Performance, Egg Quality, Bone Health, and Immunity Assessments of Lohmann Laying Hens Supplemented with Vitamin D3 in the Diet
Abstract
Bone health in laying hens is associated with production performance and physical egg quality. This study aimed to evaluate the effects of a diet supplemented with Nutricell Eggstra (vitamin D3 enriched with canthaxanthin) on the production performance, egg quality, bone mineral, and immunity of laying hens. Herein, 150 Lohmann laying hens were divided into three treatments with five replicates. The treatment diets were: T1= diet without Nutricell Eggstra supplementation (control), T2= diet supplemented with 1.00 kg/ton Nutricell Eggstra, and T3= diet supplemented with 1.50 kg/ton Nutricell Eggstra. Compared to the control diet, supplementation of Nutricell Eggstra at doses of 1.00 kg/ton and 1.50 kg/ton significantly increased (p<0.05) hen day production, egg mass, egg index, yolk index, albumen weight, yolk color, eggshell thickness, eggshell strength, vitamin D3 content in egg yolk, heterophil, and heterophil/lymphocyte ratio. Supplementation of Nutricell Eggstra at the studied doses significantly decreased (p<0.05) the feed conversion ratio, yolk weight, eggshell porosity, and lymphocyte content. Nutricell Eggstra supplementation did not affect feed consumption, egg weight, albumen index, albumen weight, eggshell weight, Haugh unit, and tibia bone minerals. In conclusion, supplementation of 1.00 kg/ton Nutricell Eggstra yielded the best performance for Lohmann laying hens aged 36-39 weeks, while supplementation of 1.50 kg/ton Nutricell Eggstra produced the best quality eggs.
References
Adhikari, R., D. White, J. D. House, & W. K. Kim. 2020. Effects of additional dosage of vitamin D3, vitamin D2, and 25-hydroxyvitamin D3 on calcium and phosphorus utilization, egg quality and bone mineralization in laying hens. Poult. Sci. 99:364-373. https://doi.org/10.3382/ps/pez502
Afandi, R., B. Hartono, & I. Djunaidi. 2020. The analysis of production costs of laying hen farms using semi self-mixing and total self-mixing feeds in Blitar regency, east Java. Trop. Anim. Sci. J. 43:70-76. https://doi.org/10.5398/tasj.2020.43.1.70
AOAC. 2005. Official Methods of Analysis of AOAC International 18th (Ed). International AOAC, Washington DC.
Attia, Y. A., M. A. Al-Harthi, & H. M. Abo El-Maaty. 2020. Calcium and cholecalciferol levels in late-phase laying hens: effects on productive traits, egg quality, blood biochemistry, and immune responses. Front. Vet. Sci. 7:389. https://doi.org/10.3389/fvets.2020.00389
Aygun, A. 2014. The relationship between eggshell colour and egg quality traits in table eggs. Indian J. Anim. Res. 48:290-294. https://doi.org/10.5958/j.0976-0555.48.3.061
Boodhoo, N., S. Sharif, & S. Behboudi. 2016. 1α,25(OH)2 vitamin D3 modulates Avian T lymphocyte functions without inducing CTL unresponsiveness. PLoS ONE. 11:e0150134. https://doi.org/10.1371/journal.pone.0150134
BSN. 2008. Telur Ayam Konsumsi SNI-3926:2008. Badan Standardisasi Nasional, Jakarta (ID).
Chen, C., B. Turner, T. J. Applegat, G. Litta, & W. K Kim. 2020. Role of long-term supplementation of 25-hydroxyvitamin D3 on laying hen bone 3-dimensional structural development. Poult. Sci. 99:5771-5782. https://doi.org/10.1016/j.psj.2020.06.080
Duran, M. R., C. Chen, & W. K. Kim. 2018. Effects of vitamin D and calcium for the prevention of osteoporosis at various stages of life of laying hens-review. Int. J. Poult. Sci. 17: 405-409. https://doi.org/10.3923/ijps.2018.405.409
EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). 2014. Scientific opinion on the safety and efficacy of canthaxanthin as a feed additive for poultry and for ornamental birds and ornamental fish. EFSA J. 12:3527. https://doi.org/10.2903/j.efsa.2014.3527
El-Sabrout, K., S. Aggag, & B. Mishra. 2022. Advanced practical strategies to enhance table egg production. Scientifica 17. https://doi.org/10.1155/2022/1393392
Faruk, M. U., F. F. Roos, & F. Cisneros-Gonzalez. 2018. A meta-analysis on the effect of canthaxanthin on egg production in brown egg layers. Poult. Sci. 97:84-87. https://doi.org/10.3382/ps/pex236
Food and Agriculture Organization of the United Nation. 2022. World Food and Agriculture. FAO Statistics, Rome.
