Potensi Dark Septate Endophyte Isolat Sumatera Utara terhadap Ganoderma boninense Penyebab Busuk Pangkal Batang Kelapa Sawit
Potential of Dark Septate Endophytes North Sumatra Isolates Against Ganoderma boninense the Pathogen of Basal Stem Rot of Oil Palm
Abstract
Potential of Dark Septate Endophytes North Sumatra Isolates Against Ganoderma boninense the Pathogen of Basal Stem Rot of Oil Palm
Basal stem rot caused by Ganoderma boninense causes significant damage to oil palm plantations in Southeast Asia, including in Indonesia. Intensive use of chemical fungicides against Ganoderma can negatively affect the environment, health, and disrupt the ecological balance. Application of microbe as biocontrol agents using antagonistic endophytic fungi, among others is dark septate endophyte (DSE), is potential as management control strategy for Ganoderma. The aim of this experiment is to obtain local DSE fungi isolates from North Sumatra from Ganoderma endemic areas with the potential for biological control agents to G. boninense. DSE fungi exploration was carried out in the area with moderate incidence of Ganoderma (5%-30%) in Galang District, Deli Serdang Regency and the test was conducted in vitro. Five isolates of DSE fungi was obtained, namely II 3 A CM, Gelap A, II 4 CM, II 3 A CC, and abu-abu. In vitro inhibition of G. boninense by DSE isolates at 7 days after inoculation are Gelap A isolate (100%), abu-abu isolate (90.87%), II 3 A CC (82.83%), II 3 A CM (82.23%), and II 4 CM (74.88%), respectively. This finding provides basic data about potential of DSE fungi as a biological agent for G. boninense and it is the first report for DSE fungi origin from oil palm in North Sumatra.
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References
Alexander A, Dayou J, Chong, KP. 2015. Morphological changes of Ganoderma boninense mycelia after challenged by Trichoderma and Bacillus. 1669:020075. DOI: https://doi.org/10.1063/1.4919213.
Chong KP, Dayou J, Alexander A. 2017. Detection and Control of Ganoderma boninense in Oil Palm. Cham (CH): Springer. hlm 1–12. DOI: https://doi.org/10.1007/978-3-319-54969-9_1.
Dalimunthe CI, Soekarno BPW, Munif A, Surono. 2019. Seleksi dan uji potensi dark septate endophyte sebagai agens hayati penyakit jamur akar putih (Rigidoporus microporus) pada tanaman karet. Jurnal Penelitian Tanaman Karet. 37(1):11–20. DOI: https://doi.org/10.22302/ppk.jpk.v37i1.624.
Dewi IP, Maryono T, Aeny TN, Ratih S. 2015. Kemampuan Trichoderma sp. dan filtratnya dalam menekan pertumbuhan Sclerotium rolfsii secara in vitro. Jurnal Agrotek Tropika, 3(1):130–133. DOI: https://doi.org/10.23960/jat.v3i1.1974.
Goncalves TAP, Nisgoski S, Oliveira JS, Marcati CR, Ballarin AW, Muniz GIB. 2018. A contribution to the identification of charcoal origin in Brazil III: microscopic identification of 10 Cerrado species. Australian Journal of Botany. 66(3):255–264. DOI: https://doi.org/10.1071/BT17196.
Handayani D. 2016. Keberadaan cendawan cendawan dark septate endophyte (DSE) pada sistem perakaran benih Shorea selanica. Eksakta. 1:38–44.
Handayani D. 2017. Karakteristik cendawan dark septate endophyte (DSE) pada akar tanaman jagung dan padi. Eksakta. 18(1):61–68. DOI: https://dx.doi.org/10.24036/eksakta/vol18-iss01/20.
Izzatinnisa, Utami U, Mujahidin A. 2020. Uji antagonisme beberapa fungi endofit pada tanaman kentang terhadap Fusarium oxysporum secara in vitro. Jurnal Riset Biologi dan Aplikasinya. 2(1):18–25. DOI: https://doi.org/10.26740/jrba.v2n1.p18-25.
Jumpponen A, Trappe JM. 1998. Dark septate endophytes: a review of facultative biotrophic root colonizing fungi. New Phytologist. 140(2):295–310. DOI: https://doi.org/10.1046/j.1469-8137.1998.00265.x.
Kartika T. 2013. Viabilitas, parameter, dan tolok ukur viabilitas benih Di dalam: Widajati E, Murniati E, Palupi ER, Kartika T, Suhartanto MR, Qadir A. Dasar Ilmu dan Teknologi Benih. Edisi 1. Bogor (ID): IPB Press.
