Preparation of Active Food Packaging and Coating Material Based on Bacterial Cellulose to Increase Food Safety
Abstract
The use of bacterial probiotic metabolite-based active-packaging and coatings is an innovative approach that has gained widespread attention worldwide. Additionally, its utilization can lead to improvements in qualities and properties of food products. This study was aimed to develop a food spoilage prevention system using active food packaging and coating material in preventing food spoilage while increasing its shelflife. The materials used were bacterial cellulose (BC) based bioplastics fortified with fermented soymilk extracts (FSME) using Lactobacillus acidophilus as the producer of the antimicrobial and antioxidant agents. Moreover, the applications of FSME containing probiotic bacterial metabolites are discussed to highlight their efficacy in enhancing the quality and shelf life of food products.The antimicrobial test showed that the FSME could inhibit the growth of pathogenic microbial cultures at minimum inhibitory concentration (MIC) of 10% (v/v) as shown by clear zones, around colonies of E. coli (14.33±0.58 mm), S. aureus (18.33±6.03 mm), S. Typhimurium (11.67±1.15 mm), L. monocytogenes (11.33±2.31 mm), and B. cereus (13.33±3.06 mm). Meanwhile the results of IC50 for antioxidant activity test (µg/mL) indicated that the FSME showed radical scavenging activity against DPPH at approximately 75.27±2.552 (2.5%, v/v), 55.00±0.791 (5.0%, v/v), 43.17±1.603 (7.5%, v/v) and 15.05±0.346 (10%, v/v), respectively. The shelflife of strawberries coated with the active food coating using the bioplastic fortified with FSME showed an increase in shelf life of 14 days at 4°C. The overall results indicated that the use of BC based bioplastics fortified with FSME can play an important role in preventing premature spoilage and increasing the shelf life of food products.
References
Abe MM, Martins JR, Sanvezzo PB, Macedo JV, Branciforti MC, Halley P, Botaro VR, Brienzo M. 2021. Advantages and disadvantages of bioplastics production from starch and lignocellulosic components. Polymers 13: 2484. https://doi.org/10.3390/polym13152484
Abral H, Putra GJ, Asrofi M, Park J-W, Kim H-J. 2018. Effect of vibration duration of high ultrasound applied to biocomposite while gelatinized on its properties. Ultrason Sonochem 40 Part A: 697-702. https://doi.org/10.1016/j.ultsonch.2017.08.019
Ahmed I, Lin H, Zou L, Brody AL, Li Z, Qazi IM, Pavase TR, Lv L. 2017. A comprehensive review on the application of active packaging technologies to muscle foods. Food Control 82: 163-178. https://doi.org/10.1016/j.foodcont.2017.06.009
Almalki MA. 2020. Exopolysaccharide production by a new Lactobacillus lactis isolated from the fermented milk and its antioxidant properties. J. King Saud Univ Sci 32: 1272-1277. https://doi.org/10.1016/j.jksus.2019.11.002
Arasu MV, Kim DH, Kim PI, Jung MW, Ilavenil S, Jane M, Lee KD, Al-Dhabi NA, Choi KC. 2014. In vitro antifungal, probiotic and antioxidant properties of novel Lactobacillus plantarum K46 isolated from fermented sesame leaf. Ann Microbiol 64: 1333-1346. https://doi.org/10.1007/s13213-013-0777-8
Aykul S, Martinez-Hackert E. 2016. Determination of half-maximal inhibitory concentration using biosensor-based protein interaction analysis. Anal Biochem 508: 97-103. https://doi.org/10.1016/j.ab.2016.06.025
Azarakhsh N, Osman A, Ghazali HM, Tan CP, Adzahan NM. 2014. Effects of gellan-based edible coating on the quality of fresh-cut pineapple during cold storage. Food Bioproc Tech 7: 2144-2151. https://doi.org/10.1007/s11947-014-1261-6