Fritts, C. A. & P. W. Waldroup. 2003. Effects of source and level of vitamin D on live performance and bone development in growing broilers. J. Appl. Poult. Res. 12:45-52. https://doi.org/10.1093/japr/12.1.45
Garcia, A. F., A. E. Murakami, C. R Duarte, I. C. Rojas, K. P. Picoli, & M. M Puzotti. 2013. Use of vitamin D3 and its metabolites in broiler chicken feed on performance, bone parameters, meat quality. Asian-Australas. J. Anim. Sci. 26: 408-415. https://doi.org/10.5713/ajas.2012.12455
Geng, Y., Q. Ma, Z. Wang, & Y. Guo. 2018. Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress. Nutr. Metab. 15:1-14. https://doi.org/10.1186/s12986-018-0293-8
Gole, V. C., K. K. Chousalkar, J. R. Roberts, M. Sexton, D. May, J. Tan, & A. Kiermeier. 2014. Effect of egg washing and correlation between eggshell characteristics and egg penetration by various Salmonella Typhimurium strains. PLoS ONE 9:e90987. https://doi.org/10.1371/journal.pone.0090987
Groff, J. L. & S. S. Gropper. 2000. The Digestive System: Mechanism for Nourishing the Body. Advanced Nutrition and Human Metabolism. 3rd ed. Wadsworth Thomson Learning, Belmont (CA). pp. 24-52.
Gross, W. B. & H. S. Siegel. 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27:972-979. https://doi.org/10.2307/1590198
Guo, F., Y. Geng, W. Abbas, W. Zhen, S. Wang, Y. Huang, Y. Guo, Q. Ma, & Z. Wang. 2022. Vitamin D3 nutritional status affects gut health of Salmonella-challenged laying hens. Front. Nutr. 9:888580. https://doi.org/10.3389/fnut.2022.888580
Haugh, R. R. 1937. A new method for determining the quality of an egg. US Poultry Magazine 39:27-49.
Hofmann, T., S. S. Schmucker, W. Bessei, M. Grashorn, & V. Stefanski. 2020. Impact of housing environment on the immune system in chickens: a review. Animals 10. https://doi.org/10.3390/ani10071138
Hu, J., Y. Xiong, R. S. Gates, & H. W. Cheng. 2021. Perches as cooling devices for reducing heat stress in caged laying hens: a review. Animals (Basel). 11:3026. https://doi.org/10.3390/ani11113026
Kakhki, R. A. M., T. Heuthorst, A. Wornath-Vanhumbeck, M. Neijat, & E. Kiarie. 2019. Medullary bone attributes in aged Lohmann LSL-lite layers fed different levels of calcium and top-dressed 25-hydroxy vitamin D3. Canadian J. Anim. Sci. 99:138-149. https://doi.org/10.1139/cjas-2018-0062
Kaur, R., B. M. Rathgeber, K. L. Thompson, & J. MacIsaac. 2013. Uterine fluid proteins and egg quality characteristics for 2 commercial and 2 heritage laying hen lines in response to manipulation of dietary calcium and vitamin D3. Poult. Sci. 92: 2419-2432. https://doi.org/10.3382/ps.2012-02983
Kerschnitzki, M., T. Zander, P. Zaslansky, P. Fratzl, R. Shahar, & W. Wagermaier. 2014. Rapid alterations of avian medullary bone material during the daily egg-laying cycle. Bone 69:109–117. https://doi.org/10.1016/j.bone.2014.08.019
Khan, M. J. A., S. H. Khan, A. Bukhsh, M. I. Abbass, & M. Javed. 2016. Effect of different storage period on egg weight, internal egg quality and hatchability characteristics of fayumi eggs. Italian J. Anim. Sci. 12:51. https://doi.org/10.4081/ijas.2013.e51
Ko, K. H., I. K. Lee, G. Kim, M. J. Gu, H. Y. Kim, B. Park, T. S. Park, S. H. Han, & C. H. Yun. 2018. Changes in bursal B cells in chicken during embryonic development and early life after hatching. Sci Rep. 8:16905. https://doi.org/10.1038/s41598-018-34897-4
Lan, R. X., S. I. Lee, & I. H. Kim. 2017. Effects of Enterococcus faecium SLB 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. Poult. Sci. 96:3246-3253. https://doi.org/10.3382/ps/pex101
Leeson, S. & J. D. Summers. 2005. Commercial Poultry Nutrition. 3rd ed. Nottingham Univ Pr., Nottingham (UK).
