Evaluasi penangkapan radikal bebas DPPH ekstrak daun Ipomoea pes-caprae Linn asal Pantai Labuhan Haji, Aceh Selatan The evaluation of DPPH free radical scavenging of Ipomoea pes-caprae leaf from the Beach of Labuhan Haji, South Aceh
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Accepted
7 April 2023
Published
13 August 2023
Keywords:
-
antioxidant, bayhops, dpph, extraction, phenolic compounds
Abstract
Ipomoea pes-caprae plants are herb plants that grow on sandy substrates and are already used by coastal communities as traditional medicine for treating various diseases. This study aimed to evaluate the free radical-scavenging activity of I. pes-caprae leaves from Labuhan Haji Beach, South Aceh. The research included phytochemicals, DPPH free radical scavenging, and total phenolic content. The results showed that I. pes-caprae leaf extracts had the highest yield in methanol extract (6.5%), followed by n-hexane extract (3.5%), and ethyl acetate extract (1.6%). The leaves of I. pes-caprae extract contained alkaloids, phenolics, saponins, flavonoids, and tannins. The free radical scavenging activity shown the methanol extract have IC50 of 13.83±5.85 mg/L whereas ethyl acetate and n-hexane extract accordingly have IC50 of 7.36±1.91 mg/L and 16.01±3.89 mg/L with ascorbate acid have IC50 value of 3.5±1.74 mg/L as the positive control. The total phenolic content in the ethyl acetate extract was 22.9±0.64 mg GAE/g, whereas the methanol extract was 9.89±2.62 mg GAE/g and the lowest total phenolic content in the n-hexane extract with value of 5.90±2.67 mg GAE/g. The phenolic content was positively correlated with antioxidant activity, indicating that the antioxidant activity of I. pes-caprae leaf extracts was attributed to phenolic compounds, whereas the remaining compounds were affected by other compounds contained in the I. pes-caprae leaf extract.
References
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Freeman, B. C., & Beattie, G.A. (2008). An overview of plant defenses against pathogens and herbivores. The Plant Health Instructor. https://doi.org/10.1094/PHI-I-2008-0226-01.
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Kumar, A., Paul, S., Kumari, P., Somasundaram, S. T., & Kathiresan, K. (2014). Antioxidant and free radical scavenging activities of Ipomoea pes-caprae (L.) R. Br. extracts. International Journal of Current Pharmaceutical Research, 5(4), 91-109.
Lattanzio, V., Lattanzio, V. M. T., & Cardinali, A. (2006). Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Advances in Research, 23-67.
Liu, Y., Chen, P., Zhou, M., Wang, T., Fang, S., Shang, X., & Fu, X. (2018). Geographic variation in the chemical composition and antioxidant properties of phenolic compounds from Cyclocarya paliurus (Batal) iljinskaja leaf. Molecules, 23(2440), 1-12. https://doi.org/10.3390/molecules23102440.
Mailandari, M. (2012). Uji aktivitas antioksidan ekstrak daun garcinia kydia roxb dengan metode DPPH dan identifikasi senyawa kimia fraksi yang aktif [Skripsi]. Universitas Indonesia.
Mazid, M., Khan, T. A., & Mohammad, F. (2011). Role of secondary metabolites in defense mechanisms of plants. Biology and Medicine, 3(2), 232-249.
Meria, M., da Silva, E. P., David, J., David, J. M., & David, J. P. (2012). Review of the genus Ipomea: traditional uses, chemistry and biological activities. Brazilian Journal of Pharmacognosy, 22(3), 682-713. https://doi.org/10.1590/S0102-695X2012005000025.
Mohanapriya, S., & Sridevi, J. (2020). Quantification of nutrient and phytoconstituent in fresh and dehydrated Piper betel Leaf. International Journal of Applied Research, 6, 01-04.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219.
Nusch, E. (1980). Comparison of different methods for chlorophyll and phaeopigment determination. Arch Hydrobiol Beih Ergebn Limnology, 14,14-36.
