Pengendalian Hayati Penyakit Busuk Bulir Bakteri yang Disebabkan oleh Burkholderia glumae Menggunakan Aktinomiset

  • Nurmujahidin
  • Giyanto Giyanto
  • Dadang Departemen Proteksi Tanaman IPB University
Keywords: bioactive compound, biological agents, rhizosphere, severity of disease, rice


Biological Control of Bacterial Grain Rot Disease Caused by Burkholderia glumae Using Actinomycetes

Bacterial grain rot disease is an important disease in rice plants caused by the bacterium Burkholderia glumae and causes yield loss up to 70%. In general, it is recommended to control this disease by seed treatment using various biological agents, including actinomycetes which produce antibacterial bioactive compounds. The aim of this study was to obtain actinomycetes strains that have the potential to control bacterial grain rot caused by B. glumae. The stages of the research included isolating and screening actinomycetes as potential biological agents against B. glumae, testing the phytotoxicity of actinomycetes on rice seeds, and testing the effectiveness of actinomycetes in controlling B. glumae on rice in the nursery phase. There were 40 actinomycetes isolated from rice plants and the rhizosphere and 17 of them had no potential as pathogens for plants or mammals. The suppression potency of the actinomycetes based on antibiosis test yielded 7 actinomycetes strains that were able to suppress the development of B. glumae and 5 strains had an inhibition zone of more than 2 mm, were not phytotoxic to rice plants and had the ability to promote plant growth. This actinomycete strain affects the growth of B. glumae and also suppresses the degree of its infection. Actinomycetes ST1, ST27, and BT23 strains were effective in suppressing bacterial grain rot in the nursery phase.


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Aldesuquy HS, Mansour FA, Abo-Hamed SA. 1998. Effect of the culture filtrates of Streptomyces on growth and productivity of wheat plants. Folia Microbiologica. 43:465–470. DOI:

Baharuddin, Harniati R, Faisal F, Yani A, Suparmi, Hamid H, Kuswinanti T, Jahuddin R. 2017. Keberadaan penyakit busuk bulir (Burkholderia glumae) pada tanaman padi di Sulawesi Selatan. Di dalam: Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia “Kemunculan Penyakit Baru dan Impor Benih; 2017 Jan 10; Bogor (ID): Departemen Proteksi Tanaman, IPB University. hlm 19-26.

Beutin L. 1991. The different haemolysins of Eschericia coli. Medical Microbiology Immunology. 180(4):167–182. DOI:

Cao L, Qiu Z, You J, Tan H, Zhou S. 2004. Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots. Letters in Applied Microbiology. 39(5):425–430. DOI:

Crawford DL, Lynch JM, Whipps JM, Ousley MA. 1993. Isolation and characterization of actinomycete antagonist of a fungal root pathogen. Applied and Enviromental Microbiology. 59(11):3899–3905. DOI:

De Fretes CE, Sembiring L, Purwestri YA. 2013. Characterization of Streptomyces spp. producing indole-3-acetic acid as biostimulant agent. Indonesian Journal of Biotechnology. 18(2):83–89. DOI:

Flórez-Zapata NMVF, Uribe-Vélez D. 2011. Determination of the infection of Burkholderia glumae in commercial colombian cice varieties. Revista Facultad Nacional de Agronomia Medellín. 64(2):6093–6104.

Hikichi Y. Mode of action of oxolinic acid against bacterial seedling rot of rice caused by Pseudomonas glumae 1. Relationship between population dynamics of P. glumae on seedling of rice and disease severity of bacterial seedling rot of rice. Annals of the Phytopathological Society of Japan. 59(4):441–446. DOI:

Hoa PTP, Quang ND, Sakiyama Y, Hop DV, Hang DT, Ha TH, Van NT, Quy NTK, Da NTA. 2012. Screening for actinomyces isolated from soil with the ability to inhibit Xanthomonas oryzae pv. oryzae causing rice blight disease in Vietnam. African Journal of Biotechnology. 11(80):14586–14594. DOI:

Husen E. 2003. Screening of soil bacteria for plant growth promotion activities in vitro. Indonesian Journal of Agricultural Science. 4(1):27–31. DOI:

Joko T. 2017. Burkholderia glumae sebagai emerging pathogen: status, potensi kerusakan, dan strategi pengendalian. Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia “Kemunculan Penyakit Baru dan Impor Benih; 2017 Jan 10; Bogor (ID): Departemen Proteksi Tanaman, IPB University. hlm 27-35.

Karki, HS, Shrestha BK, Han JW, Groth DE, Barphagha IK, Rush MC, Melanson RA, Kim BS, Ham JH. 2012. Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae. Plos One. 7(9):1–12. DOI:

Khamna S, Yokota A, Peberdy JF, Lumyong S. 2010. Indole-3-Acetic-Acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. Eurasian Journal of Biosciences. 4:23–32. DOI:

Köberl M, Ramadan EM, Adam M, Cardinale M, Hallmann J, Heuer H, Smalla K, Berg G. 2013. Bacillus and Streptomyces were selected as broad-spectrum antagonists against soilborne pathogens from arid areas in Egypt. FEMS Microbiology Letters. 342(2):168–178. DOI:

Luo J, Xie G, Li B, Lihui X. 2007. First report of Burkholderia glumae isolated from symptomless rice seeds in China. Plant Disease. 91(10):1363. DOI:

Lu W, Pan L, Zao H, Jia Y, Wang Y, Yu X, Wang X. 2014. Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves. The Crop Journal. 2(6):398–406. DOI:

Madigan MT, Martinko JM, Parker J. 1997. Brock Biology of Microorganisms. Ed ke-8. New Jersey (US): Prentice-Hall.

