Begomovirus yang Berasosiasi dengan Gejala Mosaik Kuning pada Tanaman Terung di Bengkulu
Begomovirus Associated with Yellow Mosaic Symptom on Eggplant Plant in Bengkulu
Abstract
Eggplant (Solanum melongena) is a horticultural crop that has high economic value and is widely cultivated. One of the obstacles to eggplant cultivation is virus infection that causes diseases. In several eggplant fields in Bengkulu, systemic symptoms of yellow mosaic were found which were similar to Begomovirus infection in chilies. Research was conducted to detect and identify Begomovirus as the causal agent of yellow mosaic symptoms on eggplant plants in Bengkulu and its association with betasatellites. Samples were taken by purposive sampling from eggplant fields in Bengkulu City, Central Bengkulu Regency, North Bengkulu Regency, Kepahiang Regency, and Rejang Lebong Regency. Virus detection was carried out by PCR method using Begomovirus universal primers and betasatellite specific primers followed by confirmation of virus identity by DNA sequencing. PCR using universal Begomovirus and betasatellite primers was successfully amplified DNA fragments measuring ±912 bp and ±1300 bp, respectively from leaf samples with symptoms of yellow mosaic, leaf malformation and thickening of leaf veins. Based on DNA sequencing, samples from North Bengkulu and Kepahiang showed the highest homology 99% with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) from Indonesia, Malaysia and Thailand. These findings are the first reports of TYLCKaV infection and its association with Begomovirus betasatellites on eggplant in Bengkulu.
Downloads
References
Ali F, Aprilia RL. 2018. Serangan virus kuning terung pada induksi ekstrak daun Clerodendrum japonicum dan Mirabilis jalapa. Agrovigor: Jurnal Agroekoteknologi, 11(2):101–105. DOI: https://doi.org/10.21107/agrovigor.
Al-ani RA, Adhab MA, Ismail KAH. 2011. Eggplant blister mottled virus (EBMV) a possible new Potyvirus characterized from Iraq. Journal of General and Molecular Virology. 3(3):49–52.
Dombrovsky A, Pearlsman M, Iachman O, Antignus Y. 2009. Characterization of a new strain of Eggplant mottled crinckle virus (EMC) infecting eggplants in Israel. Phytoparasitica. 37(5):477–483. DOI: https://doi.org/10.1007/s12600-009-0058-z.
Doyle JJ, Doyle JJ. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19(1):11–15.
Gaswanto R, Syukur M, Purwoko BS, Hidayat SH. 2016. Induced mutation by gamma rays irradiation to increase chilli resistance to Begomovirus. Agrivita 38:24–32. DOI: https://doi.org/10.17503/Agrivita.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S. 2019. Molecular characterization of betasatellite associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java. Jurnal Perlindungan Tanaman Indonesia 23(2):292–298. DOI: https://doi.org/10.22146/jpti.46579.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S. 2021. A recombinant DNA-satellite associated with Pepper yellow leaf curl Indonesia virus in highland area. Indonesian Journal of Biotechnology. 26(2):82–90. DOI: https://doi.org/10.22146/ijbiotech.64817.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S, Widyasari YA. 2020. First report of naturally occurring recombinant non-coding DNA satellite associated with Tomato yellow leaf curl Kanchanaburi virus on eggplant in Indonesia. Biodiversitas Journal of Biological Diversity. 21(1):129–136. DOI: https://doi.org/10.13057/biodiv/d210117.
Kintasari T, Septariani DWN, Sulandari S, Hidayat SH. 2013. Tomato yellow leaf curl Kanchanaburi virus penyebab penyakit mosaik kuning pada tanaman terung di Jawa. Jurnal Fitopatologi Indonesia. 9(4):127–131. DOI: https://doi.org/10.14692/jfi.9.4.127.
Kesumawati, E, Okabe S, Khalil M, Alfan G, Bahagia P, Pohan N, Zakaria S, Koeda S. 2020. Molekular characterization of Begomovirus associated with yellow leaf curl disease of Solanaceae and Cucurbitaceae Crop in Northern Sumatra, Indonesia. The Horticulture Journal. 89:410–416. DOI: https://doi.org/10.2503/hortj.UTD-175.
