Potensi Kolonisasi Mikoriza Arbuskular dan Cendawan Endofit dan Kemampuannya dalam Pengendalian Layu Fusarium pada Bawang Merah

  • Mei Lita Fitriani Institut Pertanian Bogor
  • Suryo Wiyono IPB University
  • Meity Suradji Sinaga IPB University
Keywords: antagonism mechanism, biocontrol agent, biocontrol combination

Abstract

Colonization Potential of Arbuscular Mycorrhiza and Endophytic Fungi and Its Effectiveness in Control Fusarium Wilt on Shallot


Fusarium wilt is an important disease on shallot (Allium cepa var. Aggregatum) due to Fusarium oxysporum f.sp. cepae. The research aimed to study colonization of arbuscular mycorrhiza and endophytic fungi, and their biocontrol performance against fusarium wilt disease of shallot. The experiment was performed using a completely randomized design with 8 treatments and 5 replications. The effectiveness of biocontrol agents on the progress of fusarium wilt was observed from the percentage of agent biocontrol colonization¸ disease latent periode¸ disease incidence¸ rate of infection¸ and area under disease progress curve. The biocontrol agent tested successfully symbiotic in the root tissue of shallot. The single application of arbuscular mycorrhizal showed the best influence on plant growth and suppressing the progress of fusarium wilt in shallots with the longest disease latent period at 21 days and decreasing disease incidence up to 40%.

Downloads

Download data is not yet available.

References

Agrios GN. 2005. Plant Pathology. Ed ke-5. California (US): Elsevier Academic Pr.

Al-Askar AA, Rashad YM. 2010. Arbuscular mycorrhizal fungi: Biocontrol agent against common bean Fusarium root rot disease. Plant Pathol J. 9(1):31–38. DOI: https://doi.org/10.3923/ppj.2010.31.38.

Bakhtiar Y, Yahya S, Sumaryono W, Sinaga MS, Budi SW, Tajuddin T. 2010. Isolation and identification of mycorrhizosphere bacteria and their antagonistic effect towards Ganoderma boninense in vitro. Microbiol Indones. 4:96–102. DOI: https://doi.org/10.5454/mi.4.2.9.

Bakhtiar Y. 2011. Peran fungi mikoriza arbuskular dan bakteri endosimbiotik mikoriza dalam meningkatkan daya adaptasi bibit kelapa sawit (Elaeis guineensis Jacq) terhadap cekaman biotik Ganoderma boninense Pat [disertasi]. Bogor (ID): Institut Pertanian Bogor.

Brundrett M, Bougher N, Dell B, Grove T, Malajczuk N. 1996. Working With Mycorrhizas In Forestry and Agriculture (No. 435-2016-33680). Canberra (AU): Australian Centre for International Agricultural Research.

Cruz AF, Ishii T. 2012. Arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soilborne plant pathogens. Biol Open. 1:52–57. DOI: https://doi.org/10.1242/bio.2011014.

Fadhilah S, Wiyono S, Surahman. 2014. Pengembangan Teknik Deteksi Fusarium Patogen Pada Umbi Benih Bawang Merah (Allium ascalonicum) di Laboratorium. J Horti. 24(2):171–178. DOI: https://doi.org/10.21082/jhort.v24n2.2014.p171-178.

Fesel PH, Zuccaro A. 2016. Dissecting endophytic lifestyle along the parasitism/mutualism continuum in Arabidopsis. Curr Opin Microbiol. 32:103–112. DOI: https://doi.org/10.1016/j.mib.2016.05.008.

Khairani HG, Sinaga MS, Mutaqin KH. 2017. Mekanisme pengendalian penyakit busuk batang jeruk oleh khamir, kitosan, cendawan mikoriza arbuskular, dan bakteri simbiotiknya. J Fitopatol Indones.13(1):17–25. DOI: https://doi.org/10.14692/jfi.13.1.

Leslie JF, Summerell BA, Bullock S. 2006. The Fusarium laboratory manual. Manhattan (US): Blackwell Publishing. DOI: https://doi.org/10.1002/9780470278376.

Muller JL. 2015. Plants and endophytes: equal partners in secondary metabolite production?. Biotechnol Lett. DOI: https://doi.org/10.1007/s10529-015-1814-4.

Ozgönen H, Akgul DS, Erkilic A. 2010. The effects of arbuscular mycorrhizal fungi on yield and stem rot caused by Sclerotium rolfsii Sacc. in peanut. African J Agric Res. 5(2):128–132.

Priyadharsini P, Pandey RR, Muthukumar T. 2012. Arbuscular mycorrhizal and dark septate fungal associations in shallot (Allium cepa L. var. aggregatum) under conventional agriculture. Acta Bot Croat. 71(1):159–175. DOI: https://doi.org/10.2478/v10184-011-0058-1.

Talapatra K, Das AR, Saha AK, Das P. 2017. In vitro antagonistic activity of a root endophytic fungus towards plant pathogenic fungi. J App Biol Biotech. 5(2):68–71. DOI: https://doi.org/10.7324/JABB.2017.50210.

Tondok ET. 2001. Twisting disease caused by Fusarium oxysporum on shallot (Allium cepa L. var. agregatum G. Don.) in Indonesia. [thesis]. Jerman (DE): Institute of Plant Protection, Faculty of Agriculture, George-August University Geottingen, Germany.

Wiyatiningsih S, Arif W, Endang TP. 2009. Keparahan penyakit moler pada enam kultivar bawang merah karena infeksi Fusarium oxysporum f.sp. cepae di tiga daerah sentra produksi. Seminar Nasional Akselerasi Pengembangan Teknologi Pertanian dalam Mendukung Revitalisasi Pertanian. 2009 Des 2. Surabaya (ID). Fak. Pertanian dan LPPM UPN Veteran Jawa Timur.

Xu XM, Jeffries P, Pautasso M, Jegger MJ. 2011. Combined use of biocontrol agent to manage plant disease in theory and practice. Phytopathology 1001(9):1024–1031. DOI: https://doi.org/10.1094/PHYTO-08-10-0216.

Zuccaro A, Lahrmann U, Langen G. 2014. Broad compatibility in fungal root symbioses. Curr Opin Plant Biol. 20:135–145. DOI: https://doi.org/10.1016/j.pbi.2014.05.013.

Zulaika. 2014. Pemanfaatan cendawan endofit dalam pengendalian busuk umbi (Fusarium oxysporum) pada bawang merah (Allium cepa var. aggregatum) [skripsi]. Bogor (ID):Institut Pertanian Bogor.

Published
2020-02-09
How to Cite
FitrianiM. L., WiyonoS., & SinagaM. S. (2020). Potensi Kolonisasi Mikoriza Arbuskular dan Cendawan Endofit dan Kemampuannya dalam Pengendalian Layu Fusarium pada Bawang Merah. Jurnal Fitopatologi Indonesia, 15(6), 228-238. https://doi.org/10.14692/jfi.15.6.228-238
Section
Articles