Plant-based coagulants for halal cheese production

  • Amiroh Auliya Rahma Faculty of Agriculture Technology, IPB University. Dramaga Campus IPB, Bogor, West Java, 16680, Indonesia
  • Azzahra Issustiarani Faculty of Agriculture Technology, IPB University. Dramaga Campus IPB, Bogor, West Java, 16680, Indonesia
Keywords: Cheese, Halal, Plant-based coagulants, Protease enzymes

Abstract

Advancements in cheese-making technology have successfully integrated traditional methods with contemporary innovations, enhancing efficiency, sustainability, and product quality. Traditional cheese production typically uses animal rennet, which is sourced from the stomachs of young ruminants. This rennet contains the enzymes chymosin and pepsin, which are essential for the milk coagulation process in cheese making. Due to the limited availability of animal rennet and the rising demand for cheese and halal food products, the market has expanded beyond Muslim consumers to include non-Muslim individuals, leading to the exploration of alternative plant-based coagulants. Therefore, this study aims to evaluate the potential use of plant-based coagulants in producing halal cheese while identifying the challenges associated with the production process. These challenges include proteolytic activity, which can lead to a bitter taste, and inconsistencies in coagulant quality due to variations in the sources of the plants. The current study focuses on different types of plant proteases, such as aspartate, cysteine, and serine, extracted from different parts, as well as protease production techniques. It also explores coagulant quality parameters, such as milk clotting activity, proteolytic activity, optimal temperature, and pH, as well as their effects on the physicochemical and organoleptic properties of cheese. The results are expected to provide comprehensive scientific insights for the development of effective alternative coagulants to meet the needs of the halal cheese market in the future.

References

Afsharnezhad M, Shahangian SS, Sariri R. A novel milk-clotting cysteine protease from Ficus johannis: Purification and characterization. International Journal of Biological Macromolecules. 2019;121:173-182. https://doi.org/10.1016/j.ijbiomac.2018.10.006

Aktayeva S, Akishev Z, Khassenov B. Proteolytic Enzymes in Cheese Making. Eurasian Journal of Applied Biotechnology. 2018;1:1-9. https://doi.org/10.11134/btp.1.2018.2

Alavi F, Momen S. Aspartic proteases from thistle flowers: Traditional coagulants used in the modern cheese industry. International Dairy Journal. 2020;107(104709). https://doi.org/10.1016/j.idairyj.2020.104709

Amaniyah M, Royanta Y, Baktian FM, Prayitno SS. Physicochemical and organoleptic changes in goat's milk cheddar cheese with the addition of Lactobacillus casei as a starter during ripening. IOP Publishing. 2024;1377(012083):1-8. https://doi.org/10.1088/1755-1315/1377/1/012083

Ariskanopitasari, Rizaldi RH, Sari RN. The exploration of indigenous plant-based coagulant for Sumbawanese cheese production as probiotic source. Jurnal Agrotek UMMAT. 2023;10(4):338-350. https://doi.org/10.31764/jau.v10i4.19514

Banik S, Biswas S, Karmakar S. Extraction, purification, and activity of protease from the leaves of Moringa oleifera. F1000Res. 2018;7(1151):1-13. https://doi.org/10.12688/f1000research.15642.1

Ben Amira A, Besbes S, Attia H, Blecker C. Milk-clotting properties of plant rennets and their enzymatic, rheological, and sensory role in cheese making: A review. International Journal of Food Properties. 2017;20(sup1):S76-S93. https://doi.org/10.1080/10942912.2017.1289959

Benalia A, Derbal K, Amrouci Z, Baatache O, Khalfaoui A, Pizzi A. Application of Plant-Based Coagulants and Their Mechanisms in Water Treatment: A Review. Journal of Renewable Materials. 2024;12(4):667-698. https://doi.org/10.32604/jrm.2024.048306

Bruno MA, Trejo SA, Aviles XF, Caffini NO, Lopez LMI. Isolation and characterization of hyeronymain II, another peptidase isolated from fruits of Bromelia hieronymi Mez (Bromeliaceae). Protein J. 2006;25(3):224-231. https://doi.org/10.1007/s10930-006-9005-8