Azeredo HMC, Barud H, Farinas CS, Vasconcellos VM, Claro AM. 2019. Bacterial cellulose as a raw material for food and food packaging applications. Front Sustain Food Syst 3: 1-14. https://doi.org/10.3389/fsufs.2019.00007
Bagheri, L, Madadlou A, Yarmand M, Mousavi ME. 2013. Nanoencapsulation of date palm pit extract in whey protein particles generated via desolvation method. Food Res Int 51: 866-871. https://doi.org/10.1016/j.foodres.2013.01.058
Błaszczyk J, Bieniasz M, Nawrocki J, Kopeć M, Mierzwa-Hersztek M, Gondek K, Zaleski T, Knaga J, Bogdał S. 2022. The effect of harvest date and storage conditions on the quality of remontant strawberry cultivars grown in a gutter. Agric 12: 1193. https://doi.org/10.3390/agriculture12081193
Brachkova MI, Marques P, Rocha J, Sepodes B, Duarte MA, Pinto JF. 2011. Alginate films containing Lactobacillus plantarum as wound dressing for prevention of burn infection. J Hospital Infect 79: 375-377. https://doi.org/10.1016/j.jhin.2011.09.003
Cabañas-Romero LV, Valls C, Valenzuela SV, Roncero MB, Pastor FIJ, Diaz P, Martínez J. 2020. Bacterial cellulose-chitosan paper with antimicrobial and antioxidant activities. Biomacromolecules 21: 1568-1577. https://doi.org/10.1021/acs.biomac.0c00127
Cazón P, Vázquez M. 2020. Mechanical and barrier properties of chitosan combined with other components as food packaging film. Environ Chem Lett 18: 257-267. https://doi.org/10.1007/s10311-019-00936-3
Costa AFS, Almeida FCG, Vinhas GM, Sarubbo LA. 2017. Production of bacterial cellulose by Gluconacetobacter hansenii using corn steep liquor as nutrient sources. Front Microbiol 8: 2027. https://doi.org/10.3389/fmicb.2017.02027
Dinev T, Beev G, Denev S, Dermendzhieva D, Tzanova M, Valkova E. 2017. Antimicrobial activity of Lactobacillus acidophilus against pathogenic and food spoilage microorganisms: A review. Agric Sci Technol 9: 3-9. https://doi.org/10.15547/ast.2017.01.001
Dobrucka R, Przekop R. 2019. New perspectives in active and intelligent food packaging. J Food Process Preserv 43: e14194. https://doi.org/10.1111/jfpp.14194
Espitia PJP, Batista RA, Azeredo HMC, Otoni CG. 2016. Probiotics and their potential applications in active edible films and coatings. Food Res Int 90: 42-52. https://doi.org/10.1016/j.foodres.2016.10.026
Ganiari S, Choulitoudi E, Oreopoulou V. 2017. Edible and active films and coatings as carriers of natural antioxidants for lipid food. Trends Food Sci Technol 68: 70-82. https://doi.org/10.1016/j.tifs.2017.08.009
Gangwar M, Gautam MK, Sharma AK. Tripathi YB, Goel RK, Nath G. 2014. An in vitro study: Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes. Sci World J 2014: 279451. https://doi.org/10.1155/2014/279451
Gol NB, Patel PR, Rao TR. 2013. Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan. Postharvest Biol Technol 85: 185-195. https://doi.org/10.1016/j.postharvbio.2013.06.008
Hsu H-Y, Huang L, Wu JS-B. 2014. Thermal inactivation of Escherichia coli O157:H7 in strawberry puree and its effect on anthocyanins and color. J Food Sci 79: M74-M80. https://doi.org/10.1111/1750-3841.12327
Ibrahim MA, Sharoba AM, El Waseif KH, El Mansy HA, El Tanahy HH. 2017. Effect of edible coating by chitosan with lemongrass and thyme oils on strawberry quality and shelf life during storage. J Food Technol Nutr Sci 3: 1-11.
Jadhav PB, Gurav NP. 2018. Extending the storage and post-storage shelf-life of strawberry fruit cv. “sweet charlie” using cold storage. Res J Agric Sci 9: 532-542.
