The effect of red pomegranate provision on malondialdehyde and blood lactic acid level in rats with maximum physical activity

  • Ahmad Zainuddin Study Program of Nutrition, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia http://orcid.org/0000-0003-0898-7404
  • Hardhono Susanto Anatomic Pathology Section, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
  • Ali Rosidi Nutritional Science Courses, Universitas Muhammadiyah Semarang, Semarang 50273, Indonesia
Keywords: lactic acid, malondialdehyde, maximum physical activity, pomegranate

Abstract

The primary objective of this research was to examine the effect of provision of red pomegranate juice on the levels of malondialdehyde (MDA) and blood lactic acid in Wistar rats with maximum physical activity. The study employed a true experiment design. The pretest-posttest with control group design was done on 35 rats given maximum physical activity and treatment for 28 days. The rats were divided into 5 groups: the control group, group with treatment of multivitamin, group with treatment of red pomegranate 2 ml, 4 ml, and 6 ml. The MDA level was measured by TBARS method and lactic acid levels were analysed using ELISA. Comparison statistical analysis was done using paired t-test, while comparison of mean differences between treatment groups were tested using One-way ANOVA, and followed by LSD as the post hoc test. Provision of red pomegranate juice in a variety of doses significantly decreased the levels of MDA and blood lactic acid (p<0.05). The highest MDA level decrease occurred in the group treated with multivitamin (68.02%) and the highest decrease in the levels of blood lactic acid occurred in the group with 6 ml red pomegranate juice (25.94%). Based on the findings, it can be concluded that red pomegranate juice can lower the levels of MDA and blood lactic acid significantly in rats with maximum physical activity.

Author Biography

Ahmad Zainuddin, Study Program of Nutrition, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia

saya Ahmad Zainuddin merupakan mahasiswa Magister Ilmu Gizi Undip. Topik yang saya ambil untuk bahan Tesis saya mengenai olahraga dan gizi. Fenomena yang sering terjadi yaitu pemikiran bahwa aktivitas yang dilakukan secara berlebihan akan meningkatkan kemampuan, padahal itu salah. Dampak yang terjadi akbiat aktivitas / latihan yang berlebihan akan menyebabkan tingginya radikal bebas dan asam laktat. radikal bebas dan asam laktat yang terbentuk menyebabkan performa menurun. untuk mengatasi hal tersebut dibutuhkan antioksidan yang berperan sebagai pencegah terbentuknya radikal bebas. antioksidan tinggi dan alami terdapat pada buah salah satunya yaitu buah delima.

saya merupakan lulusan sarjana di Universitas negeri semarang jurusan Ilmu Keolahragaan. terima kasih

Published
2019-07-30
Section
Articles