Cipolat-Gotet C, Cecchinato A, De Marchi M, Bittante G. Factors affecting variation of different measures of cheese yield and milk nutrient recovery from an individual model cheese-manufacturing process. Journal of Dairy Science. 2013;96(12):7952-7965. https://doi.org/10.3168/jds.2012-6516

Delgado-Martinez FJ, Carrapiso AI, Contador R, Ramirez MR. Volatile compounds and sensory changes after high pressure processing of mature Torta del Casar (raw ewe's milk cheese) during refrigerated storage. Innovative Food Science & Emerging Technologies. 2019;52:34-41. https://doi.org/10.1016/j.ifset.2018.11.004

Esposito M, Di Pierro P, Dejonghe W, Mariniello L, Porta R. Enzymatic milk clotting activity in artichoke (Cynara scolymus) leaves and alpine thistle (Carduus defloratus) flowers. Immobilization of alpine thistle aspartic protease. Food chemistry. 2016;204:115-121. https://doi.org/10.1016/j.foodchem.2016.02.060

Freitas CDT, Leite HB, Oliveira JPB, Amaral JL, Egito AS, Vairo-Cavalli S, Lobo MDP, Monteiro-Moreira ACO, Ramos MV. Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora. Food Research International. 2016;87:50-59. https://doi.org/10.1016/j.foodres.2016.06.020

Grossmann L, McClements DJ. The science of plant-based foods: Approaches to create nutritious and sustainable plant-based cheese analogs. Trends in Food Science & Technology. 2021;118:207-229. https://doi.org/10.1016/j.tifs.2021.10.004

Hofer F, Kraml J, Kahler U, Kamenik AS, Liedi KR. Catalytic Site pKa Values of Aspartic, Cysteine, and Serine Proteases: Constant pH MD Simulations. J Chem Inf Model. 2020;60(6):3030-3042. https://doi.org/10.1021/acs.jcim.0c00190

Holyavka M, Faizullin D, Koroleva V, Olshannikova S, Zakhartchenko N, Zuev Y, Kondratyev M, Zakharova E, Artyukhov V. Novel biotechnological formulations of cysteine proteases, immobilized on chitosan. Structure, stability and activity. International Journal of Biological Macromolecules. 2021;180:161-176. https://doi.org/10.1016/j.ijbiomac.2021.03.016

Imran A, Alsayeqh A. Anticoccidial Efficacy of Citrus sinensis Essential Oil in Broiler Chicken. Pakistan Veterinary Journal. 2022;42(4). https://doi.org/10.29261/pakvetj/2022.082

Jacob M, Jaros D, Rohm H. Recent advances in milk clotting enzymes. International Journal of Dairy Technology. 2011;64(1):14-33. https://doi.org/10.1111/j.1471-0307.2010.00633.x

Kaur S, Huppertz T, Vasiljevic T. Plant proteases and their application in dairy systems. International Dairy Journal. 2024;154(105925):1-9. https://doi.org/10.1016/j.idairyj.2024.105925

Khan UM, Aadil RM, Shabbir MA, Shahid M, Decker EA. Interpreting the production, characterization and antioxidant potential of plant proteases. Food Science and Technology. 2023;43:e84922. https://doi.org/10.1590/fst.84922

Khan UM, Ahmad I, Inayat S, Amin HM A, Selamoglu Z. Physicochemical properties of Cheddar cheese made from Citrus reticulata Blanco crude flowers extract. Turkish Journal of Agriculture-Food Science and Technology. 2019;7(6):856-860. https://doi.org/10.24925/turjaf.v7i6.856-860.2391

Khan UM, Sameen A, Decker EA, Shabbir MA, Hussain S, Latif A, Abdi G, Aadil RM. Implementation of plant extracts for cheddar-type cheese production in conjunction with FTIR and Raman spectroscopy comparison. Food Chemistry: X. 2024;22:101256. https://doi.org/10.1016/j.fochx.2024.101256

Kuhfeld RF, Eshpari H, Atamer Z, Dallas DC. A comprehensive database of cheese-derived bitter peptides and correlation to their physical properties. Critical Reviews in Food Science and Nutrition. 2023;64(27):10105-10119. https://doi.org/10.1080/10408398.2023.2220792

