Amylase Inhibition and Free Radical Scavenging Activitites of White Saffron Extract and Fractions

  • Dwiyati Pujimulyani Faculty of Agroindustry, University of Mercu Buana, Yogyakarta
  • Wisnu Adi Yulianto Faculty of Agroindustry, University of Mercu Buana, Yogyakarta
  • Astuti Setyawati Faculty of Agroindustry, University of Mercu Buana, Yogyakarta
  • Seila Arumwardana Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung
  • Annisa Amalia Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung
  • Hanna Sari W. Kusuma Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung
  • Ervi Afifah Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung
Keywords: Amilase, antidiabetes, antioksidan, kunir putih


Diabetes is the most common endocrinal disorder characterized by hyperglycemia and long term complications. It has been reported that oxidative stress and excess of free radicals play major roles in diabetes. Development of antidiabetic drugs has been recently emphasized on natural products with less side effect. Antidiabetic activities of white saffron (Curcuma mangga) have been reported. In this study, antiadiabetic activity of four fractions of C. mangga extract (water, hexane, ethyl acetate, buthanol),  white saffron extract and butylated hydroxytoluene/antioxidant standart) was measured by α-amylase activity assay, while antioxidant activity of those fractions was measured using 2-diphenyl-1-picrylhydrazyl scavanger, and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. These fractions were also compared to antidiabetic drug, acarbose, as control. Ethyl acetate fraction and white saffron extract  showed higher α-amylase inhibitory activity (IC50 = 840,23 dan 363,67  µg/mL) among fractions. Ethyl acetate fraction of C. mangga showed the higher antioxidant activities among fractions as indicated by DPPH activity DPPH (IC50 = 83,95 μg/ mL), and ABTS (IC50 = 27,37 μg/ mL). 


Adnyana I, Abuzaid A, Iskandar E, Kurniati N. 2016. Pancreatic lipase and α-amylase inhibitory potential of mangosteen (Garcinia mangos-tana Linn.) pericarp extract. Int J Med Res Health Sci 5: 23–8.

Alam F, Gan SH, Islam MA. 2016. Updates on managing type 2 diabetes mellitus with natural products: towards antidiabetic drug develop-ment. Curr Med Chem 23: 1-37. DOI: 10.2174/0 929867323666160813222436.

Angel GR, Vimala B, Nambisan B. 2013. Antioxidant and antiinflammatory activities of proteins isolated from eight Curcuma species. Phyto pharmacol 4: 96-105.

Betteridge DJ. 2000. What is oxidative stress? Meta-bolism 49: 3-8.

Brahmachari G. 2011. Bio-Flavonoids with Promising Antidiabetic Potentials: A Critical Survey. In: Tiwari VK, Mishra BB, Edi-tors. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. 187–212. Trivandrum: Research Signpost. Kierala, India.

Chinese Diabetes Society. 2014. Chinese guideline for type 2 diabetes prevention (2013). Chin J Diabetes 22: 2–42.

Derosa G, Maffioli P. 2012. α-Glucosidase inhibitors and their use in clinical practice. Arch Med Sci 8: 899-906. DOI: 10.5114/aoms.2012.31621.

Etoundi C, Kuat D, Ngondi J, Oben J. 2010. Anti-amylase antilipase and antioxidant effects of aqueous extracts of some cameroonian spices. J Nat Products 3: 165–171.

Gondokesumo ME, Kusuma HSW, Widowati W. 2017. α-/β-glucosidase and α-amylase inhi-bitory activities of roselle (Hibiscus sabdariffa L.) ethanol extract. Mol Cell Biomed Sci 1: 123. DOI: 10.21705/mcbs.v1i1.3.

Gulati V, Harding I.H, Palombo EA. 2012. Enzyme inhibitory and antioxidant activities of tradi-tional medicinal plants: potential application in the management of hyperglycemia. BMC Complem Altern M 12: 1472–6882. DOI: 10.1186/1472-6882-12-77.

Hasimun P, Adnyana IK, Valentina R, Lisnasari E. 2016. Potential alpha-glucosidase inhibitor from selected Zingiberaceae family. Asian J Pharm Clin Res 9: 164-167.

Jaitak V, Sharma K, Kalia K, Kumar N, Singh HP, Kaul VK, Singh B. 2010. Antioxidant activity of Potentilla fulgens: An alpine plant of western Himalaya. J Food Compos Anal 23: 142-147. DOI: 10.1016/j.jfca.2009.02.013.

Joshi SR. E, Standl E, Tong N, Shah P, Kalra S, Rathod R. 2015. Therapeutic potential of a -glucosidase inhibitors in type 2 diabetes melli-tus: an evidence-based review. Expert Opin Pharm 16: 1959–1981. DOI: 10.1517/146565 66.2015.1070827.

Kamtekar S, Keer V, Patil V. 2014. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. J Appl Pharmacol Sci 4: 61-65.

Madihah M, Alfina F, Gani YY. 2016. Blood glucose level and pancreas histological section of mice (Mus musculus l.) induced by alloxan after treatment of Curcuma mangga val. Rhi-zome extract. J Biol 20: 64-68. DOI: 10.24843/JBIO UNUD.2016.v20.i02.p04.

