Resistance of Escherichia coli from the cloaca of buffy fish owls (Ketupa ketupu) to several antibiotics

  • Revita Ismahyuningsih Student of Undergraduate Program, School of Veterinary Medicine and Biomedical Sciences, IPB University
  • Usamah Afiff Division of Medical Microbiology, School of Veterinary Medicine and Biomedical Sciences, IPB University
  • Srihadi Agungpriyono Division of Anatomy, School of Veterinary Medicine and Biomedical Sciences, IPB University
Keywords: buffy fish owl (Ketupa ketupu), Escherichia coli, antibiotic resistance


Antibiotic resistance has become a problem worldwide. Although E. coli is a normal flora, there are several pathogenic strains. This study aimed to determine the resistance patterns of E. coli isolates from cloacal swabs of buffy fish owls (Ketupa ketupu) from captive birds to streptomycin, tetracycline, gentamicin, nalidixic acid, ampicillin, and doxycycline using the Kirby-Bauer disc diffusion test. The inhibition zone formed was compared to the Clinical Laboratory Standards Institute (CLSI) 2020. The results showed that all isolates were resistant to ampicillin but were still sensitive to gentamicin. Two isolates remained sensitive to tetracycline, nalidixic acid, doxycycline, and streptomycin. One isolate was intermediate to streptomycin and was resistant to tetracycline, nalidixic acid, and doxycycline.


Download data is not yet available.


[CLSI] Clinical and Laboratory Standards Institute. 2020. Performance standards for antimicrobial susceptibility testing. Edisi ke-30. West Valley (US): Clinical and Laboratory Standards Institute.

Damayanti S, Kusdarwati R, Suprapto H. 2019. Bacterial resistance of Escherichia coli against antibiotics in Clarias batrachus digestion. AACL Bioflux. 12(6): 2195-2201.

Machado DN, Lopes ES, Albuquerque AH, Horn RV, Bezerra WGA, Siqueira RAS, Lopes IT, Nunes FP, Teixeira RSC, Cardoso WM. 2018. Isolation and antimicrobial resistance profiles of enterobacteria from nestling grey-breasted parakeets (Pyrrhura griseipectus). Brazilian Journal of Poultry Science. 20(1): 103-110.

Manishimwe R, MoncadaPM, Bugarel M, Scott HM, Loneragan GH. 2021. Antibiotic resistance among Escherichia coli and Salmonellaisolated from dairy cattlefeces in Texas. PLos One. 16(5): e0242390. | PMid:33961628 PMCid:PMC8104409

Milanda T, Saragih BC, Kusuma SAF. 2014. Deteksi gen resisten-si ampisilin (bla) pada Escherichia coli isolat klinik dengan metode Polymerase Chain Reaction. Indonesian Journal of Clinical Pharmacy. 3(3): 98-106.

Sanches LA, Gomes MDS, Teixeira RHF, Cunha MPV, Oliveira MGXD, Vieira MAM, Gomes TAT, Knobl T. 2017. Captive wild birds as reservoirs of enteropathogenic E. coli (EPEC) and Shiga-toxin producing E. coli (STEC). Brazilian Journal of Microbiology. 48(4): 760-763. | PMid:28619663 PMCid:PMC5628295

Shecho M, Thomas N, Kemal J, Muktar Y. 2017. Cloacael carriage and multidrug resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia. Journal of Veterinary Medicine. 2017: 1-9. | PMid:28349121 PMCid:PMC5350295

Shobrak MY, Abo-Amer AE. 2014. Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escherichia vulneris. Brazilian Journal of Microbiology. 45(4): 1199-1209. | PMid:25763023 PMCid:PMC4323292

How to Cite
IsmahyuningsihR., AfiffU., & AgungpriyonoS. (2023). Resistance of Escherichia coli from the cloaca of buffy fish owls (Ketupa ketupu) to several antibiotics. ARSHI Veterinary Letters, 7(3), 59-60.

Most read articles by the same author(s)