Kanmani P, Lim ST. 2013. Development and characterization of novel probiotic-residing pullulan/starch edible films. Food Chem 141: 1041-1049. https://doi.org/10.1016/j.foodchem.2013.03.103
Keshk SMAS. 2014. Bacterial cellulose production and its industrial applications. J. Bioprocess Biotech 4: 1-10. https://doi.org/10.4172/2155-9821.1000150
Khodaei D, Hamidi-Esfahani Z, Rahmati E. 2021. Effect of edible coatings on the shelf-life of fresh strawberries: A comparative study using TOPSIS-Shannon entropy method. NFS J 23: 17–23. https://doi.org/10.1016/j.nfs.2021.02.003
Krishnamurthy A, Amritkumar P. 2019. Synthesis and characterization of eco-friendly bioplastic from low-cost plant resources. SN Appl Sci 1: 1432. https://doi.org/10.1007/s42452-019-1460-x
Kumari L, Li WZ, Vannoy CH, Leblanc RM, Wang DZ. 2010. Zinc oxide micro- and nanoparticles: Synthesis, structure and optical properties. Mater Res Bull 45: 190-196. https://doi.org/10.1016/j.materresbull.2009.09.021
Kumar L, Ramakanth D, Akhila K, Gaikwad KK. 2021. Edible films and coatings for food packaging applications: A review. Environ Chem Lett 20: 875-900. https://doi.org/10.1007/s10311-021-01339-z
Lagarón JM, López-Rubio A, Fabra MJ. 2015. Bio-based packaging. J Appl Polym Sci 133: 42971. https://doi.org/10.1002/app.42971
Li Y, Wu C. 2013. Enhanced inactivation of Salmonella Typhimurium from blueberries by combinations of sodium dodecyl sulfate with organic acids or hydrogen peroxide. Food Res Int 54: 1553-1559. https://doi.org/10.1016/j.foodres.2013.09.012
Lin S-P, Calvar IL, Catchmark JM, Liu J-R, Demirci A, Cheng K-C. 2013. Biosynthesis, production and applications of bacterial cellulose. Cellulose 20: 2191-2219. https://doi.org/10.1007/s10570-013-9994-3
Liu CF, Pan TM. 2010. In vitro effects of lactic acid bacteria on cancer cell viability and antioxidant activity. J Food Drug Anal 18: 77-86. https://doi.org/10.38212/2224-6614.2287
Lopez-Rubio A, Gavara R, Lagaron JM. 2006. Bioactive packaging: Turning foods into healthier foods through biomaterials. Trends Food Sci Technol 17: 567-575. https://doi.org/10.1016/j.tifs.2006.04.012
Lubis M, Harahap MB, Ginting MHS, Sebayang AT, Chandra T, Wangi Y, Jose. 2020. Mechanical, SEM and FTIR characteristics of bioplastics from mango seed starch with nanoparticle zinc oxide as filler and ethylene glycol as plasticizers. IOP Conference Series Mater Sci Eng 1003(1): 012122. https://doi.org/10.1088/1757-899X/1003/1/012122
Maqbool M, Ali A, Alderson PG, Mohamed MTM, Siddiqui Y, Zahid N. 2011. Postharvest application of gum arabic and essential oils for controlling anthracnose and quality of banana and papaya during cold storage. Postharvest Biol Technol 62: 71–76. https://doi.org/10.1016/j.postharvbio.2011.04.002
Mohamed SAA, El-Sakhawy M, El-Sakhawy MAM. 2020. Polysaccharides, protein and lipid-based natural edible films in food packaging: A review. Carbohydr Polym 238: 116178. https://doi.org/10.1016/j.carbpol.2020.116178
Morgan BL, Depenbrock S, Martínez-López B. 2022. Identifying associations in minimum inhibitory concentration values of Escherichia coli samples obtained from weaned dairy heifers in California using bayesian network analysis. Front Vet Sci 9: 771841. https://doi.org/10.3389/fvets.2022.771841
