacterial rinse from Kaffir Lime oil. Mahidol University Journal of Pharmaceutical Sciences. 39(2):15–27.
Warsito W, Noorhamdani N, Sukardi S, Dwi SR. 2017. Microencapsulation of Cytrus hystrix oil and its activity test as an antimicrobial agent. Journal of Enviromental Engineering and Sustainable Technology. 4(2):131–137. DOI: https://doi.org/10.21776/ub.jeest.2017.004.02.9.
Copyright (c) 2023 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