Li, D., K. Zhang, S. Bai, J. Wang, Q. Zeng, H. Peng, Z. Su, Y. Xuan, S. Qi, & X. Ding. 2021. Effect of 25-hydroxycholecalciferol with different vitamin D3 levels in the hens diet in the rearing period on growth performance, bone quality, egg production, and eggshell quality. Agriculture 11:698. https://doi.org/10.3390/agriculture11080698
Lohmann Management Guide. 2019. Lohmann Brown Lite Layer Management Guide Cage Housing. Lohmann Tierzucht.
Martens, P. J., C. Gysemans, A. Verstuyf, & A. C. Mathieu. 2020. Vitamin D’s effect on immune function. Nutrients 12:1248. https://doi.org/10.3390/nu12051248
Muras, A., M. Romero, C. Mayer, & A. Otero. 2021. Biotechnological applications of Bacillus licheniformis. Crit Rev Biotechnol. 41:609–627. https://doi.org/10.1080/07388551.2021.1873239
Nabi, F., M. A. Arain, N. Rajput, M. Alagawany, J. Soomro, M. Umer, F. Soomro, Z. Wang, R. Ye, & J. Liu. 2020. Health benefits of carotenoids and potential application in poultry industry: a review. J. Anim. Physiol. Anim. Nutr. 104:1809–1818. https://doi.org/10.1111/jpn.13375
Nascimento, G. R., A. E. Murakami, A. F. Q. M. Guerra, I. C. Ospinas-Rojas, M. F. Z. Ferreira, & J. C. Fanhani. 2014. Effect of different vitamin D sources & calcium levels in the diet of lay in the second laying cycle. Braz. J. Poult. Sci. 16: 37-42. https://doi.org/10.1590/1516-635x160237-42
North, M. O. & D. D. Bell. 1990. Commercial Chicken Production Manual. Springer, New York.
Obianwuna, U. E., V. U. O. Okoleh, J. Wang, H. Zhang, G. Qi, K. Qiu, & S. G. Wu. 2022. Natural products of plants and animal origin improve albumen quality of chicken eggs. Frontiers in Nutrition. 9. https://doi.org/10.3389/fnut.2022.875270
Owen, J. A., J. Punt, & S. A Stranford. 2013. KUBY: Immunology. 7th Ed. Macmillan Higher Education, Basingstoke (UK).
