Pythium ultimum and Phytopythium vexans, the Potential Pathogen Isolated from Potato Rhizosphere in Central Java, Indonesia
Abstract
Phytophthora and Pythium are a group of Oomycetes that are widely associated with diseases in potato plants. Therefore, this study was conducted to identify the Oomycetes associated with the rhizosphere of infected potato plants showing leaf blight. Four isolates were collected from four regions in Central Java (UGM_St_TM, UGM_St_BK, UGM_St_BNJ, and UGM_St_KJ, the isolate from Temanggung, Bakal, Banjarnegara, Kejajar respectively), and UGM_St_NG isolate as culture collection from Laboratory of Plant Pathology. Molecular identification of all isolates was carried out using the internal transcribed spacer (ITS1/ITS4), nuclear large-ribosomal subunit (LSU), and cytochrome C oxidase subunit 1 (COX1) gene markers. Based on the results, the isolates UGM_St_TM, UGM_St_BK, and UGM_St_BNJ were identified as Phythium ultimum while UGM_St_KJ and UGM_St_NG isolate were identified as Phytopythium vexans.
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Abad G, Burgess T, Bienapfi JC, Redford AJ, Coffey M, Knight L. 2019. Molecular and morphological identification of Phytophthora based on the types. https://idtools.org/id/phytophthora/morphology.php.
Asgar A. 2013. Kualitas umbi beberapa klon kentang (Solanum tuberosum L.) dataran medium untuk keripik. Berita Biologi. 12(1):29-37.
Bakkeren G, Kronstad JW, Le’vesque CA. 2000. Comparison of AFLP fingerprints and ITS sequences as phylogenetic markers in Ustilaginomycetes. Mycologia. 92(3):510–521. DOI: https://doi.org/10.2307/3761510.
Bala K, Robideau GP, Lévesque A, de Cock WAM, Abad ZG, Lodhi AM, Shahzad S, Ghaffar A, Coffey MD. 2010. Phytopythium sindhum Lodhi, Shahzad & Levesque, sp. nov. Persoonia. 24:136–137.
Baten MA, Li M, Motohashi K, Ishiguro Y, Rahman MZ, Suga H, Kageyama K. 2015. Two new species, Phytopythium iriomotense sp. nov. and P. aichiense sp. nov., isolated from river water and water purification sludge in Japan. Mycological progress. 14(2):1–2. DOI: https://doi.org/10.1007/s11557-015-1027-1.
Beakes GW, Honda D, Thines M. 2014. Beakes GW, Honda D, Thines M. 3 Systematics of the Straminipila: Labyrinthulomycota, Hyphochytriomycota, and Oomycota. In: Esser K, editor. Systematics and Evolution. Berlin (DE): Springer. pp. 39–97. DOI: https://doi.org/10.1007/978-3-642-55318-9_3.
de Cock AW, Lodhi AM, Rintoul TL, Bala K, Robideau GP, Abad ZG, Coffey MD, Shahzad S, Lévesque CA. 2015. Phytopythium: molecular phylogeny and systematics. Persoonia-Molecular Phylogeny and Evolution of Fungi. 34:25–39. DOI: https://doi.org/10.3767/003158515X685382.
Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus. 12:13–15. DOI: https://doi.org/10.2307/2419362.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 33(7):1870–1874. DOI: https://doi.org/10.1093/molbev/msw054.
Martin FN, Tooley PW. 2003. Phylogenetic relationships among Phytophthora species
inferred from sequence analysis of mitochondrially encoded cytochrome oxidase I and II genes. Mycologia. 95:269–284. DOI: https://doi.org/10.1080/15572536.2004.11833112.
Nam B, Choi YJ. 2019. Phytopythium and Pythium species (Oomycota) isolated from freshwater environments of Korea. Mycobiology. 47(3):261–272. DOI: https://doi.org/10.1080/12298093.2019.1625174.
Nathasia V, Abadi AL, Wardiyati T. 2014. Uji ketahanan 7 klon tanaman kentang (Solanum tuberosum L.) terhadap penyakit hawar daun (Phytopthora infestans (Mont.) de Barry). Jurnal Produksi Tanaman. 1(6):540–548.
Ochoa Fuentes YM, Cerna Chaves E, Gallegos Morales G, Landeros Flores J, Delgado Ortiz JC, Hernández Camacho S, Rodríguez Guerra R, Olalde Portugal V. 2012. Identificación de especies de Fusarium en semilla de ajo en Aguascalientes, México. Revista Mexicana de Micología. 36:27–31.
Polat Z, Awan QN, Hussain M, Akgül DS. 2017. First report of Phytopythium vexans causing root and collar rot of kiwifruit in Turkey. Plant Disease. 101:1058. DOI: https://doi.org/10.1094/PDIS-11-16-1554-PDN.
Radmer L, Anderson G, Malvick DM, Kurle JE, Rendahl A, Mallik A. 2017. Pythium, Phytophthora, and Phytopythium spp. associated with soybean in Minnesota, their relative aggressiveness on soybean and corn, and their sensitivity to seed treatment fungicides. Plant Disease. 101(1):62–72. DOI: https://doi.org/10.1094/PDIS-02-16-0196-RE.
Santika IA, Widiastuti A, Wibowo A. 2021. First report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque causing potato tuber rot in Indonesia. Jurnal Perlindungan Tanaman Indonesia. 25(2):173–181. DOI: https://doi.org/10.22146/jpti.67556.
Santoso PJ, Aryantha INP, Pancoro A, Suhandono S. 2015. Identification of Pythium and Phytophthora associated with Durian (Durio sp.) in Indonesia: Their molecular and morphological characteristics and distribution. Asian Journal of Plant Pathology. 9(2):59–71.
DOI: https://doi.org/10.3923/ajppaj.2015.59.71.
Schurko AM, Mendoza L, Lévesque CA, Desaulniers NL, de Cock AWAM, Klassen GR. 2003. A molecular phylogeny of Pythium insidiosum. Mycological Research. 107(5):537–544. DOI: https://doi.org/10.1017/S0953756203007718.
Thao L D, Hien LT, Liem NV, Thanh HM, Khanh TN, Binh VTP, Trang TTT, Anh PT, Tu TT. 2020. First report of Phytopythium vexans causing root rot disease on durian in Vietnam. New Disease Report. 41(1):2. DOI: https://doi.org/10.5197/j.2044-0588.2020.041.002.
Uzuhashi S, Tojo M, Kakishima M. 2010. Phylogeny of the genus Pythium and description of new genera. Mycoscience. 51(5):337–365. DOI: https://doi.org/10.1007/S10267-010-0046-7.
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