Nazir S, Sulistyo J, Ho, AL 2018. Antioxidant properties of polyphenol glycoside catalyzed by transglycosylation reaction of cyclodextrin glucanotransferase derived from Trichoderma viride. J Food Biochem 42: e12499. https://doi.org/10.1111/jfbc.12499
Ncube LK, Ude AU, Ogunmuyiwa EN, Zulkifli R, Beas IN. 2020. Environmental impact of food packaging materials: A review of contemporary development from conventional plastics to polylactic acid based materials. Materials 13: 4994. https://doi.org/10.3390/ma13214994
Noorbakhsh-Soltani SM, Zerafat MM, Sabbaghi S. 2018. A comparative study of gelatin and starch-based nano-composite films modified by nano-cellulose and chitosan for food packaging applications. Carbohydr Polym 189: 48-55. https://doi.org/10.1016/j.carbpol.2018.02.012
Oliveira M, Rodrigues CM, Teixeira P. 2019. Microbiological quality of raw berries and their products: A focus on foodborne pathogens. Heliyon 5: e02992. https://doi.org/10.1016/j.heliyon.2019.e02992
Palumbo M, Harris LJ, Danyluk MD. 2013. Outbreaks of Foodborne Illness Associated with Common Berries, 1983 through May 2013: FSHN13-08/FS232, 11/2013. Edis 2013: 1-9. https://doi.org/10.32473/edis-fs232-2013
Pang M, Huang Y, Meng F, Zhuang Y, Liu H, Du M, Ma Q, Wang Q, Chen Z, Chen L, Cai T, Cai Y. 2020. Application of bacterial cellulose in skin and bone tissue engineering. Eur Polym J 122: 109365. https://doi.org/10.1016/j.eurpolymj.2019.109365
Pavli F, Tassou C, Nychas GJE, Chorianophoulos N. 2018. Probiotic incorporation in edible films and coatings: Bioactive solution for functional foods. Int J Mol Sci 19: 150-167. https://doi.org/10.3390/ijms19010150
Pereira JO, Soares J, Sousa S, Madureira AR, Gomes A, Pintado M. 2016. Edible films as carrier for lactic acid bacteria. LWT 73: 543-550. https://doi.org/10.1016/j.lwt.2016.06.060
Qasim U, Osman AI, Al-Muhtaseb AH, Farrell C, Al-Abri M, Ali M, Vo DVN, Jamil F, Rooney DW. 2021. Renewable cellulosic nanocomposites for food packaging to avoid fossil fuel plastic pollution: A review. Environ Chem Lett 19: 613-641. https://doi.org/10.1007/s10311-020-01090-x
Ramos OL, Reinas I, Silva SI, Fernandes JC, Cerqueira MA, Pereira RN, Vicente AA, Poças MF, Pintado ME, Malcata FX. 2013. Effect of whey protein purity and glycerol content upon physical properties of edible films manufactured therefrom. Food Hydrocolloids 30: 110-122. https://doi.org/10.1016/j.foodhyd.2012.05.001
Rastall RA, Fuller R, Gaskins HR, Gibson GR. 2000. Functional foods. In R. Gibson & C. M. Williams (Eds.), Colonic Functional Foods. 71-95. Cambridge, UK: Woodhead Publishing Limited. https://doi.org/10.1533/9781855736436.2.71
Ribeiro AM, Estevinho BN, Rocha F. 2021. Preparation and incorporation of functional ingredients in edible films and coatings. Food Bioprocess Technol 14: 209-231. https://doi.org/10.1007/s11947-020-02528-4
Salha BA, Gedanken A. 2021. Extending the shelf life of strawberries by the sonochemical coating of their surface with nanoparticles of an edible antibacterial compound. Appl Nano 2: 14-24. https://doi.org/10.3390/applnano2010002
Shahat M, Mohamed MI, Osheba AS, Taha IM. 2020. Improving the quality and shelf-life of strawberries as coated with nano-edible films during storage. Al-Azhar J Agric Res 45: 1-14. https://doi.org/10.21608/ajar.2020.149403
Shekhar CT, Anju G. 2014. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am J Ethnomed 1: 244–249.