Pakpahan, A. M. B., Y. Retnani, R. Mutia, & W. W. Wardani. 2023. Evaluation of the physical quality of supplement wafer and application of supplement on layer performance. Buletin Peternakan 47:190–197. http://dx.doi.org/10.21059/buletinpeternak.v47i3.84653
Pantaya, D. & M. M. D. Utami. 2020. The blood haematological profile on laying hens that treated by different levels of yeast supplementation. IOP Conf. Ser.: Earth Environ. Sci. 207:012033. https://doi.org/10.1088/1755-1315/207/1/012033
Plaimast, H., S. Kijparkorn, & P. Ittitanawong. 2015. Effects of vitamin D3 and calcium on productive performance, egg quality and vitamin D3 content in egg of second production cycle fens. Thai J. Vet. Med. 45: 189–195. https://doi.org/10.56808/2985-1130.2635
Salles, J., A. Chanet, C. Giraudet, V. Patrac, P. Pierre, M. Jourdan, Y. C. Luiking, S. Verlaan, C.Migné, Y. Boirie, & S. Walrand. 2013. 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C 2 C 12 skeletal myotubes. Mol. Nutr. Food Res. 57:2137–2146. https://doi.org/10.1002/mnfr.201300074
Samiullah, S., J. R. Roberts, & K. Chousalkar. 2015. Eggshell color in brown-egg laying hens - a review. Poult. Sci. 94:2566-2575. https://doi.org/10.3382/ps/pev202
Saunders-Blades, J. L. & D. R. Korver. 2014. The effect of maternal vitamin D source on broiler hatching egg quality, hatchability, and progeny bone mineral density and performance. J. Appl. Poult. Sci. 23:773–783. https://doi.org/10.3382/japr.2014-01006
Schalm, O. W. 1961. Veterinary Hematology. Lea & Febiger, Philadelphia, US.
Schisler, D. K. & E. W Kienholz. 1967. Interaction of dietary zinc and vitamin D in laying hens. Poult. Sci. 46:918-924. https://doi.org/10.3382/ps.0460918
Schneider, W. J. 2016. Lipid transport to avian oocytes and to the developing embryo. J. Biomed. Res. 30:174-180. https://doi.org/10.7555/JBR.30.20150048
Setiyaningsih, N., Sumiati, A. Jayanegara, & W. W. Wardani. 2022. Evaluation of Nutricell HyC® (combination of 25OHD3 and vitamin C) supplementation on broiler breeders fertility in laying period. IOP Conference Series: Earth and Environmental Science. 1020(1). https://doi.org/10.1088/1755-1315/1020/1/012016
Setiyaningsih, N., Sumiati, A. Jayanegara, & W. W. Wardani. 2023. Effects of a vitamins D and C supplement on performance, hatchability and blood profiles of broiler breeders. J. World Poult. Res. 13:71-80. https://dx.doi.org/10.36380/jwpr.2023.7
Shojadoost, B., S. Behboudi, A. I. Villanueva, J. T. Brisbin, A. A. Ashkar, & S. Sharif. 2015. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 100:45–51. https://doi.org/10.1016/j.rvsc.2015.03.009
Sinclair-Black, M., R. A. Garcia, & L. E. Ellestad. 2023. Physiological regulation of calcium and phosphorus utilization in laying hens. Front. Physiol. 14:1112499. https://doi.org/10.3389/fphys.2023.1112499
USDA. 2000. Egg Grading Manual Handbook. Quorn. Foods Inc., Washington DC (US).
Wang, J., L. Qiu, H. Gong, P. Celi, L. Yan, X. Ding, S. Bai, Q. Zeng, X. Mao, S. Xu, C. Wu, & K. Zhang. 2020. Effect of dietary 25-hydroxycholecalciferol supplementation and high stocking density on performance, egg quality, and tibia quality in laying hens. Poult. Sci. 99:2608-2615. https://doi.org/10.1016/j.psj.2019.12.054
Wang, X., P. Zhu, Z. Sun, J. Zhang, & C. Sun. 2021. Uterine metabolomic analysis for the regulation of eggshell calcification in chickens. Metabolites 11:575. https://doi.org/10.3390/metabo11090575
Wen, J., K. A. Livingston, & M. E Persia. 2019. Effect of high concentrations of dietary vitamin D3 on pullet and laying hen performance, skeleton health, eggshell quality, and yolk vitamin D3 content when fed to W36 laying hens from day of hatch until 68 wk of age. Poult. Sci. 98:6713–6720. https://doi.org/10.3382/ps/pez386
Yan, L., M. Hu, L. Gu, M. Lei, Z. Chen, H. Zhu, & R. Chen. 2022. Effect of heat stress on egg production, steroid hormone synthesis, and related gene expression in chicken preovulatory follicular granulosa cells. Animals (Basel) 12:1467. https://doi.org/10.3390/ani12111467
Yao, L., T. Wang, M. Persia, R. L. Horst, & M. Higgins. 2013. Effects of vitamin D3-enriched diet on egg yolk vitamin D3 content and yolk quality. J. Food Sci. 78:178-183. https://doi.org/10.1111/1750-3841.12032
Zhang, W., K. Y. Zhang, X. M. Ding, S. P. Bai, J. M. Hernande, B. Yao, & Q. Zhu. 2011. Influence of canthaxanthin on broiler breeder reproduction, chick quality, and performance. Poult. Sci. 90:1516–1522. https://doi.org/10.3382/ps.2010-01126
Zhen, W., Y. Shao, X. Gong, Y. Wu, Y. Geng, Z. Wang, & Y. Guo. 2018. Effect of dietary Bacillus coagulans supplementation on growth performance and immune responses of broiler chickens challenged by Salmonella enteritidis. Poult. Sci. 97: 2654–2666. https://doi.org/10.3382/ps/pey119
Afandi, R., B. Hartono, & I. Djunaidi. 2020. The analysis of production costs of laying hen farms using semi self-mixing and total self-mixing feeds in Blitar regency, east Java. Trop. Anim. Sci. J. 43:70-76. https://doi.org/10.5398/tasj.2020.43.1.70
AOAC. 2005. Official Methods of Analysis of AOAC International 18th (Ed). International AOAC, Washington DC.
