Biomassa Nutrien Hijauan Sistem Ransum In Situ (SRI) dan Evaluasi Taraf Penambahan Indigofera zollingeriana terhadap Fermentabilitas dan Kecernaan In Vitro dalam SRI
Nutrient Biomass of Forage in an In Situ Ration System (IRS) and the Evaluation of Indigofera zollingeriana Inclusion Levels on In Vitro Fermentability and Digestibility in IRS
DOI:
https://doi.org/10.29244/jintp.24.2.70-77Keywords:
Indigofera zollingeriana, in vitro, IRSAbstract
This study aimed to evaluate different inclusion levels of Indigofera zollingeriana on the in vitro fermentability and digestibility of IRS to determine the optimal IRS formulation for breeding sheep. The study was designed as a Randomized Block Design (RBD) with five treatments and five replications as blocks, based on the time of rumen fluid collection. Five dietary treatments (M0-M4) were formulated with I. zollingeriana inclusion levels of 0%, 10%, 20%, 30%, and 40% of the total dietary dry matter. Data were analyzed using Analysis of Variance (ANOVA), followed by Duncan's Multiple Range Test (DMRT) when significant differences occurred (p<0.05). The results showed that increasing I. zollingeriana inclusion levels did not affect ruminal pH, total VFA, partial VFA concentrations, or methane production. However, it significantly decreased (p<0.05) the molar proportion of C2, increased the molar proportion of C3, and decreased the C2/C3 ratio by 10% I. zollingeriana inclusion compared with the other treatments. Meanwhile, increasing the inclusion level of I. zollingeriana significantly (p<0.01) increased IVDMD and IVOMD. It can be concluded that Indigofera zollingeriana improved nutrient quality, rumen fermentability, and in vitro digestibility, with the best results obtained at 40% inclusion.
Downloads
References
Abdullah L, Kumalasari NR, Ramadani J, Diapari D, Sitompul MS & Saputra IE. 2025. Effect of forage nutrient on sheep gas emission in different multi-species cropping system. Journal of. Water and Land. Development. 66 (VII- IX) :53–60.doi:10.24425/jwld.2025.155301.
Abdullah L & Suharlina. 2010. Herbage yield and quality of two vegetative parts of indigofera at different times of first regrowth defoliation. Media Peternakan. 33(1):44–49.
Adnew W, Tsegay BA, Tassew A &Asmare B. 2019. Effect of altitudes and harvesting stages on agronomic responses and chemical composition of Brachiaria grass cultivars in Northwestern Ethiopia. Scientific Papers Animal Science and Biotechnologies 52(2):52.
Antari R, Anggraeny YN, Putri AS, Sukmasari PK, Mariyono NHK, Aprilliza MN & Ginting S. 2022. Nutritive and antinutritive contents of Indigofera zollingeriana: its potency for cattle feed in Indonesia. Livestock Research for Rural Development. 34(2).
AOAC International. 2005. Official Methods of Analysis. 18th Editi. Gaithersburg, Maryland ( US): AOAC International.
Cancino SJ. 2018. Forage accumulation of buffelgrass and Urochloa hybrids at different regrowth ages. Ecosistemas y Recursos Agropecuarios. 5(15):573–581.doi:10.19136/era.a5n15.1634.
Chen H, Wang C, Huasai S & Chen A. 2021. Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows. Scientific Report 11(1):1–11.doi:10.1038/s41598-021-96580-5.
Direktorat Jenderal Peternakan dan Kesehatan Hewan. 2025. Statistik Peternakan dan Kesehatan Hewan 2025. Volume ke-4. Jakarta (ID): Kementerian Pertanian RI.
Dung D Van, Shang W & Yao W. 2014. Effect of crude protein levels in concentrate and concentrate levels in diet on in vitro fermentation. Asian Australasia Journal of Animal Science. 27(6):797–805.
Durand-Chávez LM, Arévalo-Aranda YG, Vásquez-Tarrillo RW, Torres-Jara GP, Díaz-Chuquizuta P & Padilla-Macedo BT. 2026. Productive potential of three Urochloa hybrids in low-fertility soils of the Peruvian Amazon. Open Agricultue Journal . 11(1):1–12.doi:10.1515/opag-2025-0486.
Ernawati A, Abdullah L & Permana IG. 2021. Kandungan dan Serapan Mineral Pucuk Indigofera zollingeriana dari Tanaman dengan Kerapatan Tanam Berbeda. J. Ilmu Nutrisi dan Teknologi. Pakan. 19(2):49–58.doi:doi.org/10.29244/jintp.19.2.49-58.
Ernawati A, Abdullah L, Permana IG & Karti PDMH. 2023. Morphological responses, biomass production and nutrient of Pennisetum purpureum cv. Pakchong under different planting patterns and harvesting ages. Biodiversitas. 24(6):3439–3447.doi:10.13057/biodiv/d240640.
Freitas A, Martins L, Salmazo P, Vendruscolo M. 2019. Production of Brachiaria hybrid convert hd 364 submitted to different doses of nitrogen. Agraracademy. 6(11):292–302.doi:10.18677/agrarian_academy_2019a28.
Ge T, Yang C, Li B, Huang X, Zhao L, Zhang X, Tian L & Zhang E. 2023. High-energy diet modify rumen microbial composition and microbial energy metabolism pattern in fattening sheep. BMC Veterinar Research. 19(1):1–12.doi:10.1186/s12917-023-03592-6.
Hall MB, Larson CC & Wilcox CJ. 2010. Carbohydrate source and protein degradability alter lactation, ruminal, and blood measures. Journal of Dairy Science. 93(1):311–322.doi:10.3168/jds.2009-2552.
Holik S, Malyugina S, Staffa A, Filipek J, Horky P, Kadek R & Illek J. 2025. Effect of dietary plant tannin supplementation on rumen fermentation and enteric methane production. Journal of Animal Feed Science. 34(2):272–283.doi:10.22358/jafs/195648/2025.
Hunegnaw B, Mekuriaw Y, Asmare B & Mekuriaw S. 2022. Morphoagronomical and nutritive performance of Brachiaria grasses affected by soil type and fertilizer application grown under rainfed condition in Ethiopia. Advances in Agriculture. 2022:1–11.doi:10.1155/2022/7373145.
Janssen PH. 2010. Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics. Animal Feed Science and Technology. 160(1–2):1–22.doi:10.1016/j.anifeedsci.2010.07.002.
Jayanegara A, Leiber F & Kreuzer M. 2012. Meta-analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. Journal. Animal
Physiology and Animal Nutrition. (Berl). 96(3):365–375.doi:10.1111/j.1439-0396.2011.01172.x.
Khorrami B, Khiaosa-ard R & Zebeli Q. 2021. Models to predict the risk of subacute ruminal acidosis in dairy cows based on dietary and cow factors: A meta-analysis. Journal of Dairy Science. 104(7):7761–7780.doi:10.3168/jds.2020-19890.
Khotijah L, Wiryawan KG, Agus Setiadi M & Astuti DA. 2015. Reproductive performance, cholesterol and progesterone status of garut ewes fed ration containing different levels of sun flower oil. Pakistan Journal of Nutrition. 14(7):388–391.doi:10.3923/pjn.2015.388.391.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2026 M Syarif S, L Abdullah, D Diapari, I E Saputra, J Ramadani

This work is licensed under a Creative Commons Attribution 4.0 International License.