Parekh, K. K., Patel, A. M., Modi, A. J., & Chandrashekhar, H. R. (2012). Antioxidant and cytotoxic activities of few selected Ipomoea species. Pharmacologia, 3(9), 377-386.
Pereda-Miranda., E, Escalante-Sa´nchez., & Escobedo-Martı´nez, C. (2005). Characterization of lipophilic pentasaccharides from beach Morning Glory (Ipomoea pes-caprae). Journal of Natural Products, 68, 226-30. https://doi.org/10.1021/np0496340.
Pourmorad, F., Hosseinimerhr, S. J., & Shahabimajd, N. (2006) Antioxidant activity, phenol and flavonoid contents of some selected iranian medicinal plants. African Journal of Biotechnology, 5, 1142-1145.
Putri S. W. K., Nurhasana, D., Avidlyandi., Gustian, I., Sipriyadi, & Adfa, M. (2021). Aktivitas antibakteri ekstrak etanol daun tapak kuda (Ipomoea pes-caprae (L.) R. Br.) terhadap bakteri Staphylococcus epidermidis. BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains, 4(2), 355-362.
Ratnasooriya, W.D., Pathirana, R. N., Dissanayake, A. S., Samanmali, B. L. C., & Banu, R.S. (2017). Methanolic leaf extract of Ipomoea Pes-caprae possesses in vitro sun screen activity. Imperial Journal of Interdisciplinary Research, 3(2), 150-154.
Rezaeizadeh, A., Zuki, A. B. Z., Abdollahi, M., Goh, Y. M., Noordin, M. M., Hamid, M., & Azmi, T. I. (2011). Determination of antioxidant activity in methanolic and chloroformic extract of momordica charantia. African Journal of Biotechnology, 10(24), 4932-4940.
Robert, P, & Retna A. (2016). 5, 7, 4’ Trihydroxyisoflavone isolated from Ipomea pes-caprae roots by normal phase column chromatography. Bulletin Environment Pharmacology Life Science, 5(5), 27-33.
Sen, D. T., Tan, D. V., & La, H. T. (2013). Biological activity of methanolic extract derived from Ipomea pes-caprae (L.) collected in Xuan Thuy national park. Journal of Science Hnue, 58(9), 139-145.
Shanmugapriya, R., Ramanathan, T., Thirunavukkarasu, P., & Renugadevi, G. (2012). In vitro antifungal activity of Ipomoea pes-caprae (L.) R. Br. ethanolic flower extract. International Journal of Pharmaceutics, 3(1), 732-735.
Teramachi, F., Koyano, T., Kowithayakor, T., Hayashi, M., Komiyama, K., & Ishibashi, M. (2005). Collagenase inhibitory quinic acid ester from Ipomoea pes-caprae. Journal of Natural Products, 68, 794-796. https://doi.org/10.1021/np0500631.
Umamaheshwari, G., Ramanathan, T., & Shanmugapriya, R. (2012). Antioxidant and radical scavenging effect of Ipomea pescaprae Linn.R.BR. International Journal of PharmTech Research, 4(2), 848-851.
Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., & Telser. J. (2007) Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 39, 44–84. https://doi.org/10.1016/j.biocel.2006.07.001.
Venkataraman, N. D., Atlee, W. C., Prabhu, T. P., & Kannan. R. (2013). Anti-inflammatory potential of ethanolic extracts from aerial parts of Ipomoea pes-caprae (L.) RBr using cotton pellet induced granuloma model. Journal of Applied Pharmaceutical Science, 3(7), 61-63. https://doi.org/10.1016/j.jep.2005.07.029.
Vince, O. & Zoltan, M. (2011). Plant Physiology: Secondary metabolites in plant defences. http://www.tankonyvtar.hu/hu/tartalom/tamop425/0010_1A_Book_angol_01_nov enyelettan/ch03s05.html.
