Aktivitas Antagonis Bakteri Endofit Asal Mangrove terhadap Ralstonia solanacearum dan Meloidogyne spp.
Abstract
The main diseases of tomato caused by wilt bacteria (Ralstonia solanacearum) and root knot nematodes (Meloidogyne spp.) may cause significant yield losses and need to be managed. Recently, biocontrol approach especially using endophytic bacteria has been developed to control plant pathogens. This research was aimed to obtain endophytic bacteria from 2 species of mangrove, which effectively controlled root knot nematode and wilt bacteria. A total of 843 endophytic bacteria were successfully isolated from 2 species of mangroves. The bacteria isolates were further subjected for biosafety assay. The results of the test showed that 403 endophytic bacteria gave negative reaction in hypersensitive and hemolytic tests. Furthermore, 19 isolates effectively suppressed the growth R solanacearum and killed Meloidogyne in in vitro test. Physiological test showed that 14 and 11 isolates of the bacteria were able to produce protease and chitinase, respectively. This research provides a new information that endophytic bacteria from mangrove has a potency as a biocontrol agents.
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