Shihabudeen MS, Priscilla DH, Thirumurugan K. 2010. Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plants. Int J Pharm Sci Res 1: 430-434.
Soukoulis C, Behboudi-Jobbehdar S, Macnaughtan W, Parmenter C, Fisk ID. 2017. Stability of Lactobacillus rhamnosus GG incorporated in edible films: impact of anionic biopolymers and whey protein concentrate. Food Hydrocolloids 70: 345-355. https://doi.org/10.1016/j.foodhyd.2017.04.014
Šuput DZ, Lazić VL, Popović SZ, Hromiš NM. 2015. Edible films and coatings - Sources, properties and application. Food Feed Res 42: 11-22. https://doi.org/10.5937/FFR1501011S
Thibessard A, Borges F, Fernandez A, Gintz B, Decaris B, Leblond-Bourget N. 2004, Identification of Streptococcus thermophilus CNRZ368 genes involved in defense against superoxide stress. Appl Environ Microbiol 70: 2220-2229. https://doi.org/10.1128/AEM.70.4.2220-2229.2004
Umaraw P, Munekata PES, Verma AK, Barba FJ, Singh VP, Kumar P, Lorenzo JM. 2020. Edible films/coating with tailored properties for active packaging of meat, fish and derived products. Trends Food Sci Technol 98: 10-24. https://doi.org/10.1016/j.tifs.2020.01.032
Vaishali, Sharma HP, Samsher, Chaudhary V, Sunil, Kumar M. 2019. Importance of edible coating on fruits and vegetables: A review. J Pharmacogn Phytoch 8: 4104-4110.
Velickova E, Winkelhausen E, Kuzmanova S, Alves VD, Moldão-Martins M. 2013. Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (Fragia ananassa cv Camarosa) under commercial storage conditions. LWT-Food Sci Technol 52: 80-92. https://doi.org/10.1016/.lwt.2013.02.004
Vignali G, Vitale G. 2017. Life-Cycle Assessment of Food-Packaging. In Reference Module 669 in Food Science. Elsevier. https://doi.org/10.1016/b978-0-08-100596-5.21427-3
Wicochea-Rodríguez JS, Chalier P, Ruiz T, Gastaldi E. 2019. Active food packaging based on biopolymers and aroma compounds: How to design and control the release. Front Chem 7: 398. https://doi.org/10.3389/fchem.2019.00398
Wu D, Li X, Shen C, Lu J, Chen J, Xie G. 2014. Decreased ethyl carbamate generation during Chinese rice wine fermentation by disruption of CAR1 in an industrial yeast strain. Int J Food Microbiol 180: 19-23. https://doi.org/10.1016/j.ijfoodmicro.2014.04.007
Wojcik M, Burzynska-Pedziwiatr I, Wozniak LA. 2010. A review of natural and synthetic antioxidants important for health and longevity. Curr Med Chem 17: 3262–3288. https://doi.org/10.2174/092986710792231950
Yan J, Luo Z, Ban Z, Lu H, Li D, Yang D, Aghdam MS, Li L. 2019. The of layer-by-layer (LBL) edible coating on strawberry quality and metabolites during storage. Posharvest Biol Technol 147: 29-38. https://doi.org/10.1016/j.postharvbio.2018.09.002
Yildirim S, Röcker B. 2018. Chapter 7: Active Packaging. Nanomaterials for Food Packaging. 173-202. https://doi.org/10.1016/B978-0-323-51271-8.00007-3
Yong H, Liu J. 2021. Active packaging films and edible coatings based on polyphenol-rich propolis extract: A review. Compr Rev Food Sci Food Saf 20: 2106-2145. https://doi.org/10.1111/1541-43 37.12697
Zhong C. 2020. Industrial-Scale production and appli-cations of bacterial cellulose. Front Bioeng Biotechnol 8: 605374. https://doi.org/10.3389/fbioe.2020.605374