Vijayalaxmi, S. K, Jayalakshmi & K Sreeramulu. (2015). Polyphenols from different agricultural residues: extraction, identification and their antioxidant properties. Journal of Food Science and Technology, 52(5), 2761–2769.
Jacoeb, A. M., Suptijah, P., & Zahidah. (2013). Komposisi kimia, komponen bioaktif dan aktivitas antioksidan buah lindur (Bruguiera gymnorrhiza). Jurnal Pengolahan
Hasil Perikanan Indonesia, 16(1), 86-94. https://doi.org/10.17844/jphpi.v16i1.7772.
Widyawati, P. S. (2016). Determination of antioxidant capacity in pluchea indica less leaf extract and its fractions. International Journal of Pharmacy and Pharmaceutical Sciences, 8(9), 32-36. http://dx.doi.org/10.22159/ijpps.2016v8i9.11410.
Wink, M. (2008). Ecological roles of alkaloids. Wink, M. (Eds.) Modern Alkaloids, Structure, Isolation Synthesis and Biology. Wiley-VCH Verlag GmbH & Co. KgaA.
Woo, H., Kuleck, G. A., Hirsch, A. M., & Hawes, M. (2000). The role of flavonoids as signal molecules in plant development. Plant Biology, 11 (12), 2303-2316.
Andayani, D., & Nugrahani, R. (2018). Skrining fitokimia dan aktivitas antioksidan ekstrak etanol daun katang-katang (Ipomoea Pes-caprae. L) dari Pulau Lombok Nusa Tenggara Barat. Journal of Pharmaceutical Science and Clinical Research, 02, 76-83. https://doi.org/10.20961/jpscr.v3i2.21924.
Bhattacharya, A., Sood, P., & Citovsky, V. (2010). The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Molecular plant pathology, 11(5), 705719. https://doi.org/10.1111/j.1364-3703.2010.00625.x.
De Souza, M. M., Madeira, A., Berti, C., Krogh, R., Yunes, R.A., & Filho, V.C. (2000). Antinociceptive properties of the methanolic extract obtained from Ipomoea pes-caprae (L.) R. Br. Journal of Ethnopharmacology, 69(1), 85-90. https://doi.org/10.1016/s0378-8741(99)00142-7.
Escobedo-Martı´nez, & Pereda-Miranda, R. (2007). Resin glycosides from Ipomoea pes-caprae. Journal of Natural Products, 70, 974-8. https://doi.org/10.1021/np070040h.
Freeman, B. C., & Beattie, G.A. (2008). An overview of plant defenses against pathogens and herbivores. The Plant Health Instructor. https://doi.org/10.1094/PHI-I-2008-0226-01.
Ganjir, M., Behera, D. R., & Bhatnagar, S. (2013). Phytochemical analysis, cytotoxic and antioxidant potential of Ipomea pes caprae (L) R. Br and Merremia umbellata (L.) H. Hallier. International Journal of Scientific & Technology Research, 2(5),80-83.
Harborne, J. B. (1987). Metode Fitokimia. Edisi ke-2. Padmawinata K, Soediro I, penerjemah. Bandung: Institut Teknologi Bandung. Terjemahan dari: Phytochemical Methods.
Huliselan, Y. M., Runtuwene, M. R. J., & Wewengkang, D. S. (2015). Aktivitas antioksidan ekstrak etanol, etil asetat, dan n-heksana dari daun sesewanua (Clerondendron squamatum Vahl.). Jurnal Ilmiah Farmasi, 4(3), 155-163. https://doi.org/10.35799/pha.4.2015.8855.
Khamsah, Akowah, & Zhari. (2006). Antioxidant Activity and phenolic content of Orthosiphon stamineus benth from different geographical origin. Journal of Sustainability Science and Management, 1(2), 14-20.
Khopkar, S. M. (2014). Konsep Dasar Kimia Analitik. UI-Press.