Maeda Y, Kiba A, Ohnishi K, Hikichi Y. 2004. New method to detect oxolinic acid-resistant Burkholderia glumae infesting rice seeds using a mismatch amplification mutation assay polymerase chain reaction. Journal General Plant Pathology. 70:215–217. DOI:

Mizobuchi R, Fukuoka S, Tsushima S, Yano M, Sato H. 2016. QTLs for resistance to major rice diseases 571 exacerbated by global warming: brown spot, bacterial seedling rot, and bacterial grain rot. Rice. 9(1):1–2. DOI:

Mogi S, Wibowo BS, Zainuddin S. 1989. Laporan awal bacterial grain rot (Pseudomonas glumae) pada padi di Indonesia. Di dalam: Prosiding Kongres Nasional X dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia; 1989 Nov 14; Denpasar (ID): Perhimpunan Fitopatologi Indonesia.

Mondal KK, Mani C, Verma G. 2015. Emergence of bacterial panicle blight caused Burkholderia glumae in North India. Plant Disease. 99(9):1268. DOI:

Nandakumar R, Shahjahan AK, Yuan XL, Dickstein ER, Groth DE, Clark CA, Cartwright RD, Rush MC. 2009. Burkholderia glumae and B. gladioli cause bacterial panicle blight in rice in the southern United States. Plant Disease. 93(9):896–905. DOI:

Oskay MO, Tamer U, Azeri C. 2004. Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal Biotechnology. 3(9):441–446. DOI:

Patil HJ, Srivastava AK, Singh DP, Chaudhari BL, Arora DK. 2011. Actinomycetes mediated biochemical responses in tomato (Solanum lycopersicum) enhances bioprotection against Rhizoctonia solani. Crop Protection. 30(1):1269–1273. DOI:

Pedraza LA, Bautista J, Uribe-Vélez D. 2018. Seed-born Burkholderia glumae infects rice seedling and maintains bacterial population during vegetative and reproductive growth stage. The Plant Pathology Journal. 34(5):393–402. DOI:

Putra C, Giyanto. 2014. Kompatibilitas Bacillus spp. dan aktinomiset sebagai agens hayati Xanthomonas oryzae pv. oryzae dan pemacu pertumbuhan padi. Jurnal Fitopatologi Indonesia. 10(5):160–169. DOI:

Ruiz R, Vargas J, Quirolla C, Escobar M. 2014. First report Burkholderia glumae causing bacterial panicle blight on rice in Ecuador. Plant Disease. 98(7):988. DOI:

Sabaratnam S, Traquair JA. 2001. Formulation of a Streptomyces biocontrol agent for the supression of rhizoctonia damping-off in tomato transplant. of Biological Control. 23(3):245–253. DOI:

Schaad NW, Jones JB, Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. Minnesota (US): APS Press.

Solans M, Vobis G, Cassán F, Luna V, Wall LG. 2011. Production of phytohormones by root-associated saprophytic actinomycetes isolated from the actinorhizal plant Ochetophila trinervis. Journal Microbiology and Biotechnology. 27:2195–2202. DOI:

Todar K. 2008. Antimicrobial Agents Used in Treatment of Infection Disease [research report]. Madison (US): University of Wisconsin.

Watve MG, Tickoo R, Jog MM, Bhole BD. 2001. How many antibiotics are produced by the genus Streptomyces?. Archives of Microbiology. 176(5):386–390. DOI:

Wiyono S, Giyanto, Mutaqin KH, Hidayat SH, Supramana, Widodo. 2017. Emerging diseases pada tanaman pertanian: strategi dan opsi kebijakan pengendalian. Di dalam: Di dalam: Prosiding Simposium Nasional Fitopatologi di Indonesia “Kemunculan Penyakit Baru dan Impor Benih; 2017 Jan 10; Bogor (ID): Departemen Proteksi Tanaman, IPB University. hlm 8-15.

Yuvika, Nasution A, Gafur A. 2013. Isolasi dan penapisan in vitro aktinomiset untuk mengendalikan Xanthomonas. Jurnal Fitopatologi Indonesia. 9(5):160–164. DOI:

Zou XG. 2014. First report of bacterial panicle blight of rice cause by Burkholderia glumae in South Africa. Plant Disease. 98(4):566. DOI:

How to Cite
Nurmujahidin, GiyantoG., & Dadang. (2023). Pengendalian Hayati Penyakit Busuk Bulir Bakteri yang Disebabkan oleh Burkholderia glumae Menggunakan Aktinomiset. Jurnal Fitopatologi Indonesia, 19(2), 63-73.