Attia, Y. A., M. A. Al-Harthi, & H. M. Abo El-Maaty. 2020. Calcium and cholecalciferol levels in late-phase laying hens: effects on productive traits, egg quality, blood biochemistry, and immune responses. Front. Vet. Sci. 7:389. https://doi.org/10.3389/fvets.2020.00389
Aygun, A. 2014. The relationship between eggshell colour and egg quality traits in table eggs. Indian J. Anim. Res. 48:290-294. https://doi.org/10.5958/j.0976-0555.48.3.061
Boodhoo, N., S. Sharif, & S. Behboudi. 2016. 1α,25(OH)2 vitamin D3 modulates Avian T lymphocyte functions without inducing CTL unresponsiveness. PLoS ONE. 11:e0150134. https://doi.org/10.1371/journal.pone.0150134
BSN. 2008. Telur Ayam Konsumsi SNI-3926:2008. Badan Standardisasi Nasional, Jakarta (ID).
Chen, C., B. Turner, T. J. Applegat, G. Litta, & W. K Kim. 2020. Role of long-term supplementation of 25-hydroxyvitamin D3 on laying hen bone 3-dimensional structural development. Poult. Sci. 99:5771-5782. https://doi.org/10.1016/j.psj.2020.06.080
Duran, M. R., C. Chen, & W. K. Kim. 2018. Effects of vitamin D and calcium for the prevention of osteoporosis at various stages of life of laying hens-review. Int. J. Poult. Sci. 17: 405-409. https://doi.org/10.3923/ijps.2018.405.409
EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed). 2014. Scientific opinion on the safety and efficacy of canthaxanthin as a feed additive for poultry and for ornamental birds and ornamental fish. EFSA J. 12:3527. https://doi.org/10.2903/j.efsa.2014.3527
El-Sabrout, K., S. Aggag, & B. Mishra. 2022. Advanced practical strategies to enhance table egg production. Scientifica 17. https://doi.org/10.1155/2022/1393392
Faruk, M. U., F. F. Roos, & F. Cisneros-Gonzalez. 2018. A meta-analysis on the effect of canthaxanthin on egg production in brown egg layers. Poult. Sci. 97:84-87. https://doi.org/10.3382/ps/pex236
Food and Agriculture Organization of the United Nation. 2022. World Food and Agriculture. FAO Statistics, Rome.