Kumar A, Sasmal S. Rheological and physico-chemical properties of milk gel using isolate of pumpkin (Cucurbita moschata) seeds: A new source of milk clotting peptidase. Food Hydrocolloids. 2020;106(105866):1-8. https://doi.org/10.1016/j.foodhyd.2020.105866

Lee BH. Fundamentals of food biotechnology. Wiley-Blackwell, Cambridge. 2015. p. 433-483. https://doi.org/10.1002/9781118384947

Leulmi I, Zidoune MN, Hafid K, Djeghim F, Bourekoua H, Dziki D, Różyło R. New Coagulant Proteases for Cheese-making from Leaves and Latex of the Spontaneous Plant Pergularia tomentosa: Biochemical Characterization of Coagulants and Sensorial Evaluation of Cheese. Foods. 2023;12(2467):1-19. https://doi.org/10.3390/foods12132467

Marković S, Andrejević NS, Milošević J, Polović NĐ. Structural Transitions of Papain-like Cysteine Proteases: Implications for Sensor Development. Biomimetics. 2023;8(3):1-12. https://doi.org/10.3390/biomimetics8030281

Mazorra-Manzano MA, Moreno-Hernandez JM, Ramirez-Suarez JC. Biotechnological applications of plant proteolytic enzymes. Springer, Cham. 2018. p. 21-41. https://doi.org/10.1007/978-3-319-97132-2_2

Mazorra-Manzano MA, Perea-Gutiérrez TC, Lugo-Sánchez ME, Ramirez-Suarez JC, Torres-Llanez MJ, González-Córdova AF, Vallejo-Cordoba B. Comparison of the milk-clotting properties of three plant extracts. Food Chemistry. 2013;141(3):1902-1907. https://doi.org/10.1016/j.foodchem.2013.05.042

Mohsin AZ, Norsah E, Marzlan AA, Abd Rahim MH, Hussin ASM. Exploring the applications of plant-based coagulants in cheese production: A review. International Dairy Journal. 2024;148(105792):1-9. https://doi.org/10.1016/j.idairyj.2023.105792

Mureşan CC, Marc RA, Anamaria Semeniuc C, Ancuţa Socaci S, Fărcaş A, Fracisc D, Rodica Pop C, Rotar A, Dodan A, Mureşan V. Changes in Physicochemical and Microbiological Properties, Fatty Acid and Volatile Compound Profiles of Apuseni Cheese during Ripening. Foods. 2021;10(2):1-15. https://doi.org/10.3390/foods10020258

Murlidhar M, Anusha R, Bindhu OS. Plant-Based Coagulants in Cheese Making: Review. Apple Academic Press, Massachusetts. 2017. p. 3-35. https://doi.org/10.1201/9781315366210-2

Nasr AIAM, Ahmed IAM, Hamid OIA. Characterization of partially purified milk-clotting enzyme from sunflower (Helianthus annuus) seeds. Food Sciences and Nutrition. 2016;4(5):733-741. https://doi.org/10.1002/fsn3.338

Nicosia FD, Puglisi I, Pino A, Caggia C, Randazzo CL. Plant milk-clotting enzymes for cheese-making. Foods. 2022;11(871):1-16. https://doi.org/10.3390/foods11060871

Nurrachmi R. The global development of halal food industry: A survey. Tazkia Islamic Finance and Business Review. 2017;11(1):39-56. https://doi.org/10.30993/tifbr.v11i1.113

Ordiales E, Martin A, Benito MJ, Fernandez M, Casquete R, Cordoba MG. Influence of the technological properties of vegetable rennet (Cynara cardunculus) on the physicochemical, sensory and rheological characteristics of 'Torta del Casar' cheese. International Journal of Dairy Technology. 2014;67(3):402-409. https://doi.org/10.1111/1471-0307.12129

Ramadan II, Abd El-Wahed EM, Rabie AM, Baky AAA. Extraction, partial purification and some factors affecting milk clotting activity of a milk coagulant prepared from sunflower (Helianthus annus) seeds. Zagazic Journal of Agricultural Research. 2019;46(2):441-453. https://doi.org/10.21608/zjar.2019.33399

