Identifikasi Molekuler Satelit DNA yang Berasosiasi dengan Mungbean yellow mosaic India virus pada Kacang Panjang di Yogyakarta
Abstract
Molecular Identification of Mungbean yellow mosaic India virus and Its Betasatellite Associated with Yellow Mosaic on Yardlong Bean in Sleman, Yogyakarta
Yellow mosaic and leaf curl symptoms was observed in yard long baen cultivation in Sleman, Yogyakarta. Begomovirus is known as one of the causal agent of this disease. Research was conducted to identify species of Begomovirus and the associated DNA satellites from yellow mosaic disease of yard long bean. Total DNA was extracted from symptomatic leaves. DNA amplification was carried out through polymerase chain reaction using universal primer for Begomovirus and specific primer for Betasatellite. DNA fragments of 1500 bp and 1300 bp was successfully amplified which indicated the presence of Begomovirus and DNA satellite in symptomatic leaves, respectively. Nucleotide sequence analysis showed that Begomovirus infecting yardlong bean has close relationship to Mungbean yellow mosaic India virus isolate from Indonesia with 99% homology. DNA satellite associated with MYMIV showed characteristic of Betasatellites, i.e. stem-loop structure in satellite conserve regions (SCR) TAATATTAC sequence in the loop region, adenine rich region (54.96%), and a non-coding ORF (open reading frame). SimPlot analysis suggests that the non-coding satellite associated with MYMIV was a recombinant satellite between betasatellite and DNA-B PepYLCIV (Pepper yellow leaf curl Indonesia virus). This study is the first report of the non-coding DNA satellite associated with MYMIV in Indonesia.
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References
Briddon RW, Markham PG. 1994. Universal primers for the PCR amplification of dicot-infecting geminiviruses. Mol Biotechnol. 1:202–205. DOI: https://doi.org/10.1007/BF02921559.
Damayanti TA, Alabi OJ, Naidu RA, Rauf A. 2009. Severe outbreak of a yellow mosaic disease on the yard long bean in Bogor, West Java. Hayati J Biosci.16(2):78–82. DOI: https://doi.org/10.4308/hjb.16.2.78.
Gilbertson RL, Batuman O, Webster CG, Adkins S. 2015. Role of the Insect Supervectors Bemisia tabaci and Frankliniella occidentalis in the Emergence and Global Spread of Plant Viruses. Annu Rev Virol. 2(1):67–93. DOI:https://doi.org/10.1146/annurev-virology-031413-085410.
Huang C, Xie Y, Zhao L, Ren H, Li Z. 2013. A naturally occurring defective DNA satellite associated with a monopartite Begomovirus: evidence for recombination between alphasatellite and betasatellite. Viruses 5: 2116–2128. DOI: https://doi.org/10.3390/v5092116.
Ilyas M, Qazi J, Mansoor S, Briddon RW. 2010. Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan. J Gen Virol. 91(8):2091–2101. DOI: https://doi.org/10.1099/vir.0.020404-0.
Jamsari J, Pedri J. 2013. Complete nucleotide sequence of DNA A-like genome and DNA-β of monopartite Pepper yellow leaf curl virus, a dominant Begomovirus infecting Capsicum annuum in West Sumatera Indonesia. Asian J Plant Pathol. 7 (1): 1–14. DOI: https://doi.org/10.3923/ajppaj.2013.1.14.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S, Widyasari YA. 2020. First report of naturally occurring recombinant non-coding DNA satellite associated with Tomato yellow leaf curl Kanchanaburi virus on eggplant in Indonesia. Biodiversitas. 21(1):129–136. DOI: https://doi.org/10.13057/biodiv/d210117.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S. 2019. Molecular characterization of betasatellite associated with begomovirus on Ageratum conyzoides in Magelang, Central Java. JPTI. 23(2):292–298. DOI: https://doi.org/10.22146/jpti.46579.
Kandito A, Hartono S, Sulandari S, Somowiyarjo S. 2021. A recombinant DNA-satellite associated with Pepper yellow leaf curl Indonesia virus in highland area. Indonesian. J Biotech. 26(2):82–90. DOI: https://doi.org/10.22146/ijbiotech.64817.
Kon T, Hidayat SH, Hase S, Takahashi H, Ikegami M. 2006. The natural occurrence of two distinct begomoviruses associated with DNA and a recombinant DNA in a tomato plant from Indonesia. Phytopathology 96(5):517–525. DOI: https://doi.org/10.1094/PHYTO-96-0517.