Fritts, C. A. & P. W. Waldroup. 2003. Effects of source and level of vitamin D on live performance and bone development in growing broilers. J. Appl. Poult. Res. 12:45-52. https://doi.org/10.1093/japr/12.1.45
Garcia, A. F., A. E. Murakami, C. R Duarte, I. C. Rojas, K. P. Picoli, & M. M Puzotti. 2013. Use of vitamin D3 and its metabolites in broiler chicken feed on performance, bone parameters, meat quality. Asian-Australas. J. Anim. Sci. 26: 408-415. https://doi.org/10.5713/ajas.2012.12455
Geng, Y., Q. Ma, Z. Wang, & Y. Guo. 2018. Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress. Nutr. Metab. 15:1-14. https://doi.org/10.1186/s12986-018-0293-8
Gole, V. C., K. K. Chousalkar, J. R. Roberts, M. Sexton, D. May, J. Tan, & A. Kiermeier. 2014. Effect of egg washing and correlation between eggshell characteristics and egg penetration by various Salmonella Typhimurium strains. PLoS ONE 9:e90987. https://doi.org/10.1371/journal.pone.0090987
Groff, J. L. & S. S. Gropper. 2000. The Digestive System: Mechanism for Nourishing the Body. Advanced Nutrition and Human Metabolism. 3rd ed. Wadsworth Thomson Learning, Belmont (CA). pp. 24-52.
Gross, W. B. & H. S. Siegel. 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27:972-979. https://doi.org/10.2307/1590198
Guo, F., Y. Geng, W. Abbas, W. Zhen, S. Wang, Y. Huang, Y. Guo, Q. Ma, & Z. Wang. 2022. Vitamin D3 nutritional status affects gut health of Salmonella-challenged laying hens. Front. Nutr. 9:888580. https://doi.org/10.3389/fnut.2022.888580
Haugh, R. R. 1937. A new method for determining the quality of an egg. US Poultry Magazine 39:27-49.
Hofmann, T., S. S. Schmucker, W. Bessei, M. Grashorn, & V. Stefanski. 2020. Impact of housing environment on the immune system in chickens: a review. Animals 10. https://doi.org/10.3390/ani10071138
Hu, J., Y. Xiong, R. S. Gates, & H. W. Cheng. 2021. Perches as cooling devices for reducing heat stress in caged laying hens: a review. Animals (Basel). 11:3026. https://doi.org/10.3390/ani11113026
Kakhki, R. A. M., T. Heuthorst, A. Wornath-Vanhumbeck, M. Neijat, & E. Kiarie. 2019. Medullary bone attributes in aged Lohmann LSL-lite layers fed different levels of calcium and top-dressed 25-hydroxy vitamin D3. Canadian J. Anim. Sci. 99:138-149. https://doi.org/10.1139/cjas-2018-0062
Kaur, R., B. M. Rathgeber, K. L. Thompson, & J. MacIsaac. 2013. Uterine fluid proteins and egg quality characteristics for 2 commercial and 2 heritage laying hen lines in response to manipulation of dietary calcium and vitamin D3. Poult. Sci. 92: 2419-2432. https://doi.org/10.3382/ps.2012-02983
Kerschnitzki, M., T. Zander, P. Zaslansky, P. Fratzl, R. Shahar, & W. Wagermaier. 2014. Rapid alterations of avian medullary bone material during the daily egg-laying cycle. Bone 69:109–117. https://doi.org/10.1016/j.bone.2014.08.019
Khan, M. J. A., S. H. Khan, A. Bukhsh, M. I. Abbass, & M. Javed. 2016. Effect of different storage period on egg weight, internal egg quality and hatchability characteristics of fayumi eggs. Italian J. Anim. Sci. 12:51. https://doi.org/10.4081/ijas.2013.e51
Ko, K. H., I. K. Lee, G. Kim, M. J. Gu, H. Y. Kim, B. Park, T. S. Park, S. H. Han, & C. H. Yun. 2018. Changes in bursal B cells in chicken during embryonic development and early life after hatching. Sci Rep. 8:16905. https://doi.org/10.1038/s41598-018-34897-4
Lan, R. X., S. I. Lee, & I. H. Kim. 2017. Effects of Enterococcus faecium SLB 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. Poult. Sci. 96:3246-3253. https://doi.org/10.3382/ps/pex101
Leeson, S. & J. D. Summers. 2005. Commercial Poultry Nutrition. 3rd ed. Nottingham Univ Pr., Nottingham (UK).