Kon T, Hidayat SH, Hase S, Takahashi H, Ikegami M. 2006. The natural occurrence of two distinct begomoviruses associated with DNA and a recombinant DNA in a tomato plant from Indonesia. Phytopathology. 96(5):517–525. DOI: https://doi.org/10.1094/PHYTO-96-0517.
Kon T, Kuwabara K, Hidayat SH, Ikegami M. 2007. A Begomovirus associated with Ageratum yellow vein disease in Indonesia: evidence for natural recombination between Tomato leaf curl Java virus and Ageratum yellow vein virus-[Java]. Archives of virology. 152:1147–1157. DOI: https://doi.org/10.1007/s00705-006-0928-3.
Listihani L, Damayanti TA, Hidayat SH, Wiyono S. 2019. Molecular characterization of Begomovirus on cucumber in Java. Jurnal Perlindungan Tanaman Indonesia. 23(2):197–204. DOI: https://doi.org/10.22146/jpti.41402.
Li R, Salih S, Hurtt S. 2004. Detection of geminiviruses in sweetpotato by polymerase chain reaction. Plant disease. 88(12):1347–1351. DOI: https://doi.org/10.1094/PDIS.2004.88.12.1347.
Morales FJ, Anderson PK. 2001. The emergence and dissemination of whitefly transmitted geminiviruses in Latin America. Journal of Virology. 146(3):415–441. DOI: https://doi.org/10.1007/s007050170153.
Rojas MR, Macedo MA, Maliano MR, Soto-Aguilar M, Souza JO, Briddon RW, Kenyon L, Rivera Bustamante RF, Zerbini FM, Adkins S, Legg JP. 2018. World management of geminiviruses. Annual review of phytopathology. 25(56):637–677. DOI: https://doi.org/10.1146/annurev-phyto-080615-100327.
Rahim YF, Damayanti TA, Ghulamahdi M. 2015. Deteksi virus yang menginfeksi kedelai di Jawa. Jurnal Fitopatologi Indonesia. 11(2):59–67. DOI: https://doi.org/10.14692/jfi.11.2.59 .
Septariani, DN, Hidayat SH, Nurhayati E. 2014. Identifikasi penyebab penyakit daun keriting kuning pada tanaman mentimun. Jurnal Hama dan Penyakit Tumbuhan Tropika. 14(1):80–86. DOI: https://doi.org/10.23960/j.hptt.11480-86.
Singh AK, Kushwaha N, Chakraborty S. 2016. Synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli. Applied Microbiology and Biotechnology. 100:4035–4049. DOI: https://doi.org/10.1007/s00253-015-7279-5.
Subiastuti AS, Hartono S, Daryono BS. 2019. Detection and identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas Journal of Biological Diversity. 20(3):738–744. DOI: https://doi.org/10.13057/biodiv/d200318.
Sutrawati M, Parwito, Priyatiningsih, Zarkani A, Sipriyadi, Sariasih Y, Ganefianti DW. 2021. First Report of Begomovirus Infection on Papaya in Bengkulu Indonesia. Jurnal Hama Penyakit Tanaman Tropika. 21(1):49–55. DOI: https://doi.org/10.23960/jhptt.12149-55.
Sutrawati M, Hidayat SH, Suastika D, Sukarno BPW, Nurmansyah A. 2020. Penyakit mosaik kuning pada kedelai. Jurnal Fitopatologi Indonesia. 16(1):30–36. DOI: https://doi.org/10.14692/jfi.16.1.30-36.
Vivaldy LA, Ratulangi MM, Manengkey GSJ. 2017. Insidensi penyakit virus pada tanaman cabai (Capsicum anuum) di Desa Kakaskasen II Kecamatan Tomohon Utara Kota Tomohon. Cocos e-Jurnal Sam Ratulangi University. 1(6):1–9.
Zerbini FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J, Rivera-Bustamante R, Roumagnac P, Varsani A, ICTV Report Consortium. 2017. ICTV virus taxonomy profile: Geminiviridae. Journal of general virology. 98(2):131–133. DOI: https://doi.org/10.1099/jgv.0.000738.
Zhou X. 2013. Advances in understanding Begomovirus satellites. Annual Review of Phytopathology. 51:357–381. DOI: https://doi.org/10.1146/annurev-phyto-082712-102234.
Copyright (c) 2024 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