Kon T, Kuwabara K, Hidayat SH, Ikegami M. 2007. A Begomovirus associated with ageratum yellow vein disease in Indonesia: evidence for natural recombination between Tomato leaf curl Java virus and Ageratum yellow vein virus-[Java]. Arch Virol. 152: 1147–1157. DOI: https://doi.org/10.1007/s00705-006-0928-3.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33(7):1870–1874. DOI: https://doi.org/10.1093/molbev/msw054.
Marwal A, Prajapat R, Gaur RK. 2016. First report of recombination analysis of betasatellite and alphasatellite sequence isolated from an ornamental plant marigold in India: an in silico approach. Int J Virol. 12(1):10–17. DOI: https://doi.org/10.3923/ijv.2016.10.17.
Nurulita S, Hidayat SH, Mutaqin KH, Thomas J. 2015. Molecular characterization of Begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia. Biotropia. 1(22):53–60. DOI: https://doi.org/10.11598/btb.2015.22.1.401.
Purwoko RR, Hartono S, Suputa, Lukman R, Wahyudin D. 2015. Emerging Pepper yellow leaf curl virus and Mungbean yellow mosaic virus of single Bemisia tabaci in Java, Indonesia. Di dalam: The 11th International Student Conference; 2015 Desember 5–6; Ibaraki (JP): Ibaraki University.
Ray SC. 2003. SimPlot for Windows (version 3.5.1.). Distributed by the author Baltimore, MD, USA. https://sray.med.som.jhmi.edu/SCRoftware/simplot/. [diakses 27 April 2021].
Rojas MR, Gilbertson RL, Russell DR, Maxwell DP. 1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Dis 77:340–347. DOI: 10.1094/PD-77-0340.
Rouhibakhsh A, Malathi VG. 2005. Severe leaf curl disease of cowpea – a new disease of cowpea in Northern India caused by Mungbean yellow mosaic India virus and a satellite DNA β. Plant Pathol. 54:259. DOI: https://doi.org/10.1111/j.1365-3059.2005.01139.x.
Satya VK, Malathi VG, Velazhahan R, Rabindran R, Jayamani P, Alice D. 2013. Characterization of betasatellite associated with the yellow mosaic disease of grain legumes in Southern India. Acta Virol. 57:405–414. DOI: https://doi.org/10.4149/av_2013_04_405.
Shahid MS, Briddon RW, Al-Sadi AM. 2017. Identification of Mungbean yellow mosaic Indian virus associated with Tomato leaf curl betasatellite infecting Phaseolus vulgaris in Oman. J Phytopathol. 165(3):204–211. DOI: https://doi.org/10.1111/jph.12551.
Sidik EA, Hartono S, Sulandari S, Lukman R, Affifudin A, Wahyudin D, Santoso HS. 2017. Molecular evidence for mixed infection of four begomovirus in common bean and yard long bean showing severe yellow symptoms in East Java, Indonesia. Di dalam: Isnantyo A, Nuringtyas TR, editor. Proceeding of The 1st International Conference on Tropical Agriculture. Switzerland (CH): Springer. hlm. 73–84. DOI: https://doi.org/10.1007/978-3-319-60363-6_7.
Subiastuti AS, Hartono S, Daryono BS. 2019. Detection and identification of Begomovirus infecting Cucurbitaceae and Solanaceae in Yogyakarta, Indonesia. Biodiversitas. 20(3):738–744. DOI: https://doi.org/10.13057/biodiv/d200318.
Sutrawati M, Hidayat SH, Suastika G, Sukarno BPW, Nurmansyah A. 2020. Penyakit mosaik kuning pada kedelai. J Fitopatol Indones. 16(1):30–36. DOI: https://doi.org/10.14692/jfi.16.1.30-36.
Tao X, Zhou X. 2008. Pathogenicity of a naturally occurring recombinant DNA satellite associated with Tomato yellow leaf curl China virus. J Gen Virol. 89: 306–311. DOI: https://doi.org/10.1099/vir.0.83388-0.
Wilisiani F. 2019a. Deteksi Begomovirus pada tanaman cabai di Magelang Indonesia. AGROISTA: J Agrotek. 3(01): 1–10.
Wilisiani F, Mashiko T, Wang WQ, Suzuki T, Hartono S, Neriya Y, Nishigawa H, Natsuaki T. 2019b. New recombinant of Tomato leaf curl New Delhi virus infecting melon in Indonesia. J Gen Plant Pathol. 85(4):306–310. DOI: https://doi.org/10.1007/s10327-019-00849-7.
Zhou X. 2013. Advances in understanding begomovirus satellite. Ann Rev Phytopathol. 51(1):357–381. DOI: https://doi.org/10.1146/annurev-phyto-082712-102234.
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