Kimball, B. A., & Provenza, F. D. (2003). Chemical defense and mammalian herbivores. USDA National Wildlife Research Center - Staff Publications, 236. Retrieved from http://digital commons.unl. edu/ cgi/viewcontent.cgi?article=1230&context=icwdmusdanwrc.
Kumar, A., Paul, S., Kumari, P., Somasundaram, S. T., & Kathiresan, K. (2014). Antioxidant and free radical scavenging activities of Ipomoea pes-caprae (L.) R. Br. extracts. International Journal of Current Pharmaceutical Research, 5(4), 91-109.
Lattanzio, V., Lattanzio, V. M. T., & Cardinali, A. (2006). Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. Phytochemistry: Advances in Research, 23-67.
Liu, Y., Chen, P., Zhou, M., Wang, T., Fang, S., Shang, X., & Fu, X. (2018). Geographic variation in the chemical composition and antioxidant properties of phenolic compounds from Cyclocarya paliurus (Batal) iljinskaja leaf. Molecules, 23(2440), 1-12. https://doi.org/10.3390/molecules23102440.
Mailandari, M. (2012). Uji aktivitas antioksidan ekstrak daun garcinia kydia roxb dengan metode DPPH dan identifikasi senyawa kimia fraksi yang aktif [Skripsi]. Universitas Indonesia.
Mazid, M., Khan, T. A., & Mohammad, F. (2011). Role of secondary metabolites in defense mechanisms of plants. Biology and Medicine, 3(2), 232-249.
Meria, M., da Silva, E. P., David, J., David, J. M., & David, J. P. (2012). Review of the genus Ipomea: traditional uses, chemistry and biological activities. Brazilian Journal of Pharmacognosy, 22(3), 682-713. https://doi.org/10.1590/S0102-695X2012005000025.
Mohanapriya, S., & Sridevi, J. (2020). Quantification of nutrient and phytoconstituent in fresh and dehydrated Piper betel Leaf. International Journal of Applied Research, 6, 01-04.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211-219.
Nusch, E. (1980). Comparison of different methods for chlorophyll and phaeopigment determination. Arch Hydrobiol Beih Ergebn Limnology, 14,14-36.
Parekh, K. K., Patel, A. M., Modi, A. J., & Chandrashekhar, H. R. (2012). Antioxidant and cytotoxic activities of few selected Ipomoea species. Pharmacologia, 3(9), 377-386.
Pereda-Miranda., E, Escalante-Sa´nchez., & Escobedo-Martı´nez, C. (2005). Characterization of lipophilic pentasaccharides from beach Morning Glory (Ipomoea pes-caprae). Journal of Natural Products, 68, 226-30. https://doi.org/10.1021/np0496340.
Pourmorad, F., Hosseinimerhr, S. J., & Shahabimajd, N. (2006) Antioxidant activity, phenol and flavonoid contents of some selected iranian medicinal plants. African Journal of Biotechnology, 5, 1142-1145.
Putri S. W. K., Nurhasana, D., Avidlyandi., Gustian, I., Sipriyadi, & Adfa, M. (2021). Aktivitas antibakteri ekstrak etanol daun tapak kuda (Ipomoea pes-caprae (L.) R. Br.) terhadap bakteri Staphylococcus epidermidis. BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains, 4(2), 355-362.
Ratnasooriya, W.D., Pathirana, R. N., Dissanayake, A. S., Samanmali, B. L. C., & Banu, R.S. (2017). Methanolic leaf extract of Ipomoea Pes-caprae possesses in vitro sun screen activity. Imperial Journal of Interdisciplinary Research, 3(2), 150-154.
Rezaeizadeh, A., Zuki, A. B. Z., Abdollahi, M., Goh, Y. M., Noordin, M. M., Hamid, M., & Azmi, T. I. (2011). Determination of antioxidant activity in methanolic and chloroformic extract of momordica charantia. African Journal of Biotechnology, 10(24), 4932-4940.
