Aktivitas Antioksidan Kolagen dari Kulit Ikan Patin (Pangasius sp.) dengan Enzim Bromelin Kasar Kulit Nanas (Ananas comosus L.) Antioxidant Activity of Collagen from the Skin of Pangas Catfish (Pangasius sp) with Crude Bromelain Enzyme of Pineapple Peel
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Accepted
7 April 2022
Published
13 April 2022
Keywords:
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antioxidant, catfish, collagen, crude bromelyn enzyme
Abstract
Pangas catfish skin is one of fishery by-products containing certain compounds which have the potential to be used as an alternative source of collagen having antioxidant activity. The purpose of this study is to determine the antioxidant activity in collagen, so that it can be used as an antioxidant material. Collagen was made using crude bromelain enzyme from pineapple peel with an activity of 1.0 U/g in several stages, which were pretreatment, deproteinization, degrasing, and extraction, using factorial RAL with a combination of 3 hours, 3.5 hours and 4 hours and crude enzyme concentration of 0 ; 1.5% ; 2%. The results of the observations showed that the best solubility was obtained in the T3E2 treatment which was 98.96%. The best viscosity was 22.67 cP. The best acidity (pH) at T1.E2 was 6.73. The molecular weights α1, α2, β and ƴ were : 131.51 kDa, 110.48 kDa, 202.48 kDa and 243.93 kDa. The best antioxidant activity was in T1.E3 treatment, which was 20.45 ferrous sulfate/g.
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Shon j, Ji-Hyun E, Hwang SJ, Jong Bang E. 2011. Effect of processing conditions on functional properties of collagen powder from skate (Raja kenojei) skins. The
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Tabarestani SH, Maghsoudlou Y, Motamedzadega A, Mahoonak SAR, Rostamzad H. 2012. Study on some properties of acid-soluble colagens isoled from fish skin and bones of rainbow trout (Onchorhynchus mykiss). International Food Research Journal. 19(1):251-257.
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Wulandari. 2016. Karakteristik fisikokimia kolagen yang diisolasi dengan metode hidroekstraksi dan stabilisasi nanokolagen kulit ikan gabus (Channa Stiata) [tesis]. Bogor (ID): Institut Pertanian Bogor.
Wu RB, Wu CL, Liu D, Yang XH, Huang JF, Zhang J, Liao BQ. 2018. Antioxidant and anti-freezing peptides from salmon collagen hydrolysate prepared by bacterial extracellular protease. Food Chemistry. 248: 346-352.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radical and antioxidant in normal physiological function and human disease. The International Journal of Biochemistry & Cell Biology. 39(1): 44-84.
Ahmed R, Chun BS. 2018. Subcritical water hydrolysis for the production of bioactive peptides from tuna skin collagen. The Journal of Supercritical Fluids. 141(1): 1-35.
Astiana I. 2016. Efektifitas Asam Dan Enzim Papain Dalam Menghasilkan Kolagen Dari Kulit Ikan Ekor Kuning (Caesto cuning). [tesis]. Bogor (ID): Institut Pertanian Bogor.
Astiana I, Nurjanah, Nurhayati T. 2016. Karakterisasi kolagen larut asam dari kulit ikan ekor kuning (Caesto cuning). Jurnal Pengolahan Hasil Perikanan Indonesia. 19(1): 79-93.
[AOAC] Association of Official Analytical Chemist. 2005. Official Method of Analysis. 18 Ed. The Association of Official Analytical Chemist. Inc.
Baehaki A, Nopianti R, Anggraeni S. 2015. Antioxidant activity of skin and bone collagen hydrolyzed from striped catfish (Pangasius pangasius) with papain enzyme. Journal of Chemical and Pharmaceutical Research. 7(11): 131-135.
Baehaki A, Lestari SD, Desliani I. 2016. Collagen Hydrolysis from skin and bone of Pangasius catfish Prepared by Bromelain Enzyme and Antioxidant Activity of Hydrolysate. Der Pharma Chemica, 8 (4):155-158.
Bae I, Osatomi K, Yoshida A, Osako K, Yamaguchi A, Hara K. 2008. Chemical properties of acid-soluble collagens extracted from the skins of underutilized fishes. Food Chemistry. 108: 49-54.
[BSN] Badan Standardisasi Nasional. 2014. SNI 8076:2014. Standar Nasional Indonesia tentang kolagen kasar dari sisik ikan. Jakarta(ID):Badan Standardisasi Nasional.
Chi C, Wang B, Li Z-R, Luo H-Y, Ding G-F, Wu C-W. 2014. Characterization of acid-soluble collagen from the skin hammerhead shark (Sphyrna lewini). Journal of Food Biochemistry. 38: 236–247.
Devi HLNA, Suptijah P, Nurilmala M. 2017. Efektivitas alkali dan asam terhadap mutu kolagen dari kulit ikan patin. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 255–265.
