Antagonistic Activity of Endophytic Fungi from Dayak Onions’ Flower (Eleutherine bulbosa (Mill.) Urb.) Against Fusarium sp. Infecting Chili Plant
Abstract
Antagonistic Activity of Endophytic Fungi from Dayak Onions’ Flower (Eleutherine bulbosa (Mill.) Urb.) Against Fusarium sp. Infecting Chili Plant
Wilt disease caused by Fusarium spp. is an important problem in chili cultivation. Meanwhile, use of biological agents such as endophytic fungi from medicinal plants have the potential to control plant diseases. Dayak onions are medicinal plants that known to have antibacterial, anti-fungal, anti-inflammatory, and antioxidant properties. Therefore, a study was conducted to determine the antagonistic activity of endophytic fungi isolated from Dayak onion flowers against Fusarium spp. A total of seventeen isolates of endophytic fungi were obtained from the flower of Dayak onion flowers, namely EnA, EnB, EnC, EnD, EnE, EnF, EnG, EnH, EnI, EnJ, EnK, EnL, EnM, EnN, EnO, EnP, and EnQ. Five of the seventeen isolates, i.e. EnA, EnF, EnI, EnJ, and EnK were further examined in antagonistic test against Fusarium spp. using dual culture method. Inhibition of colony growth of Fusarium spp. caused by isolates EnA, EnF, EnI, EnJ, and EnK were 67.6%, 53.15%, 77.25%, 70.42% and 67.1%, respectively.
Downloads
References
Djafaruddin. 2000. Dasar-dasar Pengendalian Penyakit Tanaman. Jakarta (ID): PT Bumi Aksara.
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. J Food Drug Anal. 22(3):296–302. DOI: https://doi.org/10.1016/j.jfda.2013.11.001.
Elbert W, Taylor PE, Andreae MO, Pöschl U. 2007. Contribution of fungi to primary biogenic aerosols in the atmosphere: Wet and dry discharged spores, carbohydrates and inorganic ions. Atmos Chem Phys. 7(17):4569–4588. DOI: https://doi.org/10.5194/acp-7-4569-2007.
Gao FK, Dai CC, Liu XZ. 2010. Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res. 4(13):1346–1351.
Harahap I, Elsie, Nurjanah I. 2017. Isolasi dan seleksi cendawan endofit dari tanaman betadin (Jatropha multifida L.) dan potensinya sebagai antimikroba. J Photon. 7(2):109–114. DOI: https://doi.org/10.37859/jp.v7i02.514.
Kloeppe JW, Rodriguez-Kabana R, Zehnder AW, Murphy JF, SikoraE, Fernandez C. 1999. Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Aus Plant Pathol. 28:21–26. DOI: https://doi.org/10.1071/AP99003.
Izzatinnisa’, Utami U, Mujahidin A. 2020. Uji antagonisme beberapa fungi endofit pada tanaman kentang terhadap Fusarium oxysporum secara in vitro. JRBA. (2)1:18–25. DOI: https://doi.org/10.26740/jrba.v2n1.p18-25.
Nahdah F, Sari N, Rizali A, Wahdah R. 2020. Antagonisme fungi endofit daun jarak pagar (Jatropha curcas) terhadap Fusarium oxysporum C2 penyebab busuk umbi pada bawang merah in Vitro. Agrotechnol Res J. 4(1):47-53. DOI: https://doi.org/10.20961/agrotechresj.v4i1.41351.
Nurzannah SE, Lisnawita, Darma B. 2014. Potensi cendawan endofit asal cabai sebagai agens hayati untuk mengendalikan layu fusarium (Fusarium oxysporum) pada cabai dan interaksinya. JAET. (2)3:1230– 1238.
Reckow V, Widayat W, Rijai L. 2016. Jamur endofit dari umbi bawang dayak (Eleutherine palmifolia Merr.). Di dalam: Prosiding Seminar Nasional Kefarmasian Ke-4; 2016 Jan 11; Samarinda (ID): Universitas Mulawarman. hlm 377–383. DOI: https://doi.org/10.25026/mpc.v4i1.208.
Sarwono E, Nurdin M, Prasetyo J. 2013. Pengaruh kitosan dan Trichoderma sp. terhadap keparahan penyakit antraknosa (Colletotrichum capsici (Syd.) Butl. Et Bisby) pada buah cabai (Capsicum annuum L.). J Agrotek Tropika. 1(3):336–340. DOI: http://dx.doi.org/10.23960/jat.v1i3.2061.
Shi P, Du W, Wang Y, Teng X, Chen X, Ye L. 2019. Total phenolic, flavonoid content, and antioxidant activityof bulbs, leaves, and flowers made from Eleutherine bulbosa (Mill.) Urb. Food Sci Nutr. 7(1):148–154. DOI: https://doi.org/10.1002/fsn3.834.
Strobel GA. 2003. Endophytes as sources of bioactive products. Microbes Infect. 5(6):535–544. DOI: https://doi.org/10.1016/S1286-4579(03)00073-X.
Sutejo AM, A. Priyatmojo, A. Wibowo. 2008. Identifikasi morfologi beberapa spesies jamur fusarium. JPTI. 14(1):7–13.
Vinale F, Ghisalberti EL, Sivasithamparam K, Marra R, Ritieni A, Ferracane R, Woo S, Lorito M. 2009. Factors affecting the production of Trichoderma harzianum secondary metabolites during the interaction with different plant pathogens. Lett Appl Microbiol. 48(6):705–711. DOI: https://doi.org/10.1111/j.1472-765X.2009.02599.x.
Wongpia A, Lomthaisong K. 2010. Changes in the 2DE protein profiles of chilli pepper (Capsicum annum) leavesin response to Fusarium oxysporum infection. Sci Asia. 36(4):259–270. DOI: https://doi.org/10.2306/scienceasia1513-1874.2010.36.259.
Yulianto E. 2014. Evaluasi potensi beberapa jamur agens antagonis dalam menghambat patogen Fusarium sp. pada tanaman jagung (Zea mays L.) [tesis]. Bengkulu (ID): Universitas Bengkulu.
Zuhria SA, Djauhari S, Muhibuddin A. 2017. Exploration and antagonistic test of endophytic fungi from soybean (Glycine max L. Merr) with different resistance to Sclerotium rolfsii. The Journal of Experimental Life Science. 6(2):101–105. DOI: https://doi.org/10.21776/ub.jels.2016.006.02.08.
Copyright (c) 2022 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
