Kejadian Pertama Infeksi Zucchini yellow mosaic virus pada Tanaman Mentimun di Padang, Sumatera Barat
Abstract
The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra
Cucumber Local Padang variety is considered as a superior vegetable crop by urban farmers due to its early harvest time. Yellow spots and chlorosis symptoms was found on cucumber plants during field surveys conducted recently. These symptoms are similar to Papaya ring spot virus (PRSV) infection on cucumbers in Java, and common symptoms of Zucchini yellow mosaic virus (ZYMV) infection on cucurbits. Detection of the causal virus was carried out by the reverse transcription polymerase chain reaction (RT-PCR) using PRSV specific primer and universal primers of Potyvirus, DNA sequencing and analysis. DNA target was not amplified by RT-PCR using PRSV specific primer, but DNA fragment of ±320 bp was successfully amplified using Potyvirus universal primer. The nucleotide sequences analysis showed highest similarity of 93.7% and 93.5% to the ZYMV isolates from Singapore and India, respectively. This is the first report on the occurrence of ZYMV infection on cucumber plants in Padang, West Sumatra.
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References
Ali A, Mohammad O, Khattab A. 2012. Distribution of viruses infecting cucurbit crops and isolation of potential new virus-like sequences from weeds in Oklahoma. Plant Disease 96:243–248. DOI: https://doi.org/10.1094/PDIS-05-11-0419.
Damayanti TA, Nurjanah T, Listihani L, Hidayat SH, Wiyono S. 2022. Characterization of a variant isolate of Zucchini yellow mosaic virus infecting green kabocha (Cucurbita maxima L.) in Bogor, Indonesia. Archives of Phytopathology and Plant Protection 55(1):121–128. DOI: https://doi.org/10.1080/03235408.2021.2003604.
Farida N, Damayanti TA, Efendi D, Hidayat SH. 2022. Insidensi dan identifikasi molekuler Papaya ring spot virus pada pepaya di Jawa. Jurnal Fitopatologi Indonesia . 18(1):43–51. DOI: https://doi.org/10.14692/jfi.18.1.43-51.
Haerunisa R, Suastika G, Damayanti TA. 2014. Identifikasi Begomovirus yang berasosiasi dengan penyakit kuning pada mentimun di Jawa Barat dan Bali. Jurnal Hortikultura Indonesia 7(1):9–20. DOI: https://doi.org/10.29244/jhi.7.1.9-20.
Harmiyati T, Hidayat SH, Adnan AM. 2015. Deteksi dan respons lima varietas pepaya terhadap tiga isolat Papaya ring spot virus (PRSV). Jurnal AgroBiogen. 11(3):87–94. DOI: https://doi.org/10.21082/jbio.v11n3.2015.p87-94.
Hseu SH, Huang CH, Chang CH, Yang WZ, Chang YM, Hsio CH. 1987. The occurance of five viruses in six cucurbits in Taiwan. Plant Protection Bulletin. 29(3):233–244.
Laili NU, Damayanti TA. 2019. Identifikasi virus pada tanaman mentimun di Jawa Barat. Agrovigor. 12(1):8–15. DOI: https://doi.org/10.33462/jotaf.707645.
Listihani, Damayanti TA, Hidayat SH, Wiyono S. 2018a. Karakterisasi molekuler Papaya ringspot virus tipe P pada tanaman mentimun di Jawa. Jurnal Fitopatologi Indonesia. 13(3):75–82. DOI: https://doi.org/10.14692/jfi.14.3.75.
Listihani, Damayanti TA, Hidayat SH, Wiyono S. 2018b. Zucchini yellow mosaic potyvirus (ZYMV) on Cucumber (Cucumis sativus L.) in Java, Indonesia. Proceedings of SEAMEO BIOTROP Third International Conference on Tropical Biology “Conservation, Enhancement: and Sustainable Use of Indigenous Tropical Flora and Fauna”. p.116–119.
Listihani, Damayanti TA, Hidayat SH, Wiyono S. 2019. Molecular characterization of Begomovirus on cucumber in Java. Jurnal Perlindungan Tanaman Indonesia 23(2): 197–204. DOI: https://doi.org/10.22146/jpti.41402.
Marie-Jeanne V, Ioos R, Peyre J, Alliot B, Signoret P. 2000. Differentiation of poaceae Potyviruses by reverse transcription polymerase chain reaction and restriction analysis. Journal of Phytopathology. 148:141–151. DOI: https://doi.org/10.1046/j.1439-0434.2000.00473.x.
Mohammed H, Manglli A, Zicca S, El Hussein A, Mohamed M, Tomassoli L. 2012. First report of Papaya ring spot virus in pumpkin in Sudan. New Disease Report. 26:26. DOI: https://doi.org/10.5197/j.2044-0588.2012.026.0266.
Pandawani NP, Widnyana IK. 2021. Identıfıcatıon of virus causes of mosaıc dıseases in zucchını plants in the Bali island of Indonesıa. Tekirdağ Ziraat Fakültesi Dergisi, 18 (3):411–418. DOI: https://doi.org/10.33462/jotaf.707645.
Septariani DN, Hidayat SH, Nurhayati E. 2014. Identifikasi penyebab daun keriting kuning pada mentimun. Jurnal HPT Tropika 14(1):80–86. DOI: https://doi.org/10.23960/j.hptt.11480-86.
Shtayeh MSA, Jamous RM, Husein EY, Alkhader MY. 2010. First report of squash leaf curl in squash (Cucurbita pepo), melon (Cucumis melo), and cucumber (Cucumber sativus) in the Northern West Bank of the Palestinian Authority. Plant Disease 94(5):640. DOI: https://doi.org/10.1094/PDIS-94-5-0640B.
Spadotti DMA, Wassano DT, Rezende JAM, Camargo LEA, Nagata AKI. 2015. Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus. Scientia Agricola 72(2):187–191. DOI: https://doi.org/10.1590/0103-9016-2014-0197.
Sutarya R. 2005. Deteksi penyakit yang disebabkan oleh virus pada tanaman mentimun. Laporan intern ATA-395.
Wiratama IDMP, Wirya GNAS, Andyani NNP, Nyana IDN, Suastika G. 2015. Laporan pertama infeksi Begomovirus pada mentimun di Bali. Jurnal Fitopatologi Indonesia 11(5):175–178. DOI: https://doi.org/10.14692/jfi.11.5.175.
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