Liswarni Y, Nurbailis, Busniah M. 2018. Eksplorasi cendawan endofit dan potensi-nya untuk pengendalian Phytophthora palmivora penyebab penyakit busuk buah kakao. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. 4(2):231–235.
Mushimiyimana I, Kimonyono A, Nsabimana P. 2016. Colonial and morphological characteristics of various fungi species isolated from soil in Bangalore city. Bulletin of Environment, Pharmacology and Life Sciences. 6(1):17–21.
Nair DN, Padmavathy S. 2014. Impact of endophytic microorganisms on plants, environment and humans. The Scientific World Journal. 2014:250693. DOI: https://doi.org/10.1155/2014/250693.
Paterson RRM. 2019. Ganoderma boninense disease of oil palm to significantly reduce production after 2050 in Sumatra if projected climate change occurs. Microorgnanisms. 7(1):24. DOI: https://doi.org/10.3390/microorganisms7010024.
Rahayu BR, Proborini MW, Darmayasa IBG. 2019. Isolasi, identifikasi dan persentase keberadaan hifa jamur endofit pada tanaman gemitir (Tagetes erecta L.) di beberapa daerah di Bali. Metamorfosa: Journal of Biological Sciences. 6(1):75–82. DOI: http://dx.doi.org/10.24843/metamorfosa.2019.v06.i01.p12.
Ridgway R. 1912. Color standards and color nomenclature. Washington DC: Published by the Author. DOI: https://doi.org/10.5962/bhl.title.144788.
Sajar S. 2018. Karakteristik kultur Corynespora cassiicola (Berk. & Curt) Wei dari berbagai tanaman inang yang ditumbuhkan di media PDA. Agrium: Jurnal Ilmu Pertanian. 21(3):210–217.
Sukmawati D, Miarsyah M. 2017. Pathogenic activity of Fusarium equiseti from plantation of citrus plants (Citrus nobilis) in the village Tegal Wangi, Jember Umbulsari, East Java, Indonesia. Asian Journal of Agriculture and Biology. 5(4):202–213.
Suomela JA, Vajanto K, Raisanen R. 2018. Seeking nettle textiles–utilizing a combination of microscopic methods for fibre identification. Studies in Conservation. 63(7):412–422. DOI: https://doi.org/10.1080/00393630.2017.1410956.
Surono, Narisawa K. 2017. The dark septate endophytic fungus Phialocephala fortinii is a potential decomposer of soil organic compounds and a promoter of Asparagus officinalis growth. Fungal Ecology. 28:1–10. DOI: http://dx.doi.org/10.1016/j.funeco. 2017.04.001.
Susanto A, Prasetyo AE, Hari P, Wening S. 2013. Ganoderma boninense penyebab penyakit busuk batang atas kelapa sawit. Jurnal Fitopatologi Indonesia. 9(4):123–126. DOI: https://doi.org/10.14692/jfi.9.4.123.
Triasih U, Abadi AL, Muhibbudin A, Widyaningsih S. 2022. Uji beberapa jamur antagonis terhadap Colletotrichum gloeosporiodes penyebab penyakit busuk buah apel manalagi (Malus sylvestris) secara in vitro. Di dalam: Agropross: National Conference Proceedings of Agriculture; 2022 Okt 19; Jember (ID): Politeknik Negeri Jember. hlm 389–397. DOI: https://doi.org/10.25047/agropross.2022.309.
Verma SK, Kharwar RN, Gond SK, Kingsley KL, White JF. 2019. Exploring endophytic communities of plants: methods for assessing diversity, effects on host development and potential biotechnological applications. Di dalam: Seed endophytes. Cham (CH): Springer Nature Switzerland. hlm 55–82. DOI: https://doi.org/10.1007/978-3-030-10504-4_4.
Watanabe T. 2018. Pictorial Atlas of Soilborne Fungal Plant Pathogens and Diseases. Boca Raton (FL): CRC Press.
Wicaksono WA, Buana RF, Situmorang EC. 2011. Endophyte microbes from oil palm (Elaeis guineensis) tissues and its potential as a biocontrol for Ganoderma boninense in vitro. Di dalam: Proceedings of The 7th ACSA Conference; 2011 Sep 27–30; Bogor (ID): IPB International Convention Center. hlm 360–365.
Yurnaliza, Aryantha INP, Esyanti RR, Susanto A. 2014. Antagonistic activity assessment of fungal endophytes from oil palm tissues against Ganoderma boninense Pat. Plant Pathology Journal. 13(4):257–267. DOI: https://doi.org/10.3923/ppj.2014.257.267.
Zivković S, Stojanović S, Ivanović Ž, Gavrilović V, Popović T, Balaž J. 2010. Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides. Archives of Biological Sciences. 62(3):611–623. DOI: https://doi.org/10.2298/ABS1003611Z.
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