Li, D., K. Zhang, S. Bai, J. Wang, Q. Zeng, H. Peng, Z. Su, Y. Xuan, S. Qi, & X. Ding. 2021. Effect of 25-hydroxycholecalciferol with different vitamin D3 levels in the hens diet in the rearing period on growth performance, bone quality, egg production, and eggshell quality. Agriculture 11:698. https://doi.org/10.3390/agriculture11080698
Lohmann Management Guide. 2019. Lohmann Brown Lite Layer Management Guide Cage Housing. Lohmann Tierzucht.
Martens, P. J., C. Gysemans, A. Verstuyf, & A. C. Mathieu. 2020. Vitamin D’s effect on immune function. Nutrients 12:1248. https://doi.org/10.3390/nu12051248
Muras, A., M. Romero, C. Mayer, & A. Otero. 2021. Biotechnological applications of Bacillus licheniformis. Crit Rev Biotechnol. 41:609–627. https://doi.org/10.1080/07388551.2021.1873239
Nabi, F., M. A. Arain, N. Rajput, M. Alagawany, J. Soomro, M. Umer, F. Soomro, Z. Wang, R. Ye, & J. Liu. 2020. Health benefits of carotenoids and potential application in poultry industry: a review. J. Anim. Physiol. Anim. Nutr. 104:1809–1818. https://doi.org/10.1111/jpn.13375
Nascimento, G. R., A. E. Murakami, A. F. Q. M. Guerra, I. C. Ospinas-Rojas, M. F. Z. Ferreira, & J. C. Fanhani. 2014. Effect of different vitamin D sources & calcium levels in the diet of lay in the second laying cycle. Braz. J. Poult. Sci. 16: 37-42. https://doi.org/10.1590/1516-635x160237-42
North, M. O. & D. D. Bell. 1990. Commercial Chicken Production Manual. Springer, New York.
Obianwuna, U. E., V. U. O. Okoleh, J. Wang, H. Zhang, G. Qi, K. Qiu, & S. G. Wu. 2022. Natural products of plants and animal origin improve albumen quality of chicken eggs. Frontiers in Nutrition. 9. https://doi.org/10.3389/fnut.2022.875270
Owen, J. A., J. Punt, & S. A Stranford. 2013. KUBY: Immunology. 7th Ed. Macmillan Higher Education, Basingstoke (UK).
Pakpahan, A. M. B., Y. Retnani, R. Mutia, & W. W. Wardani. 2023. Evaluation of the physical quality of supplement wafer and application of supplement on layer performance. Buletin Peternakan 47:190–197. http://dx.doi.org/10.21059/buletinpeternak.v47i3.84653
Pantaya, D. & M. M. D. Utami. 2020. The blood haematological profile on laying hens that treated by different levels of yeast supplementation. IOP Conf. Ser.: Earth Environ. Sci. 207:012033. https://doi.org/10.1088/1755-1315/207/1/012033
Plaimast, H., S. Kijparkorn, & P. Ittitanawong. 2015. Effects of vitamin D3 and calcium on productive performance, egg quality and vitamin D3 content in egg of second production cycle fens. Thai J. Vet. Med. 45: 189–195. https://doi.org/10.56808/2985-1130.2635
Salles, J., A. Chanet, C. Giraudet, V. Patrac, P. Pierre, M. Jourdan, Y. C. Luiking, S. Verlaan, C.Migné, Y. Boirie, & S. Walrand. 2013. 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C 2 C 12 skeletal myotubes. Mol. Nutr. Food Res. 57:2137–2146. https://doi.org/10.1002/mnfr.201300074
Samiullah, S., J. R. Roberts, & K. Chousalkar. 2015. Eggshell color in brown-egg laying hens - a review. Poult. Sci. 94:2566-2575. https://doi.org/10.3382/ps/pev202
Saunders-Blades, J. L. & D. R. Korver. 2014. The effect of maternal vitamin D source on broiler hatching egg quality, hatchability, and progeny bone mineral density and performance. J. Appl. Poult. Sci. 23:773–783. https://doi.org/10.3382/japr.2014-01006
Schalm, O. W. 1961. Veterinary Hematology. Lea & Febiger, Philadelphia, US.