Matsui, T., Ueda, T., Oki, T., Sugita, K., Terahara, N., Matsumoto, K., 2001. α-Glucosidase inhibi-tory action of natural acylated anthocyanins 1. Survey of natural pigments with potent inhi-bitory activity. J Agri Food Chem 49: 1948–1951.

Nishiyama T, Mae T, Kishida H, Tsukagawa M, Mimaki Y, Kuroda M, Sashida Y, Takahashi K, Kawada T, Nakagawa K, Kitahara M. 2005. Curcuminoids and sesquiterpenoids in turme-ric (Curcuma longa L.) suppress an increase in blood glucose level in type 2 diabetic KK-Ay

Mice. J Agr Food Chem 53: 959-963. DOI: 10. 1021/jf0483873.

Patel DK, Kumar R, Prasad SK, Sairam K, Hemalatha S. 2011. Antidiabetic and in vitro antioxidant potential of Hybanthus ennea spermus (Linn) F. Muell in streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 1: 316–22. DOI: 10.1016/S2221-1691(11)600 51-8.

Patel JM. 2008. A review of potential health benefits of flavonoids. Lethbridge Undergra-duate Res J 3: 32-35.

Prashanth D, Padmaja R, Samiulla DS. 2001. Effect of certain plant extracts on alpha-amylase activity. Fitoterapia 72: 179-181. DOI: 10.101 6/S0367-326X(00)00281-1.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. 2010. Aktivitas antioksidan dan kadar senyawa fenolik pada kunir putih (Curcuma mangga val.) Segar dan setelah blanching. Agritech 30: 68-74.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. 2010. The effect of blanching treatment on the radical scavenging activity of white saffront (Curcuma mangga Val.), Int Food Res J 17: 615-621.

Pujimulyani D, Raharjo S, Marsono Y, Santoso U. 2012. The effect of blanching on antioxidant activity and glycosides of white saffront (Curcuma mangga Val.), Int Food Res J 19: 617-621.

Rhabasa L, Chiasson JL. 2004. Alpha-Glucosidase Inhibitors, 3rd Ed. John Wiley & Sons Ltd., UK. 1: 901-904.

Rohman A, Riyanto S, Utari D. 2006. Aktivitas antioksidan, kandungan fenolik total dan kan-dungan flavonoid total ekstrak etil asetat buah mengkudu serta fraksi-fraksinya. Majalah Far-masi Indonesia 17: 136-142.

Rotenstein LS, Kozak BM, Shivers JP, Yarchoan M, Close J. 2012. The ideal diabetes therapy: what will it look like? How close are we?. Clin Diabetes 30: 44-53. DOI: 10.2337/diaclin.30.2. 44.

Sales PM, Souza PM, Simeoni LA, Silveira D. 2012. α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Sci 15: 141-183. DOI: 10.18433/J35 S3K.

Shasha D. 2014. Reversed phase HPLC-UV quantitation of BHA, BHT and TBHQ in food items sold in bindura supermarkets, Zimbab-we. Int Res J Pure Appl Chem 4: 578–84. DOI: 10.9734/IRJPAC/2014/10419.

Soeng S, Evacuasiany E, Widowati W, Fauziah N, Manik VT, Maesaroh M. 2015. Inhibitory potential of rambutan seeds extract and frac-tions on adipogenesis in 3T3-L1 cell line. J Exp Integr Med 5: 55-60. DOI: 10.5455/jeim.200 115.or.120.

Tundis R, Loizzo MR, Menichini F. 2010. Natural products as alpha-amylase and alpha-gluco-sidase inhibitors and their hypoglycaemic potential in the treatment of diabetes: an update. Mini-Rev Med Chem 10: 315-31. DOI: 10.2174/138955710791331007.

Wenzig EM, Widowitz U, Kunert O, Chrubasik SF, Bucar E Knauder, Bauer R. 2008. Phytoche-mical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) Pre-parations. Phytomedicine 15: 826-835. DOI: 10. 1016/j.phymed.2008.06.012.

Widowati W, Tati H, Hana R, Tjandrawati M. 2011. Antioxidant activities and plateletaggregation inhibitor of black tea (Camellia sinensis L.) extract andfractions. Medicinal Plants 3: 21-26.

Widowati W, Fauziah N, Herdiman H, Afni M, Afifah E, Kusuma HSW. 2016. Antioxidant and anti aging assays of Oryza sativa extracts, vanillin and coumaric acid. J Nat Remed 16: 88-99. DOI: 10.18311/jnr/2016/7220.

Widowati W, Widyanto RM, Husin W, Ratnawati H, Laksmitawati DR, Setiawan B, Nugrahenny D, Bachtiar I. 2014. Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reac-tive oxygen species activity. J Basic Med Sci 17: 702–9.

Wulan DR, Utomo EP, Mahdi C. 2015. Antidiabetic activity of Ruellia tuberosa L., role of α-amylase inhibitor: in silico, in vitro, and in vivo appro-aches. Biochem Res Int 2015: 249-261.

How to Cite
Pujimulyani, D., Yulianto, W. A., Setyawati, A., Arumwardana, S., Amalia, A., W. Kusuma, H. S., & Afifah, E. (2018). Amylase Inhibition and Free Radical Scavenging Activitites of White Saffron Extract and Fractions. Jurnal Teknologi Dan Industri Pangan, 29(1), 10-18.