Robert, P, & Retna A. (2016). 5, 7, 4’ Trihydroxyisoflavone isolated from Ipomea pes-caprae roots by normal phase column chromatography. Bulletin Environment Pharmacology Life Science, 5(5), 27-33.
Sen, D. T., Tan, D. V., & La, H. T. (2013). Biological activity of methanolic extract derived from Ipomea pes-caprae (L.) collected in Xuan Thuy national park. Journal of Science Hnue, 58(9), 139-145.
Shanmugapriya, R., Ramanathan, T., Thirunavukkarasu, P., & Renugadevi, G. (2012). In vitro antifungal activity of Ipomoea pes-caprae (L.) R. Br. ethanolic flower extract. International Journal of Pharmaceutics, 3(1), 732-735.
Teramachi, F., Koyano, T., Kowithayakor, T., Hayashi, M., Komiyama, K., & Ishibashi, M. (2005). Collagenase inhibitory quinic acid ester from Ipomoea pes-caprae. Journal of Natural Products, 68, 794-796. https://doi.org/10.1021/np0500631.
Umamaheshwari, G., Ramanathan, T., & Shanmugapriya, R. (2012). Antioxidant and radical scavenging effect of Ipomea pescaprae Linn.R.BR. International Journal of PharmTech Research, 4(2), 848-851.
Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., & Telser. J. (2007) Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 39, 44–84. https://doi.org/10.1016/j.biocel.2006.07.001.
Venkataraman, N. D., Atlee, W. C., Prabhu, T. P., & Kannan. R. (2013). Anti-inflammatory potential of ethanolic extracts from aerial parts of Ipomoea pes-caprae (L.) RBr using cotton pellet induced granuloma model. Journal of Applied Pharmaceutical Science, 3(7), 61-63. https://doi.org/10.1016/j.jep.2005.07.029.
Vince, O. & Zoltan, M. (2011). Plant Physiology: Secondary metabolites in plant defences. http://www.tankonyvtar.hu/hu/tartalom/tamop425/0010_1A_Book_angol_01_nov enyelettan/ch03s05.html.
Vijayalaxmi, S. K, Jayalakshmi & K Sreeramulu. (2015). Polyphenols from different agricultural residues: extraction, identification and their antioxidant properties. Journal of Food Science and Technology, 52(5), 2761–2769.
Jacoeb, A. M., Suptijah, P., & Zahidah. (2013). Komposisi kimia, komponen bioaktif dan aktivitas antioksidan buah lindur (Bruguiera gymnorrhiza). Jurnal Pengolahan
Hasil Perikanan Indonesia, 16(1), 86-94. https://doi.org/10.17844/jphpi.v16i1.7772.
Widyawati, P. S. (2016). Determination of antioxidant capacity in pluchea indica less leaf extract and its fractions. International Journal of Pharmacy and Pharmaceutical Sciences, 8(9), 32-36. http://dx.doi.org/10.22159/ijpps.2016v8i9.11410.
Wink, M. (2008). Ecological roles of alkaloids. Wink, M. (Eds.) Modern Alkaloids, Structure, Isolation Synthesis and Biology. Wiley-VCH Verlag GmbH & Co. KgaA.
Woo, H., Kuleck, G. A., Hirsch, A. M., & Hawes, M. (2000). The role of flavonoids as signal molecules in plant development. Plant Biology, 11 (12), 2303-2316.
Authors
GazaliM., NufusH., SyafitriR., SarongM. A., & FadlyS. A. W. (2023). Evaluasi penangkapan radikal bebas DPPH ekstrak daun Ipomoea pes-caprae Linn asal Pantai Labuhan Haji, Aceh Selatan: The evaluation of DPPH free radical scavenging of Ipomoea pes-caprae leaf from the Beach of Labuhan Haji, South Aceh. Jurnal Pengolahan Hasil Perikanan Indonesia, 26(2), 340-349. https://doi.org/10.17844/jphpi.v26i2.43795
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