Schisler, D. K. & E. W Kienholz. 1967. Interaction of dietary zinc and vitamin D in laying hens. Poult. Sci. 46:918-924. https://doi.org/10.3382/ps.0460918
Schneider, W. J. 2016. Lipid transport to avian oocytes and to the developing embryo. J. Biomed. Res. 30:174-180. https://doi.org/10.7555/JBR.30.20150048
Setiyaningsih, N., Sumiati, A. Jayanegara, & W. W. Wardani. 2022. Evaluation of Nutricell HyC® (combination of 25OHD3 and vitamin C) supplementation on broiler breeders fertility in laying period. IOP Conference Series: Earth and Environmental Science. 1020(1). https://doi.org/10.1088/1755-1315/1020/1/012016
Setiyaningsih, N., Sumiati, A. Jayanegara, & W. W. Wardani. 2023. Effects of a vitamins D and C supplement on performance, hatchability and blood profiles of broiler breeders. J. World Poult. Res. 13:71-80. https://dx.doi.org/10.36380/jwpr.2023.7
Shojadoost, B., S. Behboudi, A. I. Villanueva, J. T. Brisbin, A. A. Ashkar, & S. Sharif. 2015. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 100:45–51. https://doi.org/10.1016/j.rvsc.2015.03.009
Sinclair-Black, M., R. A. Garcia, & L. E. Ellestad. 2023. Physiological regulation of calcium and phosphorus utilization in laying hens. Front. Physiol. 14:1112499. https://doi.org/10.3389/fphys.2023.1112499
USDA. 2000. Egg Grading Manual Handbook. Quorn. Foods Inc., Washington DC (US).
Wang, J., L. Qiu, H. Gong, P. Celi, L. Yan, X. Ding, S. Bai, Q. Zeng, X. Mao, S. Xu, C. Wu, & K. Zhang. 2020. Effect of dietary 25-hydroxycholecalciferol supplementation and high stocking density on performance, egg quality, and tibia quality in laying hens. Poult. Sci. 99:2608-2615. https://doi.org/10.1016/j.psj.2019.12.054
Wang, X., P. Zhu, Z. Sun, J. Zhang, & C. Sun. 2021. Uterine metabolomic analysis for the regulation of eggshell calcification in chickens. Metabolites 11:575. https://doi.org/10.3390/metabo11090575
Wen, J., K. A. Livingston, & M. E Persia. 2019. Effect of high concentrations of dietary vitamin D3 on pullet and laying hen performance, skeleton health, eggshell quality, and yolk vitamin D3 content when fed to W36 laying hens from day of hatch until 68 wk of age. Poult. Sci. 98:6713–6720. https://doi.org/10.3382/ps/pez386
Yan, L., M. Hu, L. Gu, M. Lei, Z. Chen, H. Zhu, & R. Chen. 2022. Effect of heat stress on egg production, steroid hormone synthesis, and related gene expression in chicken preovulatory follicular granulosa cells. Animals (Basel) 12:1467. https://doi.org/10.3390/ani12111467
Yao, L., T. Wang, M. Persia, R. L. Horst, & M. Higgins. 2013. Effects of vitamin D3-enriched diet on egg yolk vitamin D3 content and yolk quality. J. Food Sci. 78:178-183. https://doi.org/10.1111/1750-3841.12032
Zhang, W., K. Y. Zhang, X. M. Ding, S. P. Bai, J. M. Hernande, B. Yao, & Q. Zhu. 2011. Influence of canthaxanthin on broiler breeder reproduction, chick quality, and performance. Poult. Sci. 90:1516–1522. https://doi.org/10.3382/ps.2010-01126
Zhen, W., Y. Shao, X. Gong, Y. Wu, Y. Geng, Z. Wang, & Y. Guo. 2018. Effect of dietary Bacillus coagulans supplementation on growth performance and immune responses of broiler chickens challenged by Salmonella enteritidis. Poult. Sci. 97: 2654–2666. https://doi.org/10.3382/ps/pey119
Authors
YusufG. M., Sumiati, MutiaR., WardaniW. W., AkbarI., & PutriN. D. S. (2023). Performance, Egg Quality, Bone Health, and Immunity Assessments of Lohmann Laying Hens Supplemented with Vitamin D3 in the Diet. Tropical Animal Science Journal, 46(4), 461-470. https://doi.org/10.5398/tasj.2023.46.4.461
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