Ribeiro LR, Junior BRCL, Cristianini M. Effect of high-pressure processing on the characteristics of cheese made from ultrafiltered milk: Influence of the kind of rennet. Innovative Food Science & Emerging Technologies. 2018;50:57-65. https://doi.org/10.1016/j.ifset.2018.10.012

Roseiro LB, Barbosa M, Ames JM, Wilbey RA. Cheese-making with vegetable coagulants-the use of Cynara L. for the production of ovine milk cheeses. International Journal of Dairy Technology. 2003;56(2):76-85. https://doi.org/10.1046/j.1471-0307.2003.00080.x

Shah MA, Mir AS, Paray MA. Plant proteases as milk-clotting enzymes in cheese-making: a review. Dairy Sci. & Technol. 2014;94:5-16. https://doi.org/10.1007/s13594-013-0144-3

Sharma S, Kumar A, Kumar S, Katare AK, Bhat HF, Hassoun A, Aït-Kaddour A, Aadil RM, Mungure TE, Bhat ZF. Withania somnifera fruit extract is effective in controlling microbial growth and lipid oxidation and improves the functional value of cheese. Food Chemistry Advances. 2023;2:100204. https://doi.org/10.1016/j.focha.2023.100204

Singh BP, Bangar SP, Alblooshi M, Ajayi FF, Mudgil P, Maqsood S. Plant-derived proteins as a sustainable source of bioactive peptides: recent research updates on emerging production methods, bioactivities, and potential application. Critical Reviews in Food Science and Nutrition. 2023;63(28):9539-9560. https://doi.org/10.1080/10408398.2022.2067120

Tahir Z, Khan MI, Ashraf U, Adan IRD, Mubarik U. Industrial application of orange peel waste; a review. International Journal of Agriculture and Biosciences. 2023;12(2):71-76. https://doi.org/10.47278/journal.ijab/2023.046

Tripathi P, Tomar R, Jagannadham MV. Purification and biochemical characterisation of a novel protease streblin. Food Chemistry. 2011;125(3):1005-1012. https://doi.org/10.1016/j.foodchem.2010.09.108

Troncoso FD, Sánchez DA, Ferreira ML. Production of Plant Proteases and New Biotechnological Applications: An Updated Review. ChemistryOpen. 2022;11(3):1-38. https://doi.org/10.1002/open.202200017

Vidal JFD, Schwartz MF, Garay AV, Valadares NF, Bueno RV, Monteiro ACL, Freitas SMd, Barbosa JARG. Exploring the Diversity and Function of Serine Proteases in Toxicofera Reptile Venoms: A Comprehensive Overview. Toxins. 2024;16(10):1-38. https://doi.org/10.3390/toxins16100428

Wang X, Shi Y, He R, Li B, Huang A. Label-free quantitative proteomic analysis of the biological functions of Moringa oleifera seed proteins provides insights regarding the milk-clotting proteases. International Journal of Biological Macromolecules. 2020;144:325-333. https://doi.org/10.1016/j.ijbiomac.2019.12.070

Yano H, Fu W. Effective use of plant proteins for the development of "new" foods. Foods. 2022;11(9):1185. https://doi.org/10.3390/foods11091185

Yegin S, Dekker P. Progress in the field of aspartic proteinases in cheese manufacturing: structures, functions, catalytic mechanism, inhibition, and engineering. Dairy Sci. & Technol. 2013;93:565-594. https://doi.org/10.1007/s13594-013-0137-2

Zikiou A, Zidoune MN. Enzymatic extract from flowers of Algerian spontaneous Cynara cardunculus: Milk-clotting properties and use in the manufacture of a Camembert-type cheese. International Journal of Dairy Technology. 2019;72(1):89-99. https://doi.org/10.1111/1471-0307.12563

Published
2024-12-03
How to Cite
RahmaA. A., & IssustiaraniA. (2024). Plant-based coagulants for halal cheese production. Halal Studies and Society, 2(1), 14-17. https://doi.org/10.29244/hass.2.1.14-17
Section
Short Communications