Hadfi N.H. and Sarbon, N.M. 2019. Physicochemical properties of silver catfish (Pangasius sp.) skin collagen as influenced by acetic acid concentration. Food Research. 3(6) :783–790.
Haditono S, Idrus S. 2018. Proporsi dan kadar proksimat bagian tubuh ikan tuna ekor kuning (Thunnus albacares) dari Perairan Maluku. Majalah BIAM (Bahan Alam, Industri, Aneka Pangan, Minyak Atsiri) 14:51-57
Razali UHM, Qhairul A, Mamat H, Qhairul N. 2019. Properties of hydrolysed collagen from the skin of milkfish (Chanos chanos) as affected by different enzymatic treatments. International Journal of Research Science & Management. 6(2): 34-41.
Hastarini E, Fardiaz D, Irianto HE, Budijanto S. 2012. Karakteristik minyak ikan dari limbah pengolahan filet ikan patin siam (Pangasius hypopthalmus) dan patin jambal (Pangasius djambal). AGRITECH.32(4):403-410.
[KKP] Kementerian Kelautan dan Perikanan. 2018. Produktivitas Perikanan Indonesia Pusat Data Statistika dan Informasi Kementerian Kelautan dan Perikanan Tahun 2018. Jakarta (ID): Kementerian Kelautan dan Perikanan.
Murray BA, Fitzgerald RJ. 2007. Angiotensin-I converting enzyme inhibitory peptides derived from food proteins: Biochemistry,bioactivity and production. Current Pharmaceutical Design. 13(8): 773-791.
Ogawa M, Portier RJ, Moody MW, Bell J, Schexnayder MA, Losso JN. 2004. Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonis cromis) and sheepshead seabream (Archosargus probatocephalus). Food Chemistry. 88: 495-501.
Peng Y, Glattauer V, Werkmeister JA, Ramshaw JAM. 2004. Evaluation for collagen products for cosmetic application. Journal of Cosmetic Science. 55:327-342.
Pertiwi RM. 2016. Ekstraksi dan karakterisasi kolagen larut papain dari kulit ikan tuna sirip kuning [Skripsi]. Bogor (ID): Institut Pertanian Bogor.
Potaros T, Raksakhulthai N, Runglerdreangkrai j, Worawattanamateekul W. 2009. Characteristics of collagen from nile tilapie (Oreochromis niloticus) skin isolated by two different methods. Natural Science. 43(3):584-593.
Prastyo DT, Trilaksani W, Nurjanah. 2020. Aktivitas antioksidan hidrolisat kolagen kulit ikan nila (Oreochromis niloticus). Jurnal Pengolahan Hasil Perikanan Indonesia. 23(3):423-433.
Suptijah P, Indriani D, Wardoyo SE. 2018. Isolasi dan karakterisasi kolagen dari kulit ikan patin (Pangasius sp.). Sains Natural Universitas Nusa Bangsa. 8(1): 8-23.
Singh P, Benjakul S, Maqsood S, Kishimura H. 2011. Isolation and characterization of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry. 124: 97–105.
Sintusamran S, Benjakul S, Kishimura H. 2013. Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer). Food Chemistry 138:2435-2441.
Shon j, Ji-Hyun E, Hwang SJ, Jong Bang E. 2011. Effect of processing conditions on functional properties of collagen powder from skate (Raja kenojei) skins. The
Journal of Food Science Biotechnology. 20 (1):99-106.
Tabarestani SH, Maghsoudlou Y, Motamedzadega A, Mahoonak SAR, Rostamzad H. 2012. Study on some properties of acid-soluble colagens isoled from fish skin and bones of rainbow trout (Onchorhynchus mykiss). International Food Research Journal. 19(1):251-257.
Walters BD, Stagemann JP. 2014. Review: Stategies for directing the structure and function of three-dimensional collagen biomaterials across length scales. Acta Biomaterialia. 10(4):1488-1501.
Wulandari. 2016. Karakteristik fisikokimia kolagen yang diisolasi dengan metode hidroekstraksi dan stabilisasi nanokolagen kulit ikan gabus (Channa Stiata) [tesis]. Bogor (ID): Institut Pertanian Bogor.
Wu RB, Wu CL, Liu D, Yang XH, Huang JF, Zhang J, Liao BQ. 2018. Antioxidant and anti-freezing peptides from salmon collagen hydrolysate prepared by bacterial extracellular protease. Food Chemistry. 248: 346-352.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Free radical and antioxidant in normal physiological function and human disease. The International Journal of Biochemistry & Cell Biology. 39(1): 44-84.
Authors
YantiF., DharmayantiN., & SuryantiS. (2022). Aktivitas Antioksidan Kolagen dari Kulit Ikan Patin (Pangasius sp.) dengan Enzim Bromelin Kasar Kulit Nanas (Ananas comosus L.): Antioxidant Activity of Collagen from the Skin of Pangas Catfish (Pangasius sp) with Crude Bromelain Enzyme of Pineapple Peel. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(1), 88-96. https://doi.org/10.17844/jphpi.v25i1.36731
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